This pipeline computes the correlation between cancer subtypes identified by different molecular patterns and selected clinical features.
Testing the association between subtypes identified by 12 different clustering approaches and 9 clinical features across 507 patients, 39 significant findings detected with P value < 0.05 and Q value < 0.25.
-
CNMF clustering analysis on array-based mRNA expression data identified 3 subtypes that do not correlate to any clinical features.
-
Consensus hierarchical clustering analysis on array-based mRNA expression data identified 3 subtypes that do not correlate to any clinical features.
-
3 subtypes identified in current cancer cohort by 'Copy Number Ratio CNMF subtypes'. These subtypes correlate to 'Time to Death', 'AGE', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', and 'PATHOLOGY.M.STAGE'.
-
3 subtypes identified in current cancer cohort by 'METHLYATION CNMF'. These subtypes correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', 'PATHOLOGY.M.STAGE', and 'GENDER'.
-
CNMF clustering analysis on RPPA data identified 6 subtypes that correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', 'PATHOLOGY.N.STAGE', and 'PATHOLOGY.M.STAGE'.
-
Consensus hierarchical clustering analysis on RPPA data identified 3 subtypes that correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', and 'PATHOLOGY.M.STAGE'.
-
CNMF clustering analysis on sequencing-based mRNA expression data identified 3 subtypes that correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', 'PATHOLOGY.M.STAGE', and 'GENDER'.
-
Consensus hierarchical clustering analysis on sequencing-based mRNA expression data identified 3 subtypes that correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', 'PATHOLOGY.N.STAGE', 'PATHOLOGY.M.STAGE', and 'GENDER'.
-
3 subtypes identified in current cancer cohort by 'MIRSEQ CNMF'. These subtypes correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', 'PATHOLOGY.M.STAGE', and 'GENDER'.
-
3 subtypes identified in current cancer cohort by 'MIRSEQ CHIERARCHICAL'. These subtypes do not correlate to any clinical features.
-
3 subtypes identified in current cancer cohort by 'MIRseq Mature CNMF subtypes'. These subtypes correlate to 'Time to Death', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.T.STAGE', and 'GENDER'.
-
5 subtypes identified in current cancer cohort by 'MIRseq Mature cHierClus subtypes'. These subtypes do not correlate to any clinical features.
Clinical Features |
Time to Death |
AGE |
NEOPLASM DISEASESTAGE |
PATHOLOGY T STAGE |
PATHOLOGY N STAGE |
PATHOLOGY M STAGE |
GENDER |
KARNOFSKY PERFORMANCE SCORE |
NUMBERPACKYEARSSMOKED |
Statistical Tests | logrank test | ANOVA | Chi-square test | Chi-square test | Chi-square test | Chi-square test | Chi-square test | ANOVA | ANOVA |
mRNA CNMF subtypes |
0.0843 (1.00) |
0.791 (1.00) |
0.00527 (0.29) |
0.00911 (0.464) |
0.0704 (1.00) |
0.12 (1.00) |
0.634 (1.00) |
||
mRNA cHierClus subtypes |
0.313 (1.00) |
0.647 (1.00) |
0.00728 (0.386) |
0.0125 (0.558) |
0.124 (1.00) |
0.104 (1.00) |
0.68 (1.00) |
||
Copy Number Ratio CNMF subtypes |
0.00014 (0.00991) |
0.00193 (0.118) |
3.98e-05 (0.00302) |
0.000984 (0.064) |
0.0121 (0.557) |
0.000656 (0.0446) |
0.00833 (0.433) |
0.722 (1.00) |
|
METHLYATION CNMF |
5.35e-06 (0.000423) |
0.0063 (0.34) |
1.47e-11 (1.34e-09) |
5.4e-12 (4.97e-10) |
0.169 (1.00) |
0.000759 (0.0509) |
0.000957 (0.0632) |
0.595 (1.00) |
|
RPPA CNMF subtypes |
1.71e-10 (1.52e-08) |
0.13 (1.00) |
1.2e-08 (1.06e-06) |
9.52e-07 (7.81e-05) |
0.00106 (0.0681) |
6.21e-07 (5.16e-05) |
0.0611 (1.00) |
0.322 (1.00) |
|
RPPA cHierClus subtypes |
1.02e-07 (8.85e-06) |
0.00939 (0.47) |
2e-06 (0.000162) |
4.03e-07 (3.39e-05) |
0.241 (1.00) |
0.000108 (0.00788) |
0.571 (1.00) |
0.0463 (1.00) |
|
RNAseq CNMF subtypes |
3.31e-07 (2.85e-05) |
0.193 (1.00) |
3.81e-07 (3.24e-05) |
1.46e-05 (0.00114) |
0.0112 (0.539) |
0.00129 (0.0811) |
4.81e-05 (0.0036) |
0.656 (1.00) |
|
RNAseq cHierClus subtypes |
3.05e-11 (2.74e-09) |
0.256 (1.00) |
2.65e-13 (2.49e-11) |
2.3e-12 (2.14e-10) |
0.00202 (0.121) |
1.47e-05 (0.00114) |
0.000495 (0.0341) |
0.35 (1.00) |
|
MIRSEQ CNMF |
3.45e-06 (0.000276) |
0.111 (1.00) |
0.000111 (0.00797) |
0.000458 (0.0321) |
0.0113 (0.539) |
0.0023 (0.133) |
0.00203 (0.121) |
0.155 (1.00) |
|
MIRSEQ CHIERARCHICAL |
0.0105 (0.512) |
0.0234 (0.983) |
0.127 (1.00) |
0.0236 (0.983) |
0.0134 (0.575) |
0.745 (1.00) |
0.363 (1.00) |
0.936 (1.00) |
|
MIRseq Mature CNMF subtypes |
5.18e-05 (0.00384) |
0.218 (1.00) |
0.00399 (0.228) |
0.00131 (0.0814) |
0.166 (1.00) |
0.0443 (1.00) |
0.00419 (0.235) |
0.47 (1.00) |
|
MIRseq Mature cHierClus subtypes |
0.0466 (1.00) |
0.634 (1.00) |
0.148 (1.00) |
0.151 (1.00) |
0.154 (1.00) |
0.178 (1.00) |
0.0124 (0.558) |
0.33 (1.00) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 34 | 24 | 14 |
P value = 0.0843 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 71 | 14 | 0.5 - 101.1 (36.8) |
subtype1 | 33 | 4 | 11.1 - 101.1 (32.6) |
subtype2 | 24 | 9 | 0.5 - 93.3 (38.2) |
subtype3 | 14 | 1 | 10.5 - 84.4 (38.8) |
P value = 0.791 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 71 | 60.5 (12.4) |
subtype1 | 33 | 60.2 (13.8) |
subtype2 | 24 | 59.9 (11.1) |
subtype3 | 14 | 62.6 (11.3) |
P value = 0.00527 (Chi-square test), Q value = 0.29
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 40 | 13 | 14 | 5 |
subtype1 | 23 | 4 | 6 | 1 |
subtype2 | 9 | 3 | 8 | 4 |
subtype3 | 8 | 6 | 0 | 0 |
P value = 0.00911 (Chi-square test), Q value = 0.46
nPatients | T1 | T2 | T3 |
---|---|---|---|
ALL | 41 | 14 | 17 |
subtype1 | 23 | 4 | 7 |
subtype2 | 10 | 4 | 10 |
subtype3 | 8 | 6 | 0 |
P value = 0.0704 (Fisher's exact test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 35 | 3 |
subtype1 | 18 | 0 |
subtype2 | 10 | 3 |
subtype3 | 7 | 0 |
P value = 0.12 (Fisher's exact test), Q value = 1
nPatients | M0 | M1 |
---|---|---|
ALL | 67 | 5 |
subtype1 | 33 | 1 |
subtype2 | 20 | 4 |
subtype3 | 14 | 0 |
P value = 0.634 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 29 | 43 |
subtype1 | 15 | 19 |
subtype2 | 10 | 14 |
subtype3 | 4 | 10 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 15 | 33 | 24 |
P value = 0.313 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 71 | 14 | 0.5 - 101.1 (36.8) |
subtype1 | 15 | 2 | 1.7 - 84.4 (37.6) |
subtype2 | 32 | 4 | 11.1 - 101.1 (32.0) |
subtype3 | 24 | 8 | 0.5 - 93.3 (38.2) |
P value = 0.647 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 71 | 60.5 (12.4) |
subtype1 | 15 | 63.2 (11.2) |
subtype2 | 32 | 59.9 (14.0) |
subtype3 | 24 | 59.7 (10.9) |
P value = 0.00728 (Chi-square test), Q value = 0.39
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 40 | 13 | 14 | 5 |
subtype1 | 9 | 6 | 0 | 0 |
subtype2 | 22 | 4 | 6 | 1 |
subtype3 | 9 | 3 | 8 | 4 |
P value = 0.0125 (Chi-square test), Q value = 0.56
nPatients | T1 | T2 | T3 |
---|---|---|---|
ALL | 41 | 14 | 17 |
subtype1 | 9 | 6 | 0 |
subtype2 | 22 | 4 | 7 |
subtype3 | 10 | 4 | 10 |
P value = 0.124 (Fisher's exact test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 35 | 3 |
subtype1 | 7 | 0 |
subtype2 | 17 | 0 |
subtype3 | 11 | 3 |
P value = 0.104 (Fisher's exact test), Q value = 1
nPatients | M0 | M1 |
---|---|---|
ALL | 67 | 5 |
subtype1 | 15 | 0 |
subtype2 | 32 | 1 |
subtype3 | 20 | 4 |
P value = 0.68 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 29 | 43 |
subtype1 | 5 | 10 |
subtype2 | 15 | 18 |
subtype3 | 9 | 15 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 158 | 214 | 126 |
P value = 0.00014 (logrank test), Q value = 0.0099
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 497 | 166 | 0.1 - 120.6 (37.1) |
subtype1 | 158 | 71 | 0.2 - 109.9 (33.5) |
subtype2 | 213 | 49 | 0.2 - 120.6 (37.2) |
subtype3 | 126 | 46 | 0.1 - 103.4 (37.4) |
P value = 0.00193 (ANOVA), Q value = 0.12
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 497 | 60.5 (12.2) |
subtype1 | 157 | 63.3 (11.5) |
subtype2 | 214 | 59.6 (12.5) |
subtype3 | 126 | 58.7 (11.9) |
P value = 3.98e-05 (Chi-square test), Q value = 0.003
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 239 | 55 | 125 | 79 |
subtype1 | 57 | 17 | 45 | 39 |
subtype2 | 125 | 27 | 45 | 17 |
subtype3 | 57 | 11 | 35 | 23 |
P value = 0.000984 (Chi-square test), Q value = 0.064
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 245 | 66 | 176 | 11 |
subtype1 | 62 | 21 | 71 | 4 |
subtype2 | 125 | 30 | 58 | 1 |
subtype3 | 58 | 15 | 47 | 6 |
P value = 0.0121 (Fisher's exact test), Q value = 0.56
nPatients | 0 | 1 |
---|---|---|
ALL | 229 | 18 |
subtype1 | 69 | 9 |
subtype2 | 103 | 2 |
subtype3 | 57 | 7 |
P value = 0.000656 (Chi-square test), Q value = 0.045
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 417 | 78 | 3 |
subtype1 | 118 | 39 | 1 |
subtype2 | 194 | 18 | 2 |
subtype3 | 105 | 21 | 0 |
P value = 0.00833 (Fisher's exact test), Q value = 0.43
nPatients | FEMALE | MALE |
---|---|---|
ALL | 173 | 325 |
subtype1 | 40 | 118 |
subtype2 | 86 | 128 |
subtype3 | 47 | 79 |
P value = 0.722 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 36 | 88.3 (23.0) |
subtype1 | 14 | 87.1 (25.8) |
subtype2 | 13 | 92.3 (9.3) |
subtype3 | 9 | 84.4 (32.4) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 97 | 114 | 74 |
P value = 5.35e-06 (logrank test), Q value = 0.00042
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 285 | 97 | 0.2 - 120.6 (31.3) |
subtype1 | 97 | 49 | 0.6 - 90.1 (28.9) |
subtype2 | 114 | 20 | 0.2 - 120.6 (37.2) |
subtype3 | 74 | 28 | 0.2 - 109.9 (28.5) |
P value = 0.0063 (ANOVA), Q value = 0.34
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 285 | 61.5 (11.9) |
subtype1 | 97 | 63.9 (10.5) |
subtype2 | 114 | 58.9 (12.8) |
subtype3 | 74 | 62.5 (11.7) |
P value = 1.47e-11 (Chi-square test), Q value = 1.3e-09
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 130 | 28 | 73 | 54 |
subtype1 | 19 | 9 | 39 | 30 |
subtype2 | 76 | 16 | 12 | 10 |
subtype3 | 35 | 3 | 22 | 14 |
P value = 5.4e-12 (Chi-square test), Q value = 5e-10
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 133 | 37 | 107 | 8 |
subtype1 | 20 | 14 | 60 | 3 |
subtype2 | 76 | 19 | 19 | 0 |
subtype3 | 37 | 4 | 28 | 5 |
P value = 0.169 (Fisher's exact test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 127 | 9 |
subtype1 | 41 | 5 |
subtype2 | 51 | 1 |
subtype3 | 35 | 3 |
P value = 0.000759 (Fisher's exact test), Q value = 0.051
nPatients | M0 | M1 |
---|---|---|
ALL | 233 | 52 |
subtype1 | 68 | 29 |
subtype2 | 103 | 11 |
subtype3 | 62 | 12 |
P value = 0.000957 (Fisher's exact test), Q value = 0.063
nPatients | FEMALE | MALE |
---|---|---|
ALL | 96 | 189 |
subtype1 | 20 | 77 |
subtype2 | 51 | 63 |
subtype3 | 25 | 49 |
P value = 0.595 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 28 | 92.5 (8.0) |
subtype1 | 6 | 91.7 (7.5) |
subtype2 | 14 | 91.4 (8.6) |
subtype3 | 8 | 95.0 (7.6) |
Cluster Labels | 1 | 2 | 3 | 4 | 5 | 6 |
---|---|---|---|---|---|---|
Number of samples | 101 | 90 | 86 | 76 | 44 | 57 |
P value = 1.71e-10 (logrank test), Q value = 1.5e-08
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 454 | 158 | 0.1 - 120.6 (37.0) |
subtype1 | 101 | 27 | 0.2 - 120.6 (47.1) |
subtype2 | 90 | 36 | 0.1 - 105.9 (32.4) |
subtype3 | 86 | 24 | 0.2 - 112.8 (35.9) |
subtype4 | 76 | 24 | 0.8 - 99.8 (37.2) |
subtype5 | 44 | 8 | 0.9 - 103.4 (37.1) |
subtype6 | 57 | 39 | 0.6 - 102.5 (22.3) |
P value = 0.13 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 453 | 60.4 (12.3) |
subtype1 | 100 | 61.8 (11.1) |
subtype2 | 90 | 58.0 (12.2) |
subtype3 | 86 | 62.3 (12.6) |
subtype4 | 76 | 60.0 (11.8) |
subtype5 | 44 | 58.3 (15.7) |
subtype6 | 57 | 61.2 (11.4) |
P value = 1.2e-08 (Chi-square test), Q value = 1.1e-06
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 219 | 44 | 115 | 76 |
subtype1 | 54 | 11 | 22 | 14 |
subtype2 | 42 | 9 | 19 | 20 |
subtype3 | 39 | 12 | 24 | 11 |
subtype4 | 44 | 7 | 17 | 8 |
subtype5 | 33 | 2 | 9 | 0 |
subtype6 | 7 | 3 | 24 | 23 |
P value = 9.52e-07 (Chi-square test), Q value = 7.8e-05
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 224 | 54 | 165 | 11 |
subtype1 | 54 | 15 | 31 | 1 |
subtype2 | 43 | 10 | 32 | 5 |
subtype3 | 41 | 12 | 33 | 0 |
subtype4 | 45 | 7 | 23 | 1 |
subtype5 | 33 | 2 | 9 | 0 |
subtype6 | 8 | 8 | 37 | 4 |
P value = 0.00106 (Chi-square test), Q value = 0.068
nPatients | 0 | 1 |
---|---|---|
ALL | 208 | 16 |
subtype1 | 48 | 1 |
subtype2 | 46 | 2 |
subtype3 | 43 | 2 |
subtype4 | 35 | 3 |
subtype5 | 13 | 0 |
subtype6 | 23 | 8 |
P value = 6.21e-07 (Chi-square test), Q value = 5.2e-05
nPatients | M0 | M1 |
---|---|---|
ALL | 379 | 75 |
subtype1 | 87 | 14 |
subtype2 | 71 | 19 |
subtype3 | 75 | 11 |
subtype4 | 68 | 8 |
subtype5 | 44 | 0 |
subtype6 | 34 | 23 |
P value = 0.0611 (Chi-square test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 151 | 303 |
subtype1 | 44 | 57 |
subtype2 | 29 | 61 |
subtype3 | 27 | 59 |
subtype4 | 18 | 58 |
subtype5 | 18 | 26 |
subtype6 | 15 | 42 |
P value = 0.322 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 34 | 93.5 (7.7) |
subtype1 | 7 | 92.9 (7.6) |
subtype2 | 8 | 93.8 (5.2) |
subtype3 | 10 | 90.0 (9.4) |
subtype4 | 2 | 100.0 (0.0) |
subtype5 | 4 | 100.0 (0.0) |
subtype6 | 3 | 93.3 (11.5) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 189 | 153 | 112 |
P value = 1.02e-07 (logrank test), Q value = 8.9e-06
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 454 | 158 | 0.1 - 120.6 (37.0) |
subtype1 | 189 | 44 | 0.5 - 105.9 (37.5) |
subtype2 | 153 | 52 | 0.2 - 120.6 (39.5) |
subtype3 | 112 | 62 | 0.1 - 112.8 (27.1) |
P value = 0.00939 (ANOVA), Q value = 0.47
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 453 | 60.4 (12.3) |
subtype1 | 189 | 58.4 (12.7) |
subtype2 | 152 | 62.3 (12.3) |
subtype3 | 112 | 61.3 (11.1) |
P value = 2e-06 (Chi-square test), Q value = 0.00016
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 219 | 44 | 115 | 76 |
subtype1 | 118 | 16 | 36 | 19 |
subtype2 | 65 | 17 | 47 | 24 |
subtype3 | 36 | 11 | 32 | 33 |
P value = 4.03e-07 (Chi-square test), Q value = 3.4e-05
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 224 | 54 | 165 | 11 |
subtype1 | 120 | 19 | 48 | 2 |
subtype2 | 67 | 20 | 65 | 1 |
subtype3 | 37 | 15 | 52 | 8 |
P value = 0.241 (Fisher's exact test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 208 | 16 |
subtype1 | 72 | 4 |
subtype2 | 76 | 4 |
subtype3 | 60 | 8 |
P value = 0.000108 (Fisher's exact test), Q value = 0.0079
nPatients | M0 | M1 |
---|---|---|
ALL | 379 | 75 |
subtype1 | 170 | 19 |
subtype2 | 130 | 23 |
subtype3 | 79 | 33 |
P value = 0.571 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 151 | 303 |
subtype1 | 68 | 121 |
subtype2 | 47 | 106 |
subtype3 | 36 | 76 |
P value = 0.0463 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 34 | 93.5 (7.7) |
subtype1 | 17 | 96.5 (4.9) |
subtype2 | 10 | 89.0 (8.8) |
subtype3 | 7 | 92.9 (9.5) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 211 | 108 | 184 |
P value = 3.31e-07 (logrank test), Q value = 2.9e-05
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 502 | 166 | 0.1 - 120.6 (37.1) |
subtype1 | 210 | 46 | 0.2 - 120.6 (39.3) |
subtype2 | 108 | 33 | 0.1 - 112.8 (37.9) |
subtype3 | 184 | 87 | 0.2 - 109.9 (30.9) |
P value = 0.193 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 502 | 60.6 (12.2) |
subtype1 | 210 | 61.4 (12.2) |
subtype2 | 108 | 58.8 (13.0) |
subtype3 | 184 | 60.8 (11.8) |
P value = 3.81e-07 (Chi-square test), Q value = 3.2e-05
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 244 | 55 | 124 | 80 |
subtype1 | 125 | 22 | 41 | 23 |
subtype2 | 62 | 12 | 22 | 12 |
subtype3 | 57 | 21 | 61 | 45 |
P value = 1.46e-05 (Chi-square test), Q value = 0.0011
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 250 | 66 | 176 | 11 |
subtype1 | 125 | 25 | 59 | 2 |
subtype2 | 63 | 13 | 29 | 3 |
subtype3 | 62 | 28 | 88 | 6 |
P value = 0.0112 (Fisher's exact test), Q value = 0.54
nPatients | 0 | 1 |
---|---|---|
ALL | 235 | 17 |
subtype1 | 100 | 2 |
subtype2 | 51 | 3 |
subtype3 | 84 | 12 |
P value = 0.00129 (Chi-square test), Q value = 0.081
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 421 | 79 | 3 |
subtype1 | 186 | 23 | 2 |
subtype2 | 97 | 11 | 0 |
subtype3 | 138 | 45 | 1 |
P value = 4.81e-05 (Fisher's exact test), Q value = 0.0036
nPatients | FEMALE | MALE |
---|---|---|
ALL | 172 | 331 |
subtype1 | 94 | 117 |
subtype2 | 35 | 73 |
subtype3 | 43 | 141 |
P value = 0.656 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 35 | 90.9 (17.6) |
subtype1 | 14 | 91.4 (8.6) |
subtype2 | 8 | 95.0 (7.6) |
subtype3 | 13 | 87.7 (27.1) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 221 | 201 | 81 |
P value = 3.05e-11 (logrank test), Q value = 2.7e-09
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 502 | 166 | 0.1 - 120.6 (37.1) |
subtype1 | 220 | 46 | 0.2 - 120.6 (39.5) |
subtype2 | 201 | 102 | 0.1 - 109.9 (29.1) |
subtype3 | 81 | 18 | 0.5 - 112.8 (43.0) |
P value = 0.256 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 502 | 60.6 (12.2) |
subtype1 | 220 | 60.9 (12.5) |
subtype2 | 201 | 61.2 (11.6) |
subtype3 | 81 | 58.6 (13.0) |
P value = 2.65e-13 (Chi-square test), Q value = 2.5e-11
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 244 | 55 | 124 | 80 |
subtype1 | 129 | 25 | 46 | 21 |
subtype2 | 57 | 21 | 69 | 54 |
subtype3 | 58 | 9 | 9 | 5 |
P value = 2.3e-12 (Chi-square test), Q value = 2.1e-10
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 250 | 66 | 176 | 11 |
subtype1 | 129 | 29 | 63 | 0 |
subtype2 | 62 | 27 | 102 | 10 |
subtype3 | 59 | 10 | 11 | 1 |
P value = 0.00202 (Fisher's exact test), Q value = 0.12
nPatients | 0 | 1 |
---|---|---|
ALL | 235 | 17 |
subtype1 | 102 | 1 |
subtype2 | 99 | 14 |
subtype3 | 34 | 2 |
P value = 1.47e-05 (Chi-square test), Q value = 0.0011
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 421 | 79 | 3 |
subtype1 | 197 | 22 | 2 |
subtype2 | 148 | 52 | 1 |
subtype3 | 76 | 5 | 0 |
P value = 0.000495 (Fisher's exact test), Q value = 0.034
nPatients | FEMALE | MALE |
---|---|---|
ALL | 172 | 331 |
subtype1 | 96 | 125 |
subtype2 | 52 | 149 |
subtype3 | 24 | 57 |
P value = 0.35 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 35 | 90.9 (17.6) |
subtype1 | 14 | 92.9 (6.1) |
subtype2 | 11 | 84.5 (29.1) |
subtype3 | 10 | 95.0 (9.7) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 109 | 206 | 171 |
P value = 3.45e-06 (logrank test), Q value = 0.00028
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 486 | 164 | 0.1 - 120.6 (37.1) |
subtype1 | 109 | 32 | 0.1 - 112.8 (45.1) |
subtype2 | 206 | 49 | 0.2 - 120.6 (37.1) |
subtype3 | 171 | 83 | 0.2 - 94.0 (33.5) |
P value = 0.111 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 486 | 60.6 (12.1) |
subtype1 | 109 | 59.1 (11.8) |
subtype2 | 206 | 61.9 (12.6) |
subtype3 | 171 | 59.9 (11.7) |
P value = 0.000111 (Chi-square test), Q value = 0.008
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 230 | 53 | 124 | 79 |
subtype1 | 61 | 9 | 29 | 10 |
subtype2 | 113 | 21 | 45 | 27 |
subtype3 | 56 | 23 | 50 | 42 |
P value = 0.000458 (Chi-square test), Q value = 0.032
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 236 | 64 | 175 | 11 |
subtype1 | 63 | 9 | 33 | 4 |
subtype2 | 114 | 26 | 64 | 2 |
subtype3 | 59 | 29 | 78 | 5 |
P value = 0.0113 (Fisher's exact test), Q value = 0.54
nPatients | 0 | 1 |
---|---|---|
ALL | 223 | 18 |
subtype1 | 54 | 3 |
subtype2 | 87 | 2 |
subtype3 | 82 | 13 |
P value = 0.0023 (Chi-square test), Q value = 0.13
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 405 | 78 | 3 |
subtype1 | 100 | 9 | 0 |
subtype2 | 177 | 27 | 2 |
subtype3 | 128 | 42 | 1 |
P value = 0.00203 (Fisher's exact test), Q value = 0.12
nPatients | FEMALE | MALE |
---|---|---|
ALL | 165 | 321 |
subtype1 | 37 | 72 |
subtype2 | 86 | 120 |
subtype3 | 42 | 129 |
P value = 0.155 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 36 | 88.3 (23.0) |
subtype1 | 8 | 95.0 (7.6) |
subtype2 | 17 | 92.4 (8.3) |
subtype3 | 11 | 77.3 (38.8) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 44 | 263 | 179 |
P value = 0.0105 (logrank test), Q value = 0.51
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 486 | 164 | 0.1 - 120.6 (37.1) |
subtype1 | 44 | 17 | 1.7 - 93.3 (40.4) |
subtype2 | 263 | 73 | 0.2 - 120.6 (37.0) |
subtype3 | 179 | 74 | 0.1 - 112.8 (37.0) |
P value = 0.0234 (ANOVA), Q value = 0.98
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 486 | 60.6 (12.1) |
subtype1 | 44 | 57.8 (11.1) |
subtype2 | 263 | 61.9 (12.1) |
subtype3 | 179 | 59.3 (12.3) |
P value = 0.127 (Chi-square test), Q value = 1
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 230 | 53 | 124 | 79 |
subtype1 | 17 | 9 | 8 | 10 |
subtype2 | 131 | 29 | 62 | 41 |
subtype3 | 82 | 15 | 54 | 28 |
P value = 0.0236 (Chi-square test), Q value = 0.98
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 236 | 64 | 175 | 11 |
subtype1 | 18 | 11 | 13 | 2 |
subtype2 | 134 | 36 | 91 | 2 |
subtype3 | 84 | 17 | 71 | 7 |
P value = 0.0134 (Fisher's exact test), Q value = 0.58
nPatients | 0 | 1 |
---|---|---|
ALL | 223 | 18 |
subtype1 | 20 | 4 |
subtype2 | 122 | 4 |
subtype3 | 81 | 10 |
P value = 0.745 (Chi-square test), Q value = 1
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 405 | 78 | 3 |
subtype1 | 34 | 10 | 0 |
subtype2 | 220 | 41 | 2 |
subtype3 | 151 | 27 | 1 |
P value = 0.363 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 165 | 321 |
subtype1 | 17 | 27 |
subtype2 | 94 | 169 |
subtype3 | 54 | 125 |
P value = 0.936 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 36 | 88.3 (23.0) |
subtype1 | 1 | 90.0 (NA) |
subtype2 | 20 | 88.0 (22.4) |
subtype3 | 15 | 88.7 (25.3) |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 45 | 96 | 83 |
P value = 5.18e-05 (logrank test), Q value = 0.0038
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 224 | 69 | 0.2 - 120.6 (38.5) |
subtype1 | 45 | 11 | 3.9 - 112.8 (36.5) |
subtype2 | 96 | 18 | 0.2 - 120.6 (45.1) |
subtype3 | 83 | 40 | 0.2 - 93.0 (36.2) |
P value = 0.218 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 224 | 59.4 (12.6) |
subtype1 | 45 | 57.1 (11.6) |
subtype2 | 96 | 60.9 (12.3) |
subtype3 | 83 | 59.0 (13.4) |
P value = 0.00399 (Chi-square test), Q value = 0.23
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 108 | 26 | 47 | 43 |
subtype1 | 31 | 2 | 5 | 7 |
subtype2 | 48 | 14 | 22 | 12 |
subtype3 | 29 | 10 | 20 | 24 |
P value = 0.00131 (Chi-square test), Q value = 0.081
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 112 | 29 | 77 | 6 |
subtype1 | 32 | 2 | 8 | 3 |
subtype2 | 48 | 17 | 30 | 1 |
subtype3 | 32 | 10 | 39 | 2 |
P value = 0.166 (Fisher's exact test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 97 | 8 |
subtype1 | 15 | 2 |
subtype2 | 43 | 1 |
subtype3 | 39 | 5 |
P value = 0.0443 (Chi-square test), Q value = 1
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 181 | 40 | 3 |
subtype1 | 39 | 6 | 0 |
subtype2 | 83 | 11 | 2 |
subtype3 | 59 | 23 | 1 |
P value = 0.00419 (Fisher's exact test), Q value = 0.23
nPatients | FEMALE | MALE |
---|---|---|
ALL | 67 | 157 |
subtype1 | 13 | 32 |
subtype2 | 39 | 57 |
subtype3 | 15 | 68 |
P value = 0.47 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 30 | 93.3 (8.0) |
subtype1 | 7 | 95.7 (7.9) |
subtype2 | 14 | 91.4 (8.6) |
subtype3 | 9 | 94.4 (7.3) |
Cluster Labels | 1 | 2 | 3 | 4 | 5 |
---|---|---|---|---|---|
Number of samples | 10 | 49 | 77 | 10 | 78 |
P value = 0.0466 (logrank test), Q value = 1
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 224 | 69 | 0.2 - 120.6 (38.5) |
subtype1 | 10 | 3 | 5.8 - 68.7 (31.4) |
subtype2 | 49 | 17 | 0.5 - 112.8 (35.2) |
subtype3 | 77 | 31 | 0.2 - 93.0 (37.1) |
subtype4 | 10 | 3 | 6.7 - 87.5 (36.9) |
subtype5 | 78 | 15 | 0.8 - 120.6 (46.6) |
P value = 0.634 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 224 | 59.4 (12.6) |
subtype1 | 10 | 58.1 (10.9) |
subtype2 | 49 | 57.5 (13.3) |
subtype3 | 77 | 59.7 (12.4) |
subtype4 | 10 | 57.4 (13.9) |
subtype5 | 78 | 60.9 (12.6) |
P value = 0.148 (Chi-square test), Q value = 1
nPatients | STAGE I | STAGE II | STAGE III | STAGE IV |
---|---|---|---|---|
ALL | 108 | 26 | 47 | 43 |
subtype1 | 8 | 1 | 0 | 1 |
subtype2 | 23 | 6 | 11 | 9 |
subtype3 | 30 | 10 | 14 | 23 |
subtype4 | 7 | 0 | 2 | 1 |
subtype5 | 40 | 9 | 20 | 9 |
P value = 0.151 (Chi-square test), Q value = 1
nPatients | T1 | T2 | T3 | T4 |
---|---|---|---|---|
ALL | 112 | 29 | 77 | 6 |
subtype1 | 8 | 1 | 1 | 0 |
subtype2 | 24 | 6 | 15 | 4 |
subtype3 | 31 | 12 | 33 | 1 |
subtype4 | 7 | 0 | 3 | 0 |
subtype5 | 42 | 10 | 25 | 1 |
P value = 0.154 (Chi-square test), Q value = 1
nPatients | 0 | 1 |
---|---|---|
ALL | 97 | 8 |
subtype1 | 3 | 0 |
subtype2 | 22 | 5 |
subtype3 | 39 | 1 |
subtype4 | 4 | 0 |
subtype5 | 29 | 2 |
P value = 0.178 (Chi-square test), Q value = 1
nPatients | M0 | M1 | MX |
---|---|---|---|
ALL | 181 | 40 | 3 |
subtype1 | 9 | 1 | 0 |
subtype2 | 41 | 7 | 1 |
subtype3 | 55 | 22 | 0 |
subtype4 | 9 | 1 | 0 |
subtype5 | 67 | 9 | 2 |
P value = 0.0124 (Chi-square test), Q value = 0.56
nPatients | FEMALE | MALE |
---|---|---|
ALL | 67 | 157 |
subtype1 | 4 | 6 |
subtype2 | 11 | 38 |
subtype3 | 19 | 58 |
subtype4 | 0 | 10 |
subtype5 | 33 | 45 |
P value = 0.33 (ANOVA), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 30 | 93.3 (8.0) |
subtype1 | 3 | 96.7 (5.8) |
subtype2 | 6 | 93.3 (8.2) |
subtype3 | 11 | 90.0 (10.0) |
subtype5 | 10 | 96.0 (5.2) |
-
Cluster data file = KIRC-TP.mergedcluster.txt
-
Clinical data file = KIRC-TP.merged_data.txt
-
Number of patients = 507
-
Number of clustering approaches = 12
-
Number of selected clinical features = 9
-
Exclude small clusters that include fewer than K patients, K = 3
consensus non-negative matrix factorization clustering approach (Brunet et al. 2004)
Resampling-based clustering method (Monti et al. 2003)
For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R
For continuous numerical clinical features, one-way analysis of variance (Howell 2002) was applied to compare the clinical values between tumor subtypes using 'anova' function in R
For multi-class clinical features (nominal or ordinal), Chi-square tests (Greenwood and Nikulin 1996) were used to estimate the P values using the 'chisq.test' function in R
For binary clinical features, two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.