Analysis Overview for Brain Lower Grade Glioma
(primary solid tumor cohort)
Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)
Overview
Introduction

This is the analysis overview for Firehose run "16 January 2013".

Summary

Note: These results are offered to the community as an additional reference point, enabling a wide range of cancer biologists, clinical investigators, and genome and computational scientists to easily incorporate TCGA into the backdrop of ongoing research. While every effort is made to ensure that Firehose input data and algorithms are of the highest possible quality, these analyses have not been reviewed by domain experts.

Results

  • Sequence and Copy Number Analyses

    • Copy number analysis (GISTIC2)
      View Report | There were 180 tumor samples used in this analysis: 21 significant arm-level results, 13 significant focal amplifications, and 23 significant focal deletions were found.

    • Mutation Analysis (MutSig v2.0)
      View Report | 

    • Mutation Analysis (MutSig vS2N)
      View Report | 

  • Clustering Analyses

    • Clustering of copy number data: consensus NMF
      View Report | The most robust consensus NMF clustering of 180 samples using the 36 copy number focal regions was identified for k = 3 clusters. We computed the clustering for k = 2 to k = 8 and used the cophenetic correlation coefficient to determine the best solution.

    • Clustering of Methylation: consensus NMF
      View Report | The 8481 most variable methylated genes were selected based on variation. The variation cutoff are set for each tumor type empirically by fitting a bimodal distriution. For genes with multiple methylation probes, we chose the most variable one to represent the gene. Consensus NMF clustering of 176 samples and 8481 genes identified 4 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

    • Clustering of mRNA expression: consensus NMF
      View Report | The most robust consensus NMF clustering of 27 samples using the 1500 most variable genes was identified for k = 3 clusters. We computed the clustering for k = 2 to k = 8 and used the cophenetic correlation coefficient to determine the best solution.

    • Clustering of mRNA expression: consensus hierarchical
      View Report | The 1500 most variable genes were selected. Consensus average linkage hierarchical clustering of 27 samples and 1500 genes identified 3 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

    • Clustering of mRNAseq gene expression: consensus NMF
      View Report | The most robust consensus NMF clustering of 110 samples using the 1500 most variable genes was identified for k = 4 clusters. We computed the clustering for k = 2 to k = 8 and used the cophenetic correlation coefficient to determine the best solution.

    • Clustering of mRNAseq gene expression: consensus hierarchical
      View Report | The 1500 most variable genes were selected. Consensus average linkage hierarchical clustering of 110 samples and 1500 genes identified 4 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

    • Clustering of miRseq expression: consensus NMF
      View Report | We filtered the data to 150 most variable miRs. Consensus NMF clustering of 180 samples and 150 miRs identified 7 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

    • Clustering of miRseq expression: consensus hierarchical
      View Report | We filtered the data to 150 most variable miRs. Consensus average linkage hierarchical clustering of 180 samples and 150 miRs identified 3 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

  • Correlation Analyses

    • Correlation between copy number variations of arm-level result and selected clinical features
      View Report | Testing the association between copy number variation 49 arm-level results and 6 clinical features across 178 patients, 14 significant findings detected with Q value < 0.25.

    • Correlation between copy number variation genes and selected clinical features
      View Report | Testing the association between copy number variation of 36 peak regions and 6 clinical features across 178 patients, 13 significant findings detected with Q value < 0.25.

    • Correlation between gene methylation status and clinical features
      View Report | Testing the association between 17352 genes and 6 clinical features across 104 samples, statistically thresholded by Q value < 0.05, 4 clinical features related to at least one genes.

    • Correlation between molecular cancer subtypes and selected clinical features
      View Report | Testing the association between subtypes identified by 8 different clustering approaches and 6 clinical features across 179 patients, 12 significant findings detected with P value < 0.05 and Q value < 0.25.

    • Correlation between gene mutation status and selected clinical features
      View Report | Testing the association between mutation status of 51 genes and 6 clinical features across 168 patients, 10 significant findings detected with Q value < 0.25.

    • Correlation between mRNA expression and clinical features
      View Report | Testing the association between 17814 genes and 6 clinical features across 27 samples, statistically thresholded by Q value < 0.05, 2 clinical features related to at least one genes.

    • Correlation between mRNAseq expression and clinical features
      View Report | Testing the association between 18308 genes and 6 clinical features across 110 samples, statistically thresholded by Q value < 0.05, 5 clinical features related to at least one genes.

    • Correlation between miRseq expression and clinical features
      View Report | Testing the association between 560 genes and 6 clinical features across 178 samples, statistically thresholded by Q value < 0.05, 4 clinical features related to at least one genes.

    • Correlations between copy number and mRNA expression
      View Report | The correlation coefficients in 10, 20, 30, 40, 50, 60, 70, 80, 90 percentiles are -0.16948, -0.04906, 0.04202, 0.1253, 0.2063, 0.2897, 0.3785, 0.4823, 0.6175, respectively.

    • Correlations between copy number and mRNAseq expression
      View Report | The correlation coefficients in 10, 20, 30, 40, 50, 60, 70, 80, 90 percentiles are 780, 1903, 2437, 2972, 3502, 4086, 4747, 5516, 6518, respectively.

    • Correlation between mRNA expression and DNA methylation
      View Report | The top 25 correlated methylation probes per gene are displayed. Total number of matched samples = 106. Number of gene expression samples = 110. Number of methylation samples = 106.

  • Other Analyses

    • HotNet pathway analysis of mutation and copy number data
      View Report | There were 28 significant subnetworks identified in HotNet analysis.

    • PARADIGM pathway analysis of mRNA expression data
      View Report | There were 36 significant pathways identified in this analysis.

    • PARADIGM pathway analysis of mRNA expression and copy number data
      View Report | There were 29 significant pathways identified in this analysis.

    • PARADIGM pathway analysis of mRNASeq expression data
      View Report | There were 60 significant pathways identified in this analysis.

    • PARADIGM pathway analysis of mRNASeq expression and copy number data
      View Report | There were 56 significant pathways identified in this analysis.

    • Association of mutation, copy number alteration, and subtype markers with pathways
      View Report | There are 28 genes with significant mutation (Q value <= 0.1) and 348 genes with significant copy number alteration (Q value <= 0.25). The identified marker genes (Q value <= 0.01 or within top 2000) are 245 for subtype 1, 245 for subtype 2, 245 for subtype 3. Pathways significantly enriched with these genes (Q value <= 0.01) are identified :

    • The boxplot mRNA array based expression data
      View Report

Methods & Data
Input

  • Run Prefix = analyses__2013_01_16

  • Summary Report Date = Mon Feb 11 17:58:28 2013

  • Protection = FALSE

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