| GDAC_TopgenesforCluster1 expfile | file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/HNSC/1227650/0.mRNAseq_preprocessor.Finished/HNSC.mRNAseq_RPKM_tmm_log2.txt |
| GDAC_TopgenesforCluster1 selectedgenes | 1500 |
| GDAC_NmfConsensusClustering2 k int | 2 |
| GDAC_NmfConsensusClustering2 k final | 8 |
| GDAC_CNMFselectcluster3 output | HNSC |
| GDAC_CNMFselectcluster3 inputallexp | file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/HNSC/1227650/0.mRNAseq_preprocessor.Finished/HNSC.mRNAseq_RPKM_tmm_log2.txt |
| GDAC_CnmfReports4 report | mRNAseq |
| Execution Times: | |
| Submitted: | 18:46:52 14-05-12 |
| Completed: | |
| Elapsed: | 00 hrs 21 mins 45 secs |
step 1. GDAC_TopgenesforCluster [id: 91002] This module can be used to selecte top genes with most variation, convert file to gct format, as well as imput missing value
urn:lsid:broadinstitute.org:cancer.genome.analysis:00205:20
| expfile | file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/HNSC/1227650/0.mRNAseq_preprocessor.Finished/HNSC.mRNAseq_RPKM_tmm_log2.txt |
| selectedgenes | 1500 |
| outputprefix | outputprefix |
| Output Files: | |
| .lsf.out | |
| outputprefix.expclu.gct | |
| stdout.txt | |
| Execution Times: | |
| Submitted: | 18:46:52 14-05-12 |
| Completed: | 18:48:59 14-05-12 |
| Elapsed: | 00 hrs 02 mins 07 secs |
step 2. GDAC_NmfConsensusClustering [id: 91003] Non-negative Matrix Factorization (NMF) Consensus Clustering, http://www.broadinstitute.org/cgi-bin/cancer/publications/pub_paper.cgi?mode=view&paper_id=89
urn:lsid:broadinstitute.org:cancer.genome.analysis:00379:38
| expfile | outputprefix.expclu.gct |
| k int | 2 |
| k final | 8 |
| outputprefix | cnmf |
| Execution Times: | |
| Submitted: | 18:46:52 14-05-12 |
| Completed: | 19:05:29 14-05-12 |
| Elapsed: | 00 hrs 18 mins 36 secs |
step 3. GDAC_CNMFselectcluster [id: 91004] selecte best cluster
urn:lsid:broadinstitute.org:cancer.genome.analysis:00226:24
| measure | Pearson |
| inputexp | outputprefix.expclu.gct |
| clumembership | cnmf.membership.txt |
| output | HNSC |
| inputallexp | file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/HNSC/1227650/0.mRNAseq_preprocessor.Finished/HNSC.mRNAseq_RPKM_tmm_log2.txt |
| cophenetic | cnmf.cophenetic.coefficient.txt |
| Output Files: | |
| HNSC.silfig.png | |
| HNSC.bestclus.txt | |
| HNSC.cormatrix.png | |
| HNSC.subclassmarkers.txt | |
| HNSC.geneheatmap.png | |
| HNSC.seclectedSubclassmarkers.txt | |
| HNSC.geneheatmaptopgenes.png | |
| .lsf.out | |
| stdout.txt | |
| Execution Times: | |
| Submitted: | 18:46:52 14-05-12 |
| Completed: | 19:08:05 14-05-12 |
| Elapsed: | 00 hrs 21 mins 12 secs |
step 4. GDAC_CnmfReports [id: 91005]
urn:lsid:broadinstitute.org:cancer.genome.analysis:00414:10
| kclus | HNSC.silfig.png |
| markers | HNSC.subclassmarkers.txt |
| bestclu | HNSC.bestclus.txt |
| allcluster | cnmf.membership.txt |
| cormatrix | HNSC.cormatrix.png |
| file gif 2 | cnmf.consensus.plot.k2.png |
| file gif 3 | cnmf.consensus.plot.k3.png |
| file gif 4 | cnmf.consensus.plot.k4.png |
| file gif 5 | cnmf.consensus.plot.k5.png |
| file gif 6 | cnmf.consensus.plot.k6.png |
| file gif 7 | cnmf.consensus.plot.k7.png |
| file gif 8 | cnmf.consensus.plot.k8.png |
| expdata | outputprefix.expclu.gct |
| markersP | HNSC.seclectedSubclassmarkers.txt |
| heatmap | HNSC.geneheatmap.png |
| heatmapall | HNSC.geneheatmaptopgenes.png |
| report | mRNAseq |
| Output Files: | |
| nozzle.RData | |
| nozzle.html | |
| .lsf.out | |
| stdout.txt | |
| Execution Times: | |
| Submitted: | 18:46:52 14-05-12 |
| Completed: | 19:08:35 14-05-12 |
| Elapsed: | 00 hrs 21 mins 42 secs |
