This pipeline computes the correlation between cancer subtypes identified by different molecular patterns and selected clinical features.
Testing the association between subtypes identified by 8 different clustering approaches and 3 clinical features across 61 patients, 2 significant findings detected with P value < 0.05 and Q value < 0.25.
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5 subtypes identified in current cancer cohort by 'Copy Number Ratio CNMF subtypes'. These subtypes correlate to 'GENDER'.
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3 subtypes identified in current cancer cohort by 'METHLYATION CNMF'. These subtypes do not correlate to any clinical features.
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CNMF clustering analysis on sequencing-based mRNA expression data identified 4 subtypes that do not correlate to any clinical features.
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Consensus hierarchical clustering analysis on sequencing-based mRNA expression data identified 4 subtypes that do not correlate to any clinical features.
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3 subtypes identified in current cancer cohort by 'MIRSEQ CNMF'. These subtypes do not correlate to any clinical features.
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4 subtypes identified in current cancer cohort by 'MIRSEQ CHIERARCHICAL'. These subtypes correlate to 'GENDER'.
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3 subtypes identified in current cancer cohort by 'MIRseq Mature CNMF subtypes'. These subtypes do not correlate to any clinical features.
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3 subtypes identified in current cancer cohort by 'MIRseq Mature cHierClus subtypes'. These subtypes do not correlate to any clinical features.
Clinical Features |
AGE | GENDER | RACE |
Statistical Tests | Kruskal-Wallis (anova) | Fisher's exact test | Fisher's exact test |
Copy Number Ratio CNMF subtypes |
0.603 (1.00) |
0.00732 (0.176) |
0.89 (1.00) |
METHLYATION CNMF |
0.673 (1.00) |
0.129 (1.00) |
0.388 (1.00) |
RNAseq CNMF subtypes |
0.546 (1.00) |
0.0382 (0.802) |
0.214 (1.00) |
RNAseq cHierClus subtypes |
0.544 (1.00) |
0.0619 (1.00) |
0.445 (1.00) |
MIRSEQ CNMF |
0.616 (1.00) |
0.145 (1.00) |
0.489 (1.00) |
MIRSEQ CHIERARCHICAL |
0.513 (1.00) |
0.0107 (0.245) |
0.543 (1.00) |
MIRseq Mature CNMF subtypes |
0.239 (1.00) |
0.204 (1.00) |
0.224 (1.00) |
MIRseq Mature cHierClus subtypes |
0.338 (1.00) |
0.0186 (0.409) |
0.409 (1.00) |
Cluster Labels | 1 | 2 | 3 | 4 | 5 |
---|---|---|---|---|---|
Number of samples | 4 | 7 | 21 | 18 | 7 |
P value = 0.603 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 57 | 49.2 (13.8) |
subtype1 | 4 | 43.8 (18.1) |
subtype2 | 7 | 52.9 (12.5) |
subtype3 | 21 | 52.0 (14.5) |
subtype4 | 18 | 47.4 (14.4) |
subtype5 | 7 | 45.0 (9.4) |
P value = 0.00732 (Fisher's exact test), Q value = 0.18
nPatients | FEMALE | MALE |
---|---|---|
ALL | 37 | 20 |
subtype1 | 3 | 1 |
subtype2 | 2 | 5 |
subtype3 | 19 | 2 |
subtype4 | 9 | 9 |
subtype5 | 4 | 3 |
P value = 0.89 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 2 | 7 | 45 |
subtype1 | 0 | 0 | 0 | 3 |
subtype2 | 0 | 0 | 1 | 6 |
subtype3 | 1 | 1 | 3 | 15 |
subtype4 | 0 | 0 | 3 | 15 |
subtype5 | 0 | 1 | 0 | 6 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 14 | 28 | 19 |
P value = 0.673 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 14 | 46.2 (13.7) |
subtype2 | 28 | 51.2 (14.0) |
subtype3 | 19 | 48.8 (13.8) |
P value = 0.129 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 7 | 7 |
subtype2 | 22 | 6 |
subtype3 | 11 | 8 |
P value = 0.388 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 1 | 1 | 11 |
subtype2 | 1 | 0 | 5 | 21 |
subtype3 | 0 | 2 | 1 | 16 |
Cluster Labels | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Number of samples | 13 | 21 | 18 | 9 |
P value = 0.546 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 13 | 48.3 (14.2) |
subtype2 | 21 | 50.7 (12.8) |
subtype3 | 18 | 46.0 (13.4) |
subtype4 | 9 | 54.2 (16.5) |
P value = 0.0382 (Fisher's exact test), Q value = 0.8
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 10 | 3 |
subtype2 | 15 | 6 |
subtype3 | 7 | 11 |
subtype4 | 8 | 1 |
P value = 0.214 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 1 | 2 | 8 |
subtype2 | 1 | 0 | 3 | 17 |
subtype3 | 0 | 1 | 0 | 17 |
subtype4 | 0 | 1 | 2 | 6 |
Cluster Labels | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Number of samples | 14 | 25 | 13 | 9 |
P value = 0.544 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 14 | 50.1 (11.7) |
subtype2 | 25 | 50.4 (13.5) |
subtype3 | 13 | 43.8 (14.6) |
subtype4 | 9 | 53.1 (16.2) |
P value = 0.0619 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 8 | 6 |
subtype2 | 20 | 5 |
subtype3 | 5 | 8 |
subtype4 | 7 | 2 |
P value = 0.445 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 1 | 1 | 12 |
subtype2 | 1 | 0 | 5 | 18 |
subtype3 | 0 | 1 | 0 | 11 |
subtype4 | 0 | 1 | 1 | 7 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 25 | 20 | 16 |
P value = 0.616 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 25 | 46.9 (13.7) |
subtype2 | 20 | 50.1 (13.6) |
subtype3 | 16 | 52.1 (14.3) |
P value = 0.145 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 13 | 12 |
subtype2 | 16 | 4 |
subtype3 | 11 | 5 |
P value = 0.489 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 1 | 2 | 1 | 20 |
subtype2 | 0 | 0 | 3 | 16 |
subtype3 | 0 | 1 | 3 | 12 |
Cluster Labels | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Number of samples | 23 | 22 | 6 | 10 |
P value = 0.513 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 23 | 46.3 (12.7) |
subtype2 | 22 | 50.3 (14.4) |
subtype3 | 6 | 48.3 (12.2) |
subtype4 | 10 | 54.6 (15.6) |
P value = 0.0107 (Fisher's exact test), Q value = 0.25
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 10 | 13 |
subtype2 | 19 | 3 |
subtype3 | 3 | 3 |
subtype4 | 8 | 2 |
P value = 0.543 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 2 | 1 | 19 |
subtype2 | 1 | 0 | 3 | 17 |
subtype3 | 0 | 0 | 1 | 5 |
subtype4 | 0 | 1 | 2 | 7 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 14 | 24 | 23 |
P value = 0.239 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 14 | 43.3 (15.8) |
subtype2 | 24 | 52.0 (13.3) |
subtype3 | 23 | 50.2 (12.3) |
P value = 0.204 (Fisher's exact test), Q value = 1
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 8 | 6 |
subtype2 | 19 | 5 |
subtype3 | 13 | 10 |
P value = 0.224 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 1 | 0 | 12 |
subtype2 | 0 | 0 | 5 | 18 |
subtype3 | 1 | 2 | 2 | 18 |
Cluster Labels | 1 | 2 | 3 |
---|---|---|---|
Number of samples | 24 | 21 | 16 |
P value = 0.338 (Kruskal-Wallis (anova)), Q value = 1
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 61 | 49.3 (13.8) |
subtype1 | 24 | 45.6 (12.8) |
subtype2 | 21 | 51.3 (14.1) |
subtype3 | 16 | 52.2 (14.3) |
P value = 0.0186 (Fisher's exact test), Q value = 0.41
nPatients | FEMALE | MALE |
---|---|---|
ALL | 40 | 21 |
subtype1 | 11 | 13 |
subtype2 | 18 | 3 |
subtype3 | 11 | 5 |
P value = 0.409 (Fisher's exact test), Q value = 1
nPatients | AMERICAN INDIAN OR ALASKA NATIVE | ASIAN | BLACK OR AFRICAN AMERICAN | WHITE |
---|---|---|---|---|
ALL | 1 | 3 | 7 | 48 |
subtype1 | 0 | 2 | 1 | 20 |
subtype2 | 1 | 0 | 3 | 16 |
subtype3 | 0 | 1 | 3 | 12 |
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Cluster data file = PCPG-TP.mergedcluster.txt
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Clinical data file = PCPG-TP.merged_data.txt
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Number of patients = 61
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Number of clustering approaches = 8
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Number of selected clinical features = 3
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Exclude small clusters that include fewer than K patients, K = 3
consensus non-negative matrix factorization clustering approach (Brunet et al. 2004)
Resampling-based clustering method (Monti et al. 2003)
For binary clinical features, two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.