This pipeline uses various statistical tests to identify mRNAs whose expression levels correlated to selected clinical features.
Testing the association between 17848 genes and 4 clinical features across 60 samples, statistically thresholded by Q value < 0.05, 2 clinical features related to at least one genes.
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8 genes correlated to 'GENDER'.
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XIST|7503 , TSIX|9383 , ZFY|7544 , KDM5C|8242 , EIF1AX|1964 , ...
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1 gene correlated to 'COMPLETENESS.OF.RESECTION'.
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SLC16A11|162515
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No genes correlated to 'Time to Death', and 'AGE'.
Complete statistical result table is provided in Supplement Table 1
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
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Time to Death | Cox regression test | N=0 | ||||
AGE | Spearman correlation test | N=0 | ||||
GENDER | t test | N=8 | male | N=4 | female | N=4 |
COMPLETENESS OF RESECTION | ANOVA test | N=1 |
Time to Death | Duration (Months) | 0.1-83.6 (median=14.3) |
censored | N = 32 | |
death | N = 23 | |
Significant markers | N = 0 |
AGE | Mean (SD) | 59.66 (15) |
Significant markers | N = 0 |
GENDER | Labels | N |
FEMALE | 23 | |
MALE | 37 | |
Significant markers | N = 8 | |
Higher in MALE | 4 | |
Higher in FEMALE | 4 |
T(pos if higher in 'MALE') | ttestP | Q | AUC | |
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XIST|7503 | -14.22 | 1.96e-15 | 3.5e-11 | 0.9861 |
TSIX|9383 | -9.48 | 5.074e-10 | 9.05e-06 | 0.9814 |
ZFY|7544 | 12.05 | 2.187e-08 | 0.00039 | 0.9919 |
KDM5C|8242 | -6.19 | 6.706e-08 | 0.0012 | 0.8719 |
EIF1AX|1964 | -6.31 | 7.425e-08 | 0.00132 | 0.8754 |
NLGN4Y|22829 | 9.56 | 1.155e-07 | 0.00206 | 0.9811 |
PRKY|5616 | 9.24 | 6.111e-07 | 0.0109 | 0.9842 |
TERF1|7013 | 5.46 | 1.228e-06 | 0.0219 | 0.8496 |
COMPLETENESS.OF.RESECTION | Labels | N |
R0 | 43 | |
R1 | 7 | |
R2 | 1 | |
RX | 6 | |
Significant markers | N = 1 |
ANOVA_P | Q | |
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SLC16A11|162515 | 5.168e-08 | 0.000922 |
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Expresson data file = LIHC-TP.uncv2.mRNAseq_RSEM_normalized_log2.txt
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Clinical data file = LIHC-TP.clin.merged.picked.txt
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Number of patients = 60
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Number of genes = 17848
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Number of clinical features = 4
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.