This pipeline uses various statistical tests to identify selected clinical features related to mutation rate.
Testing the association between 2 variables and 11 clinical features across 66 samples, statistically thresholded by P value < 0.05 and Q value < 0.3, 6 clinical features related to at least one variables.
-
2 variables correlated to 'AGE'.
-
MUTATIONRATE_NONSYNONYMOUS , MUTATIONRATE_SILENT
-
1 variable correlated to 'AGE_mutation.rate'.
-
MUTATIONRATE_NONSYNONYMOUS
-
2 variables correlated to 'NEOPLASM.DISEASESTAGE'.
-
MUTATIONRATE_NONSYNONYMOUS , MUTATIONRATE_SILENT
-
2 variables correlated to 'PATHOLOGY.T.STAGE'.
-
MUTATIONRATE_SILENT , MUTATIONRATE_NONSYNONYMOUS
-
2 variables correlated to 'PATHOLOGY.N.STAGE'.
-
MUTATIONRATE_NONSYNONYMOUS , MUTATIONRATE_SILENT
-
1 variable correlated to 'ETHNICITY'.
-
MUTATIONRATE_NONSYNONYMOUS
-
No variables correlated to 'PATHOLOGY.M.STAGE', 'GENDER', 'KARNOFSKY.PERFORMANCE.SCORE', 'NUMBERPACKYEARSSMOKED', and 'RACE'.
Complete statistical result table is provided in Supplement Table 1
Clinical feature | Statistical test | Significant variables | Associated with | Associated with | ||
---|---|---|---|---|---|---|
AGE | Spearman correlation test | N=2 | older | N=2 | younger | N=0 |
AGE | Linear Regression Analysis | N=1 | ||||
NEOPLASM DISEASESTAGE | Kruskal-Wallis test | N=2 | ||||
PATHOLOGY T STAGE | Spearman correlation test | N=2 | higher stage | N=2 | lower stage | N=0 |
PATHOLOGY N STAGE | Spearman correlation test | N=2 | higher stage | N=2 | lower stage | N=0 |
PATHOLOGY M STAGE | Kruskal-Wallis test | N=0 | ||||
GENDER | Wilcoxon test | N=0 | ||||
KARNOFSKY PERFORMANCE SCORE | Spearman correlation test | N=0 | ||||
NUMBERPACKYEARSSMOKED | Spearman correlation test | N=0 | ||||
RACE | Kruskal-Wallis test | N=0 | ||||
ETHNICITY | Wilcoxon test | N=1 | not hispanic or latino | N=1 | hispanic or latino | N=0 |
AGE | Mean (SD) | 51.52 (14) |
Significant variables | N = 2 | |
pos. correlated | 2 | |
neg. correlated | 0 |
AGE | Mean (SD) | 51.52 (14) |
Significant variables | N = 1 |
Adj.R.squared | F | P | Residual.std.err | DF | coef(intercept) | coef.p(intercept) | |
---|---|---|---|---|---|---|---|
MUTATIONRATE_NONSYNONYMOUS | 0.0443 | 4.01 | 0.0494 | 2.47e-06 | 64 | 4.3e-08 ( -4.64e-07 ) | 0.0494 ( 0.687 ) |
NEOPLASM.DISEASESTAGE | Labels | N |
STAGE I | 21 | |
STAGE II | 25 | |
STAGE III | 14 | |
STAGE IV | 6 | |
Significant variables | N = 2 |
PATHOLOGY.T.STAGE | Mean (SD) | 2.02 (0.85) |
N | ||
1 | 21 | |
2 | 25 | |
3 | 18 | |
4 | 2 | |
Significant variables | N = 2 | |
pos. correlated | 2 | |
neg. correlated | 0 |
PATHOLOGY.N.STAGE | Mean (SD) | 0.16 (0.47) |
N | ||
0 | 40 | |
1 | 3 | |
2 | 2 | |
Significant variables | N = 2 | |
pos. correlated | 2 | |
neg. correlated | 0 |
PATHOLOGY.M.STAGE | Labels | N |
M0 | 34 | |
M1 | 2 | |
MX | 9 | |
Significant variables | N = 0 |
GENDER | Labels | N |
FEMALE | 27 | |
MALE | 39 | |
Significant variables | N = 0 |
No variable related to 'KARNOFSKY.PERFORMANCE.SCORE'.
KARNOFSKY.PERFORMANCE.SCORE | Mean (SD) | 89.09 (9.4) |
Score | N | |
70 | 1 | |
80 | 2 | |
90 | 5 | |
100 | 3 | |
Significant variables | N = 0 |
NUMBERPACKYEARSSMOKED | Mean (SD) | 25.09 (22) |
Significant variables | N = 0 |
RACE | Labels | N |
ASIAN | 2 | |
BLACK OR AFRICAN AMERICAN | 4 | |
WHITE | 58 | |
Significant variables | N = 0 |
ETHNICITY | Labels | N |
HISPANIC OR LATINO | 4 | |
NOT HISPANIC OR LATINO | 32 | |
Significant variables | N = 1 | |
Higher in NOT HISPANIC OR LATINO | 1 | |
Higher in HISPANIC OR LATINO | 0 |
W(pos if higher in 'NOT HISPANIC OR LATINO') | wilcoxontestP | Q | AUC | |
---|---|---|---|---|
MUTATIONRATE_NONSYNONYMOUS | c("105", "0.04149") | c("105", "0.04149") | 0.083 | 0.8203 |
-
Expresson data file = KICH-TP.patients.counts_and_rates.txt
-
Clinical data file = KICH-TP.merged_data.txt
-
Number of patients = 66
-
Number of variables = 2
-
Number of clinical features = 11
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.