This pipeline uses various statistical tests to identify RPPAs whose expression levels correlated to selected clinical features.
Testing the association between 189 genes and 8 clinical features across 254 samples, statistically thresholded by P value < 0.05 and Q value < 0.3, 5 clinical features related to at least one genes.
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23 genes correlated to 'Time to Death'.
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ANXA1|ANNEXIN-1-M-E , MAPK14|P38_MAPK-R-V , SERPINE1|PAI-1-M-E , PGR|PR-R-V , CHEK2|CHK2_PT68-R-E , ...
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8 genes correlated to 'AGE'.
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IGFBP2|IGFBP2-R-V , PEA15|PEA15-R-V , SERPINE1|PAI-1-M-E , EGFR|EGFR-R-V , ASNS|ASNS-R-V , ...
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3 genes correlated to 'GENDER'.
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STAT5A|STAT5-ALPHA-R-V , RAB11A RAB11B|RAB11-R-E , TSC1|TSC1-R-C
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1 gene correlated to 'KARNOFSKY.PERFORMANCE.SCORE'.
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IGFBP2|IGFBP2-R-V
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46 genes correlated to 'HISTOLOGICAL.TYPE'.
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SYK|SYK-M-V , ANXA1|ANNEXIN-1-M-E , AR|AR-R-V , BAX|BAX-R-V , MAPK14|P38_MAPK-R-V , ...
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No genes correlated to 'RADIATIONS.RADIATION.REGIMENINDICATION', 'RACE', and 'ETHNICITY'.
Complete statistical result table is provided in Supplement Table 1
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
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Time to Death | Cox regression test | N=23 | shorter survival | N=11 | longer survival | N=12 |
AGE | Spearman correlation test | N=8 | older | N=6 | younger | N=2 |
GENDER | Wilcoxon test | N=3 | male | N=3 | female | N=0 |
KARNOFSKY PERFORMANCE SCORE | Spearman correlation test | N=1 | higher score | N=0 | lower score | N=1 |
HISTOLOGICAL TYPE | Kruskal-Wallis test | N=46 | ||||
RADIATIONS RADIATION REGIMENINDICATION | Wilcoxon test | N=0 | ||||
RACE | Wilcoxon test | N=0 | ||||
ETHNICITY | Wilcoxon test | N=0 |
Time to Death | Duration (Months) | 0-211.2 (median=16.1) |
censored | N = 197 | |
death | N = 55 | |
Significant markers | N = 23 | |
associated with shorter survival | 11 | |
associated with longer survival | 12 |
HazardRatio | Wald_P | Q | C_index | |
---|---|---|---|---|
ANXA1|ANNEXIN-1-M-E | 2.1 | 2.035e-07 | 3.8e-05 | 0.721 |
MAPK14|P38_MAPK-R-V | 3.7 | 1.425e-05 | 0.0027 | 0.69 |
SERPINE1|PAI-1-M-E | 2.3 | 1.56e-05 | 0.0029 | 0.68 |
PGR|PR-R-V | 0 | 4.765e-05 | 0.0089 | 0.3 |
CHEK2|CHK2_PT68-R-E | 0.03 | 0.0001042 | 0.019 | 0.318 |
SMAD1|SMAD1-R-V | 5.3 | 0.000108 | 0.02 | 0.645 |
INPP4B|INPP4B-G-E | 0.13 | 0.0001131 | 0.021 | 0.358 |
BAX|BAX-R-V | 5.1 | 0.0001661 | 0.03 | 0.669 |
IGFBP2|IGFBP2-R-V | 2.1 | 0.0001831 | 0.033 | 0.734 |
RAB11A RAB11B|RAB11-R-E | 0.04 | 0.0002407 | 0.043 | 0.31 |
AGE | Mean (SD) | 42.58 (13) |
Significant markers | N = 8 | |
pos. correlated | 6 | |
neg. correlated | 2 |
SpearmanCorr | corrP | Q | |
---|---|---|---|
IGFBP2|IGFBP2-R-V | 0.3146 | 3.062e-07 | 5.79e-05 |
PEA15|PEA15-R-V | -0.2931 | 1.999e-06 | 0.000376 |
SERPINE1|PAI-1-M-E | 0.2747 | 8.9e-06 | 0.00166 |
EGFR|EGFR-R-V | 0.214 | 0.000597 | 0.111 |
ASNS|ASNS-R-V | 0.2116 | 0.0006892 | 0.128 |
ANXA1|ANNEXIN-1-M-E | 0.209 | 0.0008039 | 0.148 |
CDKN1A|P21-R-V | 0.2087 | 0.0008182 | 0.15 |
CDH1|E-CADHERIN-R-V | -0.2 | 0.001352 | 0.246 |
GENDER | Labels | N |
FEMALE | 108 | |
MALE | 146 | |
Significant markers | N = 3 | |
Higher in MALE | 3 | |
Higher in FEMALE | 0 |
W(pos if higher in 'MALE') | wilcoxontestP | Q | AUC | |
---|---|---|---|---|
STAT5A|STAT5-ALPHA-R-V | 5649 | 0.0001133 | 0.0214 | 0.6417 |
RAB11A RAB11B|RAB11-R-E | 10021.5 | 0.0002227 | 0.0419 | 0.6356 |
TSC1|TSC1-R-C | 5974 | 0.0009711 | 0.182 | 0.6211 |
One gene related to 'KARNOFSKY.PERFORMANCE.SCORE'.
KARNOFSKY.PERFORMANCE.SCORE | Mean (SD) | 86.88 (12) |
Significant markers | N = 1 | |
pos. correlated | 0 | |
neg. correlated | 1 |
SpearmanCorr | corrP | Q | |
---|---|---|---|
IGFBP2|IGFBP2-R-V | -0.3378 | 0.00027 | 0.051 |
HISTOLOGICAL.TYPE | Labels | N |
ASTROCYTOMA | 83 | |
OLIGOASTROCYTOMA | 70 | |
OLIGODENDROGLIOMA | 101 | |
Significant markers | N = 46 |
ANOVA_P | Q | |
---|---|---|
SYK|SYK-M-V | 1.613e-15 | 3.05e-13 |
ANXA1|ANNEXIN-1-M-E | 1.157e-10 | 2.17e-08 |
AR|AR-R-V | 1.623e-08 | 3.04e-06 |
BAX|BAX-R-V | 7.397e-08 | 1.38e-05 |
MAPK14|P38_MAPK-R-V | 8.768e-08 | 1.62e-05 |
YAP1|YAP_PS127-R-E | 1.262e-07 | 2.32e-05 |
ERBB3|HER3_PY1289-R-C | 2.152e-07 | 3.94e-05 |
KIT|C-KIT-R-V | 1.073e-06 | 0.000195 |
PTEN|PTEN-R-V | 5.358e-06 | 0.00097 |
CDKN1B|P27_PT157-R-C | 6.585e-06 | 0.00119 |
No gene related to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
RADIATIONS.RADIATION.REGIMENINDICATION | Labels | N |
NO | 84 | |
YES | 170 | |
Significant markers | N = 0 |
RACE | Labels | N |
BLACK OR AFRICAN AMERICAN | 11 | |
WHITE | 242 | |
Significant markers | N = 0 |
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Expresson data file = LGG-TP.rppa.txt
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Clinical data file = LGG-TP.merged_data.txt
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Number of patients = 254
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Number of genes = 189
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Number of clinical features = 8
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.