GDAC_RNAseq_ConsensusClusteringPipeline Execution Log 3:57 PM Thu Jan 10, '13

Running as user: cgaadm_deadline

GDAC_TopgenesforCluster1 expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/miRseq_preprocessor/THCA-TP/2284142/0.miRseq_preprocessor.Finished/THCA-TP.miRseq_RPKM_log2.txt
GDAC_TopgenesforCluster1 selectedgenes 150
GDAC_ConsensusClustering2 clustering algorithm HIERARCHICAL
GDAC_ConsensusClustering2 cluster by
GDAC_ConsensusClustering2 distance measure PEARSON
GDAC_ConsensusClustering2 normalize type
GDAC_selectBestcluster3 measure Pearson
GDAC_selectBestcluster3 output THCA-TP
GDAC_selectBestcluster3 inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/miRseq_preprocessor/THCA-TP/2284142/0.miRseq_preprocessor.Finished/THCA-TP.miRseq_RPKM_log2.txt
GDAC_CnmfReports4 report hclumiRseq
Execution Times:
Submitted: 15:57:32 10-01-13
Completed:
Elapsed: 00 hrs 07 mins 24 secs

step 1. GDAC_TopgenesforCluster [id: 286336] This module can be used to selecte top genes with most variation, convert file to gct format, as well as imput missing value

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00205:29
expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/miRseq_preprocessor/THCA-TP/2284142/0.miRseq_preprocessor.Finished/THCA-TP.miRseq_RPKM_log2.txt
selectedgenes 150
outputprefix outputprefix
Output Files:
 .lsf.out
 outputprefix.expclu.gct
 stdout.txt
Execution Times:
Submitted: 15:57:32 10-01-13
Completed: 15:58:25 10-01-13
Elapsed: 00 hrs 00 mins 53 secs

step 2. GDAC_ConsensusClustering [id: 286337] Resampling-based clustering method

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00158:6
input filename outputprefix.expclu.gct
kmax 8
resampling iterations 20
seed value 12345
clustering algorithm HIERARCHICAL
cluster by
distance measure PEARSON
resample subsample
merge type average
descent iterations 2000
output stub <input.filename_basename>
normalize type
normalization iterations 0
create heat map -p
heat map size 2
Output Files:
 outputprefix.expclu.sub278.2.gct
 outputprefix.expclu.sub278.clsdist
 outputprefix.expclu.sub278.stdev
 outputprefix.expclu.sub278.4.gct
 outputprefix.expclu.sub278.3.gct
 outputprefix.expclu.sub278.5.gct
 outputprefix.expclu.sub278.6.gct
 outputprefix.expclu.sub278.8.gct
 outputprefix.expclu.sub278.7.gct
 outputprefix.expclu.sub278.3.clu
 outputprefix.expclu.sub278.2.clu
 outputprefix.expclu.sub278.4.clu
 outputprefix.expclu.sub278.6.clu
 outputprefix.expclu.sub278.5.clu
 outputprefix.expclu.sub278.7.clu
 outputprefix.expclu.sub278.8.clu
 outputprefix.expclu.sub278.statistics.pdf
 outputprefix.expclu.sub278.srt.2.gct
 outputprefix.expclu.sub278.srt.3.gif
 outputprefix.expclu.sub278.srt.3.gct
 outputprefix.expclu.sub278.srt.4.gif
 outputprefix.expclu.sub278.srt.4.gct
 outputprefix.expclu.sub278.srt.5.gif
 outputprefix.expclu.sub278.srt.5.gct
 outputprefix.expclu.sub278.srt.2.gif
 outputprefix.expclu.sub278.srt.6.gif
 outputprefix.expclu.sub278.srt.6.gct
 outputprefix.expclu.sub278.srt.7.gct
 outputprefix.expclu.sub278.srt.7.gif
 outputprefix.expclu.sub278.srt.8.gct
 outputprefix.expclu.sub278.srt.8.gif
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 15:57:32 10-01-13
Completed: 16:02:25 10-01-13
Elapsed: 00 hrs 04 mins 52 secs

step 3. GDAC_selectBestcluster [id: 286338]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00207:32
measure Pearson
inputexp outputprefix.expclu.gct
output THCA-TP
file clu 2 outputprefix.expclu.sub278.2.clu
file clu 3 outputprefix.expclu.sub278.3.clu
file clu 4 outputprefix.expclu.sub278.4.clu
file clu 5 outputprefix.expclu.sub278.5.clu
file clu 6 outputprefix.expclu.sub278.6.clu
file clu 7 outputprefix.expclu.sub278.7.clu
file clu 8 outputprefix.expclu.sub278.8.clu
inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/miRseq_preprocessor/THCA-TP/2284142/0.miRseq_preprocessor.Finished/THCA-TP.miRseq_RPKM_log2.txt
Output Files:
 THCA-TP.silfig.png
 THCA-TP.allclusters.txt
 THCA-TP.bestclus.txt
 THCA-TP.cormatrix.png
 THCA-TP.subclassmarkers.txt
 THCA-TP.seclectedSubclassmarkers.txt
 THCA-TP.geneheatmap.png
 .lsf.out
 THCA-TP.geneheatmaptopgenes.png
 stdout.txt
Execution Times:
Submitted: 15:57:33 10-01-13
Completed: 16:03:06 10-01-13
Elapsed: 00 hrs 05 mins 33 secs

step 4. GDAC_CnmfReports [id: 286339]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00414:21