Correlation between gene mutation status and selected clinical features
Kidney Chromophobe (Primary solid tumor)
15 January 2014 | analyses__2014_01_15
Maintainer Information
Citation Information
doi:10.7908/C16H4FX3
Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)
Cite as Broad Institute TCGA Genome Data Analysis Center (2014): Correlation between gene mutation status and selected clinical features. Broad Institute of MIT and Harvard. doi:10.7908/C16H4FX3
Overview
Introduction

This pipeline computes the correlation between significantly recurrent gene mutations and selected clinical features.

Summary

Testing the association between mutation status of 6 genes and 10 clinical features across 66 patients, no significant finding detected with Q value < 0.25.

- No gene mutations related to clinical features.

Results
Overview of the results

Table 1. Get Full Table Overview of the association between mutation status of 6 genes and 10 clinical features. Shown in the table are P values (Q values). Thresholded by Q value < 0.25, no significant finding detected.

|  | Clinical Features | Time to Death | AGE | NEOPLASM DISEASESTAGE | PATHOLOGY T STAGE | PATHOLOGY N STAGE | PATHOLOGY M STAGE | GENDER | KARNOFSKY PERFORMANCE SCORE | NUMBERPACKYEARSSMOKED | YEAROFTOBACCOSMOKINGONSET
| nMutated (%) | nWild-Type | logrank test | t-test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | t-test | t-test | t-test
MUC2 | 8 (12%) | 58 | 0.201 (1.00) | 0.368 (1.00) | 0.023 (1.00) | 0.152 (1.00) | 0.166 (1.00) | 0.488 (1.00) | 0.455 (1.00) |  |  |
MUC6 | 21 (32%) | 45 | 0.25 (1.00) | 0.641 (1.00) | 0.705 (1.00) | 0.523 (1.00) | 1 (1.00) | 0.535 (1.00) | 0.0304 (1.00) |  | 0.721 (1.00) | 0.0636 (1.00)
TP53 | 22 (33%) | 44 | 0.0075 (0.353) | 0.841 (1.00) | 0.293 (1.00) | 0.362 (1.00) | 0.036 (1.00) | 0.108 (1.00) | 1 (1.00) |  | 0.259 (1.00) | 0.777 (1.00)
PTEN | 6 (9%) | 60 | 0.00719 (0.345) | 0.438 (1.00) | 0.05 (1.00) | 0.0321 (1.00) | 0.0874 (1.00) | 0.658 (1.00) | 0.217 (1.00) |  |  |
PRSS3 | 7 (11%) | 59 | 0.667 (1.00) | 0.812 (1.00) | 0.102 (1.00) | 0.157 (1.00) | 1 (1.00) | 1 (1.00) | 0.432 (1.00) |  |  |
HLA-C | 7 (11%) | 59 | 0.289 (1.00) | 0.424 (1.00) | 0.836 (1.00) | 0.543 (1.00) | 1 (1.00) | 0.658 (1.00) | 0.432 (1.00) |  | 0.652 (1.00) | 0.0636 (1.00)

Methods & Data
Input

- Mutation data file = transformed.cor.cli.txt

- Clinical data file = KICH-TP.merged_data.txt

- Number of patients = 66

- Number of significantly mutated genes = 6

- Number of selected clinical features = 10

- Exclude genes that fewer than K tumors have mutations, K = 3

Survival analysis

For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R

Student's t-test analysis

For continuous numerical clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the clinical values between tumors with and without gene mutations using 't.test' function in R

Fisher's exact test

For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R

Q value calculation

For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.

Download Results

In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.

- Analysis Results (MD5 checksum)

- Auxiliary Data (MD5 checksum)

- MAGE-TAB File (MD5 checksum)

References
[1] Bland and Altman, Statistics notes: The logrank test, BMJ 328(7447):1073 (2004)
[2] Lehmann and Romano, Testing Statistical Hypotheses (3E ed.), New York: Springer. ISBN 0387988645 (2005)
[3] Fisher, R.A., On the interpretation of chi-square from contingency tables, and the calculation of P, Journal of the Royal Statistical Society 85(1):87-94 (1922)
[4] Benjamini and Hochberg, Controlling the false discovery rate: a practical and powerful approach to multiple testing, Journal of the Royal Statistical Society Series B 59:289-300 (1995)
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