Correlation between copy number variation genes and selected clinical features

Lung Adenocarcinoma (MOLECULAR_NONSMOKER)

07 February 2013 | awg_luad__2013_02_07

Maintainer Information

Citation Information

Maintained by TCGA GDAC Team (Broad Institute/MD Anderson Cancer Center/Harvard Medical School)

Cite as Broad Institute TCGA Genome Data Analysis Center (2013): Correlation between copy number variation genes and selected clinical features. Broad Institute of MIT and Harvard. doi:10.7908/C1G44NC3

Overview

Introduction

This pipeline computes the correlation between significant copy number variation (cnv) genes and selected clinical features.

Summary

Testing the association between copy number variation of 42 peak regions and 14 clinical features across 80 patients, no significant finding detected with Q value < 0.25.

No gene cnvs related to clinical features.

Results

Overview of the results

Table 1. Get Full Table Overview of the association between significant copy number variation of 42 regions and 14 clinical features. Shown in the table are P values (Q values). Thresholded by Q value < 0.25, no significant finding detected.


		Clinical Features	Time to Death	AGE	GENDER	KARNOFSKY PERFORMANCE SCORE	HISTOLOGICAL TYPE	PATHOLOGY T	PATHOLOGY N	PATHOLOGICSPREAD(M)	TUMOR STAGE	RADIATIONS RADIATION REGIMENINDICATION	NUMBERPACKYEARSSMOKED	STOPPEDSMOKINGYEAR	TOBACCOSMOKINGHISTORYINDICATOR	YEAROFTOBACCOSMOKINGONSET
	nCNV (%)	nWild-Type	logrank test	t-test	Fisher's exact test	t-test	Chi-square test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	Fisher's exact test	t-test	t-test	Fisher's exact test	t-test
Amp Peak 1(1q21 3)	48 (60%)	32	0.554 (1.00)	0.08 (1.00)	0.0401 (1.00)		0.2 (1.00)	0.0892 (1.00)	0.946 (1.00)	0.758 (1.00)	0.91 (1.00)	0.643 (1.00)	0.261 (1.00)	0.614 (1.00)	0.557 (1.00)	0.367 (1.00)
Amp Peak 2(3q26 31)	23 (29%)	57	0.91 (1.00)	0.575 (1.00)	0.805 (1.00)		0.672 (1.00)	0.935 (1.00)	0.824 (1.00)	0.478 (1.00)	1 (1.00)	1 (1.00)	0.577 (1.00)	0.949 (1.00)	0.326 (1.00)	0.639 (1.00)
Amp Peak 3(5p15 33)	48 (60%)	32	0.277 (1.00)	0.0287 (1.00)	0.356 (1.00)		0.442 (1.00)	0.456 (1.00)	0.367 (1.00)	0.686 (1.00)	0.385 (1.00)	0.643 (1.00)	0.0607 (1.00)	0.426 (1.00)	0.698 (1.00)	0.547 (1.00)
Amp Peak 4(7p21 1)	50 (62%)	30	0.458 (1.00)	0.837 (1.00)	0.353 (1.00)		0.185 (1.00)	0.501 (1.00)	0.225 (1.00)	0.323 (1.00)	0.797 (1.00)	1 (1.00)	0.874 (1.00)	0.649 (1.00)	0.587 (1.00)	0.732 (1.00)
Amp Peak 5(7p11 2)	49 (61%)	31	0.254 (1.00)	0.755 (1.00)	0.817 (1.00)		0.101 (1.00)	0.71 (1.00)	0.0435 (1.00)	0.217 (1.00)	0.554 (1.00)	1 (1.00)	0.893 (1.00)	0.862 (1.00)	0.868 (1.00)	0.492 (1.00)
Amp Peak 6(7q31 2)	34 (42%)	46	0.816 (1.00)	0.421 (1.00)	0.648 (1.00)		0.162 (1.00)	0.536 (1.00)	0.261 (1.00)	0.00685 (1.00)	0.309 (1.00)	0.157 (1.00)	0.818 (1.00)	0.591 (1.00)	0.641 (1.00)	0.715 (1.00)
Amp Peak 7(8p11 21)	23 (29%)	57	0.522 (1.00)	0.337 (1.00)	0.805 (1.00)		0.557 (1.00)	0.552 (1.00)	0.938 (1.00)	0.893 (1.00)	0.869 (1.00)	0.622 (1.00)	0.528 (1.00)	0.0273 (1.00)	0.613 (1.00)	0.303 (1.00)
Amp Peak 8(8q24 21)	46 (58%)	34	0.808 (1.00)	0.573 (1.00)	0.116 (1.00)		0.355 (1.00)	0.848 (1.00)	0.68 (1.00)	0.357 (1.00)	0.59 (1.00)	1 (1.00)	0.0467 (1.00)	0.205 (1.00)	0.77 (1.00)	0.684 (1.00)
Amp Peak 9(11q22 1)	26 (32%)	54	0.811 (1.00)	0.0414 (1.00)	0.638 (1.00)		0.839 (1.00)	0.467 (1.00)	0.125 (1.00)	0.726 (1.00)	0.172 (1.00)	1 (1.00)	0.154 (1.00)	0.0371 (1.00)	0.0272 (1.00)	0.997 (1.00)
Amp Peak 10(12p12 1)	23 (29%)	57	0.0576 (1.00)	0.25 (1.00)	0.321 (1.00)		0.886 (1.00)	0.266 (1.00)	0.301 (1.00)	0.00793 (1.00)	0.477 (1.00)	1 (1.00)	0.608 (1.00)	0.862 (1.00)	0.882 (1.00)	0.849 (1.00)
Amp Peak 11(12q15)	25 (31%)	55	0.641 (1.00)	0.239 (1.00)	0.627 (1.00)		0.769 (1.00)	0.83 (1.00)	0.176 (1.00)	0.086 (1.00)	0.71 (1.00)	0.318 (1.00)	0.0403 (1.00)	0.992 (1.00)	0.535 (1.00)	0.463 (1.00)
Amp Peak 12(14q13 3)	33 (41%)	47	0.115 (1.00)	0.693 (1.00)	0.178 (1.00)		0.537 (1.00)	0.481 (1.00)	0.896 (1.00)	0.917 (1.00)	0.777 (1.00)	0.644 (1.00)	0.87 (1.00)	0.514 (1.00)	0.834 (1.00)	0.77 (1.00)
Amp Peak 13(17q12)	41 (51%)	39	0.439 (1.00)	0.972 (1.00)	1 (1.00)		0.47 (1.00)	0.946 (1.00)	0.651 (1.00)	0.04 (1.00)	0.535 (1.00)	0.36 (1.00)	0.403 (1.00)	0.322 (1.00)	0.669 (1.00)	0.668 (1.00)
Amp Peak 14(19q13 11)	17 (21%)	63	0.366 (1.00)	0.843 (1.00)	0.0123 (1.00)		0.149 (1.00)	1 (1.00)	0.575 (1.00)	0.589 (1.00)	0.694 (1.00)	0.579 (1.00)	0.554 (1.00)	0.176 (1.00)	0.61 (1.00)	0.828 (1.00)
Amp Peak 15(20p11 22)	24 (30%)	56	0.286 (1.00)	0.177 (1.00)	0.0135 (1.00)		0.618 (1.00)	0.0237 (1.00)	0.588 (1.00)	0.363 (1.00)	0.716 (1.00)	1 (1.00)	0.662 (1.00)	0.651 (1.00)	0.918 (1.00)	0.302 (1.00)
Amp Peak 16(20q13 33)	38 (48%)	42	0.944 (1.00)	0.271 (1.00)	0.179 (1.00)		0.508 (1.00)	0.0524 (1.00)	0.469 (1.00)	0.0251 (1.00)	0.00634 (1.00)	0.362 (1.00)	0.17 (1.00)	0.461 (1.00)	0.386 (1.00)	0.559 (1.00)
Del Peak 1(1p36 12)	20 (25%)	60	0.0856 (1.00)	0.452 (1.00)	0.035 (1.00)		0.519 (1.00)	0.456 (1.00)	0.0401 (1.00)	0.287 (1.00)	0.147 (1.00)	1 (1.00)	0.241 (1.00)	0.989 (1.00)	0.17 (1.00)	0.49 (1.00)
Del Peak 2(1p12)	23 (29%)	57	0.841 (1.00)	0.0589 (1.00)	0.62 (1.00)		0.101 (1.00)	0.482 (1.00)	0.825 (1.00)	0.373 (1.00)	0.169 (1.00)	1 (1.00)	0.219 (1.00)	0.761 (1.00)	0.381 (1.00)	0.00713 (1.00)
Del Peak 3(3p22 1)	31 (39%)	49	0.0508 (1.00)	0.392 (1.00)	1 (1.00)		0.0616 (1.00)	0.473 (1.00)	0.537 (1.00)	0.561 (1.00)	0.878 (1.00)	0.644 (1.00)	0.0763 (1.00)	0.761 (1.00)	0.773 (1.00)	0.0848 (1.00)
Del Peak 4(5q11 2)	22 (28%)	58	0.285 (1.00)	0.0948 (1.00)	0.803 (1.00)		0.527 (1.00)	0.0537 (1.00)	0.507 (1.00)	0.646 (1.00)	0.595 (1.00)	1 (1.00)	0.408 (1.00)	0.22 (1.00)	0.657 (1.00)	0.872 (1.00)
Del Peak 5(6q11 1)	18 (22%)	62	0.0663 (1.00)	0.786 (1.00)	1 (1.00)		0.423 (1.00)	0.682 (1.00)	0.744 (1.00)	0.2 (1.00)	0.558 (1.00)	0.313 (1.00)	0.722 (1.00)	0.234 (1.00)	0.379 (1.00)	0.368 (1.00)
Del Peak 6(6q26)	34 (42%)	46	0.684 (1.00)	0.369 (1.00)	0.262 (1.00)		0.218 (1.00)	0.756 (1.00)	0.611 (1.00)	0.562 (1.00)	0.415 (1.00)	0.646 (1.00)	0.302 (1.00)	0.0561 (1.00)	0.439 (1.00)	0.934 (1.00)
Del Peak 7(8p22)	36 (45%)	44	0.0261 (1.00)	0.337 (1.00)	0.174 (1.00)		0.251 (1.00)	0.108 (1.00)	0.721 (1.00)	0.764 (1.00)	0.962 (1.00)	0.372 (1.00)	0.0355 (1.00)	0.0899 (1.00)	0.707 (1.00)	0.755 (1.00)
Del Peak 8(9p23)	50 (62%)	30	0.98 (1.00)	0.0979 (1.00)	1 (1.00)		0.317 (1.00)	0.288 (1.00)	0.334 (1.00)	1 (1.00)	0.248 (1.00)	1 (1.00)	0.523 (1.00)	0.115 (1.00)	0.0272 (1.00)	0.364 (1.00)
Del Peak 9(9p21 3)	53 (66%)	27	0.337 (1.00)	0.0925 (1.00)	0.339 (1.00)		0.576 (1.00)	0.306 (1.00)	0.835 (1.00)	0.598 (1.00)	0.482 (1.00)	1 (1.00)	0.536 (1.00)	0.0875 (1.00)	0.0515 (1.00)	0.364 (1.00)
Del Peak 10(9q21 11)	40 (50%)	40	0.467 (1.00)	0.402 (1.00)	0.498 (1.00)		0.109 (1.00)	0.0595 (1.00)	0.155 (1.00)	0.646 (1.00)	0.13 (1.00)	1 (1.00)	0.539 (1.00)	0.941 (1.00)	0.498 (1.00)	0.178 (1.00)
Del Peak 11(10p15 3)	25 (31%)	55	0.4 (1.00)	0.352 (1.00)	0.00141 (0.767)		0.391 (1.00)	0.606 (1.00)	0.882 (1.00)	0.902 (1.00)	0.896 (1.00)	1 (1.00)	0.15 (1.00)	0.955 (1.00)	0.0263 (1.00)	0.342 (1.00)
Del Peak 12(10q26 3)	25 (31%)	55	0.654 (1.00)	0.933 (1.00)	0.00738 (1.00)		0.846 (1.00)	0.176 (1.00)	0.687 (1.00)	1 (1.00)	0.457 (1.00)	0.318 (1.00)	0.671 (1.00)	0.986 (1.00)	0.102 (1.00)	0.566 (1.00)
Del Peak 13(12p13 2)	23 (29%)	57	0.799 (1.00)	0.855 (1.00)	0.0809 (1.00)		0.183 (1.00)	0.326 (1.00)	0.0455 (1.00)	0.893 (1.00)	0.00512 (1.00)	0.14 (1.00)	0.0573 (1.00)	0.607 (1.00)	0.405 (1.00)	0.143 (1.00)
Del Peak 14(12q23 1)	22 (28%)	58	0.499 (1.00)	0.617 (1.00)	0.615 (1.00)		0.0768 (1.00)	0.333 (1.00)	0.879 (1.00)	0.205 (1.00)	0.451 (1.00)	1 (1.00)	0.000539 (0.294)	0.411 (1.00)	0.63 (1.00)	0.888 (1.00)
Del Peak 15(13q12 11)	41 (51%)	39	0.399 (1.00)	0.864 (1.00)	1 (1.00)		0.328 (1.00)	0.613 (1.00)	0.807 (1.00)	0.435 (1.00)	0.626 (1.00)	0.195 (1.00)	0.823 (1.00)	0.98 (1.00)	0.576 (1.00)	0.255 (1.00)
Del Peak 16(15q14)	31 (39%)	49	0.248 (1.00)	0.311 (1.00)	0.0654 (1.00)		0.365 (1.00)	0.117 (1.00)	0.895 (1.00)	1 (1.00)	0.711 (1.00)	1 (1.00)	0.202 (1.00)	0.681 (1.00)	0.369 (1.00)	0.966 (1.00)
Del Peak 17(16q12 1)	17 (21%)	63	0.85 (1.00)	0.283 (1.00)	0.41 (1.00)		0.201 (1.00)	0.436 (1.00)	0.921 (1.00)	0.244 (1.00)	1 (1.00)	0.579 (1.00)	0.0409 (1.00)	0.492 (1.00)	0.895 (1.00)	0.801 (1.00)
Del Peak 18(17p13 3)	48 (60%)	32	0.249 (1.00)	0.714 (1.00)	0.356 (1.00)		0.477 (1.00)	0.264 (1.00)	0.36 (1.00)	0.451 (1.00)	0.561 (1.00)	0.151 (1.00)	0.341 (1.00)	0.256 (1.00)	0.0446 (1.00)	0.586 (1.00)
Del Peak 19(17p13 2)	48 (60%)	32	0.249 (1.00)	0.714 (1.00)	0.356 (1.00)		0.477 (1.00)	0.264 (1.00)	0.36 (1.00)	0.451 (1.00)	0.561 (1.00)	0.151 (1.00)	0.341 (1.00)	0.256 (1.00)	0.0446 (1.00)	0.586 (1.00)
Del Peak 20(17p11 2)	35 (44%)	45	0.581 (1.00)	0.854 (1.00)	0.653 (1.00)		0.157 (1.00)	0.434 (1.00)	0.804 (1.00)	0.696 (1.00)	0.676 (1.00)	0.0643 (1.00)	0.953 (1.00)	0.772 (1.00)	0.921 (1.00)	0.574 (1.00)
Del Peak 21(18q21 1)	39 (49%)	41	0.887 (1.00)	0.632 (1.00)	0.651 (1.00)		0.568 (1.00)	0.0386 (1.00)	0.651 (1.00)	0.644 (1.00)	0.474 (1.00)	0.36 (1.00)	0.709 (1.00)	0.114 (1.00)	0.0369 (1.00)	0.838 (1.00)
Del Peak 22(19p13 3)	36 (45%)	44	0.546 (1.00)	0.417 (1.00)	0.26 (1.00)		0.045 (1.00)	1 (1.00)	0.686 (1.00)	0.914 (1.00)	1 (1.00)	1 (1.00)	0.406 (1.00)	0.276 (1.00)	0.407 (1.00)	0.543 (1.00)
Del Peak 23(19q13 32)	25 (31%)	55	0.155 (1.00)	0.469 (1.00)	0.811 (1.00)		0.00965 (1.00)	0.322 (1.00)	0.608 (1.00)	0.902 (1.00)	0.896 (1.00)	0.645 (1.00)	0.994 (1.00)	0.992 (1.00)	0.79 (1.00)	0.498 (1.00)
Del Peak 24(21q11 2)	21 (26%)	59	0.0774 (1.00)	0.416 (1.00)	1 (1.00)		0.167 (1.00)	0.874 (1.00)	0.116 (1.00)	0.787 (1.00)	0.759 (1.00)	1 (1.00)	0.326 (1.00)	0.819 (1.00)	0.615 (1.00)	0.844 (1.00)
Del Peak 25(22q13 31)	25 (31%)	55	0.281 (1.00)	0.0209 (1.00)	0.143 (1.00)		0.855 (1.00)	0.245 (1.00)	0.0504 (1.00)	0.433 (1.00)	0.00849 (1.00)	1 (1.00)	0.474 (1.00)	0.142 (1.00)	0.372 (1.00)	0.378 (1.00)
Del Peak 26(Xp21 3)	17 (21%)	63	0.326 (1.00)	1 (1.00)	0.784 (1.00)		0.345 (1.00)	0.628 (1.00)	0.388 (1.00)	0.882 (1.00)	0.344 (1.00)	1 (1.00)	0.667 (1.00)	0.676 (1.00)	0.529 (1.00)	0.887 (1.00)

Methods & Data

Input

Copy number data file = All Lesions File (all_lesions.conf_##.txt, where ## is the confidence level). The all lesions file is from GISTIC pipeline and summarizes the results from the GISTIC run. It contains data about the significant regions of amplification and deletion as well as which samples are amplified or deleted in each of these regions. The identified regions are listed down the first column, and the samples are listed across the first row, starting in column 10.
Clinical data file = MOLECULAR_NONSMOKER.clin.merged.picked.txt
Number of patients = 80
Number of copy number variation regions = 42
Number of selected clinical features = 14
Exclude regions that fewer than K tumors have alterations, K = 3

Survival analysis

For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene cnvs were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R

Student's t-test analysis

For continuous numerical clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the clinical values between tumors with and without gene cnvs using 't.test' function in R

Fisher's exact test

For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R

Chi-square test

For multi-class clinical features (nominal or ordinal), Chi-square tests (Greenwood and Nikulin 1996) were used to estimate the P values using the 'chisq.test' function in R

Q value calculation

For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.

Download Results

This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.

References

[1] Bland and Altman, Statistics notes: The logrank test, BMJ 328(7447):1073 (2004)

[2] Lehmann and Romano, Testing Statistical Hypotheses (3E ed.), New York: Springer. ISBN 0387988645 (2005)

[3] Fisher, R.A., On the interpretation of chi-square from contingency tables, and the calculation of P, Journal of the Royal Statistical Society 85(1):87-94 (1922)

[4] Greenwood and Nikulin, A guide to chi-squared testing, Wiley, New York. ISBN 047155779X (1996)

[5] Benjamini and Hochberg, Controlling the false discovery rate: a practical and powerful approach to multiple testing, Journal of the Royal Statistical Society Series B 59:289-300 (1995)

[6] GISTIC_version2

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