Analysis Overview
Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (Primary solid tumor)
23 May 2013  |  analyses__2013_05_23
Maintainer Information
Citation Information
Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)
Cite as Broad Institute TCGA Genome Data Analysis Center (2013): Analysis Overview for Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (Primary solid tumor cohort) - 23 May 2013. Broad Institute of MIT and Harvard. doi:10.7908/C10K26KS
Overview
Introduction

This is an overview of Lymphoid Neoplasm Diffuse Large B-cell Lymphoma analysis pipelines from Firehose run "23 May 2013".

Summary

Note: These results are offered to the community as an additional reference point, enabling a wide range of cancer biologists, clinical investigators, and genome and computational scientists to easily incorporate TCGA into the backdrop of ongoing research. While every effort is made to ensure that Firehose input data and algorithms are of the highest possible quality, these analyses have not been reviewed by domain experts.

Results
  • Sequence and Copy Number Analyses

    • Copy number analysis (GISTIC2)
      View Report | There were 18 tumor samples used in this analysis: 8 significant arm-level results, 1 significant focal amplifications, and 12 significant focal deletions were found.

  • Clustering Analyses

    • Clustering of Methylation: consensus NMF
      View Report | The 16037 most variable methylated genes were selected based on variation. The variation cutoff are set for each tumor type empirically by fitting a bimodal distriution. For genes with multiple methylation probes, we chose the most variable one to represent the gene. Consensus NMF clustering of 18 samples and 16037 genes identified 2 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

  • Other Correlation Analyses

    • Correlation between copy number variation genes and molecular subtypes
      View Report | Testing the association between copy number variation of 9 peak regions and molecular subtype 'METHLYATION_CNMF' across 18 patients, one significant finding detected with Q value < 0.25.

    • Correlation between copy number variations of arm-level result and molecular subtypes
      View Report | Testing the association between copy number variation 7 arm-level results and molecular subtype 'METHLYATION_CNMF' across 18 patients, no significant finding detected with Q value < 0.25.

Methods & Data
Input
  • Summary Report Date = Wed Jun 26 16:23:55 2013

  • Protection = FALSE