This pipeline computes the correlation between significantly recurrent gene mutations and selected clinical features.
Testing the association between mutation status of 7 genes and 10 clinical features across 155 patients, no significant finding detected with Q value < 0.25.
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No gene mutations related to clinical features.
Clinical Features |
Time to Death |
AGE |
NEOPLASM DISEASESTAGE |
PATHOLOGY T STAGE |
PATHOLOGY N STAGE |
PATHOLOGY M STAGE |
GENDER |
KARNOFSKY PERFORMANCE SCORE |
NUMBERPACKYEARSSMOKED | YEAROFTOBACCOSMOKINGONSET | ||
nMutated (%) | nWild-Type | logrank test | t-test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | t-test | t-test | t-test | |
SKI | 6 (4%) | 149 |
0.657 (1.00) |
0.714 (1.00) |
0.739 (1.00) |
0.678 (1.00) |
1 (1.00) |
0.668 (1.00) |
||||
NEFH | 9 (6%) | 146 |
0.397 (1.00) |
0.427 (1.00) |
0.262 (1.00) |
0.0671 (1.00) |
0.196 (1.00) |
1 (1.00) |
||||
HNRNPM | 11 (7%) | 144 |
0.192 (1.00) |
0.307 (1.00) |
0.853 (1.00) |
0.718 (1.00) |
0.838 (1.00) |
0.506 (1.00) |
0.289 (1.00) |
|||
NF2 | 11 (7%) | 144 |
0.174 (1.00) |
0.332 (1.00) |
0.04 (1.00) |
0.0984 (1.00) |
0.0166 (0.813) |
0.108 (1.00) |
0.31 (1.00) |
|||
ZNF598 | 12 (8%) | 143 |
0.336 (1.00) |
0.302 (1.00) |
0.358 (1.00) |
0.488 (1.00) |
0.598 (1.00) |
0.126 (1.00) |
0.755 (1.00) |
0.604 (1.00) |
||
BMS1 | 13 (8%) | 142 |
0.129 (1.00) |
0.211 (1.00) |
0.853 (1.00) |
0.0902 (1.00) |
0.198 (1.00) |
0.755 (1.00) |
0.504 (1.00) |
|||
MUC2 | 25 (16%) | 130 |
0.0641 (1.00) |
0.144 (1.00) |
0.182 (1.00) |
0.823 (1.00) |
0.361 (1.00) |
0.131 (1.00) |
0.635 (1.00) |
0.025 (1.00) |
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Mutation data file = transformed.cor.cli.txt
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Clinical data file = KIRP-TP.merged_data.txt
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Number of patients = 155
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Number of significantly mutated genes = 7
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Number of selected clinical features = 10
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Exclude genes that fewer than K tumors have mutations, K = 3
For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R
For continuous numerical clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the clinical values between tumors with and without gene mutations using 't.test' function in R
For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.