(follicular cohort)
This pipeline computes the correlation between significant copy number variation (cnv) genes and molecular subtypes.
Testing the association between copy number variation of 4 peak regions and 6 molecular subtypes across 75 patients, 7 significant findings detected with Q value < 0.25.
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Del Peak 1(3q13.31) cnvs correlated to 'CN_CNMF'.
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Del Peak 5(10q23.31) cnvs correlated to 'CN_CNMF' and 'MRNASEQ_CNMF'.
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Del Peak 7(22q11.21) cnvs correlated to 'CN_CNMF', 'METHLYATION_CNMF', and 'MRNASEQ_CHIERARCHICAL'.
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Del Peak 8(Xq22.3) cnvs correlated to 'CN_CNMF'.
Molecular subtypes |
CN CNMF |
METHLYATION CNMF |
MRNASEQ CNMF |
MRNASEQ CHIERARCHICAL |
MIRSEQ CNMF |
MIRSEQ CHIERARCHICAL |
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nCNV (%) | nWild-Type | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | |
Del Peak 7(22q11 21) | 17 (23%) | 58 |
5.76e-15 (1.38e-13) |
0.00531 (0.117) |
0.0608 (0.851) |
0.00669 (0.134) |
0.0984 (1.00) |
0.669 (1.00) |
Del Peak 5(10q23 31) | 6 (8%) | 69 |
5.42e-06 (0.000125) |
0.102 (1.00) |
0.00608 (0.128) |
0.351 (1.00) |
0.165 (1.00) |
0.0202 (0.324) |
Del Peak 1(3q13 31) | 3 (4%) | 72 |
0.00871 (0.165) |
0.0267 (0.4) |
0.113 (1.00) |
0.102 (1.00) |
0.346 (1.00) |
0.175 (1.00) |
Del Peak 8(Xq22 3) | 3 (4%) | 72 |
0.00871 (0.165) |
0.456 (1.00) |
0.0148 (0.251) |
0.768 (1.00) |
0.613 (1.00) |
0.596 (1.00) |
P value = 0.00871 (Fisher's exact test), Q value = 0.17
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 48 | 8 | 19 |
DEL PEAK 1(3Q13.31) MUTATED | 0 | 2 | 1 |
DEL PEAK 1(3Q13.31) WILD-TYPE | 48 | 6 | 18 |
P value = 5.42e-06 (Fisher's exact test), Q value = 0.00012
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 48 | 8 | 19 |
DEL PEAK 5(10Q23.31) MUTATED | 0 | 5 | 1 |
DEL PEAK 5(10Q23.31) WILD-TYPE | 48 | 3 | 18 |
P value = 0.00608 (Fisher's exact test), Q value = 0.13
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 17 | 25 | 18 | 11 |
DEL PEAK 5(10Q23.31) MUTATED | 4 | 0 | 0 | 2 |
DEL PEAK 5(10Q23.31) WILD-TYPE | 13 | 25 | 18 | 9 |
P value = 5.76e-15 (Fisher's exact test), Q value = 1.4e-13
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 48 | 8 | 19 |
DEL PEAK 7(22Q11.21) MUTATED | 0 | 0 | 17 |
DEL PEAK 7(22Q11.21) WILD-TYPE | 48 | 8 | 2 |
P value = 0.00531 (Fisher's exact test), Q value = 0.12
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 17 | 29 | 21 | 8 |
DEL PEAK 7(22Q11.21) MUTATED | 2 | 13 | 2 | 0 |
DEL PEAK 7(22Q11.21) WILD-TYPE | 15 | 16 | 19 | 8 |
P value = 0.00669 (Fisher's exact test), Q value = 0.13
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 23 | 24 | 24 |
DEL PEAK 7(22Q11.21) MUTATED | 5 | 10 | 1 |
DEL PEAK 7(22Q11.21) WILD-TYPE | 18 | 14 | 23 |
P value = 0.00871 (Fisher's exact test), Q value = 0.17
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 48 | 8 | 19 |
DEL PEAK 8(XQ22.3) MUTATED | 0 | 2 | 1 |
DEL PEAK 8(XQ22.3) WILD-TYPE | 48 | 6 | 18 |
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Copy number data file = All Lesions File (all_lesions.conf_##.txt, where ## is the confidence level). The all lesions file is from GISTIC pipeline and summarizes the results from the GISTIC run. It contains data about the significant regions of amplification and deletion as well as which samples are amplified or deleted in each of these regions. The identified regions are listed down the first column, and the samples are listed across the first row, starting in column 10.
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Molecular subtype file = THCA-follicular.transferedmergedcluster.txt
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Number of patients = 75
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Number of copy number variation regions = 4
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Number of molecular subtypes = 6
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Exclude regions that fewer than K tumors have alterations, K = 3
For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.