Thyroid Adenocarcinoma: Mutation Analysis (MutSig v1.5)
(primary solid tumor cohort)
Maintained by Dan DiCara (Broad Institute)
Overview
Introduction

This report serves to describe the mutational landscape and properties of a given individual set, as well as rank genes and genesets according to mutational significance. MutSig v1.5 was used to generate the results found in this report.

- Working with individual set: THCA-TP

- Number of patients in set: 323

Input

The input for this pipeline is a set of individuals with the following files associated for each:

1. An annotated .maf file describing the mutations called for the respective individual, and their properties.

2. A .wig file that contains information about the coverage of the sample.

Summary

- MAF used for this analysis:THCA-TP.final_analysis_set.maf

- Significantly mutated genes (q ≤ 0.1): 26

- Mutations seen in COSMIC: 249

- Significantly mutated genes in COSMIC territory: 11

- Genes with clustered mutations (≤ 3 aa apart): 47

- Significantly mutated genesets: 72

- Significantly mutated genesets: (excluding sig. mutated genes):0

Mutation Preprocessing

- Read 323 MAFs of type "Broad"

- Total number of mutations in input MAFs: 11952

- After removing 99 mutations outside chr1-24: 11853

- After removing 1530 blacklisted mutations: 10323

- After removing 3226 noncoding mutations: 7097

Mutation Filtering

- Number of mutations before filtering: 7097

- After removing 289 mutations outside gene set: 6808

- After removing 2 mutations outside category set: 6806

Results
Breakdown of Mutations by Type

Table 1. Get Full Table Table representing breakdown of mutations by type.

type | count
--- | ---
Frame_Shift_Del | 375
Frame_Shift_Ins | 112
In_Frame_Del | 254
In_Frame_Ins | 18
Missense_Mutation | 4143
Nonsense_Mutation | 194
Nonstop_Mutation | 4
Silent | 1573
Splice_Site | 125
Translation_Start_Site | 8
Total | 6806

Breakdown of Mutation Rates by Category Type

Table 2. Get Full Table A breakdown of mutation rates per category discovered for this individual set.

category | n | N | rate | rate_per_mb | relative_rate | exp_ns_s_ratio
--- | --- | --- | --- | --- | --- | ---
*CpG->T | 782 | 526711238 | 1.5e-06 | 1.5 | 2.7 | 2.1
*Cp(A/C/T)->T | 981 | 4313476957 | 2.3e-07 | 0.23 | 0.41 | 1.7
A->G | 826 | 4649186676 | 1.8e-07 | 0.18 | 0.32 | 2.3
transver | 1558 | 9489374871 | 1.6e-07 | 0.16 | 0.3 | 5
indel+null | 1084 | 9489374871 | 1.1e-07 | 0.11 | 0.21 | NaN
double_null | 2 | 9489374871 | 2.1e-10 | 0.00021 | 0.00038 | NaN
Total | 5233 | 9489374871 | 5.5e-07 | 0.55 | 1 | 3.5

Target Coverage for Each Individual

The x axis represents the samples. The y axis represents the exons, one row per exon, and they are sorted by average coverage across samples. For exons with exactly the same average coverage, they are sorted next by the %GC of the exon. (The secondary sort is especially useful for the zero-coverage exons at the bottom).

Figure 1.

Distribution of Mutation Counts, Coverage, and Mutation Rates Across Samples

Figure 2. Patients counts and rates file used to generate this plot: THCA-TP.patients.counts_and_rates.txt

CoMut Plot

Figure 3. Get High-res Image The matrix in the center of the figure represents individual mutations in patient samples, color-coded by type of mutation, for the significantly mutated genes. The rate of synonymous and non-synonymous mutations is displayed at the top of the matrix. The barplot on the left of the matrix shows the number of mutations in each gene. The percentages represent the fraction of tumors with at least one mutation in the specified gene. The barplot to the right of the matrix displays the q-values for the most significantly mutated genes. The purple boxplots below the matrix (only displayed if required columns are present in the provided MAF) represent the distributions of allelic fractions observed in each sample. The plot at the bottom represents the base substitution distribution of individual samples, using the same categories that were used to calculate significance.

Significantly Mutated Genes

Column Descriptions:

- N = number of sequenced bases in this gene across the individual set

- n = number of (nonsilent) mutations in this gene across the individual set

- npat = number of patients (individuals) with at least one nonsilent mutation

- nsite = number of unique sites having a non-silent mutation

- nsil = number of silent mutations in this gene across the individual set

- n1 = number of nonsilent mutations of type: *CpG->T

- n2 = number of nonsilent mutations of type: *Cp(A/C/T)->T

- n3 = number of nonsilent mutations of type: A->G

- n4 = number of nonsilent mutations of type: transver

- n5 = number of nonsilent mutations of type: indel+null

- n6 = number of nonsilent mutations of type: double_null

- p_ns_s = p-value for the observed nonsilent/silent ratio being elevated in this gene

- p = p-value (overall)

- q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)

Table 3. Get Full Table A Ranked List of Significantly Mutated Genes. Number of significant genes found: 26. Number of genes displayed: 35. Click on a gene name to display its stick figure depicting the distribution of mutations and mutation types across the chosen gene (this feature may not be available for all significant genes).

rank | gene | description | N | n | npat | nsite | nsil | n1 | n2 | n3 | n4 | n5 | n6 | p_ns_s | p | q
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
1 | BRAF | v-raf murine sarcoma viral oncogene homolog B1 | 718092 | 183 | 183 | 2 | 1 | 0 | 0 | 1 | 182 | 0 | 0 | 5e-14 | <1.00e-15 | <1.81e-11
2 | NRAS | neuroblastoma RAS viral (v-ras) oncogene homolog | 189273 | 26 | 26 | 2 | 0 | 0 | 0 | 20 | 6 | 0 | 0 | 0.00016 | 4.33e-15 | 3.92e-11
3 | HRAS | v-Ha-ras Harvey rat sarcoma viral oncogene homolog | 209181 | 12 | 12 | 2 | 0 | 0 | 0 | 9 | 3 | 0 | 0 | 0.13 | 1.95e-14 | 1.18e-10
4 | EIF1AX | eukaryotic translation initiation factor 1A, X-linked | 142447 | 6 | 5 | 5 | 0 | 0 | 4 | 0 | 1 | 1 | 0 | 0.24 | 7.17e-10 | 3.07e-06
5 | EMG1 | EMG1 nucleolar protein homolog (S. cerevisiae) | 220090 | 6 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 1 | 8.48e-10 | 3.07e-06
6 | RPTN | repetin | 763230 | 8 | 8 | 6 | 0 | 0 | 0 | 1 | 0 | 7 | 0 | 0.65 | 5.57e-09 | 1.68e-05
7 | TG | thyroglobulin | 2717568 | 16 | 16 | 16 | 3 | 1 | 0 | 1 | 4 | 10 | 0 | 0.82 | 1.13e-08 | 2.92e-05
8 | PRB2 | proline-rich protein BstNI subfamily 2 | 405776 | 6 | 6 | 4 | 1 | 0 | 1 | 0 | 2 | 3 | 0 | 0.65 | 5.76e-08 | 0.000130
9 | MUC7 | mucin 7, secreted | 368847 | 5 | 5 | 5 | 1 | 0 | 2 | 2 | 0 | 1 | 0 | 0.41 | 4.15e-07 | 0.000835
10 | PTTG1IP | pituitary tumor-transforming 1 interacting protein | 144700 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 1.48e-06 | 0.00268
11 | ZNF845 | zinc finger protein 845 | 928346 | 6 | 6 | 3 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0.09 | 3.81e-06 | 0.00627
12 | PPM1D | protein phosphatase 1D magnesium-dependent, delta isoform | 502843 | 5 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0.7 | 4.52e-06 | 0.00682
13 | ZNF799 | zinc finger protein 799 | 607484 | 5 | 5 | 2 | 1 | 0 | 0 | 5 | 0 | 0 | 0 | 0.26 | 7.23e-06 | 0.0101
14 | IL32 | interleukin 32 | 168065 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1.65e-05 | 0.0213
15 | CCDC15 | coiled-coil domain containing 15 | 671425 | 5 | 5 | 1 | 1 | 0 | 0 | 0 | 5 | 0 | 0 | 0.72 | 2.31e-05 | 0.0279
16 | SLA | Src-like-adaptor | 265974 | 3 | 3 | 3 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0.63 | 4.85e-05 | 0.0549
17 | COL5A3 | collagen, type V, alpha 3 | 1579087 | 6 | 6 | 6 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0.29 | 5.94e-05 | 0.0632
18 | TMCO2 | transmembrane and coiled-coil domains 2 | 179663 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 6.72e-05 | 0.0675
19 | SLC25A45 | solute carrier family 25, member 45 | 287759 | 3 | 3 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0.33 | 8.54e-05 | 0.0813
20 | ZFHX3 | zinc finger homeobox 3 | 3589602 | 10 | 10 | 9 | 2 | 0 | 1 | 2 | 3 | 4 | 0 | 0.64 | 9.45e-05 | 0.0855
21 | ZNF878 | zinc finger protein 878 | 523994 | 4 | 4 | 2 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0.54 | 0.000105 | 0.0903
22 | KRTAP4-4 | keratin associated protein 4-4 | 163115 | 2 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0.000120 | 0.0934
23 | DNMT3A | DNA (cytosine-5-)-methyltransferase 3 alpha | 868356 | 5 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0.21 | 0.000126 | 0.0934
24 | KRAS | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | 224332 | 3 | 3 | 3 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0.48 | 0.000128 | 0.0934
25 | MSI1 | musashi homolog 1 (Drosophila) | 257818 | 3 | 3 | 3 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0.5 | 0.000131 | 0.0934
26 | FAM155A | family with sequence similarity 155, member A | 434292 | 4 | 4 | 3 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 0.76 | 0.000134 | 0.0934
27 | PAWR | PRKC, apoptosis, WT1, regulator | 176235 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0.62 | 0.000152 | 0.102
28 | MED31 | mediator complex subunit 31 | 131968 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0.96 | 0.000178 | 0.115
29 | PPTC7 | PTC7 protein phosphatase homolog (S. cerevisiae) | 230856 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0.000200 | 0.123
30 | C11orf87 | chromosome 11 open reading frame 87 | 188643 | 3 | 3 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0.3 | 0.000213 | 0.123
31 | ZNF780A | zinc finger protein 780A | 622994 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0.79 | 0.000223 | 0.123
32 | OR13F1 | olfactory receptor, family 13, subfamily F, member 1 | 310712 | 3 | 3 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0.27 | 0.000224 | 0.123
33 | MAP3K3 | mitogen-activated protein kinase kinase kinase 3 | 653710 | 4 | 4 | 4 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0.28 | 0.000227 | 0.123
34 | ANKRD30A | ankyrin repeat domain 30A | 1168065 | 5 | 5 | 4 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0.5 | 0.000232 | 0.123
35 | DBR1 | debranching enzyme homolog 1 (S. cerevisiae) | 531244 | 3 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0.63 | 0.000291 | 0.151

BRAF

Figure S1. This figure depicts the distribution of mutations and mutation types across the BRAF significant gene.

NRAS

Figure S2. This figure depicts the distribution of mutations and mutation types across the NRAS significant gene.

HRAS

Figure S3. This figure depicts the distribution of mutations and mutation types across the HRAS significant gene.

EIF1AX

Figure S4. This figure depicts the distribution of mutations and mutation types across the EIF1AX significant gene.

EMG1

Figure S5. This figure depicts the distribution of mutations and mutation types across the EMG1 significant gene.

RPTN

Figure S6. This figure depicts the distribution of mutations and mutation types across the RPTN significant gene.

TG

Figure S7. This figure depicts the distribution of mutations and mutation types across the TG significant gene.

MUC7

Figure S8. This figure depicts the distribution of mutations and mutation types across the MUC7 significant gene.

PTTG1IP

Figure S9. This figure depicts the distribution of mutations and mutation types across the PTTG1IP significant gene.

ZNF845

Figure S10. This figure depicts the distribution of mutations and mutation types across the ZNF845 significant gene.

PPM1D

Figure S11. This figure depicts the distribution of mutations and mutation types across the PPM1D significant gene.

ZNF799

Figure S12. This figure depicts the distribution of mutations and mutation types across the ZNF799 significant gene.

IL32

Figure S13. This figure depicts the distribution of mutations and mutation types across the IL32 significant gene.

CCDC15

Figure S14. This figure depicts the distribution of mutations and mutation types across the CCDC15 significant gene.

SLA

Figure S15. This figure depicts the distribution of mutations and mutation types across the SLA significant gene.

COL5A3

Figure S16. This figure depicts the distribution of mutations and mutation types across the COL5A3 significant gene.

TMCO2

Figure S17. This figure depicts the distribution of mutations and mutation types across the TMCO2 significant gene.

SLC25A45

Figure S18. This figure depicts the distribution of mutations and mutation types across the SLC25A45 significant gene.

ZFHX3

Figure S19. This figure depicts the distribution of mutations and mutation types across the ZFHX3 significant gene.

ZNF878

Figure S20. This figure depicts the distribution of mutations and mutation types across the ZNF878 significant gene.

KRTAP4-4

Figure S21. This figure depicts the distribution of mutations and mutation types across the KRTAP4-4 significant gene.

DNMT3A

Figure S22. This figure depicts the distribution of mutations and mutation types across the DNMT3A significant gene.

KRAS

Figure S23. This figure depicts the distribution of mutations and mutation types across the KRAS significant gene.

MSI1

Figure S24. This figure depicts the distribution of mutations and mutation types across the MSI1 significant gene.

FAM155A

Figure S25. This figure depicts the distribution of mutations and mutation types across the FAM155A significant gene.

COSMIC analyses

In this analysis, COSMIC is used as a filter to increase power by restricting the territory of each gene. Cosmic version: v48.

Table 4. Get Full Table Significantly mutated genes (COSMIC territory only). To access the database please go to: COSMIC. Number of significant genes found: 11. Number of genes displayed: 10

rank | gene | description | n | cos | n_cos | N_cos | cos_ev | p | q
--- | --- | --- | --- | --- | --- | --- | --- | --- | ---
1 | HRAS | v-Ha-ras Harvey rat sarcoma viral oncogene homolog | 12 | 19 | 12 | 6137 | 2496 | 2.5e-13 | 9.6e-10
2 | NRAS | neuroblastoma RAS viral (v-ras) oncogene homolog | 26 | 33 | 26 | 10659 | 33748 | 4.3e-13 | 9.6e-10
3 | BRAF | v-raf murine sarcoma viral oncogene homolog B1 | 183 | 89 | 183 | 28747 | 2630268 | 1.1e-12 | 1.7e-09
4 | KRAS | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | 3 | 52 | 3 | 16796 | 14910 | 1.3e-07 | 0.00015
5 | PIK3CA | phosphoinositide-3-kinase, catalytic, alpha polypeptide | 3 | 220 | 3 | 71060 | 51 | 9.7e-06 | 0.0088
6 | C4BPA | complement component 4 binding protein, alpha | 1 | 1 | 1 | 323 | 1 | 0.00018 | 0.073
7 | PCGF2 | polycomb group ring finger 2 | 2 | 1 | 1 | 323 | 1 | 0.00018 | 0.073
8 | SEZ6L | seizure related 6 homolog (mouse)-like | 2 | 1 | 1 | 323 | 1 | 0.00018 | 0.073
9 | SMC3 | structural maintenance of chromosomes 3 | 1 | 1 | 1 | 323 | 1 | 0.00018 | 0.073
10 | TNFRSF9 | tumor necrosis factor receptor superfamily, member 9 | 1 | 1 | 1 | 323 | 1 | 0.00018 | 0.073

Note:

n - number of (nonsilent) mutations in this gene across the individual set.

cos = number of unique mutated sites in this gene in COSMIC

n_cos = overlap between n and cos.

N_cos = number of individuals times cos.

cos_ev = total evidence: number of reports in COSMIC for mutations seen in this gene.

p = p-value for seeing the observed amount of overlap in this gene)

q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)

Clustered Mutations

Table 5. Get Full Table Genes with Clustered Mutations

num | gene | desc | n | mindist | nmuts0 | nmuts3 | nmuts12 | npairs0 | npairs3 | npairs12
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
353 | BRAF | v-raf murine sarcoma viral oncogene homolog B1 | 183 | 0 | 16471 | 16653 | 16653 | 16471 | 16653 | 16653
2198 | NRAS | neuroblastoma RAS viral (v-ras) oncogene homolog | 26 | 0 | 325 | 325 | 325 | 325 | 325 | 325
1472 | HRAS | v-Ha-ras Harvey rat sarcoma viral oncogene homolog | 12 | 0 | 66 | 66 | 66 | 66 | 66 | 66
541 | CCDC15 | coiled-coil domain containing 15 | 5 | 0 | 10 | 10 | 10 | 10 | 10 | 10
3779 | ZNF799 | zinc finger protein 799 | 5 | 0 | 6 | 6 | 6 | 6 | 6 | 6
3788 | ZNF845 | zinc finger protein 845 | 6 | 0 | 6 | 6 | 6 | 6 | 6 | 6
1015 | EIF1AX | eukaryotic translation initiation factor 1A, X-linked | 6 | 0 | 4 | 7 | 7 | 4 | 7 | 7
1124 | FAM47C | family with sequence similarity 47, member C | 5 | 0 | 3 | 3 | 3 | 3 | 3 | 3
3175 | SYNPO2L | synaptopodin 2-like | 3 | 0 | 3 | 3 | 3 | 3 | 3 | 3
3714 | ZNF443 | zinc finger protein 443 | 4 | 0 | 3 | 3 | 3 | 3 | 3 | 3

Note:

n - number of mutations in this gene in the individual set.

mindist - distance (in aa) between closest pair of mutations in this gene

npairs3 - how many pairs of mutations are within 3 aa of each other.

npairs12 - how many pairs of mutations are within 12 aa of each other.

Geneset Analyses

Table 6. Get Full Table A Ranked List of Significantly Mutated Genesets. (Source: MSigDB GSEA Cannonical Pathway Set).Number of significant genesets found: 72. Number of genesets displayed: 10

rank | geneset | description | genes | N_genes | mut_tally | N | n | npat | nsite | nsil | n1 | n2 | n3 | n4 | n5 | n6 | p_ns_s | p | q
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
1 | HSA04810_REGULATION_OF_ACTIN_CYTOSKELETON | Genes involved in regulation of actin cytoskeleton | ABI2, ACTN1, ACTN2, ACTN3, ACTN4, APC, APC2, ARAF, ARHGEF1, ARHGEF12, ARHGEF4, ARHGEF6, ARHGEF7, ARPC1A, ARPC1B, ARPC2, ARPC3, ARPC4, ARPC5, ARPC5L, BAIAP2, BCAR1, BDKRB1, BDKRB2, BRAF, C3orf10, CD14, CDC42, CFL1, CFL2, CHRM1, CHRM2, CHRM3, CHRM4, CHRM5, CRK, CRKL, CSK, CYFIP1, CYFIP2, DIAPH1, DIAPH2, DIAPH3, DOCK1, EGF, EGFR, EZR, F2, F2R, FGD1, FGD3, FGF1, FGF10, FGF11, FGF12, FGF13, FGF14, FGF16, FGF17, FGF18, FGF19, FGF2, FGF20, FGF21, FGF22, FGF23, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGFR1, FGFR2, FGFR3, FGFR4, FN1, GIT1, GNA12, GNA13, GNG12, GRLF1, GSN, HRAS, INS, IQGAP1, IQGAP2, IQGAP3, ITGA1, ITGA10, ITGA11, ITGA2, ITGA2B, ITGA3, ITGA4, ITGA5, ITGA6, ITGA7, ITGA8, ITGA9, ITGAD, ITGAE, ITGAL, ITGAM, ITGAV, ITGAX, ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, ITGB6, ITGB7, ITGB8, KRAS, LIMK1, LIMK2, LOC200025, LOC645126, LOC653888, MAP2K1, MAP2K2, MAPK1, MAPK3, MLCK, MOS, MRAS, MRCL3, MRLC2, MSN, MYH10, MYH14, MYH9, MYL2, MYL5, MYL7, MYL8P, MYL9, MYLC2PL, MYLK, MYLK2, MYLPF, NCKAP1, NCKAP1L, NRAS, PAK1, PAK2, PAK3, PAK4, PAK6, PAK7, PDGFA, PDGFB, PDGFRA, PDGFRB, PFN1, PFN2, PFN3, PFN4, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PIK3R5, PIP4K2A, PIP4K2B, PIP4K2C, PIP5K1A, PIP5K1B, PIP5K1C, PIP5K3, PPP1CA, PPP1CB, PPP1CC, PPP1R12A, PPP1R12B, PTK2, PXN, RAC1, RAC2, RAC3, RAF1, RDX, RHOA, ROCK1, ROCK2, RRAS, RRAS2, SCIN, SLC9A1, SOS1, SOS2, SSH1, SSH2, SSH3, TIAM1, TIAM2, TMSB4X, TMSB4Y, TMSL3, VAV1, VAV2, VAV3, VCL, WAS, WASF1, WASF2, WASL | 202 | ABI2(2), APC(1), ARHGEF4(1), ARHGEF6(1), ARHGEF7(2), BRAF(183), CDC42(1), CHRM3(1), CHRM4(1), CHRM5(1), EZR(1), FGD1(1), FGD3(1), FGF20(1), FGF7(1), FN1(1), HRAS(12), IQGAP1(2), ITGA10(1), ITGA3(2), ITGA8(1), ITGAD(2), ITGAL(4), ITGAM(2), ITGB1(2), ITGB3(1), ITGB4(1), ITGB8(1), KRAS(3), MYH10(3), MYH14(1), MYH9(1), MYLK(2), NCKAP1L(1), NRAS(26), PAK3(1), PAK7(1), PDGFRB(1), PIK3CA(3), PIK3CB(1), PIK3CG(1), PIK3R1(1), PIK3R5(3), PIP4K2B(1), PIP4K2C(1), PIP5K1A(1), SOS1(1), TIAM2(3), VAV2(1), VCL(1) | 133274235 | 289 | 243 | 73 | 27 | 9 | 18 | 38 | 210 | 14 | 0 | 3.89e-09 | <1.00e-15 | <1.50e-13
2 | ST_ADRENERGIC | Adrenergic receptors respond to epinephrine and norepinephrine signaling. | AKT1, APC, AR, ASAH1, BF, BRAF, CAMP, CCL13, CCL15, CCL16, DAG1, EGFR, GAS, GNA11, GNA15, GNAI1, GNAQ, ITPKA, ITPKB, ITPR1, ITPR2, ITPR3, KCNJ3, KCNJ5, KCNJ9, MAPK1, MAPK10, MAPK14, PHKA2, PIK3CA, PIK3CD, PIK3R1, PITX2, PTX1, PTX3, RAF1, SRC | 34 | APC(1), AR(1), BRAF(183), GNA15(1), ITPR1(2), ITPR2(4), MAPK10(1), PHKA2(2), PIK3CA(3), PIK3R1(1), PITX2(1), SRC(1) | 25744051 | 201 | 190 | 20 | 5 | 5 | 2 | 1 | 189 | 4 | 0 | 3.78e-11 | <1.00e-15 | <1.50e-13
3 | HSA04320_DORSO_VENTRAL_AXIS_FORMATION | Genes involved in dorso-ventral axis formation | BRAF, CPEB1, EGFR, ERBB2, ERBB4, ETS1, ETS2, ETV6, ETV7, FMN2, GRB2, KRAS, MAP2K1, MAPK1, MAPK3, NOTCH1, NOTCH2, NOTCH3, NOTCH4, PIWIL1, PIWIL2, PIWIL3, PIWIL4, RAF1, SOS1, SOS2, SPIRE1, SPIRE2 | 28 | BRAF(183), ERBB2(1), ETS1(2), KRAS(3), NOTCH2(1), NOTCH4(1), PIWIL2(1), SOS1(1) | 25185488 | 193 | 188 | 12 | 5 | 1 | 2 | 4 | 186 | 0 | 0 | 4.14e-11 | <1.00e-15 | <1.50e-13
4 | ST_G_ALPHA_S_PATHWAY | The G-alpha-s protein activates adenylyl cyclases, which catalyze cAMP formation. | ASAH1, BF, BFAR, BRAF, CAMP, CREB1, CREB3, CREB5, EPAC, GAS, GRF2, MAPK1, RAF1, SNX13, SRC, TERF2IP | 12 | BFAR(1), BRAF(183), SNX13(1), SRC(1), TERF2IP(1) | 5335473 | 187 | 184 | 6 | 2 | 0 | 0 | 1 | 183 | 3 | 0 | 2.63e-12 | 1.11e-15 | 1.50e-13
5 | HSA04730_LONG_TERM_DEPRESSION | Genes involved in long-term depression | ARAF, BRAF, C7orf16, CACNA1A, CRH, CRHR1, GNA11, GNA12, GNA13, GNAI1, GNAI2, GNAI3, GNAO1, GNAQ, GNAS, GNAZ, GRIA1, GRIA2, GRIA3, GRID2, GRM1, GRM5, GUCY1A2, GUCY1A3, GUCY1B3, GUCY2C, GUCY2D, GUCY2F, HRAS, IGF1, IGF1R, ITPR1, ITPR2, ITPR3, KRAS, LYN, MAP2K1, MAP2K2, MAPK1, MAPK3, NOS1, NOS2A, NOS3, NPR1, NPR2, NRAS, PLA2G10, PLA2G12A, PLA2G12B, PLA2G1B, PLA2G2A, PLA2G2D, PLA2G2E, PLA2G2F, PLA2G3, PLA2G4A, PLA2G5, PLA2G6, PLCB1, PLCB2, PLCB3, PLCB4, PPP2CA, PPP2CB, PPP2R1A, PPP2R1B, PPP2R2A, PPP2R2B, PPP2R2C, PRKCA, PRKCB1, PRKCG, PRKG1, PRKG2, RAF1, RYR1 | 74 | BRAF(183), CACNA1A(2), GNAS(3), GRIA1(1), GRIA2(2), GRM1(3), HRAS(12), IGF1R(1), ITPR1(2), ITPR2(4), KRAS(3), NPR1(1), NRAS(26), PLA2G5(1), PLCB2(1), PPP2R1A(2), PRKG1(1), RYR1(4) | 53048814 | 252 | 234 | 36 | 8 | 9 | 5 | 32 | 203 | 3 | 0 | 3.60e-14 | 1.22e-15 | 1.50e-13
6 | HSA04650_NATURAL_KILLER_CELL_MEDIATED_CYTOTOXICITY | Genes involved in natural killer cell mediated cytotoxicity | ARAF, BID, BRAF, CASP3, CD244, CD247, CD48, CHP, CSF2, FAS, FASLG, FCER1G, FCGR3A, FCGR3B, FYN, GRB2, GZMB, HCST, HLA-A, HLA-B, HLA-C, HLA-E, HLA-G, HRAS, ICAM1, ICAM2, IFNA1, IFNA10, IFNA13, IFNA14, IFNA16, IFNA17, IFNA2, IFNA21, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNAR1, IFNAR2, IFNB1, IFNG, IFNGR1, IFNGR2, ITGAL, ITGB2, KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR2DL5A, KIR2DS1, KIR2DS2, KIR3DL1, KIR3DL2, KLRC1, KLRC2, KLRC3, KLRD1, KLRK1, KRAS, LAT, LCK, LCP2, LOC652578, MAP2K1, MAP2K2, MAPK1, MAPK3, MICA, MICB, NCR1, NCR2, NCR3, NFAT5, NFATC1, NFATC2, NFATC3, NFATC4, NRAS, PAK1, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PIK3R5, PLCG1, PLCG2, PPP3CA, PPP3CB, PPP3CC, PPP3R1, PPP3R2, PRF1, PRKCA, PRKCB1, PRKCG, PTK2B, PTPN11, PTPN6, RAC1, RAC2, RAC3, RAF1, SH2D1A, SH2D1B, SH3BP2, SHC1, SHC2, SHC3, SHC4, SOS1, SOS2, SYK, TNF, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFSF10, TYROBP, ULBP1, ULBP2, ULBP3, VAV1, VAV2, VAV3, ZAP70 | 126 | BRAF(183), FYN(1), HLA-E(1), HRAS(12), IFNA4(1), IFNAR2(1), IFNGR1(2), ITGAL(4), KIR2DL1(1), KIR3DL1(2), KRAS(3), LCK(1), NCR1(1), NFAT5(1), NFATC1(2), NFATC4(2), NRAS(26), PIK3CA(3), PIK3CB(1), PIK3CG(1), PIK3R1(1), PIK3R5(3), PLCG2(1), PPP3R2(1), PTK2B(1), SHC1(1), SOS1(1), SYK(1), VAV2(1) | 54658054 | 260 | 231 | 45 | 13 | 7 | 7 | 35 | 205 | 6 | 0 | 1.44e-12 | 2.00e-15 | 1.57e-13
7 | ST_G_ALPHA_I_PATHWAY | Gi and Go proteins are members of the same family that transduce cellular signals through both their alpha and beta subunits. | AKT1, AKT2, AKT3, ASAH1, BF, BRAF, DAG1, DRD2, EGFR, EPHB2, GRB2, ITPKA, ITPKB, ITPR1, ITPR2, ITPR3, KCNJ3, KCNJ5, KCNJ9, MAPK1, PI3, PIK3CB, PITX2, PLCB1, PLCB2, PLCB3, PLCB4, RAF1, RAP1GA1, RGS20, SHC1, SOS1, SOS2, SRC, STAT3, TERF2IP | 34 | BRAF(183), ITPR1(2), ITPR2(4), PIK3CB(1), PITX2(1), PLCB2(1), SHC1(1), SOS1(1), SRC(1), TERF2IP(1) | 28143487 | 196 | 189 | 15 | 3 | 6 | 2 | 1 | 186 | 1 | 0 | 1.17e-12 | 2.11e-15 | 1.57e-13
8 | HSA04010_MAPK_SIGNALING_PATHWAY | Genes involved in MAPK signaling pathway | ACVR1B, ACVR1C, AKT1, AKT2, AKT3, ARRB1, ARRB2, ATF2, ATF4, BDNF, BRAF, CACNA1A, CACNA1B, CACNA1C, CACNA1D, CACNA1E, CACNA1F, CACNA1G, CACNA1H, CACNA1I, CACNA1S, CACNA2D1, CACNA2D2, CACNA2D3, CACNA2D4, CACNB1, CACNB2, CACNB3, CACNB4, CACNG1, CACNG2, CACNG3, CACNG4, CACNG5, CACNG6, CACNG7, CACNG8, CASP3, CD14, CDC25B, CDC42, CHP, CHUK, CRK, CRKL, DAXX, DDIT3, DUSP1, DUSP10, DUSP14, DUSP16, DUSP2, DUSP3, DUSP4, DUSP5, DUSP6, DUSP7, DUSP8, DUSP9, ECSIT, EGF, EGFR, ELK1, ELK4, EVI1, FAS, FASLG, FGF1, FGF10, FGF11, FGF12, FGF13, FGF14, FGF16, FGF17, FGF18, FGF19, FGF2, FGF20, FGF21, FGF22, FGF23, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGFR1, FGFR2, FGFR3, FGFR4, FLNA, FLNB, FLNC, FOS, GADD45A, GADD45B, GADD45G, GNA12, GNG12, GRB2, HRAS, IKBKB, IKBKG, IL1A, IL1B, IL1R1, IL1R2, JUN, JUND, KRAS, LOC653852, MAP2K1, MAP2K1IP1, MAP2K2, MAP2K3, MAP2K4, MAP2K5, MAP2K6, MAP2K7, MAP3K1, MAP3K10, MAP3K12, MAP3K13, MAP3K14, MAP3K2, MAP3K3, MAP3K4, MAP3K5, MAP3K6, MAP3K7, MAP3K7IP1, MAP3K7IP2, MAP3K8, MAP4K1, MAP4K2, MAP4K3, MAP4K4, MAPK1, MAPK10, MAPK11, MAPK12, MAPK13, MAPK14, MAPK3, MAPK7, MAPK8, MAPK8IP1, MAPK8IP2, MAPK8IP3, MAPK9, MAPKAPK2, MAPKAPK3, MAPKAPK5, MAPT, MAX, MEF2C, MKNK1, MKNK2, MOS, MRAS, MYC, NF1, NFATC2, NFATC4, NFKB1, NFKB2, NGFB, NLK, NR4A1, NRAS, NTF3, NTF5, NTRK1, NTRK2, PAK1, PAK2, PDGFA, PDGFB, PDGFRA, PDGFRB, PLA2G10, PLA2G12A, PLA2G12B, PLA2G1B, PLA2G2A, PLA2G2D, PLA2G2E, PLA2G2F, PLA2G3, PLA2G4A, PLA2G5, PLA2G6, PPM1A, PPM1B, PPP3CA, PPP3CB, PPP3CC, PPP3R1, PPP3R2, PPP5C, PRKACA, PRKACB, PRKACG, PRKCA, PRKCB1, PRKCG, PRKX, PRKY, PTPN5, PTPN7, PTPRR, RAC1, RAC2, RAC3, RAF1, RAP1A, RAP1B, RAPGEF2, RASA1, RASA2, RASGRF1, RASGRF2, RASGRP1, RASGRP2, RASGRP3, RASGRP4, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA4, RPS6KA5, RPS6KA6, RRAS, RRAS2, SOS1, SOS2, SRF, STK3, STK4, STMN1, TAOK1, TAOK2, TAOK3, TGFB1, TGFB2, TGFB3, TGFBR1, TGFBR2, TNF, TNFRSF1A, TP53, TRAF2, TRAF6, ZAK | 247 | ARRB1(1), BRAF(183), CACNA1A(2), CACNA1B(1), CACNA1C(1), CACNA1D(1), CACNA1E(3), CACNA1G(2), CACNA1H(1), CACNA1S(1), CACNA2D1(2), CACNA2D3(1), CACNA2D4(1), CACNB3(1), CDC42(1), DUSP2(1), DUSP5(1), DUSP7(1), DUSP8(1), ECSIT(1), FGF20(1), FGF7(1), FLNA(1), FLNC(3), HRAS(12), IL1R1(2), JUN(1), KRAS(3), MAP2K5(1), MAP2K6(1), MAP3K1(1), MAP3K3(4), MAP3K6(1), MAP3K8(1), MAP4K4(1), MAPK10(1), MAPK8IP2(1), MAPKAPK3(1), MKNK2(1), MYC(1), NF1(5), NFATC4(2), NRAS(26), PDGFRB(1), PLA2G5(1), PPP3R2(1), RASGRF2(1), RASGRP1(1), RPS6KA1(1), SOS1(1), TGFB1(1), TP53(3) | 137756696 | 290 | 242 | 75 | 23 | 15 | 14 | 37 | 208 | 16 | 0 | 3.98e-11 | 2.22e-15 | 1.57e-13
9 | HSA04150_MTOR_SIGNALING_PATHWAY | Genes involved in mTOR signaling pathway | AKT1, AKT2, AKT3, BRAF, CAB39, DDIT4, EIF4B, EIF4EBP1, FIGF, FRAP1, GBL, HIF1A, IGF1, INS, KIAA1303, LYK5, MAPK1, MAPK3, PDPK1, PGF, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PIK3R5, PRKAA1, PRKAA2, RHEB, RICTOR, RPS6, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA6, RPS6KB1, RPS6KB2, STK11, TSC1, TSC2, ULK1, ULK2, ULK3, VEGFA, VEGFB, VEGFC | 44 | BRAF(183), FIGF(1), PIK3CA(3), PIK3CB(1), PIK3CG(1), PIK3R1(1), PIK3R5(3), RPS6KA1(1), RPS6KB2(1), ULK3(1), VEGFA(1) | 25626228 | 197 | 186 | 16 | 4 | 1 | 2 | 3 | 187 | 4 | 0 | 1.50e-11 | 2.33e-15 | 1.57e-13
10 | ST_DIFFERENTIATION_PATHWAY_IN_PC12_CELLS | Rat-derived PC12 cells respond to nerve growth factor (NGF) and PACAP to differentiate into neuronal cells. | AKT1, ASAH1, ATF1, BRAF, CAMP, CREB1, CREB3, CREB5, CREBBP, CRKL, DAG1, EGR1, EGR2, EGR3, EGR4, ELK1, FRS2, GAS, GNAQ, GRF2, JUN, MAP1B, MAP2K4, MAP2K7, MAPK1, MAPK10, MAPK3, MAPK8, MAPK8IP1, MAPK8IP2, MAPK8IP3, MAPK9, NTRK1, OPN1LW, PACAP, PIK3C2G, PIK3CA, PIK3CD, PIK3R1, PTPN11, RPS6KA3, SH2B, SHC1, SRC, TERF2IP, TH, TUBA3 | 42 | BRAF(183), CREBBP(1), JUN(1), MAP1B(2), MAPK10(1), MAPK8IP2(1), PIK3C2G(1), PIK3CA(3), PIK3R1(1), SHC1(1), SRC(1), TERF2IP(1) | 24780522 | 197 | 186 | 16 | 4 | 3 | 3 | 1 | 187 | 3 | 0 | 2.22e-12 | 2.55e-15 | 1.57e-13

Table 7. Get Full Table A Ranked List of Significantly Mutated Genesets (Excluding Significantly Mutated Genes). Number of significant genesets found: 0. Number of genesets displayed: 10

rank | geneset | description | genes | N_genes | mut_tally | N | n | npat | nsite | nsil | n1 | n2 | n3 | n4 | n5 | n6 | p_ns_s | p | q
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
1 | ATMPATHWAY | The tumor-suppressing protein kinase ATM responds to radiation-induced DNA damage by blocking cell-cycle progression and activating DNA repair. | ABL1, ATM, BRCA1, CDKN1A, CHEK1, CHEK2, GADD45A, JUN, MAPK8, MDM2, MRE11A, NBS1, NFKB1, NFKBIA, RAD50, RAD51, RBBP8, RELA, TP53, TP73 | 19 | ABL1(1), ATM(5), BRCA1(1), CHEK1(1), CHEK2(2), JUN(1), RAD51(1), RBBP8(1), TP53(3), TP73(3) | 14326099 | 19 | 18 | 19 | 0 | 1 | 6 | 2 | 3 | 7 | 0 | 0.0071 | 0.00038 | 0.24
2 | PLK3PATHWAY | Active Plk3 phosphorylates CDC25c, blocking the G2/M transition, and phosphorylates p53 to induce apoptosis. | ATM, ATR, CDC25C, CHEK1, CHEK2, CNK, TP53, YWHAH | 7 | ATM(5), ATR(1), CHEK1(1), CHEK2(2), TP53(3) | 7677248 | 12 | 12 | 12 | 1 | 0 | 1 | 1 | 3 | 7 | 0 | 0.27 | 0.00098 | 0.3
3 | ARFPATHWAY | Cyclin-dependent kinase inhibitor 2A is a tumor suppressor that induces G1 arrest and can activate the p53 pathway, leading to G2/M arrest. | ABL1, CDKN2A, E2F1, MDM2, MYC, PIK3CA, PIK3R1, POLR1A, POLR1B, POLR1C, POLR1D, RAC1, RB1, TBX2, TP53, TWIST1 | 16 | ABL1(1), MYC(1), PIK3CA(3), PIK3R1(1), POLR1A(1), POLR1B(2), TP53(3), TWIST1(1) | 9760763 | 13 | 13 | 13 | 0 | 1 | 2 | 1 | 2 | 7 | 0 | 0.032 | 0.003 | 0.6
4 | TERTPATHWAY | hTERC, the RNA subunit of telomerase, and hTERT, the catalytic protein subunit, are required for telomerase activity and are overexpressed in many cancers. | HDAC1, MAX, MYC, SP1, SP3, TP53, WT1, ZNF42 | 7 | MYC(1), SP1(1), TP53(3), WT1(1) | 3398513 | 6 | 6 | 6 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0.16 | 0.0088 | 1
5 | HSA00760_NICOTINATE_AND_NICOTINAMIDE_METABOLISM | Genes involved in nicotinate and nicotinamide metabolism | AOX1, BST1, C9orf95, CD38, ENPP1, ENPP3, NADK, NADSYN1, NMNAT1, NMNAT2, NMNAT3, NNMT, NNT, NP, NT5C, NT5C1A, NT5C1B, NT5C2, NT5C3, NT5E, NT5M, NUDT12, PBEF1, QPRT | 22 | AOX1(1), C9orf95(1), CD38(1), ENPP1(2), NADK(1), NADSYN1(1), NNT(1), NT5C3(1), NT5E(1), NUDT12(1), QPRT(1) | 10362895 | 12 | 11 | 12 | 1 | 4 | 2 | 4 | 1 | 1 | 0 | 0.069 | 0.01 | 1
6 | CTLA4PATHWAY | T cell activation requires interaction with an antigen-MHC-I complex on an antigen-presenting cell (APC), as well as CD28 interaction with the APC's CD80 or 86. | CD28, CD3D, CD3E, CD3G, CD3Z, CD80, CD86, CTLA4, GRB2, HLA-DRA, HLA-DRB1, ICOS, ICOSL, IL2, ITK, LCK, PIK3CA, PIK3R1, PTPN11, TRA@, TRB@ | 17 | CD28(1), CD3D(1), HLA-DRA(1), LCK(1), PIK3CA(3), PIK3R1(1) | 6150701 | 8 | 8 | 8 | 0 | 2 | 1 | 2 | 2 | 1 | 0 | 0.12 | 0.012 | 1
7 | TCRAPATHWAY | The kinases Lck and Fyn phosphorylate and activate the T cell receptor, which recognizes antigen-bound MHCII and leads to T cell activation. | CD3D, CD3E, CD3G, CD3Z, CD4, FYN, HLA-DRA, HLA-DRB1, LCK, PTPRC, TRA@, TRB@, ZAP70 | 10 | CD3D(1), CD4(1), FYN(1), HLA-DRA(1), LCK(1), PTPRC(1) | 4309898 | 6 | 6 | 6 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 0.15 | 0.017 | 1
8 | BBCELLPATHWAY | Fas ligand expression by T cells induces apoptosis in Fas-expressing, inactive B cells. | CD28, CD4, HLA-DRA, HLA-DRB1, TNFRSF5, TNFRSF6, TNFSF5, TNFSF6 | 4 | CD28(1), CD4(1), HLA-DRA(1) | 1139847 | 3 | 3 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0.36 | 0.018 | 1
9 | TCAPOPTOSISPATHWAY | HIV infection upregulates Fas ligand in macrophages and CD4 in helper T cells, leading to widespread Fas-induced T cell apoptosis. | CCR5, CD28, CD3D, CD3E, CD3G, CD3Z, CD4, TNFRSF6, TNFSF6, TRA@, TRB@ | 6 | CD28(1), CD3D(1), CD4(1) | 1545412 | 3 | 3 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0.35 | 0.018 | 1
10 | P53PATHWAY | p53 induces cell cycle arrest or apoptosis under conditions of DNA damage. | APAF1, ATM, BAX, BCL2, CCND1, CCNE1, CDK2, CDK4, CDKN1A, E2F1, GADD45A, MDM2, PCNA, RB1, TIMP3, TP53 | 16 | APAF1(2), ATM(5), TP53(3) | 8702713 | 10 | 10 | 10 | 0 | 0 | 1 | 1 | 2 | 6 | 0 | 0.14 | 0.019 | 1

Methods & Data
Methods

In brief, we tabulate the number of mutations and the number of covered bases for each gene. The counts are broken down by mutation context category: four context categories that are discovered by MutSig, and one for indel and 'null' mutations, which include indels, nonsense mutations, splice-site mutations, and non-stop (read-through) mutations. For each gene, we calculate the probability of seeing the observed constellation of mutations, i.e. the product P1 x P2 x ... x Pm, or a more extreme one, given the background mutation rates calculated across the dataset. [1]

Download Results

This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.

- Analysis Results (MD5 checksum)

- Auxiliary Data (MD5 checksum)

- MAGE-TAB File (MD5 checksum)

References
[1] TCGA, Integrated genomic analyses of ovarian carcinoma, Nature 474:609 - 615 (2011)
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