This pipeline uses various statistical tests to identify genes whose promoter methylation levels correlated to selected clinical features.
Testing the association between 20233 genes and 8 clinical features across 292 samples, statistically thresholded by Q value < 0.05, 7 clinical features related to at least one genes.
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1 gene correlated to 'Time to Death'.
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CYP51A1
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2 genes correlated to 'AGE'.
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RIPPLY2 , PBX4
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4 genes correlated to 'GENDER'.
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KIF4B , UTP14C , MGC21881 , FRG1B
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1 gene correlated to 'PATHOLOGY.T'.
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PRO1768
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2 genes correlated to 'PATHOLOGY.N'.
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CD74 , VAMP8
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1 gene correlated to 'TUMOR.STAGE'.
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IPO7
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1 gene correlated to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
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PTPN1
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No genes correlated to 'NEOADJUVANT.THERAPY'
Complete statistical result table is provided in Supplement Table 1
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
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Time to Death | Cox regression test | N=1 | shorter survival | N=1 | longer survival | N=0 |
AGE | Spearman correlation test | N=2 | older | N=2 | younger | N=0 |
GENDER | t test | N=4 | male | N=2 | female | N=2 |
PATHOLOGY T | Spearman correlation test | N=1 | higher pT | N=0 | lower pT | N=1 |
PATHOLOGY N | Spearman correlation test | N=2 | higher pN | N=2 | lower pN | N=0 |
TUMOR STAGE | Spearman correlation test | N=1 | higher stage | N=1 | lower stage | N=0 |
RADIATIONS RADIATION REGIMENINDICATION | t test | N=1 | yes | N=0 | no | N=1 |
NEOADJUVANT THERAPY | t test | N=0 |
Time to Death | Duration (Months) | 0.1-210.9 (median=14.2) |
censored | N = 169 | |
death | N = 121 | |
Significant markers | N = 1 | |
associated with shorter survival | 1 | |
associated with longer survival | 0 |
HazardRatio | Wald_P | Q | C_index | |
---|---|---|---|---|
CYP51A1 | 7501 | 9.455e-07 | 0.019 | 0.525 |
AGE | Mean (SD) | 61.29 (12) |
Significant markers | N = 2 | |
pos. correlated | 2 | |
neg. correlated | 0 |
GENDER | Labels | N |
FEMALE | 82 | |
MALE | 210 | |
Significant markers | N = 4 | |
Higher in MALE | 2 | |
Higher in FEMALE | 2 |
T(pos if higher in 'MALE') | ttestP | Q | AUC | |
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KIF4B | -8.24 | 8.995e-14 | 1.82e-09 | 0.7829 |
UTP14C | 7.74 | 5.51e-12 | 1.11e-07 | 0.7997 |
MGC21881 | 5.37 | 2.911e-07 | 0.00589 | 0.6824 |
FRG1B | -5.3 | 6.541e-07 | 0.0132 | 0.6764 |
PATHOLOGY.T | Mean (SD) | 2.93 (1) |
N | ||
T1 | 21 | |
T2 | 76 | |
T3 | 61 | |
T4 | 99 | |
Significant markers | N = 1 | |
pos. correlated | 0 | |
neg. correlated | 1 |
SpearmanCorr | corrP | Q | |
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PRO1768 | -0.2907 | 2.128e-06 | 0.0431 |
PATHOLOGY.N | Mean (SD) | 1.04 (0.96) |
N | ||
N0 | 96 | |
N1 | 31 | |
N2 | 97 | |
N3 | 4 | |
Significant markers | N = 2 | |
pos. correlated | 2 | |
neg. correlated | 0 |
TUMOR.STAGE | Mean (SD) | 3.3 (0.97) |
N | ||
Stage 1 | 15 | |
Stage 2 | 46 | |
Stage 3 | 40 | |
Stage 4 | 151 | |
Significant markers | N = 1 | |
pos. correlated | 1 | |
neg. correlated | 0 |
SpearmanCorr | corrP | Q | |
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IPO7 | 0.2969 | 1.594e-06 | 0.0322 |
One gene related to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
RADIATIONS.RADIATION.REGIMENINDICATION | Labels | N |
NO | 78 | |
YES | 214 | |
Significant markers | N = 1 | |
Higher in YES | 0 | |
Higher in NO | 1 |
T(pos if higher in 'YES') | ttestP | Q | AUC | |
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PTPN1 | -5.02 | 1.596e-06 | 0.0323 | 0.6936 |
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Expresson data file = HNSC.meth.for_correlation.filtered_data.txt
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Clinical data file = HNSC.clin.merged.picked.txt
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Number of patients = 292
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Number of genes = 20233
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Number of clinical features = 8
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.