Uveal Melanoma (UVM) Samples Report
Overview
Introduction

The Broad GDAC mirrors data from the DCC on a daily basis. Although all data is mirrored, not every sample is ingested into Firehose. There are three main mechanisms that filter samples to ensure that only the most scientifically relevant samples make it into our standard data and analyses runs. These three mechanisms are redactions, replicate filtering, and blacklisting. This report summarizes the data that is ingested into Firehose, describes the three filtering mechanisms, lists those samples that are removed, and gives all available annotations from the DCC's Annotation Manager.

Summary

There were 0 redactions, 0 replicate aliquots, 0 blacklisted aliquots, and 0 FFPE aliquots. The table below represents the sample counts for those samples that were ingested into firehose after filtering out redactions, replicates, and blacklisted data, and segregating FFPEs.

Table 1.  This table provides a breakdown of sample counts on a per sample type and, if applicable, per subtype basis. Each count is a link to a table containing a list of the samples that comprise that count and details pertaining to each individual sample (e.g. platform, sequencing center, etc.). Please note, there are usually multiple protocols per data type, so there are typically many more rows than the count implies.

Sample Type BCR Clinical CN LowP Methylation mRNA mRNASeq miR miRSeq RPPA MAF
TP 80 0 0 0 0 0 0 0 0 0 0
Totals 80 0 0 0 0 0 0 0 0 0 0
UVM Primary Solid Tumor BCR Data

Table S1. 

TCGA Barcode Platform Center Data Level Protocol
TCGA-RZ-AB0B Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V3-A9ZX Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V3-A9ZY Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9E5 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9E7 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9E8 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9E9 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EA Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EC Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9ED Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EE Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EF Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EH Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EI Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EJ Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EK Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EL Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EM Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EO Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EQ Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9ES Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9ET Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EU Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EV Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EW Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EX Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EY Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9EZ Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F0 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F1 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F2 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F3 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F4 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F5 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F7 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-V4-A9F8 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8K7 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8K8 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8K9 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KA Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KB Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KD Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KE Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KF Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KG Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KH Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KI Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KJ Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KK Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KL Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KM Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KN Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-A8KO Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8M Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8N Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8O Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8P Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8Q Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8R Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8S Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-VD-AA8T Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A87T Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A87U Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A87W Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A87Y Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A880 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A881 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A882 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A883 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A884 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A885 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A888 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-A88A Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-AA9A Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-WC-AA9E Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-YZ-A980 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-YZ-A982 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-YZ-A983 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-YZ-A984 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen
TCGA-YZ-A985 Biospecimen Metadata - Complete Set Nationwide Children's Hospital 1 biospecimen

The sample type short letter codes in the table above are defined in the following list.

  • TP: Primary Solid Tumor

  • TR: Recurrent Solid Tumor

  • TB: Primary Blood Derived Cancer - Peripheral Blood

  • TAP: Additional - New Primary

  • TM: Metastatic

  • TAM: Additional Metastatic

  • NB: Blood Derived Normal

  • NT: Solid Tissue Normal

The following platforms are outdated and are not included in the counts depicted in the table above.

  • Agilent SurePrint G3 Human CGH Microarray Kit 1x1M

  • Agilent Human Genome CGH Microarray 244A

  • Agilent Human Genome CGH Custom Microarray 2x415K

  • Affymetrix Human Exon 1.0 ST Array

  • Illumina DNA Methylation OMA002 Cancer Panel I

  • Illumina DNA Methylation OMA003 Cancer Panel I

  • Illumina Human1M-Duo BeadChip

  • Illumina 550K Infinium HumanHap550 SNP Chip

Figure 1.  Get High-res Image This figure depicts the distribution of available data on a per participant basis.

Results
FFPE Cases
Additional Annotations from the DCC's Annotations Manager
Methods & Data
Redactions and Other Annotations

Annotation data was taken from theTCGA Data Portalusing the query string:

https://tcga-data.nci.nih.gov/annotations/resources/searchannotations/json?item=TCGA

Redaction information was generated by filtering for the annotationClassificationName "Redaction"

FFPE information was generated by filtering for "FFPE" in annotation note text

Additional FFPEs were garnered from clinical data

Remaining annotations were sorted into sections by annotationClassificationName

Preprocessors
mRNA Preprocessor

The mRNA preprocess median module chooses the matrix for the platform(Affymetrix HG U133, Affymetrix Exon Array and Agilent Gene Expression) with the largest number of samples.

mRNAseq Preprocessor

The mRNAseq preprocessor picks the "scaled_estimate" (RSEM) value from Illumina HiSeq/GA2 mRNAseq level_3 (v2) data set and makes the mRNAseq matrix with log2 transformed for the downstream analysis. If there are overlap samples between two different platforms, samples from illumina hiseq will be selected. The pipeline also creates the matrix with RPKM and log2 transform from HiSeq/GA2 mRNAseq level 3 (v1) data set.

miRseq Preprocessor

The miRseq preprocessor picks the "RPM" (reads per million miRNA precursor reads) from the Illumina HiSeq/GA miRseq Level_3 data set and makes the matrix with log2 transformed values.

Methylation Preprocessor

The methylation preprocessor filters methylation data for use in downstream pipelines. To learn more about this preprocessor, please visit the documentation.