(primary solid tumor cohort)
This pipeline computes the correlation between cancer subtypes identified by different molecular patterns and selected clinical features.
Testing the association between subtypes identified by 4 different clustering approaches and 3 clinical features across 62 patients, one significant finding detected with P value < 0.05 and Q value < 0.25.
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3 subtypes identified in current cancer cohort by 'CN CNMF'. These subtypes do not correlate to any clinical features.
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3 subtypes identified in current cancer cohort by 'METHLYATION CNMF'. These subtypes do not correlate to any clinical features.
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CNMF clustering analysis on sequencing-based miR expression data identified 3 subtypes that do not correlate to any clinical features.
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Consensus hierarchical clustering analysis on sequencing-based miR expression data identified 4 subtypes that correlate to 'AGE'.
Clinical Features |
Time to Death |
AGE | GENDER |
Statistical Tests | logrank test | ANOVA | Fisher's exact test |
CN CNMF |
0.751 (1.00) |
0.871 (1.00) |
0.495 (1.00) |
METHLYATION CNMF |
0.199 (1.00) |
0.154 (1.00) |
0.469 (1.00) |
MIRseq CNMF subtypes |
0.943 (1.00) |
0.242 (1.00) |
0.158 (1.00) |
MIRseq cHierClus subtypes |
0.706 (1.00) |
0.0122 (0.146) |
0.262 (1.00) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 15 | 22 | 24 |
P value = 0.751 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 55 | 26 | 0.1 - 90.7 (12.8) |
subtype1 | 14 | 7 | 0.1 - 90.7 (7.8) |
subtype2 | 19 | 8 | 0.4 - 69.6 (14.3) |
subtype3 | 22 | 11 | 0.3 - 83.6 (11.3) |
P value = 0.871 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 56 | 61.8 (14.0) |
subtype1 | 14 | 60.4 (16.0) |
subtype2 | 19 | 61.5 (15.0) |
subtype3 | 23 | 62.9 (12.3) |
P value = 0.495 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 22 | 39 |
subtype1 | 4 | 11 |
subtype2 | 10 | 12 |
subtype3 | 8 | 16 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 19 | 15 | 27 |
P value = 0.199 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 54 | 25 | 0.1 - 90.7 (12.2) |
subtype1 | 15 | 8 | 0.4 - 90.7 (19.8) |
subtype2 | 13 | 7 | 0.1 - 53.3 (7.1) |
subtype3 | 26 | 10 | 0.3 - 83.6 (8.3) |
P value = 0.154 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 56 | 60.8 (14.7) |
subtype1 | 15 | 57.7 (18.8) |
subtype2 | 15 | 56.9 (15.8) |
subtype3 | 26 | 64.9 (10.2) |
P value = 0.469 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 23 | 38 |
subtype1 | 9 | 10 |
subtype2 | 6 | 9 |
subtype3 | 8 | 19 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 22 | 12 | 27 |
P value = 0.943 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 54 | 25 | 0.1 - 83.6 (12.2) |
subtype1 | 19 | 11 | 0.1 - 69.6 (14.4) |
subtype2 | 9 | 4 | 1.1 - 83.6 (5.9) |
subtype3 | 26 | 10 | 0.3 - 53.3 (11.0) |
P value = 0.242 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 56 | 61.0 (14.8) |
subtype1 | 21 | 57.2 (14.9) |
subtype2 | 9 | 59.7 (19.4) |
subtype3 | 26 | 64.5 (12.7) |
P value = 0.158 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 23 | 38 |
subtype1 | 11 | 11 |
subtype2 | 2 | 10 |
subtype3 | 10 | 17 |
Cluster Labels | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Number of samples | 8 | 9 | 20 | 24 |
P value = 0.706 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 54 | 25 | 0.1 - 83.6 (12.2) |
subtype1 | 8 | 2 | 0.3 - 53.3 (2.3) |
subtype2 | 7 | 4 | 1.1 - 83.6 (11.6) |
subtype3 | 17 | 11 | 0.1 - 69.6 (14.9) |
subtype4 | 22 | 8 | 2.6 - 51.2 (14.0) |
P value = 0.0122 (ANOVA), Q value = 0.15
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 56 | 61.0 (14.8) |
subtype1 | 8 | 64.2 (6.5) |
subtype2 | 7 | 60.7 (18.5) |
subtype3 | 19 | 52.6 (16.8) |
subtype4 | 22 | 67.2 (10.7) |
P value = 0.262 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 23 | 38 |
subtype1 | 1 | 7 |
subtype2 | 2 | 7 |
subtype3 | 9 | 11 |
subtype4 | 11 | 13 |
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Cluster data file = LIHC-TP.mergedcluster.txt
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Clinical data file = LIHC-TP.clin.merged.picked.txt
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Number of patients = 62
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Number of clustering approaches = 4
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Number of selected clinical features = 3
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Exclude small clusters that include fewer than K patients, K = 3
consensus non-negative matrix factorization clustering approach (Brunet et al. 2004)
Resampling-based clustering method (Monti et al. 2003)
For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R
For continuous numerical clinical features, one-way analysis of variance (Howell 2002) was applied to compare the clinical values between tumor subtypes using 'anova' function in R
For binary clinical features, two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.