Clustering of miRseq precursor expression: consensus hierarchical
Acute Myeloid Leukemia (Primary blood derived cancer - Peripheral blood)
28 January 2016 | analyses__2016_01_28
Maintainer Information
Citation Information
doi:10.7908/C1V12468
Maintained by Hailei Zhang (Broad Institute)
Cite as Broad Institute TCGA Genome Data Analysis Center (2016): Clustering of miRseq precursor expression: consensus hierarchical. Broad Institute of MIT and Harvard. doi:10.7908/C1V12468
Overview
Introduction

This pipeline calculates clusters based on consensus hierarchical clustering with agglomerative ward linkage , . This pipeline has the following features:

1. Classify samples into consensus clusters.

2. Determine differentially expressed marker miRs for each subtype.

Summary

Median absolute deviation (MAD) was used to select 88 most variable miRs. Consensus ward linkage hierarchical clustering of 188 samples and 88 miRs identified 3 subtypes with the stability of the clustering increasing for k = 2 to k = 10.

Results
miR expression patterns of molecular subtypes

Figure 1. Get High-res Image Samples were separated into 3 clusters. Shown are 188 samples and 479 marker miRs. The color bar of the row indicates the marker miRs for the corresponding cluster.

Figure 2. Get High-res Image Heatmap with a standard hierarchical clustering for 188 samples and the 88 most variable miRs.

Silhouette widths, Cophenetic Correlation Coefficients and Consensus matrix

Figure 3. Get High-res Image The silhouette width was calculated for each sample and each value of k. The left upper panel shows the average silhouette width across all samples for each tested k (left upper panel). The left lower panel shows the Cophenetic Correlation Coefficients for each tested k. The right panel shows assignments of clusters to samples and the k silhouette width of each sample for the most robust clustering.

Figure 4. Get High-res Image The consensus matrix after clustering shows 3 clusters with limited overlap between clusters.

Samples assignment with silhouette width

Table 1. Get Full Table List of samples with 3 subtypes and silhouette width.

SampleName | cluster | silhouetteValue
--- | --- | ---
TCGA-AB-2802-03 | 1 | 0.4
TCGA-AB-2810-03 | 1 | 0.56
TCGA-AB-2811-03 | 1 | 0.14
TCGA-AB-2812-03 | 1 | 0.23
TCGA-AB-2816-03 | 1 | 0.16
TCGA-AB-2818-03 | 1 | 0.22
TCGA-AB-2824-03 | 1 | 0.37
TCGA-AB-2825-03 | 1 | 0.39
TCGA-AB-2826-03 | 1 | 0.3
TCGA-AB-2832-03 | 1 | 0.082

Table 2. Get Full Table List of samples belonging to each cluster in different k clusters.

SampleName | clu.2 | clu.3 | clu.4 | clu.5 | clu.6 | clu.7 | clu.8 | clu.9 | clu.10
--- | --- | --- | --- | --- | --- | --- | --- | --- | ---
TCGA-AB-2802-03 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1
TCGA-AB-2810-03 | 1 | 1 | 4 | 5 | 5 | 6 | 6 | 6 | 6
TCGA-AB-2811-03 | 1 | 1 | 4 | 5 | 5 | 6 | 7 | 7 | 7
TCGA-AB-2812-03 | 1 | 1 | 4 | 5 | 5 | 6 | 7 | 7 | 7
TCGA-AB-2816-03 | 1 | 1 | 4 | 5 | 5 | 6 | 7 | 7 | 7
TCGA-AB-2818-03 | 1 | 1 | 1 | 5 | 1 | 1 | 1 | 7 | 1
TCGA-AB-2824-03 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1
TCGA-AB-2825-03 | 1 | 1 | 1 | 5 | 5 | 6 | 7 | 7 | 7
TCGA-AB-2826-03 | 1 | 1 | 4 | 5 | 5 | 6 | 6 | 6 | 6
TCGA-AB-2832-03 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 9 | 10

Marker miRs of each subtype

Samples most representative of the clusters, hereby called core samples were identified based on positive silhouette width, indicating higher similarity to their own class than to any other class member. Core samples were used to select differentially expressed marker miRs for each subtype by comparing the subclass versus the other subclasses, using Student's t-test.

Table 3. Get Full Table List of marker miRs with p <= 0.05 (The positive value of column difference means miR is upregulated in this subtype and vice versa).

Composite.Element.REF | p | difference | q | subclass
--- | --- | --- | --- | ---
HSA-MIR-10A | 2e-17 | 4 | 1.4e-15 | 1
HSA-MIR-9-1 | 1.7e-16 | 3.6 | 9.3e-15 | 1
HSA-MIR-9-2 | 3.9e-16 | 3.5 | 1.9e-14 | 1
HSA-MIR-196B | 2.1e-17 | 3 | 1.4e-15 | 1
HSA-MIR-133A-1 | 0.000039 | 1.6 | 0.00013 | 1
HSA-MIR-1-2 | 0.0003 | 1.5 | 0.00084 | 1
HSA-LET-7B | 1.8e-17 | 1.4 | 1.4e-15 | 1
HSA-MIR-34A | 6.1e-06 | 1.1 | 0.000023 | 1
HSA-MIR-362 | 5.2e-06 | 0.9 | 0.000021 | 1
HSA-MIR-339 | 2e-08 | 0.86 | 1.9e-07 | 1

Methods & Data
Input

miRseq (at precursor level) of RPM value (reads per million reads aligned to miRBase precursor) with log2 transformed was as the input data for the clustering.

- Input file for selecting top 88 genes = *.miRseq_RPKM_log2.txt from miRseq_Preprocess

- Input file for the clustering module = /xchip/cga/gdac-prod/tcga-gdac/jobResults/GDAC_TopgenesforCluster/LAML-TB/22506934/LAML-TB.expclu.gct

Consensus Hierarchical Clustering

Consensus Hierarchical clustering is a resampling-based clustering. It provides for a method to represent the consensus across multiple runs of a clustering algorithm and to assess the stability of the discovered clusters. To this end, perturbations of the original data are simulated by resampling techniques. In our analysis, the R version of ConsensusClusterPlus(v1.18.0) was used , .

Silhouette Width

Silhouette width is defined as the ratio of average distance of each sample to samples in the same cluster to the smallest distance to samples not in the same cluster. If silhouette width is close to 1, it means that sample is well clustered. If silhouette width is close to -1, it means that sample is misclassified .

Cophenetic Correlation Coefficient and How to select the best cluster

The cophenetic correlation coefficient is computed as the Pearson correlation of two distance matrices:

1. Distance between samples induced by the consensus matrix.

2. Distance between samples induced by the linkage used in reordering the consensus matrix.

The cophenetic correlation coefficients and average silhouette values are used to determine the k with the most robust clusterings. From the plot of cophenetic correlation versus k, we select modes and the the point preceding the greatest decrease in cophenetic correlation coefficient, and from these choose the k with the highest average silhouette value.

Download Results

In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.

- Analysis Results (MD5 checksum)

- Auxiliary Data (MD5 checksum)

- MAGE-TAB File (MD5 checksum)

References
[1] Monti, S., Tamayo, P., Mesirov, J. & Golub, T.R., Consensus Clustering: A Resampling-Based Method for Class Discovery and Visualization of Gene Expression Microarray Data, Machine Learning:91-118 (2003)
[2] Wilkerson M and Waltman P, ConsensusClusterPlus: ConsensusClusterPlus
[3] Rousseeuw, P.J., Silhouettes: A graphical aid to the interpretation and validation of cluster analysis., J. Comput. Appl. Math. 20:53-65 (1987)
[4] Brunet, J.P., Tamayo, P., Golub, T.R. & Mesirov, J.P., Metagenes and molecular pattern discovery using matrix factorization, Proc Natl Acad Sci U S A 12(101):4164-9 (2004)
[5] R silhouette package
[6] RSEM
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