GDAC_CnmfIntegratedPipeline Execution Log 12:51 PM Thu Nov 8, '12

Running as user: cgaadm_deadline

GDAC_TopgenesforCluster1 expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/COAD/2008294/0.mRNAseq_preprocessor.Finished/COAD.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_TopgenesforCluster1 selectedgenes 1500
GDAC_NmfConsensusClustering2 k int 2
GDAC_NmfConsensusClustering2 k final 10
GDAC_CNMFselectcluster3 output COAD
GDAC_CNMFselectcluster3 inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/COAD/2008294/0.mRNAseq_preprocessor.Finished/COAD.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_CnmfReports4 report mRNAseq
Execution Times:
Submitted: 12:51:18 08-11-12
Completed:
Elapsed: 00 hrs 30 mins 25 secs

step 1. GDAC_TopgenesforCluster [id: 222435] This module can be used to selecte top genes with most variation, convert file to gct format, as well as imput missing value

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00205:33
expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/COAD/2008294/0.mRNAseq_preprocessor.Finished/COAD.uncv2.mRNAseq_RSEM_normalized_log2.txt
selectedgenes 1500
outputprefix outputprefix
Output Files:
 .lsf.out
 outputprefix.expclu.gct
 stdout.txt
Execution Times:
Submitted: 12:51:18 08-11-12
Completed: 12:53:26 08-11-12
Elapsed: 00 hrs 02 mins 08 secs

step 2. GDAC_NmfConsensusClustering [id: 222436] Non-negative Matrix Factorization (NMF) Consensus Clustering, http://www.broadinstitute.org/cgi-bin/cancer/publications/pub_paper.cgi?mode=view&paper_id=89

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00379:39
expfile outputprefix.expclu.gct
k int 2
k final 10
outputprefix cnmf
Output Files:
 cnmf.normalized.gct
 cnmf.params.txt
 cnmf.consensus.all.k.plot.png
 cnmf.consensus.plot.k2.png
 cnmf.consensus.plot.k3.png
 cnmf.consensus.plot.k4.png
 cnmf.consensus.plot.k5.png
 cnmf.consensus.plot.k6.png
 cnmf.consensus.plot.k7.png
 cnmf.consensus.plot.k8.png
 cnmf.consensus.plot.k9.png
 cnmf.cophenetic.coefficient.txt
 cnmf.membership.txt
 cnmf.cophenetic.coefficient.png
 cnmf.consensus.plot.k10.png
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 12:51:18 08-11-12
Completed: 13:18:16 08-11-12
Elapsed: 00 hrs 26 mins 57 secs

step 3. GDAC_CNMFselectcluster [id: 222437] selecte best cluster

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00226:33
measure Pearson
inputexp outputprefix.expclu.gct
clumembership cnmf.membership.txt
output COAD
inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/COAD/2008294/0.mRNAseq_preprocessor.Finished/COAD.uncv2.mRNAseq_RSEM_normalized_log2.txt
cophenetic cnmf.cophenetic.coefficient.txt
Output Files:
 COAD.silfig.png
 COAD.bestclus.txt
 COAD.cormatrix.png
 COAD.subclassmarkers.txt
 COAD.selectmarker.txt
 COAD.geneheatmap.png
 COAD.geneheatmaptopgenes.png
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 12:51:18 08-11-12
Completed: 13:21:20 08-11-12
Elapsed: 00 hrs 30 mins 02 secs

step 4. GDAC_CnmfReports [id: 222438]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00414:22
kclus COAD.silfig.png
markers COAD.subclassmarkers.txt
bestclu COAD.bestclus.txt
allcluster cnmf.membership.txt
cormatrix COAD.cormatrix.png
file gif 2 cnmf.consensus.plot.k2.png
file gif 3 cnmf.consensus.plot.k3.png
file gif 4 cnmf.consensus.plot.k4.png
file gif 5 cnmf.consensus.plot.k5.png
file gif 6 cnmf.consensus.plot.k6.png
file gif 7 cnmf.consensus.plot.k7.png
file gif 8 cnmf.consensus.plot.k8.png
expdata outputprefix.expclu.gct
markersP COAD.selectmarker.txt
heatmap COAD.geneheatmap.png
heatmapall COAD.geneheatmaptopgenes.png
report mRNAseq
Output Files:
 nozzle.html
 .lsf.out
 nozzle.RData
 stdout.txt
Execution Times:
Submitted: 12:51:18 08-11-12
Completed: 13:21:40 08-11-12
Elapsed: 00 hrs 30 mins 21 secs