Analysis Overview
Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (Primary solid tumor)
15 January 2014 | analyses__2014_01_15
Maintainer Information
Citation Information
doi:10.7908/C1GH9GB8
Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)
Cite as Broad Institute TCGA Genome Data Analysis Center (2014): Analysis Overview for Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (Primary solid tumor cohort) - 15 January 2014. Broad Institute of MIT and Harvard. doi:10.7908/C1GH9GB8
Overview
Introduction

This is an overview of Lymphoid Neoplasm Diffuse Large B-cell Lymphoma analysis pipelines from Firehose run "15 January 2014".

Summary

Note: These results are offered to the community as an additional reference point, enabling a wide range of cancer biologists, clinical investigators, and genome and computational scientists to easily incorporate TCGA into the backdrop of ongoing research. While every effort is made to ensure that Firehose input data and algorithms are of the highest possible quality, these analyses have not been reviewed by domain experts.

Results

- Sequence and Copy Number Analyses

- SNP6 Copy number analysis (GISTIC2)
  View Report | There were 28 tumor samples used in this analysis: 9 significant arm-level results, 7 significant focal amplifications, and 19 significant focal deletions were found.

- Correlations to Clinical Parameters

- Correlation between aggregated molecular cancer subtypes and selected clinical features
  View Report | Testing the association between subtypes identified by 3 different clustering approaches and 3 clinical features across 21 patients, 2 significant findings detected with P value < 0.05 and Q value < 0.25.

- Correlation between copy number variation genes (focal events) and selected clinical features
  View Report | Testing the association between copy number variation 21 focal events and 3 clinical features across 21 patients, one significant finding detected with Q value < 0.25.

- Correlation between copy number variations of arm-level result and selected clinical features
  View Report | Testing the association between copy number variation 17 arm-level events and 3 clinical features across 21 patients, one significant finding detected with Q value < 0.25.

- Correlation between gene methylation status and clinical features
  View Report | Testing the association between 19739 genes and 3 clinical features across 21 samples, statistically thresholded by Q value < 0.05, no clinical feature related to at least one genes.

- Correlation between mRNAseq expression and clinical features
  View Report | Testing the association between 17576 genes and 3 clinical features across 21 samples, statistically thresholded by Q value < 0.05, 1 clinical feature related to at least one genes.

- Clustering Analyses

- Clustering of Methylation: consensus NMF
  View Report | The 14487 most variable methylated genes were selected based on variation. The variation cutoff are set for each tumor type empirically by fitting a bimodal distriution. For genes with multiple methylation probes, we chose the most variable one to represent the gene. Consensus NMF clustering of 28 samples and 14487 genes identified 3 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

- Clustering of mRNAseq gene expression: consensus hierarchical
  View Report | The 1500 most variable genes were selected. Consensus average linkage hierarchical clustering of 28 samples and 1500 genes identified 2 subtypes with the stability of the clustering increasing for k = 2 to k = 8 and the average silhouette width calculation for selecting the robust clusters.

- Clustering of mRNAseq gene expression: consensus NMF
  View Report | The most robust consensus NMF clustering of 28 samples using the 1500 most variable genes was identified for k = 2 clusters. We computed the clustering for k = 2 to k = 8 and used the cophenetic correlation coefficient to determine the best solution.

- Pathway Analyses

- PARADIGM pathway analysis of mRNASeq expression and copy number data
  View Report | There were 26 significant pathways identified in this analysis.

- PARADIGM pathway analysis of mRNASeq expression data
  View Report | There were 39 significant pathways identified in this analysis.

- Other Correlation Analyses

- Correlation between copy number variation genes (focal events) and molecular subtypes
  View Report | Testing the association between copy number variation 29 focal events and 3 molecular subtypes across 28 patients, no significant finding detected with P value < 0.05 and Q value < 0.25.

- Correlation between copy number variations of arm-level result and molecular subtypes
  View Report | Testing the association between copy number variation 24 arm-level events and 3 molecular subtypes across 28 patients, no significant finding detected with P value < 0.05 and Q value < 0.25.

- Correlation between mRNA expression and DNA methylation
  View Report | The top 25 correlated methylation probes per gene are displayed. Total number of matched samples = 28. Number of gene expression samples = 28. Number of methylation samples = 28.

- Correlations between copy number and mRNAseq expression
  View Report | The correlation coefficients in 10, 20, 30, 40, 50, 60, 70, 80, 90 percentiles are 773, 1738.4, 2909, 3579.8, 4207, 4834, 5508.4, 6238, 7073, respectively.

Methods & Data
Input

- Summary Report Date = Fri Feb 28 12:09:06 2014

- Protection = FALSE

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