Colon/Rectal Adenocarcinoma: Correlation between gene mutation status and selected clinical features<BR>(primary solid tumor cohort)

Colon/Rectal Adenocarcinoma: Correlation between gene mutation status and selected clinical features
(primary solid tumor cohort)

Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)

Overview

Introduction

This pipeline computes the correlation between significantly recurrent gene mutations and selected clinical features.

Summary

Testing the association between mutation status of 209 genes and 11 clinical features across 224 patients, 29 significant findings detected with Q value < 0.25.

PIK3CA mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
BRAF mutation correlated to 'HISTOLOGICAL.TYPE'.
KRTAP5-5 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
NRXN1 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
CNTN6 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
CDH2 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
CNBD1 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
ERBB4 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
ACVR2A mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
CDH9 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
GRIK3 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
GGT1 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
PCDHA10 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
FAM5C mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
IFT80 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
DACH2 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
PCDH11X mutation correlated to 'Time to Death'.
BTNL8 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
NXF5 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
SPATA17 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
ZCWPW2 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
CDH12 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
ZNF167 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
ZFPM2 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
DPY19L1 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
TMPRSS13 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
B2M mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
RNASE11 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.
COL6A6 mutation correlated to 'NUMBER.OF.LYMPH.NODES'.

Results

Overview of the results

Table 1. Get Full Table Overview of the association between mutation status of 209 genes and 11 clinical features. Shown in the table are P values (Q values). Thresholded by Q value < 0.25, 29 significant findings detected.


		Clinical Features	Time to Death	AGE	PRIMARY SITE OF DISEASE	GENDER	HISTOLOGICAL TYPE	PATHOLOGY T	PATHOLOGY N	PATHOLOGICSPREAD(M)	TUMOR STAGE	COMPLETENESS OF RESECTION	NUMBER OF LYMPH NODES
	nMutated (%)	nWild-Type	logrank test	t-test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	t-test
PIK3CA	33 (15%)	191	0.767 (1.00)	0.0855 (1.00)	0.227 (1.00)	0.851 (1.00)	0.0168 (1.00)	0.913 (1.00)	0.0235 (1.00)	0.133 (1.00)	0.0358 (1.00)	0.303 (1.00)	1.26e-05 (0.0285)
BRAF	22 (10%)	202	0.912 (1.00)	0.0839 (1.00)	0.0261 (1.00)	0.0702 (1.00)	2e-06 (0.00451)	0.191 (1.00)	0.904 (1.00)	0.395 (1.00)	0.576 (1.00)	0.611 (1.00)	0.589 (1.00)
KRTAP5-5	4 (2%)	220		0.882 (1.00)	0.588 (1.00)	1 (1.00)	0.305 (1.00)	1 (1.00)	0.478 (1.00)	1 (1.00)	0.151 (1.00)	1 (1.00)	3.21e-11 (7.29e-08)
NRXN1	20 (9%)	204	0.682 (1.00)	0.907 (1.00)	0.133 (1.00)	0.819 (1.00)	0.594 (1.00)	0.192 (1.00)	0.0571 (1.00)	0.53 (1.00)	0.134 (1.00)	0.604 (1.00)	2.98e-05 (0.0671)
CNTN6	17 (8%)	207	0.45 (1.00)	0.161 (1.00)	0.102 (1.00)	0.206 (1.00)	0.386 (1.00)	0.124 (1.00)	0.135 (1.00)	0.206 (1.00)	0.165 (1.00)	0.0753 (1.00)	9.11e-05 (0.205)
CDH2	16 (7%)	208	0.592 (1.00)	0.302 (1.00)	0.781 (1.00)	0.445 (1.00)	0.703 (1.00)	0.306 (1.00)	0.03 (1.00)	0.0244 (1.00)	0.00766 (1.00)	0.376 (1.00)	8.45e-06 (0.0191)
CNBD1	7 (3%)	217	0.525 (1.00)	0.617 (1.00)	0.679 (1.00)	0.712 (1.00)	0.796 (1.00)	0.735 (1.00)	0.436 (1.00)	0.61 (1.00)	0.43 (1.00)	0.637 (1.00)	5.88e-05 (0.132)
ERBB4	18 (8%)	206	0.0646 (1.00)	0.686 (1.00)	0.595 (1.00)	0.624 (1.00)	0.615 (1.00)	0.0896 (1.00)	0.0133 (1.00)	0.206 (1.00)	0.0527 (1.00)	0.389 (1.00)	5.02e-08 (0.000114)
ACVR2A	9 (4%)	215	0.271 (1.00)	0.454 (1.00)	0.282 (1.00)	0.0902 (1.00)	0.717 (1.00)	0.9 (1.00)	0.352 (1.00)	1 (1.00)	0.682 (1.00)	1 (1.00)	3.99e-08 (9.03e-05)
CDH9	15 (7%)	209	0.0266 (1.00)	0.218 (1.00)	0.766 (1.00)	0.6 (1.00)	0.815 (1.00)	0.145 (1.00)	0.0166 (1.00)	0.717 (1.00)	0.00616 (1.00)	0.366 (1.00)	1.74e-10 (3.95e-07)
GRIK3	17 (8%)	207	0.754 (1.00)	0.0446 (1.00)	0.283 (1.00)	0.135 (1.00)	0.617 (1.00)	0.852 (1.00)	0.135 (1.00)	1 (1.00)	0.159 (1.00)	1 (1.00)	0.000109 (0.246)
GGT1	3 (1%)	221		0.966 (1.00)	0.555 (1.00)	0.607 (1.00)		0.698 (1.00)	0.778 (1.00)	1 (1.00)	0.748 (1.00)	1 (1.00)	3.24e-11 (7.36e-08)
PCDHA10	10 (4%)	214	0.096 (1.00)	0.474 (1.00)	0.727 (1.00)	0.525 (1.00)	0.282 (1.00)	0.0532 (1.00)	0.139 (1.00)	1 (1.00)	0.595 (1.00)	1 (1.00)	3.1e-07 (7e-04)
FAM5C	13 (6%)	211	0.0736 (1.00)	0.51 (1.00)	0.759 (1.00)	0.153 (1.00)	0.51 (1.00)	0.0974 (1.00)	0.0339 (1.00)	0.267 (1.00)	0.115 (1.00)	0.458 (1.00)	2.7e-10 (6.12e-07)
IFT80	10 (4%)	214	0.945 (1.00)	0.689 (1.00)	1 (1.00)	1 (1.00)	0.622 (1.00)	0.383 (1.00)	0.139 (1.00)	0.393 (1.00)	0.246 (1.00)	0.653 (1.00)	8.54e-10 (1.94e-06)
DACH2	12 (5%)	212	0.365 (1.00)	0.551 (1.00)	1 (1.00)	0.557 (1.00)	0.94 (1.00)	0.0578 (1.00)	0.199 (1.00)	1 (1.00)	0.893 (1.00)	1 (1.00)	5.01e-05 (0.113)
PCDH11X	14 (6%)	210	6.96e-05 (0.157)	0.546 (1.00)	0.559 (1.00)	0.787 (1.00)	0.611 (1.00)	0.0393 (1.00)	0.0862 (1.00)	0.269 (1.00)	0.233 (1.00)	0.113 (1.00)	0.011 (1.00)
BTNL8	3 (1%)	221		0.0123 (1.00)	0.555 (1.00)	0.607 (1.00)		0.698 (1.00)	0.778 (1.00)	1 (1.00)	0.748 (1.00)	1 (1.00)	3.24e-11 (7.36e-08)
NXF5	7 (3%)	217	0.261 (1.00)	0.171 (1.00)	0.44 (1.00)	0.263 (1.00)	0.558 (1.00)	0.268 (1.00)	0.143 (1.00)	0.61 (1.00)	0.15 (1.00)	0.637 (1.00)	3.11e-11 (7.07e-08)
SPATA17	9 (4%)	215	0.525 (1.00)	0.735 (1.00)	0.725 (1.00)	0.74 (1.00)	0.0298 (1.00)	0.0106 (1.00)	0.197 (1.00)	0.392 (1.00)	0.388 (1.00)	0.653 (1.00)	1.4e-06 (0.00317)
ZCWPW2	7 (3%)	217	0.441 (1.00)	0.346 (1.00)	0.44 (1.00)	0.712 (1.00)	0.555 (1.00)	0.0501 (1.00)	0.143 (1.00)	1 (1.00)	0.378 (1.00)	0.593 (1.00)	3.11e-11 (7.07e-08)
CDH12	13 (6%)	211	0.398 (1.00)	0.0595 (1.00)	0.543 (1.00)	0.777 (1.00)	0.815 (1.00)	0.223 (1.00)	0.0339 (1.00)	0.267 (1.00)	0.145 (1.00)	0.469 (1.00)	1.32e-08 (2.98e-05)
ZNF167	7 (3%)	217	0.376 (1.00)	0.538 (1.00)	0.679 (1.00)	1 (1.00)	0.796 (1.00)	0.239 (1.00)	0.436 (1.00)	1 (1.00)	0.183 (1.00)	1 (1.00)	1.5e-08 (3.4e-05)
ZFPM2	6 (3%)	218	0.376 (1.00)	0.193 (1.00)	0.374 (1.00)	0.215 (1.00)	0.437 (1.00)	0.17 (1.00)	0.535 (1.00)	0.609 (1.00)	0.666 (1.00)	1 (1.00)	1.03e-07 (0.000233)
DPY19L1	5 (2%)	219	0.405 (1.00)	0.61 (1.00)	0.643 (1.00)	0.672 (1.00)	0.639 (1.00)	0.0723 (1.00)	0.217 (1.00)	1 (1.00)	0.273 (1.00)	1 (1.00)	3.18e-11 (7.22e-08)
TMPRSS13	4 (2%)	220		0.26 (1.00)	1 (1.00)	0.351 (1.00)	1 (1.00)	0.237 (1.00)	0.478 (1.00)	1 (1.00)	0.458 (1.00)	1 (1.00)	3.21e-11 (7.29e-08)
B2M	5 (2%)	219	0.375 (1.00)	0.851 (1.00)	0.643 (1.00)	0.195 (1.00)	0.783 (1.00)	0.127 (1.00)	0.692 (1.00)	1 (1.00)	0.86 (1.00)	1 (1.00)	1.67e-06 (0.00378)
RNASE11	5 (2%)	219	0.365 (1.00)	0.189 (1.00)	0.327 (1.00)	0.672 (1.00)	0.495 (1.00)	0.056 (1.00)	0.217 (1.00)	1 (1.00)	0.367 (1.00)	1 (1.00)	3.18e-11 (7.22e-08)
COL6A6	16 (7%)	208	0.671 (1.00)	0.845 (1.00)	0.157 (1.00)	0.201 (1.00)	0.415 (1.00)	0.143 (1.00)	0.03 (1.00)	0.721 (1.00)	0.0553 (1.00)	0.376 (1.00)	1.98e-07 (0.000447)
FBXW7	38 (17%)	186	0.696 (1.00)	0.0555 (1.00)	0.341 (1.00)	0.374 (1.00)	0.0135 (1.00)	0.103 (1.00)	0.777 (1.00)	0.0041 (1.00)	0.00151 (1.00)	0.0342 (1.00)	0.855 (1.00)
NRAS	20 (9%)	204	0.0691 (1.00)	0.0434 (1.00)	0.8 (1.00)	0.0365 (1.00)	1 (1.00)	0.277 (1.00)	0.0375 (1.00)	0.377 (1.00)	0.765 (1.00)	0.469 (1.00)	0.0251 (1.00)
APC	160 (71%)	64	0.368 (1.00)	0.404 (1.00)	0.0159 (1.00)	0.0753 (1.00)	0.119 (1.00)	0.91 (1.00)	0.915 (1.00)	0.661 (1.00)	0.409 (1.00)	0.191 (1.00)	0.899 (1.00)
KRAS	96 (43%)	128	0.0237 (1.00)	0.113 (1.00)	0.027 (1.00)	0.685 (1.00)	0.0636 (1.00)	0.351 (1.00)	0.317 (1.00)	0.203 (1.00)	0.584 (1.00)	0.0373 (1.00)	0.194 (1.00)
SMAD4	26 (12%)	198	0.795 (1.00)	0.987 (1.00)	1 (1.00)	0.837 (1.00)	0.00552 (1.00)	0.81 (1.00)	1 (1.00)	0.788 (1.00)	0.793 (1.00)	0.83 (1.00)	0.318 (1.00)
TP53	120 (54%)	104	0.496 (1.00)	0.0605 (1.00)	0.0135 (1.00)	0.284 (1.00)	0.000245 (0.55)	0.874 (1.00)	0.279 (1.00)	0.919 (1.00)	0.241 (1.00)	0.559 (1.00)	0.467 (1.00)
FAM123B	25 (11%)	199	0.634 (1.00)	0.863 (1.00)	0.5 (1.00)	1 (1.00)	0.726 (1.00)	0.644 (1.00)	0.332 (1.00)	0.782 (1.00)	0.585 (1.00)	0.645 (1.00)	0.498 (1.00)
TTN	85 (38%)	139	0.592 (1.00)	0.392 (1.00)	0.00419 (1.00)	0.409 (1.00)	0.0035 (1.00)	0.111 (1.00)	0.0598 (1.00)	0.0671 (1.00)	0.284 (1.00)	0.905 (1.00)	0.371 (1.00)
SMAD2	15 (7%)	209	0.885 (1.00)	0.713 (1.00)	0.779 (1.00)	0.293 (1.00)	0.482 (1.00)	0.333 (1.00)	0.269 (1.00)	1 (1.00)	0.244 (1.00)	0.746 (1.00)	0.0341 (1.00)
TCF7L2	18 (8%)	206	0.282 (1.00)	0.404 (1.00)	0.785 (1.00)	0.624 (1.00)	0.44 (1.00)	0.299 (1.00)	0.943 (1.00)	0.737 (1.00)	0.885 (1.00)	0.573 (1.00)	0.108 (1.00)
FAT4	39 (17%)	185	0.58 (1.00)	0.152 (1.00)	0.567 (1.00)	0.725 (1.00)	0.464 (1.00)	0.473 (1.00)	0.486 (1.00)	0.0388 (1.00)	0.154 (1.00)	0.0414 (1.00)	0.971 (1.00)
ACVR1B	14 (6%)	210	0.605 (1.00)	0.831 (1.00)	0.0692 (1.00)	1 (1.00)	0.0288 (1.00)	0.034 (1.00)	1 (1.00)	0.717 (1.00)	0.391 (1.00)	1 (1.00)	0.477 (1.00)
WBSCR17	19 (8%)	205	0.39 (1.00)	0.242 (1.00)	0.0659 (1.00)	1 (1.00)	0.296 (1.00)	0.79 (1.00)	0.841 (1.00)	0.522 (1.00)	0.119 (1.00)	0.102 (1.00)	0.172 (1.00)
PCDH9	22 (10%)	202	0.128 (1.00)	0.382 (1.00)	0.812 (1.00)	0.123 (1.00)	0.932 (1.00)	0.464 (1.00)	0.779 (1.00)	0.387 (1.00)	0.0429 (1.00)	0.191 (1.00)	0.985 (1.00)
LRP1B	39 (17%)	185	0.816 (1.00)	0.213 (1.00)	0.341 (1.00)	0.29 (1.00)	0.0115 (1.00)	0.478 (1.00)	0.779 (1.00)	0.83 (1.00)	0.943 (1.00)	0.758 (1.00)	0.806 (1.00)
TNFRSF10C	6 (3%)	218		0.838 (1.00)	0.181 (1.00)	0.685 (1.00)	0.395 (1.00)	0.0674 (1.00)	0.193 (1.00)	0.064 (1.00)	0.0117 (1.00)	0.0793 (1.00)	0.184 (1.00)
MAP2K4	11 (5%)	213	0.0342 (1.00)	0.119 (1.00)	0.51 (1.00)	0.761 (1.00)	0.754 (1.00)	0.347 (1.00)	0.694 (1.00)	0.402 (1.00)	0.271 (1.00)	0.668 (1.00)	0.104 (1.00)
KIAA1804	15 (7%)	209	0.284 (1.00)	0.799 (1.00)	0.0177 (1.00)	0.425 (1.00)	0.00911 (1.00)	0.726 (1.00)	0.344 (1.00)	1 (1.00)	0.881 (1.00)	0.746 (1.00)	0.0129 (1.00)
KLHL4	16 (7%)	208	0.129 (1.00)	0.118 (1.00)	0.781 (1.00)	0.118 (1.00)	0.119 (1.00)	0.0111 (1.00)	0.719 (1.00)	0.199 (1.00)	0.233 (1.00)	0.0753 (1.00)	0.837 (1.00)
SOX9	10 (4%)	214	0.573 (1.00)	0.266 (1.00)	0.29 (1.00)	0.525 (1.00)	0.581 (1.00)	0.158 (1.00)	0.902 (1.00)	1 (1.00)	0.64 (1.00)	1 (1.00)	0.203 (1.00)
PRDM9	16 (7%)	208	0.249 (1.00)	0.325 (1.00)	1 (1.00)	0.606 (1.00)	0.813 (1.00)	0.215 (1.00)	0.363 (1.00)	0.0124 (1.00)	0.79 (1.00)	0.389 (1.00)	0.135 (1.00)
PCBP1	6 (3%)	218	0.557 (1.00)	0.767 (1.00)	1 (1.00)	0.685 (1.00)	1 (1.00)	0.498 (1.00)	0.535 (1.00)	0.0311 (1.00)	0.378 (1.00)	1 (1.00)	0.0129 (1.00)
DMD	33 (15%)	191	0.364 (1.00)	0.566 (1.00)	0.105 (1.00)	0.132 (1.00)	0.0182 (1.00)	0.423 (1.00)	0.115 (1.00)	0.375 (1.00)	0.0188 (1.00)	0.607 (1.00)	0.107 (1.00)
CSMD1	26 (12%)	198	0.00687 (1.00)	0.854 (1.00)	0.654 (1.00)	0.677 (1.00)	0.778 (1.00)	0.392 (1.00)	0.362 (1.00)	1 (1.00)	0.254 (1.00)	0.377 (1.00)	0.000554 (1.00)
LIFR	18 (8%)	206	0.0294 (1.00)	0.692 (1.00)	1 (1.00)	0.227 (1.00)	0.741 (1.00)	0.317 (1.00)	0.943 (1.00)	1 (1.00)	0.929 (1.00)	1 (1.00)	0.852 (1.00)
OR6N1	10 (4%)	214	0.847 (1.00)	0.573 (1.00)	0.727 (1.00)	0.751 (1.00)	0.926 (1.00)	0.706 (1.00)	0.54 (1.00)	1 (1.00)	0.963 (1.00)	0.397 (1.00)	0.749 (1.00)
CCDC160	7 (3%)	217	0.535 (1.00)	0.631 (1.00)	1 (1.00)	0.263 (1.00)	1 (1.00)	0.0671 (1.00)	0.436 (1.00)	1 (1.00)	0.865 (1.00)	1 (1.00)	0.0598 (1.00)
CPXCR1	9 (4%)	215	0.243 (1.00)	0.311 (1.00)	1 (1.00)	0.0902 (1.00)	0.434 (1.00)	0.209 (1.00)	0.0702 (1.00)	0.392 (1.00)	0.565 (1.00)	0.0466 (1.00)	0.000567 (1.00)
SLITRK1	15 (7%)	209	0.898 (1.00)	0.956 (1.00)	0.779 (1.00)	0.112 (1.00)	0.813 (1.00)	0.0487 (1.00)	0.269 (1.00)	0.276 (1.00)	0.328 (1.00)	0.366 (1.00)	0.0034 (1.00)
CTNNB1	11 (5%)	213	0.586 (1.00)	0.253 (1.00)	0.74 (1.00)	0.359 (1.00)	0.543 (1.00)	0.175 (1.00)	0.31 (1.00)	1 (1.00)	0.753 (1.00)	1 (1.00)	0.00374 (1.00)
DKK2	6 (3%)	218	0.678 (1.00)	0.452 (1.00)	0.669 (1.00)	1 (1.00)	0.763 (1.00)	0.648 (1.00)	0.0876 (1.00)	1 (1.00)	0.598 (1.00)	0.593 (1.00)	0.167 (1.00)
FLG	32 (14%)	192	0.222 (1.00)	0.892 (1.00)	0.207 (1.00)	1 (1.00)	0.0293 (1.00)	0.44 (1.00)	0.18 (1.00)	0.368 (1.00)	0.351 (1.00)	0.482 (1.00)	0.0167 (1.00)
ARID1A	21 (9%)	203	0.142 (1.00)	0.573 (1.00)	0.621 (1.00)	0.492 (1.00)	0.0894 (1.00)	0.126 (1.00)	0.0963 (1.00)	0.387 (1.00)	0.114 (1.00)	0.611 (1.00)	0.0682 (1.00)
KIAA1486	13 (6%)	211	0.18 (1.00)	0.404 (1.00)	1 (1.00)	1 (1.00)	0.366 (1.00)	0.316 (1.00)	1 (1.00)	0.269 (1.00)	0.52 (1.00)	0.102 (1.00)	0.771 (1.00)
ROBO1	18 (8%)	206	0.767 (1.00)	0.733 (1.00)	0.595 (1.00)	0.471 (1.00)	0.0293 (1.00)	0.0847 (1.00)	0.622 (1.00)	1 (1.00)	0.226 (1.00)	1 (1.00)	0.0279 (1.00)
ZFHX4	24 (11%)	200	0.00119 (1.00)	0.949 (1.00)	1 (1.00)	0.289 (1.00)	0.544 (1.00)	0.0374 (1.00)	0.151 (1.00)	0.395 (1.00)	0.27 (1.00)	0.509 (1.00)	0.406 (1.00)
EPHA3	16 (7%)	208	0.495 (1.00)	0.562 (1.00)	0.402 (1.00)	0.3 (1.00)	0.613 (1.00)	0.285 (1.00)	0.543 (1.00)	1 (1.00)	0.851 (1.00)	0.746 (1.00)	0.306 (1.00)
PRIM2	8 (4%)	216	0.0794 (1.00)	0.852 (1.00)	0.703 (1.00)	0.724 (1.00)	0.752 (1.00)	0.105 (1.00)	0.325 (1.00)	1 (1.00)	0.636 (1.00)	1 (1.00)	0.000806 (1.00)
UBE2NL	7 (3%)	217	0.399 (1.00)	0.212 (1.00)	1 (1.00)	0.712 (1.00)	0.896 (1.00)	0.13 (1.00)	0.436 (1.00)	0.609 (1.00)	0.943 (1.00)	1 (1.00)	0.0598 (1.00)
ADAM29	11 (5%)	213	0.272 (1.00)	0.996 (1.00)	0.51 (1.00)	0.123 (1.00)	0.932 (1.00)	0.221 (1.00)	0.694 (1.00)	0.0511 (1.00)	0.317 (1.00)	1 (1.00)	0.968 (1.00)
ATP10A	19 (8%)	205	0.686 (1.00)	0.52 (1.00)	1 (1.00)	0.811 (1.00)	0.107 (1.00)	0.0309 (1.00)	0.289 (1.00)	0.53 (1.00)	0.0565 (1.00)	0.604 (1.00)	0.16 (1.00)
DNAH5	34 (15%)	190	0.722 (1.00)	0.664 (1.00)	0.105 (1.00)	0.578 (1.00)	0.394 (1.00)	0.248 (1.00)	0.251 (1.00)	1 (1.00)	0.244 (1.00)	0.863 (1.00)	0.37 (1.00)
GABRA5	9 (4%)	215	0.153 (1.00)	0.35 (1.00)	0.282 (1.00)	0.74 (1.00)	0.717 (1.00)	0.308 (1.00)	0.803 (1.00)	1 (1.00)	1 (1.00)	1 (1.00)	0.13 (1.00)
NALCN	20 (9%)	204	0.582 (1.00)	0.61 (1.00)	1 (1.00)	0.819 (1.00)	0.846 (1.00)	0.828 (1.00)	0.21 (1.00)	0.522 (1.00)	0.102 (1.00)	1 (1.00)	0.293 (1.00)
OTOL1	8 (4%)	216	0.365 (1.00)	0.104 (1.00)	0.44 (1.00)	1 (1.00)	0.682 (1.00)	0.0185 (1.00)	0.528 (1.00)	0.619 (1.00)	0.444 (1.00)	0.643 (1.00)	0.166 (1.00)
PTEN	7 (3%)	217	0.513 (1.00)	0.0301 (1.00)	0.44 (1.00)	0.712 (1.00)	0.262 (1.00)	0.167 (1.00)	1 (1.00)	1 (1.00)	0.792 (1.00)	0.593 (1.00)	0.14 (1.00)
SDK1	27 (12%)	197	0.729 (1.00)	0.393 (1.00)	0.00362 (1.00)	0.685 (1.00)	0.00901 (1.00)	0.977 (1.00)	0.314 (1.00)	0.796 (1.00)	0.886 (1.00)	0.525 (1.00)	0.816 (1.00)
TLL1	15 (7%)	209	0.292 (1.00)	0.212 (1.00)	1 (1.00)	0.6 (1.00)	0.144 (1.00)	0.666 (1.00)	0.931 (1.00)	0.196 (1.00)	0.278 (1.00)	0.0849 (1.00)	0.0344 (1.00)
TMEM132D	19 (8%)	205	0.807 (1.00)	0.879 (1.00)	0.441 (1.00)	0.811 (1.00)	0.188 (1.00)	0.844 (1.00)	1 (1.00)	0.158 (1.00)	0.173 (1.00)	0.325 (1.00)	0.609 (1.00)
TRPC4	17 (8%)	207	0.571 (1.00)	0.369 (1.00)	1 (1.00)	0.45 (1.00)	0.0666 (1.00)	0.0127 (1.00)	0.0451 (1.00)	0.518 (1.00)	0.65 (1.00)	1 (1.00)	0.000999 (1.00)
ATM	25 (11%)	199	0.827 (1.00)	0.709 (1.00)	0.11 (1.00)	0.0934 (1.00)	0.262 (1.00)	0.76 (1.00)	0.666 (1.00)	0.299 (1.00)	0.399 (1.00)	0.509 (1.00)	0.848 (1.00)
GPC6	10 (4%)	214	0.463 (1.00)	0.995 (1.00)	1 (1.00)	1 (1.00)	0.235 (1.00)	0.0354 (1.00)	1 (1.00)	0.652 (1.00)	0.884 (1.00)	0.448 (1.00)	0.518 (1.00)
CDH8	14 (6%)	210	0.458 (1.00)	0.31 (1.00)	1 (1.00)	1 (1.00)	1 (1.00)	0.895 (1.00)	1 (1.00)	0.437 (1.00)	0.842 (1.00)	0.726 (1.00)	0.325 (1.00)
MGC26647	7 (3%)	217	0.67 (1.00)	0.976 (1.00)	0.103 (1.00)	0.263 (1.00)	0.174 (1.00)	0.13 (1.00)	0.501 (1.00)	0.61 (1.00)	0.658 (1.00)	0.637 (1.00)	0.0751 (1.00)
DKK4	7 (3%)	217	0.574 (1.00)	0.256 (1.00)	0.44 (1.00)	1 (1.00)	0.156 (1.00)	0.735 (1.00)	0.436 (1.00)	1 (1.00)	0.755 (1.00)	1 (1.00)	0.000217 (0.487)
PTPRM	20 (9%)	204	0.245 (1.00)	0.672 (1.00)	1 (1.00)	0.493 (1.00)	0.467 (1.00)	0.299 (1.00)	0.68 (1.00)	0.53 (1.00)	0.258 (1.00)	0.774 (1.00)	0.839 (1.00)
AFF2	17 (8%)	207	0.855 (1.00)	0.778 (1.00)	0.102 (1.00)	0.622 (1.00)	0.0776 (1.00)	0.518 (1.00)	0.687 (1.00)	0.206 (1.00)	0.29 (1.00)	0.376 (1.00)	0.0486 (1.00)
NCAM2	12 (5%)	212	0.684 (1.00)	0.654 (1.00)	0.52 (1.00)	1 (1.00)	0.14 (1.00)	0.0395 (1.00)	0.653 (1.00)	1 (1.00)	0.718 (1.00)	1 (1.00)	0.865 (1.00)
OR2M4	8 (4%)	216	0.636 (1.00)	0.444 (1.00)	0.11 (1.00)	0.484 (1.00)	0.196 (1.00)	0.781 (1.00)	1 (1.00)	1 (1.00)	0.956 (1.00)	0.643 (1.00)	0.586 (1.00)
RYR2	29 (13%)	195	0.817 (1.00)	0.927 (1.00)	0.519 (1.00)	0.43 (1.00)	0.799 (1.00)	0.167 (1.00)	0.82 (1.00)	0.803 (1.00)	0.64 (1.00)	0.346 (1.00)	0.934 (1.00)
ELF3	6 (3%)	218	0.678 (1.00)	0.202 (1.00)	0.374 (1.00)	0.685 (1.00)	0.841 (1.00)	0.194 (1.00)	1 (1.00)	1 (1.00)	0.792 (1.00)	0.593 (1.00)	0.626 (1.00)
TRPS1	17 (8%)	207	0.536 (1.00)	0.246 (1.00)	0.595 (1.00)	0.622 (1.00)	0.308 (1.00)	0.0966 (1.00)	0.0646 (1.00)	0.199 (1.00)	0.0488 (1.00)	0.376 (1.00)	0.0103 (1.00)
FAM133A	7 (3%)	217	0.4 (1.00)	0.791 (1.00)	1 (1.00)	0.712 (1.00)	0.32 (1.00)	0.639 (1.00)	1 (1.00)	1 (1.00)	0.89 (1.00)	0.593 (1.00)	0.773 (1.00)
IL1RAPL2	10 (4%)	214	0.47 (1.00)	0.817 (1.00)	0.18 (1.00)	0.2 (1.00)	0.0261 (1.00)	0.768 (1.00)	1 (1.00)	0.393 (1.00)	0.441 (1.00)	0.668 (1.00)	0.533 (1.00)
CCBP2	10 (4%)	214	0.459 (1.00)	0.901 (1.00)	0.18 (1.00)	0.525 (1.00)	0.144 (1.00)	0.221 (1.00)	0.235 (1.00)	1 (1.00)	0.408 (1.00)	1 (1.00)	0.000112 (0.251)
OPCML	10 (4%)	214	0.3 (1.00)	0.726 (1.00)	0.0717 (1.00)	1 (1.00)	0.126 (1.00)	0.325 (1.00)	1 (1.00)	1 (1.00)	0.723 (1.00)	0.653 (1.00)	0.891 (1.00)
CDH18	13 (6%)	211	0.59 (1.00)	0.405 (1.00)	0.759 (1.00)	0.395 (1.00)	0.898 (1.00)	0.242 (1.00)	0.488 (1.00)	0.717 (1.00)	0.746 (1.00)	1 (1.00)	0.00222 (1.00)
OR2L13	10 (4%)	214	0.661 (1.00)	0.871 (1.00)	1 (1.00)	0.751 (1.00)	1 (1.00)	0.917 (1.00)	0.479 (1.00)	1 (1.00)	0.487 (1.00)	0.153 (1.00)	0.107 (1.00)
LRRN3	10 (4%)	214	0.868 (1.00)	0.486 (1.00)	0.29 (1.00)	0.525 (1.00)	0.581 (1.00)	0.353 (1.00)	0.0795 (1.00)	0.0511 (1.00)	0.276 (1.00)	1 (1.00)	0.304 (1.00)
BAI3	18 (8%)	206	0.85 (1.00)	0.0982 (1.00)	0.794 (1.00)	1 (1.00)	0.0773 (1.00)	0.0799 (1.00)	0.118 (1.00)	1 (1.00)	0.0963 (1.00)	1 (1.00)	0.606 (1.00)
MGC42105	11 (5%)	213	0.555 (1.00)	0.641 (1.00)	0.179 (1.00)	0.761 (1.00)	0.454 (1.00)	0.297 (1.00)	0.46 (1.00)	0.392 (1.00)	0.702 (1.00)	1 (1.00)	0.0238 (1.00)
TXNDC3	11 (5%)	213	0.378 (1.00)	0.541 (1.00)	0.179 (1.00)	0.359 (1.00)	0.454 (1.00)	0.563 (1.00)	0.763 (1.00)	0.402 (1.00)	0.656 (1.00)	0.668 (1.00)	0.225 (1.00)
LPPR4	13 (6%)	211	0.623 (1.00)	0.249 (1.00)	0.353 (1.00)	0.777 (1.00)	0.36 (1.00)	0.157 (1.00)	0.0443 (1.00)	1 (1.00)	0.952 (1.00)	1 (1.00)	0.0149 (1.00)
UNC13C	17 (8%)	207	0.71 (1.00)	0.901 (1.00)	1 (1.00)	0.206 (1.00)	0.574 (1.00)	0.091 (1.00)	0.169 (1.00)	0.518 (1.00)	0.275 (1.00)	0.767 (1.00)	0.535 (1.00)
SCN7A	15 (7%)	209	0.709 (1.00)	0.648 (1.00)	0.562 (1.00)	0.791 (1.00)	0.611 (1.00)	0.00602 (1.00)	0.099 (1.00)	1 (1.00)	0.14 (1.00)	0.726 (1.00)	0.972 (1.00)
ACOT4	3 (1%)	221		0.0265 (1.00)	0.555 (1.00)	1 (1.00)	0.399 (1.00)	0.0608 (1.00)	1 (1.00)	0.376 (1.00)	0.489 (1.00)	0.36 (1.00)	0.653 (1.00)
GRIA1	15 (7%)	209	0.458 (1.00)	0.554 (1.00)	0.0419 (1.00)	0.181 (1.00)	0.231 (1.00)	0.454 (1.00)	0.931 (1.00)	0.0694 (1.00)	0.582 (1.00)	0.746 (1.00)	0.211 (1.00)
ING1	6 (3%)	218	0.113 (1.00)	0.606 (1.00)	0.669 (1.00)	0.429 (1.00)	0.355 (1.00)	0.434 (1.00)	0.0109 (1.00)	1 (1.00)	0.118 (1.00)	1 (1.00)	0.547 (1.00)
ZNF208	11 (5%)	213	0.506 (1.00)	0.102 (1.00)	1 (1.00)	1 (1.00)	0.823 (1.00)	0.0272 (1.00)	0.833 (1.00)	0.191 (1.00)	0.463 (1.00)	0.233 (1.00)	0.581 (1.00)
CRTC1	6 (3%)	218		0.0365 (1.00)	0.669 (1.00)	0.429 (1.00)	0.763 (1.00)	0.194 (1.00)	0.535 (1.00)	0.609 (1.00)	0.746 (1.00)	1 (1.00)	0.000473 (1.00)
GUCY1A3	12 (5%)	212	0.209 (1.00)	0.0494 (1.00)	0.352 (1.00)	0.771 (1.00)	0.146 (1.00)	0.207 (1.00)	0.917 (1.00)	0.402 (1.00)	0.297 (1.00)	0.458 (1.00)	0.834 (1.00)
FAM22F	9 (4%)	215	0.199 (1.00)	0.401 (1.00)	0.282 (1.00)	0.00356 (1.00)	0.717 (1.00)	0.00563 (1.00)	1 (1.00)	0.12 (1.00)	0.0653 (1.00)	0.164 (1.00)	0.602 (1.00)
CHRDL1	11 (5%)	213	0.934 (1.00)	0.634 (1.00)	0.74 (1.00)	0.761 (1.00)	0.875 (1.00)	0.248 (1.00)	1 (1.00)	0.402 (1.00)	0.612 (1.00)	1 (1.00)	0.37 (1.00)
EDNRB	10 (4%)	214	0.413 (1.00)	0.196 (1.00)	0.5 (1.00)	0.338 (1.00)	0.803 (1.00)	0.0983 (1.00)	0.182 (1.00)	0.393 (1.00)	0.388 (1.00)	1 (1.00)	0.00275 (1.00)
ANK2	30 (13%)	194	0.142 (1.00)	0.597 (1.00)	0.206 (1.00)	0.33 (1.00)	0.275 (1.00)	0.071 (1.00)	0.926 (1.00)	0.804 (1.00)	0.558 (1.00)	0.288 (1.00)	0.125 (1.00)
CASP14	8 (4%)	216	0.455 (1.00)	0.766 (1.00)	0.253 (1.00)	1 (1.00)	0.707 (1.00)	0.695 (1.00)	0.0674 (1.00)	0.281 (1.00)	0.0347 (1.00)	0.288 (1.00)	0.478 (1.00)
LASS3	9 (4%)	215	0.61 (1.00)	0.532 (1.00)	1 (1.00)	0.74 (1.00)	0.842 (1.00)	0.0408 (1.00)	0.197 (1.00)	0.619 (1.00)	0.428 (1.00)	0.0879 (1.00)	0.00303 (1.00)
P2RY10	7 (3%)	217	0.0115 (1.00)	0.541 (1.00)	0.679 (1.00)	1 (1.00)	0.496 (1.00)	0.239 (1.00)	0.436 (1.00)	0.609 (1.00)	0.442 (1.00)	0.106 (1.00)	0.00237 (1.00)
CNTN1	14 (6%)	210	0.823 (1.00)	0.642 (1.00)	1 (1.00)	0.416 (1.00)	0.898 (1.00)	0.0101 (1.00)	1 (1.00)	1 (1.00)	0.619 (1.00)	0.746 (1.00)	0.575 (1.00)
ISL1	8 (4%)	216	0.607 (1.00)	0.858 (1.00)	0.11 (1.00)	0.724 (1.00)	0.196 (1.00)	0.781 (1.00)	0.115 (1.00)	1 (1.00)	0.328 (1.00)	1 (1.00)	0.737 (1.00)
ARID2	15 (7%)	209	0.901 (1.00)	0.831 (1.00)	1 (1.00)	0.791 (1.00)	1 (1.00)	0.284 (1.00)	0.606 (1.00)	1 (1.00)	0.164 (1.00)	0.746 (1.00)	0.048 (1.00)
EIF4A2	5 (2%)	219		0.0793 (1.00)	1 (1.00)	0.672 (1.00)	1 (1.00)	0.513 (1.00)	0.692 (1.00)	0.546 (1.00)	0.456 (1.00)	0.527 (1.00)	0.044 (1.00)
OR51V1	9 (4%)	215	0.453 (1.00)	0.68 (1.00)	0.06 (1.00)	0.316 (1.00)	0.00253 (1.00)	0.0338 (1.00)	0.282 (1.00)	0.392 (1.00)	0.0206 (1.00)	0.653 (1.00)	0.244 (1.00)
ZC3H13	21 (9%)	203	0.578 (1.00)	0.114 (1.00)	0.321 (1.00)	1 (1.00)	0.0754 (1.00)	0.0664 (1.00)	0.654 (1.00)	0.168 (1.00)	0.294 (1.00)	0.347 (1.00)	0.115 (1.00)
CDH11	14 (6%)	210	0.418 (1.00)	0.736 (1.00)	0.0692 (1.00)	0.416 (1.00)	0.309 (1.00)	0.895 (1.00)	0.589 (1.00)	1 (1.00)	0.775 (1.00)	0.726 (1.00)	0.234 (1.00)
CHST9	8 (4%)	216	0.131 (1.00)	0.553 (1.00)	0.703 (1.00)	0.724 (1.00)	0.0593 (1.00)	0.248 (1.00)	0.89 (1.00)	1 (1.00)	0.865 (1.00)	0.637 (1.00)	0.307 (1.00)
HTR3B	6 (3%)	218	0.552 (1.00)	0.604 (1.00)	1 (1.00)	0.685 (1.00)	0.655 (1.00)	0.17 (1.00)	0.733 (1.00)	0.609 (1.00)	0.705 (1.00)	1 (1.00)	0.945 (1.00)
LRRIQ3	10 (4%)	214	0.504 (1.00)	0.883 (1.00)	1 (1.00)	0.0509 (1.00)	1 (1.00)	0.17 (1.00)	1 (1.00)	1 (1.00)	0.91 (1.00)	1 (1.00)	0.888 (1.00)
HCN1	10 (4%)	214	0.295 (1.00)	0.416 (1.00)	0.727 (1.00)	1 (1.00)	0.754 (1.00)	0.308 (1.00)	0.479 (1.00)	1 (1.00)	0.487 (1.00)	1 (1.00)	0.0453 (1.00)
CHRM2	10 (4%)	214	0.428 (1.00)	0.835 (1.00)	0.727 (1.00)	0.525 (1.00)	0.754 (1.00)	0.0154 (1.00)	0.902 (1.00)	0.191 (1.00)	0.0321 (1.00)	0.233 (1.00)	0.598 (1.00)
CSMD3	28 (12%)	196	0.348 (1.00)	0.967 (1.00)	0.83 (1.00)	0.842 (1.00)	0.0114 (1.00)	0.0282 (1.00)	0.233 (1.00)	0.803 (1.00)	0.0408 (1.00)	1 (1.00)	0.00368 (1.00)
DCAF4L2	11 (5%)	213	0.938 (1.00)	0.557 (1.00)	0.51 (1.00)	1 (1.00)	0.0596 (1.00)	0.0145 (1.00)	0.694 (1.00)	0.393 (1.00)	0.398 (1.00)	0.668 (1.00)	0.224 (1.00)
DOCK2	21 (9%)	203	0.626 (1.00)	0.914 (1.00)	0.621 (1.00)	1 (1.00)	0.457 (1.00)	0.191 (1.00)	0.344 (1.00)	0.168 (1.00)	0.173 (1.00)	0.347 (1.00)	0.981 (1.00)
KCNA4	12 (5%)	212	0.934 (1.00)	0.629 (1.00)	0.352 (1.00)	0.557 (1.00)	0.146 (1.00)	0.643 (1.00)	0.599 (1.00)	0.267 (1.00)	0.575 (1.00)	0.458 (1.00)	0.281 (1.00)
DPP10	13 (6%)	211	0.848 (1.00)	0.409 (1.00)	1 (1.00)	0.777 (1.00)	0.366 (1.00)	0.281 (1.00)	0.41 (1.00)	0.269 (1.00)	0.52 (1.00)	0.484 (1.00)	0.431 (1.00)
EVC2	17 (8%)	207	0.324 (1.00)	0.397 (1.00)	0.595 (1.00)	1 (1.00)	0.518 (1.00)	0.595 (1.00)	0.731 (1.00)	0.728 (1.00)	0.263 (1.00)	0.389 (1.00)	0.663 (1.00)
C15ORF2	12 (5%)	212	0.503 (1.00)	0.637 (1.00)	1 (1.00)	0.38 (1.00)	0.27 (1.00)	0.307 (1.00)	0.07 (1.00)	0.402 (1.00)	0.197 (1.00)	0.668 (1.00)	0.0716 (1.00)
NLRP5	12 (5%)	212	0.702 (1.00)	0.485 (1.00)	0.352 (1.00)	0.38 (1.00)	0.735 (1.00)	0.444 (1.00)	0.252 (1.00)	0.697 (1.00)	0.424 (1.00)	0.469 (1.00)	0.605 (1.00)
KLK2	3 (1%)	221		0.179 (1.00)	0.555 (1.00)	1 (1.00)	0.399 (1.00)	1 (1.00)	1 (1.00)	1 (1.00)	0.863 (1.00)	1 (1.00)	0.561 (1.00)
LUZP2	6 (3%)	218		0.819 (1.00)	1 (1.00)	0.215 (1.00)	0.247 (1.00)	0.689 (1.00)	0.364 (1.00)	0.609 (1.00)	0.943 (1.00)	1 (1.00)	0.0463 (1.00)
PCDHB5	14 (6%)	210	0.0851 (1.00)	0.678 (1.00)	0.236 (1.00)	0.271 (1.00)	0.709 (1.00)	0.249 (1.00)	0.504 (1.00)	1 (1.00)	0.164 (1.00)	1 (1.00)	0.00149 (1.00)
LOXL2	9 (4%)	215	0.405 (1.00)	0.54 (1.00)	1 (1.00)	0.175 (1.00)	0.916 (1.00)	0.667 (1.00)	1 (1.00)	0.619 (1.00)	0.789 (1.00)	0.637 (1.00)	0.393 (1.00)
UBQLNL	5 (2%)	219		0.199 (1.00)	0.643 (1.00)	0.372 (1.00)	0.577 (1.00)	0.783 (1.00)	0.566 (1.00)	1 (1.00)	0.897 (1.00)	1 (1.00)	0.414 (1.00)
MYO1B	13 (6%)	211	0.343 (1.00)	0.632 (1.00)	1 (1.00)	0.777 (1.00)	0.366 (1.00)	0.673 (1.00)	0.53 (1.00)	0.267 (1.00)	0.575 (1.00)	0.458 (1.00)	0.123 (1.00)
RIMS1	15 (7%)	209	0.803 (1.00)	0.833 (1.00)	0.562 (1.00)	0.6 (1.00)	0.613 (1.00)	0.584 (1.00)	0.133 (1.00)	0.276 (1.00)	0.156 (1.00)	0.484 (1.00)	0.0211 (1.00)
COL12A1	21 (9%)	203	0.339 (1.00)	0.846 (1.00)	1 (1.00)	1 (1.00)	0.165 (1.00)	0.259 (1.00)	0.0963 (1.00)	0.168 (1.00)	0.149 (1.00)	0.315 (1.00)	0.447 (1.00)
JAKMIP2	13 (6%)	211	0.258 (1.00)	0.267 (1.00)	0.759 (1.00)	0.395 (1.00)	0.51 (1.00)	0.172 (1.00)	0.281 (1.00)	0.269 (1.00)	0.297 (1.00)	0.469 (1.00)	0.000711 (1.00)
CDH10	14 (6%)	210	0.803 (1.00)	0.668 (1.00)	0.236 (1.00)	0.416 (1.00)	0.431 (1.00)	0.454 (1.00)	0.372 (1.00)	0.717 (1.00)	0.647 (1.00)	1 (1.00)	0.009 (1.00)
EPHA5	13 (6%)	211	0.931 (1.00)	0.192 (1.00)	1 (1.00)	1 (1.00)	0.624 (1.00)	0.791 (1.00)	0.926 (1.00)	0.267 (1.00)	0.622 (1.00)	0.458 (1.00)	0.184 (1.00)
ODZ1	22 (10%)	202	0.00351 (1.00)	0.435 (1.00)	0.621 (1.00)	0.00017 (0.381)	0.0346 (1.00)	0.921 (1.00)	0.951 (1.00)	0.77 (1.00)	0.697 (1.00)	0.604 (1.00)	0.177 (1.00)
POSTN	11 (5%)	213	0.0234 (1.00)	0.794 (1.00)	0.02 (1.00)	1 (1.00)	0.024 (1.00)	1 (1.00)	0.255 (1.00)	0.393 (1.00)	0.251 (1.00)	0.653 (1.00)	0.365 (1.00)
SYNE1	47 (21%)	177	0.351 (1.00)	0.379 (1.00)	0.154 (1.00)	0.626 (1.00)	0.0767 (1.00)	0.0304 (1.00)	0.163 (1.00)	0.322 (1.00)	0.456 (1.00)	0.803 (1.00)	0.0549 (1.00)
ZEB2	15 (7%)	209	0.354 (1.00)	0.987 (1.00)	1 (1.00)	0.6 (1.00)	0.959 (1.00)	0.376 (1.00)	0.524 (1.00)	0.721 (1.00)	0.643 (1.00)	0.758 (1.00)	0.969 (1.00)
TUSC3	7 (3%)	217	0.405 (1.00)	0.509 (1.00)	1 (1.00)	1 (1.00)	0.896 (1.00)	0.498 (1.00)	0.501 (1.00)	0.61 (1.00)	0.658 (1.00)	0.637 (1.00)	0.631 (1.00)
CACNG7	6 (3%)	218	0.33 (1.00)	0.869 (1.00)	0.374 (1.00)	0.429 (1.00)	0.437 (1.00)	1 (1.00)	0.535 (1.00)	0.609 (1.00)	0.666 (1.00)	1 (1.00)	0.0129 (1.00)
RWDD2B	8 (4%)	216	0.796 (1.00)	0.247 (1.00)	0.703 (1.00)	1 (1.00)	0.824 (1.00)	0.115 (1.00)	0.325 (1.00)	1 (1.00)	0.334 (1.00)	1 (1.00)	0.000806 (1.00)
SLC25A13	7 (3%)	217	0.651 (1.00)	0.178 (1.00)	1 (1.00)	0.122 (1.00)	0.896 (1.00)	0.0501 (1.00)	0.501 (1.00)	0.61 (1.00)	0.658 (1.00)	0.637 (1.00)	0.0751 (1.00)
DPF2	4 (2%)	220		0.527 (1.00)	0.588 (1.00)	0.351 (1.00)	0.305 (1.00)	0.742 (1.00)	1 (1.00)	1 (1.00)	0.565 (1.00)	1 (1.00)	0.791 (1.00)
PCDH10	17 (8%)	207	0.209 (1.00)	0.976 (1.00)	0.283 (1.00)	0.206 (1.00)	0.357 (1.00)	0.518 (1.00)	0.563 (1.00)	0.518 (1.00)	0.619 (1.00)	0.761 (1.00)	0.216 (1.00)
MCF2	12 (5%)	212	0.479 (1.00)	0.476 (1.00)	1 (1.00)	0.557 (1.00)	0.27 (1.00)	0.0208 (1.00)	0.599 (1.00)	1 (1.00)	0.702 (1.00)	1 (1.00)	0.864 (1.00)
NCKAP5	15 (7%)	209	0.796 (1.00)	0.637 (1.00)	0.158 (1.00)	1 (1.00)	0.437 (1.00)	0.213 (1.00)	0.704 (1.00)	0.461 (1.00)	0.739 (1.00)	0.726 (1.00)	0.796 (1.00)
RIMS2	13 (6%)	211	0.208 (1.00)	0.763 (1.00)	0.0705 (1.00)	0.777 (1.00)	0.1 (1.00)	0.214 (1.00)	0.926 (1.00)	0.697 (1.00)	0.756 (1.00)	0.705 (1.00)	0.583 (1.00)
ATP7A	6 (3%)	218		0.178 (1.00)	0.669 (1.00)	0.429 (1.00)	0.426 (1.00)	0.0227 (1.00)	0.29 (1.00)	0.222 (1.00)	0.127 (1.00)	0.593 (1.00)	0.917 (1.00)
TPTE	12 (5%)	212	0.657 (1.00)	0.562 (1.00)	0.111 (1.00)	0.557 (1.00)	0.0724 (1.00)	0.444 (1.00)	0.411 (1.00)	1 (1.00)	0.944 (1.00)	1 (1.00)	0.803 (1.00)
OR8B4	6 (3%)	218	0.705 (1.00)	0.839 (1.00)	0.327 (1.00)	0.429 (1.00)	0.426 (1.00)	0.689 (1.00)	0.535 (1.00)	0.609 (1.00)	0.666 (1.00)	1 (1.00)	0.061 (1.00)
SFMBT2	13 (6%)	211	0.398 (1.00)	0.657 (1.00)	0.0705 (1.00)	0.575 (1.00)	0.207 (1.00)	0.258 (1.00)	0.619 (1.00)	0.697 (1.00)	0.862 (1.00)	0.705 (1.00)	0.538 (1.00)
FLRT2	13 (6%)	211	0.744 (1.00)	0.697 (1.00)	0.353 (1.00)	0.395 (1.00)	0.797 (1.00)	0.0547 (1.00)	0.53 (1.00)	0.269 (1.00)	0.47 (1.00)	0.484 (1.00)	0.0458 (1.00)
BCHE	9 (4%)	215	0.427 (1.00)	0.576 (1.00)	0.725 (1.00)	0.175 (1.00)	1 (1.00)	0.228 (1.00)	0.315 (1.00)	1 (1.00)	0.733 (1.00)	1 (1.00)	0.718 (1.00)
C8B	10 (4%)	214	0.263 (1.00)	0.122 (1.00)	0.29 (1.00)	0.2 (1.00)	0.182 (1.00)	0.414 (1.00)	1 (1.00)	0.652 (1.00)	0.526 (1.00)	1 (1.00)	0.839 (1.00)
FREM2	23 (10%)	201	0.612 (1.00)	0.139 (1.00)	0.473 (1.00)	0.0138 (1.00)	0.701 (1.00)	0.11 (1.00)	0.715 (1.00)	0.387 (1.00)	0.581 (1.00)	0.509 (1.00)	0.0471 (1.00)
GABRG1	8 (4%)	216	0.514 (1.00)	0.0941 (1.00)	1 (1.00)	0.0293 (1.00)	0.562 (1.00)	0.303 (1.00)	1 (1.00)	0.619 (1.00)	0.708 (1.00)	0.643 (1.00)	0.221 (1.00)
SAT1	4 (2%)	220		0.211 (1.00)	0.315 (1.00)	0.0505 (1.00)	0.397 (1.00)	1 (1.00)	1 (1.00)	1 (1.00)	0.897 (1.00)	1 (1.00)	0.851 (1.00)
CHD4	17 (8%)	207	0.715 (1.00)	0.514 (1.00)	1 (1.00)	1 (1.00)	0.467 (1.00)	0.386 (1.00)	0.0527 (1.00)	0.728 (1.00)	0.354 (1.00)	0.761 (1.00)	0.00928 (1.00)
PCDHB2	14 (6%)	210	0.321 (1.00)	0.876 (1.00)	0.0692 (1.00)	0.584 (1.00)	0.309 (1.00)	0.669 (1.00)	0.684 (1.00)	1 (1.00)	0.975 (1.00)	1 (1.00)	0.314 (1.00)
TMTC1	13 (6%)	211	0.857 (1.00)	0.599 (1.00)	1 (1.00)	1 (1.00)	0.366 (1.00)	0.673 (1.00)	0.926 (1.00)	0.267 (1.00)	0.622 (1.00)	0.668 (1.00)	0.818 (1.00)
SH3GL3	8 (4%)	216	0.468 (1.00)	0.196 (1.00)	0.703 (1.00)	0.484 (1.00)	0.824 (1.00)	0.223 (1.00)	0.418 (1.00)	0.61 (1.00)	0.789 (1.00)	0.0986 (1.00)	0.0133 (1.00)
CACNG3	8 (4%)	216	0.468 (1.00)	0.145 (1.00)	0.703 (1.00)	0.484 (1.00)	0.321 (1.00)	0.335 (1.00)	0.89 (1.00)	0.619 (1.00)	0.849 (1.00)	0.643 (1.00)	0.87 (1.00)
POTEB	8 (4%)	216	0.634 (1.00)	0.529 (1.00)	0.703 (1.00)	1 (1.00)	0.409 (1.00)	0.223 (1.00)	0.325 (1.00)	0.61 (1.00)	0.43 (1.00)	0.637 (1.00)	0.000806 (1.00)
GRIN2A	19 (8%)	205	0.457 (1.00)	0.528 (1.00)	0.441 (1.00)	0.348 (1.00)	0.943 (1.00)	0.446 (1.00)	0.073 (1.00)	1 (1.00)	0.697 (1.00)	1 (1.00)	0.158 (1.00)
NRXN3	17 (8%)	207	0.879 (1.00)	0.738 (1.00)	0.102 (1.00)	0.622 (1.00)	0.0804 (1.00)	0.51 (1.00)	0.336 (1.00)	0.199 (1.00)	0.448 (1.00)	0.761 (1.00)	0.0203 (1.00)
OR7C1	6 (3%)	218		0.12 (1.00)	0.327 (1.00)	0.429 (1.00)	0.196 (1.00)	0.315 (1.00)	1 (1.00)	1 (1.00)	0.838 (1.00)	1 (1.00)	0.64 (1.00)
SPATA8	3 (1%)	221		0.706 (1.00)	0.0279 (1.00)	0.107 (1.00)	0.0616 (1.00)	1 (1.00)	0.778 (1.00)	1 (1.00)	0.863 (1.00)	1 (1.00)	0.331 (1.00)
OR2M2	6 (3%)	218	0.106 (1.00)	0.535 (1.00)	0.327 (1.00)	0.106 (1.00)	0.0347 (1.00)	0.0365 (1.00)	1 (1.00)	0.166 (1.00)	0.212 (1.00)	0.179 (1.00)	0.189 (1.00)
SORCS1	15 (7%)	209	0.0276 (1.00)	0.116 (1.00)	1 (1.00)	1 (1.00)	0.871 (1.00)	0.0128 (1.00)	0.429 (1.00)	1 (1.00)	0.374 (1.00)	0.503 (1.00)	0.00977 (1.00)
IL18R1	10 (4%)	214	0.443 (1.00)	0.455 (1.00)	0.727 (1.00)	1 (1.00)	0.282 (1.00)	0.0217 (1.00)	0.604 (1.00)	0.393 (1.00)	0.229 (1.00)	0.653 (1.00)	0.766 (1.00)
C16ORF45	3 (1%)	221		0.795 (1.00)	1 (1.00)	1 (1.00)	1 (1.00)	0.217 (1.00)	0.132 (1.00)	0.0161 (1.00)	0.213 (1.00)		0.827 (1.00)
INHBA	9 (4%)	215	0.911 (1.00)	0.204 (1.00)	0.725 (1.00)	0.503 (1.00)	0.00297 (1.00)	0.137 (1.00)	1 (1.00)	0.392 (1.00)	0.565 (1.00)	0.653 (1.00)	0.945 (1.00)
MIER3	11 (5%)	213	0.442 (1.00)	0.905 (1.00)	0.179 (1.00)	0.761 (1.00)	0.504 (1.00)	0.321 (1.00)	0.214 (1.00)	0.393 (1.00)	0.5 (1.00)	0.668 (1.00)	0.00059 (1.00)
ZNF585A	10 (4%)	214	0.479 (1.00)	0.504 (1.00)	1 (1.00)	1 (1.00)	0.3 (1.00)	0.17 (1.00)	0.479 (1.00)	0.392 (1.00)	0.702 (1.00)	0.643 (1.00)	0.0925 (1.00)
FSTL5	11 (5%)	213	0.383 (1.00)	0.437 (1.00)	1 (1.00)	0.123 (1.00)	0.823 (1.00)	0.0547 (1.00)	0.694 (1.00)	1 (1.00)	0.79 (1.00)	1 (1.00)	0.281 (1.00)
DOK5	6 (3%)	218	0.704 (1.00)	0.0225 (1.00)	1 (1.00)	0.106 (1.00)	0.0676 (1.00)	1 (1.00)	0.445 (1.00)	1 (1.00)	0.89 (1.00)	0.527 (1.00)	0.96 (1.00)
ZNF99	11 (5%)	213	0.112 (1.00)	0.731 (1.00)	1 (1.00)	1 (1.00)	0.296 (1.00)	0.248 (1.00)	0.694 (1.00)	0.393 (1.00)	0.726 (1.00)	0.668 (1.00)	0.00681 (1.00)
GML	6 (3%)	218	0.164 (1.00)	0.85 (1.00)	0.669 (1.00)	0.215 (1.00)	0.763 (1.00)	0.826 (1.00)	0.733 (1.00)	0.546 (1.00)	0.741 (1.00)	0.527 (1.00)	0.36 (1.00)
PPP2R2B	3 (1%)	221		0.532 (1.00)	0.223 (1.00)	0.107 (1.00)	0.105 (1.00)	0.00532 (1.00)	0.778 (1.00)	1 (1.00)	0.367 (1.00)	1 (1.00)
LRTM1	7 (3%)	217	0.603 (1.00)	0.713 (1.00)	1 (1.00)	0.449 (1.00)	0.896 (1.00)	0.167 (1.00)	1 (1.00)	0.61 (1.00)	0.906 (1.00)	0.637 (1.00)	0.14 (1.00)
OR10G9	6 (3%)	218	0.295 (1.00)	0.944 (1.00)	0.669 (1.00)	0.106 (1.00)	0.00497 (1.00)	0.194 (1.00)	0.629 (1.00)	0.609 (1.00)	0.746 (1.00)	1 (1.00)	0.061 (1.00)
ERBB3	14 (6%)	210	0.354 (1.00)	0.662 (1.00)	0.559 (1.00)	1 (1.00)	0.486 (1.00)	0.669 (1.00)	0.862 (1.00)	0.717 (1.00)	0.78 (1.00)	1 (1.00)	0.626 (1.00)
PSG8	8 (4%)	216	0.176 (1.00)	0.991 (1.00)	0.11 (1.00)	1 (1.00)	0.21 (1.00)	0.223 (1.00)	0.89 (1.00)	1 (1.00)	0.471 (1.00)	0.0345 (1.00)	0.195 (1.00)
SYT16	9 (4%)	215	0.458 (1.00)	0.202 (1.00)	1 (1.00)	1 (1.00)	0.535 (1.00)	0.281 (1.00)	0.282 (1.00)	0.392 (1.00)	0.269 (1.00)	0.653 (1.00)	0.244 (1.00)
LPHN3	13 (6%)	211	0.401 (1.00)	0.197 (1.00)	0.225 (1.00)	1 (1.00)	0.397 (1.00)	0.00229 (1.00)	0.281 (1.00)	1 (1.00)	0.194 (1.00)	0.175 (1.00)	0.351 (1.00)
LRP2	35 (16%)	189	0.601 (1.00)	0.494 (1.00)	0.164 (1.00)	0.463 (1.00)	0.0823 (1.00)	0.309 (1.00)	0.934 (1.00)	0.308 (1.00)	0.561 (1.00)	0.627 (1.00)	0.943 (1.00)
SOHLH2	9 (4%)	215	0.122 (1.00)	0.35 (1.00)	0.282 (1.00)	1 (1.00)	0.717 (1.00)	0.0621 (1.00)	0.562 (1.00)	1 (1.00)	0.918 (1.00)	1 (1.00)	0.825 (1.00)
ZIM3	9 (4%)	215	0.585 (1.00)	0.16 (1.00)	0.137 (1.00)	0.74 (1.00)	0.193 (1.00)	0.107 (1.00)	0.253 (1.00)	0.619 (1.00)	0.789 (1.00)	0.0879 (1.00)	0.00743 (1.00)
DSEL	14 (6%)	210	0.914 (1.00)	0.846 (1.00)	0.236 (1.00)	0.271 (1.00)	0.179 (1.00)	0.0727 (1.00)	0.121 (1.00)	0.717 (1.00)	0.647 (1.00)	1 (1.00)	0.000545 (1.00)
MDGA2	10 (4%)	214	0.733 (1.00)	0.366 (1.00)	0.727 (1.00)	1 (1.00)	0.282 (1.00)	0.414 (1.00)	0.902 (1.00)	1 (1.00)	0.814 (1.00)	1 (1.00)	0.237 (1.00)
RBMS3	6 (3%)	218	0.425 (1.00)	0.682 (1.00)	0.669 (1.00)	0.429 (1.00)	0.763 (1.00)	0.17 (1.00)	1 (1.00)	1 (1.00)	0.89 (1.00)	0.527 (1.00)	0.543 (1.00)
TMBIM4	4 (2%)	220		0.426 (1.00)	1 (1.00)	0.623 (1.00)	1 (1.00)	0.14 (1.00)	0.647 (1.00)	0.376 (1.00)	0.489 (1.00)	0.36 (1.00)	0.131 (1.00)
FAM135B	15 (7%)	209	0.877 (1.00)	0.676 (1.00)	0.562 (1.00)	1 (1.00)	0.613 (1.00)	0.533 (1.00)	0.704 (1.00)	1 (1.00)	0.573 (1.00)	1 (1.00)	0.675 (1.00)
CYP7B1	7 (3%)	217	0.935 (1.00)	0.797 (1.00)	1 (1.00)	0.449 (1.00)	0.106 (1.00)	0.0196 (1.00)	1 (1.00)	1 (1.00)	0.273 (1.00)	1 (1.00)	0.957 (1.00)
SLC16A7	9 (4%)	215	0.246 (1.00)	0.586 (1.00)	0.282 (1.00)	0.74 (1.00)	0.166 (1.00)	0.137 (1.00)	0.803 (1.00)	0.392 (1.00)	0.839 (1.00)	0.653 (1.00)	0.0533 (1.00)

'PIK3CA MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.26e-05 (t-test), Q value = 0.028

Table S1. Gene #3: 'PIK3CA MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
PIK3CA MUTATED	33	0.5 (1.4)
PIK3CA WILD-TYPE	189	2.6 (5.1)

Figure S1. Get High-res Image Gene #3: 'PIK3CA MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'BRAF MUTATION STATUS' versus 'HISTOLOGICAL.TYPE'

P value = 2e-06 (Fisher's exact test), Q value = 0.0045

Table S2. Gene #5: 'BRAF MUTATION STATUS' versus Clinical Feature #5: 'HISTOLOGICAL.TYPE'

nPatients	COLON ADENOCARCINOMA	COLON MUCINOUS ADENOCARCINOMA	RECTAL ADENOCARCINOMA	RECTAL MUCINOUS ADENOCARCINOMA
ALL	128	24	57	8
BRAF MUTATED	9	11	1	1
BRAF WILD-TYPE	119	13	56	7

Figure S2. Get High-res Image Gene #5: 'BRAF MUTATION STATUS' versus Clinical Feature #5: 'HISTOLOGICAL.TYPE'

'KRTAP5-5 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.21e-11 (t-test), Q value = 7.3e-08

Table S3. Gene #14: 'KRTAP5-5 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
KRTAP5-5 MUTATED	4	0.0 (0.0)
KRTAP5-5 WILD-TYPE	218	2.3 (4.8)

Figure S3. Get High-res Image Gene #14: 'KRTAP5-5 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'NRXN1 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 2.98e-05 (t-test), Q value = 0.067

Table S4. Gene #22: 'NRXN1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
NRXN1 MUTATED	20	0.5 (1.3)
NRXN1 WILD-TYPE	202	2.4 (5.0)

Figure S4. Get High-res Image Gene #22: 'NRXN1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'CNTN6 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 9.11e-05 (t-test), Q value = 0.2

Table S5. Gene #32: 'CNTN6 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
CNTN6 MUTATED	17	0.5 (1.3)
CNTN6 WILD-TYPE	205	2.4 (5.0)

Figure S5. Get High-res Image Gene #32: 'CNTN6 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'CDH2 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 8.45e-06 (t-test), Q value = 0.019

Table S6. Gene #35: 'CDH2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
CDH2 MUTATED	16	0.4 (1.0)
CDH2 WILD-TYPE	206	2.4 (5.0)

Figure S6. Get High-res Image Gene #35: 'CDH2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'CNBD1 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 5.88e-05 (t-test), Q value = 0.13

Table S7. Gene #36: 'CNBD1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
CNBD1 MUTATED	7	0.3 (0.8)
CNBD1 WILD-TYPE	215	2.3 (4.9)

Figure S7. Get High-res Image Gene #36: 'CNBD1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'ERBB4 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 5.02e-08 (t-test), Q value = 0.00011

Table S8. Gene #39: 'ERBB4 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
ERBB4 MUTATED	18	0.2 (0.7)
ERBB4 WILD-TYPE	204	2.4 (5.0)

Figure S8. Get High-res Image Gene #39: 'ERBB4 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'ACVR2A MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.99e-08 (t-test), Q value = 9e-05

Table S9. Gene #48: 'ACVR2A MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
ACVR2A MUTATED	9	0.2 (0.4)
ACVR2A WILD-TYPE	213	2.3 (4.9)

Figure S9. Get High-res Image Gene #48: 'ACVR2A MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'CDH9 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.74e-10 (t-test), Q value = 3.9e-07

Table S10. Gene #70: 'CDH9 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
CDH9 MUTATED	15	0.1 (0.3)
CDH9 WILD-TYPE	207	2.4 (4.9)

Figure S10. Get High-res Image Gene #70: 'CDH9 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'GRIK3 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 0.000109 (t-test), Q value = 0.25

Table S11. Gene #71: 'GRIK3 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
GRIK3 MUTATED	17	0.5 (1.2)
GRIK3 WILD-TYPE	205	2.4 (5.0)

Figure S11. Get High-res Image Gene #71: 'GRIK3 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'GGT1 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.24e-11 (t-test), Q value = 7.4e-08

Table S12. Gene #88: 'GGT1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
GGT1 MUTATED	3	0.0 (0.0)
GGT1 WILD-TYPE	219	2.3 (4.8)

Figure S12. Get High-res Image Gene #88: 'GGT1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'PCDHA10 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.1e-07 (t-test), Q value = 7e-04

Table S13. Gene #91: 'PCDHA10 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
PCDHA10 MUTATED	10	0.2 (0.6)
PCDHA10 WILD-TYPE	212	2.3 (4.9)

Figure S13. Get High-res Image Gene #91: 'PCDHA10 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'FAM5C MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 2.7e-10 (t-test), Q value = 6.1e-07

Table S14. Gene #94: 'FAM5C MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
FAM5C MUTATED	13	0.1 (0.3)
FAM5C WILD-TYPE	209	2.4 (4.9)

Figure S14. Get High-res Image Gene #94: 'FAM5C MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'IFT80 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 8.54e-10 (t-test), Q value = 1.9e-06

Table S15. Gene #99: 'IFT80 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
IFT80 MUTATED	10	0.1 (0.3)
IFT80 WILD-TYPE	212	2.4 (4.9)

Figure S15. Get High-res Image Gene #99: 'IFT80 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'DACH2 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 5.01e-05 (t-test), Q value = 0.11

Table S16. Gene #118: 'DACH2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
DACH2 MUTATED	12	0.5 (0.9)
DACH2 WILD-TYPE	210	2.4 (4.9)

Figure S16. Get High-res Image Gene #118: 'DACH2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'PCDH11X MUTATION STATUS' versus 'Time to Death'

P value = 6.96e-05 (logrank test), Q value = 0.16

Table S17. Gene #120: 'PCDH11X MUTATION STATUS' versus Clinical Feature #1: 'Time to Death'

	nPatients	nDeath	Duration Range (Median), Month
ALL	120	14	0.9 - 72.1 (10.9)
PCDH11X MUTATED	11	4	1.0 - 21.0 (1.0)
PCDH11X WILD-TYPE	109	10	0.9 - 72.1 (12.0)

Figure S17. Get High-res Image Gene #120: 'PCDH11X MUTATION STATUS' versus Clinical Feature #1: 'Time to Death'

'BTNL8 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.24e-11 (t-test), Q value = 7.4e-08

Table S18. Gene #126: 'BTNL8 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
BTNL8 MUTATED	3	0.0 (0.0)
BTNL8 WILD-TYPE	219	2.3 (4.8)

Figure S18. Get High-res Image Gene #126: 'BTNL8 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'NXF5 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.11e-11 (t-test), Q value = 7.1e-08

Table S19. Gene #129: 'NXF5 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
NXF5 MUTATED	7	0.0 (0.0)
NXF5 WILD-TYPE	215	2.3 (4.9)

Figure S19. Get High-res Image Gene #129: 'NXF5 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'SPATA17 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.4e-06 (t-test), Q value = 0.0032

Table S20. Gene #134: 'SPATA17 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
SPATA17 MUTATED	9	0.2 (0.7)
SPATA17 WILD-TYPE	213	2.3 (4.9)

Figure S20. Get High-res Image Gene #134: 'SPATA17 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'ZCWPW2 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.11e-11 (t-test), Q value = 7.1e-08

Table S21. Gene #143: 'ZCWPW2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
ZCWPW2 MUTATED	7	0.0 (0.0)
ZCWPW2 WILD-TYPE	215	2.3 (4.9)

Figure S21. Get High-res Image Gene #143: 'ZCWPW2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'CDH12 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.32e-08 (t-test), Q value = 3e-05

Table S22. Gene #151: 'CDH12 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
CDH12 MUTATED	13	0.2 (0.6)
CDH12 WILD-TYPE	209	2.4 (4.9)

Figure S22. Get High-res Image Gene #151: 'CDH12 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'ZNF167 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.5e-08 (t-test), Q value = 3.4e-05

Table S23. Gene #163: 'ZNF167 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
ZNF167 MUTATED	7	0.1 (0.4)
ZNF167 WILD-TYPE	215	2.3 (4.9)

Figure S23. Get High-res Image Gene #163: 'ZNF167 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'ZFPM2 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.03e-07 (t-test), Q value = 0.00023

Table S24. Gene #170: 'ZFPM2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
ZFPM2 MUTATED	6	0.2 (0.4)
ZFPM2 WILD-TYPE	216	2.3 (4.9)

Figure S24. Get High-res Image Gene #170: 'ZFPM2 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'DPY19L1 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.18e-11 (t-test), Q value = 7.2e-08

Table S25. Gene #180: 'DPY19L1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
DPY19L1 MUTATED	5	0.0 (0.0)
DPY19L1 WILD-TYPE	217	2.3 (4.8)

Figure S25. Get High-res Image Gene #180: 'DPY19L1 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'TMPRSS13 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.21e-11 (t-test), Q value = 7.3e-08

Table S26. Gene #185: 'TMPRSS13 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
TMPRSS13 MUTATED	4	0.0 (0.0)
TMPRSS13 WILD-TYPE	218	2.3 (4.8)

Figure S26. Get High-res Image Gene #185: 'TMPRSS13 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'B2M MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.67e-06 (t-test), Q value = 0.0038

Table S27. Gene #190: 'B2M MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
B2M MUTATED	5	0.2 (0.4)
B2M WILD-TYPE	217	2.3 (4.9)

Figure S27. Get High-res Image Gene #190: 'B2M MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'RNASE11 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 3.18e-11 (t-test), Q value = 7.2e-08

Table S28. Gene #202: 'RNASE11 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
RNASE11 MUTATED	5	0.0 (0.0)
RNASE11 WILD-TYPE	217	2.3 (4.8)

Figure S28. Get High-res Image Gene #202: 'RNASE11 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

'COL6A6 MUTATION STATUS' versus 'NUMBER.OF.LYMPH.NODES'

P value = 1.98e-07 (t-test), Q value = 0.00045

Table S29. Gene #205: 'COL6A6 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

	nPatients	Mean (Std.Dev)
ALL	222	2.3 (4.8)
COL6A6 MUTATED	16	0.2 (0.8)
COL6A6 WILD-TYPE	206	2.4 (5.0)

Figure S29. Get High-res Image Gene #205: 'COL6A6 MUTATION STATUS' versus Clinical Feature #11: 'NUMBER.OF.LYMPH.NODES'

Methods & Data

Input

Mutation data file = COADREAD-TP.mutsig.cluster.txt
Clinical data file = COADREAD-TP.clin.merged.picked.txt
Number of patients = 224
Number of significantly mutated genes = 209
Number of selected clinical features = 11
Exclude genes that fewer than K tumors have mutations, K = 3

Survival analysis

For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R

Student's t-test analysis

For continuous numerical clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the clinical values between tumors with and without gene mutations using 't.test' function in R

Fisher's exact test

For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R

Q value calculation

For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.

Download Results

This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.

References

[1] Bland and Altman, Statistics notes: The logrank test, BMJ 328(7447):1073 (2004)

[2] Lehmann and Romano, Testing Statistical Hypotheses (3E ed.), New York: Springer. ISBN 0387988645 (2005)

[3] Fisher, R.A., On the interpretation of chi-square from contingency tables, and the calculation of P, Journal of the Royal Statistical Society 85(1):87-94 (1922)

[4] Benjamini and Hochberg, Controlling the false discovery rate: a practical and powerful approach to multiple testing, Journal of the Royal Statistical Society Series B 59:289-300 (1995)

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