This report serves to describe the mutational landscape and properties of a given individual set, as well as rank genes and genesets according to mutational significance. MutSig vS2N was used to generate the results found in this report.
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Working with individual set: THCA-TP
The input for this pipeline is a set of individuals with the following files associated for each:
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An annotated .maf file describing the mutations called for the respective individual, and their properties.
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A .wig file that contains information about the coverage of the sample.
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MAF used for this analysis:THCA-TP.final_analysis_set.maf
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Significantly mutated genes (q ≤ 0.1): 29
Column Descriptions:
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N = number of sequenced bases in this gene across the individual set
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nnon = number of (nonsilent) mutations in this gene across the individual set
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nnull = number of (nonsilent) null mutations in this gene across the individual set
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nflank = number of noncoding mutations from this gene's flanking region, across the individual set
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nsil = number of silent mutations in this gene across the individual set
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p = p-value (overall)
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q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)
Table 1. Get Full Table A Ranked List of Significantly Mutated Genes. Number of significant genes found: 29. Number of genes displayed: 35. Click on a gene name to display its stick figure depicting the distribution of mutations and mutation types across the chosen gene (this feature may not be available for all significant genes).
| gene | N | nflank | nsil | nnon | nnull | p | q |
|---|---|---|---|---|---|---|---|
| BRAF | 84303 | 0 | 1 | 183 | 0 | 0 | 0 |
| NRAS | 23790 | 0 | 0 | 26 | 0 | 0 | 0 |
| HRAS | 22597 | 0 | 0 | 12 | 0 | 0 | 0 |
| EMG1 | 30474 | 0 | 0 | 6 | 6 | 0 | 0 |
| TG | 318775 | 0 | 3 | 16 | 10 | 6.8e-126 | 2.6e-122 |
| PRB2 | 22808 | 0 | 1 | 6 | 3 | 1.9e-66 | 5.9e-63 |
| EIF1AX | 18088 | 0 | 0 | 6 | 1 | 3.6e-62 | 9.6e-59 |
| PTTG1IP | 17442 | 0 | 0 | 4 | 4 | 1.4e-48 | 3.2e-45 |
| MUC7 | 33157 | 0 | 1 | 5 | 1 | 1.1e-41 | 2.3e-38 |
| ZNF845 | 77648 | 0 | 0 | 6 | 0 | 1e-34 | 1.9e-31 |
| RPTN | 83868 | 0 | 0 | 8 | 7 | 5.6e-31 | 9.5e-28 |
| PPM1D | 56848 | 0 | 0 | 5 | 4 | 1.3e-24 | 2e-21 |
| TROAP | 68476 | 0 | 0 | 5 | 0 | 1.4e-20 | 2.1e-17 |
| PRG4 | 148455 | 0 | 1 | 7 | 3 | 2.7e-17 | 3.6e-14 |
| FAM47C | 90354 | 0 | 3 | 5 | 0 | 9.2e-16 | 1.1e-12 |
| ARID1B | 175824 | 0 | 0 | 6 | 2 | 9.7e-16 | 1.1e-12 |
| ZNF799 | 73433 | 0 | 1 | 5 | 0 | 1.2e-15 | 1.3e-12 |
| DNMT3A | 91620 | 0 | 0 | 5 | 4 | 1.5e-15 | 1.6e-12 |
| GPR44 | 14759 | 0 | 0 | 4 | 4 | 2.6e-15 | 2.6e-12 |
| R3HDM2 | 59828 | 0 | 0 | 4 | 4 | 9.8e-15 | 9.2e-12 |
| CCDC15 | 86358 | 0 | 1 | 5 | 0 | 3.3e-13 | 3e-10 |
| ZNF780A | 77369 | 0 | 1 | 4 | 0 | 4.1e-11 | 3.5e-08 |
| MLL3 | 523557 | 0 | 0 | 8 | 4 | 4.7e-11 | 3.9e-08 |
| ZFHX3 | 379960 | 0 | 2 | 10 | 4 | 5e-11 | 3.9e-08 |
| DICER1 | 232461 | 0 | 0 | 5 | 2 | 1.4e-06 | 0.0011 |
| COL5A3 | 116788 | 0 | 0 | 6 | 2 | 1.9e-06 | 0.0014 |
| ZNF208 | 160969 | 1 | 0 | 4 | 0 | 4.4e-06 | 0.003 |
| CD209 | 45543 | 0 | 1 | 4 | 0 | 0.00014 | 0.093 |
| CHD2 | 219627 | 0 | 0 | 4 | 4 | 0.00015 | 0.098 |
| ANK1 | 210075 | 0 | 0 | 4 | 0 | 0.00019 | 0.12 |
| ATM | 388272 | 0 | 0 | 5 | 2 | 0.00032 | 0.19 |
| PKHD1L1 | 454474 | 0 | 0 | 5 | 4 | 0.001 | 0.6 |
| ACRC | 65819 | 0 | 0 | 4 | 0 | 0.0021 | 1 |
| DNAH2 | 521223 | 0 | 0 | 5 | 0 | 0.0025 | 1 |
| PRDM9 | 98838 | 0 | 3 | 6 | 1 | 0.0026 | 1 |
Figure S1. This figure depicts the distribution of mutations and mutation types across the BRAF significant gene.
Figure S2. This figure depicts the distribution of mutations and mutation types across the NRAS significant gene.
Figure S3. This figure depicts the distribution of mutations and mutation types across the HRAS significant gene.
Figure S4. This figure depicts the distribution of mutations and mutation types across the EMG1 significant gene.
Figure S5. This figure depicts the distribution of mutations and mutation types across the TG significant gene.
Figure S6. This figure depicts the distribution of mutations and mutation types across the EIF1AX significant gene.
Figure S7. This figure depicts the distribution of mutations and mutation types across the PTTG1IP significant gene.
Figure S8. This figure depicts the distribution of mutations and mutation types across the MUC7 significant gene.
Figure S9. This figure depicts the distribution of mutations and mutation types across the ZNF845 significant gene.
Figure S10. This figure depicts the distribution of mutations and mutation types across the RPTN significant gene.
Figure S11. This figure depicts the distribution of mutations and mutation types across the PPM1D significant gene.
Figure S12. This figure depicts the distribution of mutations and mutation types across the TROAP significant gene.
Figure S13. This figure depicts the distribution of mutations and mutation types across the PRG4 significant gene.
Figure S14. This figure depicts the distribution of mutations and mutation types across the FAM47C significant gene.
Figure S15. This figure depicts the distribution of mutations and mutation types across the ARID1B significant gene.
Figure S16. This figure depicts the distribution of mutations and mutation types across the ZNF799 significant gene.
Figure S17. This figure depicts the distribution of mutations and mutation types across the DNMT3A significant gene.
Figure S18. This figure depicts the distribution of mutations and mutation types across the GPR44 significant gene.
Figure S19. This figure depicts the distribution of mutations and mutation types across the R3HDM2 significant gene.
Figure S20. This figure depicts the distribution of mutations and mutation types across the CCDC15 significant gene.
Figure S21. This figure depicts the distribution of mutations and mutation types across the ZNF780A significant gene.
Figure S22. This figure depicts the distribution of mutations and mutation types across the MLL3 significant gene.
Figure S23. This figure depicts the distribution of mutations and mutation types across the ZFHX3 significant gene.
Figure S24. This figure depicts the distribution of mutations and mutation types across the DICER1 significant gene.
Figure S25. This figure depicts the distribution of mutations and mutation types across the COL5A3 significant gene.
Figure S26. This figure depicts the distribution of mutations and mutation types across the CD209 significant gene.
In brief, we tabulate the number of mutations and the number of covered bases for each gene. The counts are broken down by mutation context category: four context categories that are discovered by MutSig, and one for indel and 'null' mutations, which include indels, nonsense mutations, splice-site mutations, and non-stop (read-through) mutations. For each gene, we calculate the probability of seeing the observed constellation of mutations, i.e. the product P1 x P2 x ... x Pm, or a more extreme one, given the background mutation rates calculated across the dataset. [1]