This pipeline maps genes, with mutation or copy number alteration AND this alteration is highly correlated with mRNA expression, to pathways curated in the KEGG and BIOCARTA databases. It identifies pathways significantly enriched with these genes. The pipeline also identifies pathways significantly enriched with marker genes of each expression subtype of cancer.
genes with mutation: identified by the Mutation_Significance pipeline
genes with copy number alteration: identified by the CopyNumber_Gistic2 pipeline
correlation between copy number and mRNA expression: identified by the Correlate_CopyNumber_vs_mRNA pipeline
marker genes and expression subtypes: identified by the mRNAConsensusClustering pipeline
There are 353 genes with significant mutation (Q value <= 0.1) and 504 genes with significant copy number alteration (Q value <= 0.25). The identified marker genes (Q value <= 0.01 or within top 2000) are 1580 for subtype 1, 1580 for subtype 2, 1580 for subtype 3. Pathways significantly enriched with these genes (Q value <= 0.01) are identified :
3 pathways significantly enriched with genes with copy number alteration or mutation.
4 pathways significantly enriched with marker genes of gene expression subtype 1
2 pathways significantly enriched with marker genes of gene expression subtype 2
11 pathways significantly enriched with marker genes of gene expression subtype 3
Table 1. Get Full Table Top Pathways enriched with genes with copy number alteration or mutation. Nof Genes : No. of genes in this pathway. Nof CNV_Mut : No. of genes with copy number alteration or mutation in this pathway. Enrichment , P value and Q value : See Methods & Data below. CNV_Mut Genes in Pathway: genes with copy number alteration or mutation in this pathway.
| Pathway | Nof Genes | Nof CNV_Mut | Enrichment | P value | Q value |
|---|---|---|---|---|---|
| KEGG_OLFACTORY_TRANSDUCTION | 389 | 42 | 1.4 | 0 | 0 |
| KEGG_OXIDATIVE_PHOSPHORYLATION | 133 | 16 | 1.8 | 0 | 0.0025 |
| KEGG_PARKINSONS_DISEASE | 130 | 15 | 1.7 | 0 | 0.0044 |
| KEGG_RIBOSOME | 87 | 12 | 1.8 | 0.0002 | 0.015 |
| KEGG_PROTEASOME | 48 | 8 | 2.2 | 0.0004 | 0.023 |
OR4S2,OR4N4,OR4N2,OR5D16,PDE1C,OR5B12,OR4Q3,OR10R2,OR5T3,OR10Q1,OR4P4,OR51B2,OR5M11,OR52E2,OR52E6,OR52J3,OR8H3,OR8I2,OR8H1,OR4C6,OR4D2,OR4D5,OR5F1,OR2A5,OR9A2,OR5B3,OR5L1,OR5L2,OR4M1,OR5M9,OR10AG1,OR4K5,OR4K1,OR4A15,OR4A16,OR6K3,OR4C16,OR4C15,OR4C12,OR4C11,OR5H6,OR5J2
UQCRFS1,NDUFS6,ATP5I,NDUFS2,COX17,NDUFB10,COX4I1,ATP6V1F,ATP6V1A,NDUFB5,COX7B2,NDUFB2,NDUFA5,NDUFA7,ATP6V1E1,SDHC
SLC6A3,LRRK2,UQCRFS1,NDUFS6,NDUFS2,NDUFB10,COX4I1,NDUFB5,COX7B2,NDUFB2,NDUFA5,NDUFA7,PARK2,PARK7,SDHC
RPL18,RPL36AL,RPL35A,RPS19,RPS16,RPS15,MRPS12,RPS28,RPL24,RPS5,RPL28,MRPL23
PSMB5,PSMB4,PSMD2,PSMD7,PSMD8,PSMC6,PSMA6,PSMC4
Table 2. Get Full Table Top Pathways enriched with marker genes of gene expression subtype 1 . Nof Genes : No. of genes in this pathway. Nof Marker : No. of marker genes of gene expression subtype 1 in this pathway. Enrichment , P value and Q value : See Methods & Data below. Marker Gene in Pathway: markers of gene expression subtype 1 in this pathway
| Pathway | Nof Genes | Nof Marker | Enrichment | P value | Q value |
|---|---|---|---|---|---|
| KEGG_DRUG_METABOLISM_CYTOCHROME_P450 | 72 | 16 | 1.7 | 0 | 0.0038 |
| KEGG_AXON_GUIDANCE | 129 | 23 | 1.4 | 0 | 0.0038 |
| KEGG_GLYCOLYSIS_GLUCONEOGENESIS | 62 | 14 | 1.7 | 0.0001 | 0.008 |
| KEGG_GLUTATHIONE_METABOLISM | 50 | 12 | 1.8 | 0.0001 | 0.0085 |
| KEGG_METABOLISM_OF_XENOBIOTICS_BY_CYTOCHROME_P450 | 70 | 14 | 1.5 | 0.0002 | 0.014 |
CYP2C19,ADH1C,GSTM1,GSTM2,GSTM3,ALDH3B2,ALDH3B1,ADH5,FMO1,FMO2,FMO3,GSTO1,GSTA2,GSTA3,GSTA4,GSTA5
PLXNA1,ROBO2,PLXNB1,EFNB2,MAPK1,GNAI1,EPHB3,EPHB6,EPHA1,NTN1,RGS3,SEMA3F,SEMA3E,SEMA3B,PAK1,SLIT1,SEMA4G,NCK1,SEMA4A,ABLIM1,CHP,FYN,GSK3B
PGAM1,ADH1C,ACSS1,FBP1,ALDH3B2,ALDH3B1,ALDH7A1,DLD,ADH5,HK2,HK1,ENO3,BPGM,PCK1
GSTM1,GPX2,GSTM2,GSR,GSTM3,GCLC,GSTO1,GSTA2,GSTA3,GSTA4,GSTA5,GGT7
CYP2C19,ADH1C,GSTM1,GSTM2,GSTM3,AKR1C1,ALDH3B2,ALDH3B1,ADH5,GSTO1,GSTA2,GSTA3,GSTA4,GSTA5
Table 3. Get Full Table Top Pathways enriched with marker genes of gene expression subtype 2 . Nof Genes : No. of genes in this pathway. Nof Marker : No. of marker genes of gene expression subtype 2 in this pathway. Enrichment , P value and Q value : See Methods & Data below. Marker Gene in Pathway: markers of gene expression subtype 2 in this pathway
| Pathway | Nof Genes | Nof Marker | Enrichment | P value | Q value |
|---|---|---|---|---|---|
| KEGG_ENDOCYTOSIS | 183 | 39 | 1.3 | 0 | 0 |
| KEGG_ARRHYTHMOGENIC_RIGHT_VENTRICULAR_CARDIOMYOPATHY_ARVC | 76 | 18 | 1.5 | 0 | 0.0058 |
| BIOCARTA_AGR_PATHWAY | 36 | 10 | 1.6 | 0.0007 | 0.022 |
| BIOCARTA_INTRINSIC_PATHWAY | 23 | 8 | 2 | 0.0005 | 0.022 |
| KEGG_PYRIMIDINE_METABOLISM | 98 | 19 | 1.2 | 0.0006 | 0.022 |
PSD3,HLA-C,HLA-B,HLA-G,HLA-F,ARRB2,ARFGAP3,ERBB3,ASAP2,ASAP1,ASAP3,SNF8,AP2M1,GIT1,PARD6B,KDR,RABEP1,PIP4K2B,CLTA,VPS37A,IL8RB,VPS37C,CDC42,BRD8,UNC45A,CXCR4,LDLRAP1,RAB11FIP5,RAB11FIP2,ADRB1,CHMP1B,IGF1R,SH3GLB1,IL2RG,EHD2,STAMBP,FAM125B,DNM3,VPS28
GJA1,ACTG1,DES,ACTN1,CACNB2,ITGB3,TCF7L2,ITGB1,ITGB7,CACNA2D1,TCF7,ITGA1,ITGA3,LAMA2,ITGA9,DSG2,ITGA8,ITGA7
ITGB1,PXN,CDC42,NRG2,ITGA1,DVL1,LAMA2,MAPK1,PSG1,MAPK8
F12,F10,COL4A6,COL4A5,PROC,FGG,F5,FGA
NP,NME7,NME6,NME5,PNPT1,ZNRD1,TK1,DUT,NT5M,NT5E,POLR1D,POLR1B,UMPS,POLR2F,POLR2K,UCK2,POLR3H,POLR3A,POLR3B
Table 4. Get Full Table Top Pathways enriched with marker genes of gene expression subtype 3 . Nof Genes : No. of genes in this pathway. Nof Marker : No. of marker genes of gene expression subtype 3 in this pathway. Enrichment , P value and Q value : See Methods & Data below. Marker Gene in Pathway: markers of gene expression subtype 3 in this pathway
| Pathway | Nof Genes | Nof Marker | Enrichment | P value | Q value |
|---|---|---|---|---|---|
| KEGG_WNT_SIGNALING_PATHWAY | 151 | 35 | 1.4 | 0 | 0 |
| KEGG_PATHWAYS_IN_CANCER | 328 | 58 | 1 | 0 | 0 |
| KEGG_BASAL_CELL_CARCINOMA | 55 | 19 | 2 | 0 | 0 |
| BIOCARTA_G1_PATHWAY | 28 | 12 | 2.3 | 0 | 0.0001 |
| KEGG_MELANOGENESIS | 102 | 25 | 1.5 | 0 | 0.0001 |
PPP2R5A,BTRC,WNT3A,MMP7,PRKX,WNT3,CUL1,WNT10A,ROCK1,WNT5A,DAAM2,RAC1,AXIN1,SMAD4,SMAD3,SFRP2,PRICKLE2,LEF1,SUMO1,CCND1,TCF7L2,TCF7L1,NFAT5,CSNK1A1,FZD9,GPI,FZD4,FZD7,WNT2B,FZD6,FZD10,WNT7B,GSK3B,TBL1X,WNT7A
FAS,WNT10A,RXRG,MECOM,LAMC2,RAC1,FH,TGFBR2,SMAD4,SMAD3,LAMA4,LAMA3,FGF8,NFKB1,PTEN,TGFA,CCND1,SUFU,FZD9,FZD4,FZD7,FZD6,WNT7B,GSK3B,WNT7A,FGF19,WNT3A,TGFB3,FGF13,MMP2,MMP1,TGFB1,WNT3,CDKN2B,CSF3R,WNT5A,ITGB1,AXIN1,ITGA2,BRCA2,CDKN1B,HDAC1,ABL1,E2F1,HSP90AB1,AKT3,LEF1,CDK4,SMO,NCOA4,DCC,CKS1B,TCF7L2,TCF7L1,MSH6,WNT2B,FZD10,BAX
WNT3A,WNT3,WNT10A,LEF1,SMO,WNT5A,TCF7L2,TCF7L1,SUFU,AXIN1,FZD9,FZD4,FZD7,WNT2B,FZD6,WNT7B,FZD10,GSK3B,WNT7A
E2F1,TGFB3,TGFB1,CDKN2B,SMAD4,SMAD3,CDK4,CCND1,CDKN1B,HDAC1,GSK3B,ABL1
ADCY3,ADCY4,ADCY5,WNT3A,PRKX,WNT3,CREB3L1,CREB3L4,WNT10A,WNT5A,GNAI1,LEF1,TCF7L2,TCF7L1,CALML3,FZD9,FZD4,FZD7,FZD6,WNT2B,WNT7B,FZD10,GSK3B,WNT7A,CALM1
Let genes with copy number alteration or mutation be query genes. Let marker genes of specific identified subtypes be query genes. The Enrichment is calculated as:
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Enrichment = log2 (# of query genes in the pathway/# No of query genes) - log2 (# of genes in the pathway/# of human genes)
The statistical signficance of the pathways that are enriched with genes with copy number alteration or mutation, and the pathways that are enriched with markers genes of specific identified subtypes is measured by P value.
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P value = Fisher exact P value
The Q value is for adjusting P value for multiple testing. A public available R package is used to calculate the Q value.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.