Cervical Squamous Cell Carcinoma: Mutation Analysis (MutSig v1.5)
(primary solid tumor cohort)
Maintained by Dan DiCara (Broad Institute)
Overview
Introduction

This report serves to describe the mutational landscape and properties of a given individual set, as well as rank genes and genesets according to mutational significance. MutSig v1.5 was used to generate the results found in this report.

  • Working with individual set: CESC-TP

  • Number of patients in set: 39

Input

The input for this pipeline is a set of individuals with the following files associated for each:

  1. An annotated .maf file describing the mutations called for the respective individual, and their properties.

  2. A .wig file that contains information about the coverage of the sample.

Summary

  • MAF used for this analysis:CESC-TP.final_analysis_set.maf

  • Significantly mutated genes (q ≤ 0.1): 5

  • Mutations seen in COSMIC: 35

  • Significantly mutated genes in COSMIC territory: 1

  • Genes with clustered mutations (≤ 3 aa apart): 76

  • Significantly mutated genesets: 0

  • Significantly mutated genesets: (excluding sig. mutated genes):0

Mutation Preprocessing

  • Read 39 MAFs of type "Broad"

  • Total number of mutations in input MAFs: 10020

  • After removing 5 mutations outside chr1-24: 10015

  • After removing 261 blacklisted mutations: 9754

  • After removing 617 noncoding mutations: 9137

Mutation Filtering

  • Number of mutations before filtering: 9137

  • After removing 127 mutations outside gene set: 9010

  • After removing 21 mutations outside category set: 8989

Results
Breakdown of Mutations by Type

Table 1.  Get Full Table Table representing breakdown of mutations by type.

type count
Frame_Shift_Del 114
Frame_Shift_Ins 65
In_Frame_Del 52
In_Frame_Ins 15
Missense_Mutation 5620
Nonsense_Mutation 474
Nonstop_Mutation 11
Silent 2532
Splice_Site 93
Translation_Start_Site 13
Total 8989
Breakdown of Mutation Rates by Category Type

Table 2.  Get Full Table A breakdown of mutation rates per category discovered for this individual set.

category n N rate rate_per_mb relative_rate exp_ns_s_ratio
Tp*C->(T/G) 3536 150569812 0.000023 23 4.1 3
(A/C/G)p*C->(T/G) 1136 425623126 2.7e-06 2.7 0.47 2.7
C->A 350 576192938 6.1e-07 0.61 0.11 4.4
A->mut 611 556925266 1.1e-06 1.1 0.19 3.9
indel+null 805 1133118204 7.1e-07 0.71 0.12 NaN
double_null 19 1133118204 1.7e-08 0.017 0.0029 NaN
Total 6457 1133118204 5.7e-06 5.7 1 3.5
Target Coverage for Each Individual

The x axis represents the samples. The y axis represents the exons, one row per exon, and they are sorted by average coverage across samples. For exons with exactly the same average coverage, they are sorted next by the %GC of the exon. (The secondary sort is especially useful for the zero-coverage exons at the bottom).

Figure 1. 

Distribution of Mutation Counts, Coverage, and Mutation Rates Across Samples

Figure 2.  Patients counts and rates file used to generate this plot: CESC-TP.patients.counts_and_rates.txt

CoMut Plot

Figure 3.  Get High-res Image The matrix in the center of the figure represents individual mutations in patient samples, color-coded by type of mutation, for the significantly mutated genes. The rate of synonymous and non-synonymous mutations is displayed at the top of the matrix. The barplot on the left of the matrix shows the number of mutations in each gene. The percentages represent the fraction of tumors with at least one mutation in the specified gene. The barplot to the right of the matrix displays the q-values for the most significantly mutated genes. The purple boxplots below the matrix (only displayed if required columns are present in the provided MAF) represent the distributions of allelic fractions observed in each sample. The plot at the bottom represents the base substitution distribution of individual samples, using the same categories that were used to calculate significance.

Significantly Mutated Genes

Column Descriptions:

  • N = number of sequenced bases in this gene across the individual set

  • n = number of (nonsilent) mutations in this gene across the individual set

  • npat = number of patients (individuals) with at least one nonsilent mutation

  • nsite = number of unique sites having a non-silent mutation

  • nsil = number of silent mutations in this gene across the individual set

  • n1 = number of nonsilent mutations of type: Tp*C->(T/G)

  • n2 = number of nonsilent mutations of type: (A/C/G)p*C->(T/G)

  • n3 = number of nonsilent mutations of type: C->A

  • n4 = number of nonsilent mutations of type: A->mut

  • n5 = number of nonsilent mutations of type: indel+null

  • n6 = number of nonsilent mutations of type: double_null

  • p_ns_s = p-value for the observed nonsilent/silent ratio being elevated in this gene

  • p = p-value (overall)

  • q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)

Table 3.  Get Full Table A Ranked List of Significantly Mutated Genes. Number of significant genes found: 5. Number of genes displayed: 35. Click on a gene name to display its stick figure depicting the distribution of mutations and mutation types across the chosen gene (this feature may not be available for all significant genes).

rank gene description N n npat nsite nsil n1 n2 n3 n4 n5 n6 p_ns_s p q
1 PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide 126650 11 9 4 0 10 0 0 1 0 0 0.12 3.4e-08 0.0006
2 NFE2L2 nuclear factor (erythroid-derived 2)-like 2 69545 7 6 6 0 4 0 0 1 2 0 0.34 2.6e-06 0.024
3 PRB2 proline-rich protein BstNI subfamily 2 49240 6 4 5 0 0 0 0 1 5 0 0.52 1e-05 0.063
4 CDC27 cell division cycle 27 homolog (S. cerevisiae) 97176 7 5 4 0 0 0 1 3 3 0 0.37 2e-05 0.09
5 ARID1A AT rich interactive domain 1A (SWI-like) 226053 7 6 6 0 1 1 0 0 3 2 0.29 0.000025 0.09
6 PTH2 parathyroid hormone 2 8040 2 2 2 0 1 0 1 0 0 0 0.38 5e-05 0.15
7 SSX7 synovial sarcoma, X breakpoint 7 23049 5 3 4 0 0 2 1 2 0 0 0.34 0.000077 0.18
8 PTEN phosphatase and tensin homolog (mutated in multiple advanced cancers 1) 45935 3 3 3 0 0 0 0 0 3 0 0.59 0.000082 0.18
9 AQP2 aquaporin 2 (collecting duct) 29453 3 3 3 0 1 1 0 1 0 0 0.33 0.000096 0.19
10 TMCC1 transmembrane and coiled-coil domain family 1 77000 4 4 3 0 0 0 1 0 3 0 0.82 0.00013 0.21
11 LYZL1 lysozyme-like 1 22057 3 3 3 0 1 1 0 0 1 0 0.36 0.00013 0.21
12 PLAC4 placenta-specific 4 7160 2 2 2 0 0 0 0 0 1 1 1 0.00014 0.21
13 OR5H6 olfactory receptor, family 5, subfamily H, member 6 38003 3 3 3 0 1 0 0 1 1 0 0.39 0.00016 0.22
14 ZNF43 zinc finger protein 43 95053 4 4 4 0 1 0 1 1 1 0 0.58 0.00017 0.22
15 ERP27 endoplasmic reticulum protein 27 kDa 32955 3 3 3 0 0 2 0 1 0 0 0.46 0.0002 0.24
16 TMEM51 transmembrane protein 51 29872 3 3 3 0 1 1 0 1 0 0 0.4 0.00032 0.33
17 FCRL1 Fc receptor-like 1 53853 3 3 3 0 1 0 0 2 0 0 0.45 0.00037 0.33
18 PRG4 proteoglycan 4 166081 14 5 11 0 2 1 1 0 10 0 0.46 0.00038 0.33
19 TPTE transmembrane phosphatase with tensin homology 67719 3 3 3 0 1 0 0 1 1 0 0.62 0.00039 0.33
20 IQCG IQ motif containing G 53508 3 3 3 0 2 0 0 0 1 0 0.46 0.0004 0.33
21 DENND2C DENN/MADD domain containing 2C 104208 4 4 4 1 3 1 0 0 0 0 0.73 0.00042 0.33
22 TMCO2 transmembrane and coiled-coil domains 2 21723 2 2 2 0 1 0 0 0 1 0 0.79 0.00042 0.33
23 DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 39158 5 3 5 0 3 0 1 0 1 0 0.21 0.00042 0.33
24 ARHGAP6 Rho GTPase activating protein 6 97775 4 4 4 1 2 0 2 0 0 0 0.69 0.00044 0.33
25 NFYB nuclear transcription factor Y, beta 25367 2 2 1 0 0 0 0 0 2 0 0.55 0.00078 0.56
26 MMEL1 membrane metallo-endopeptidase-like 1 78452 4 4 4 0 2 2 0 0 0 0 0.21 0.0009 0.57
27 C11orf52 chromosome 11 open reading frame 52 14969 2 2 2 0 1 0 0 0 1 0 0.51 0.00092 0.57
28 KLHL1 kelch-like 1 (Drosophila) 86290 3 3 3 0 0 2 0 1 0 0 0.46 0.00093 0.57
29 MAGEC1 melanoma antigen family C, 1 133929 7 5 6 0 2 0 0 2 3 0 0.28 0.00098 0.57
30 IL28B interleukin 28B (interferon, lambda 3) 21576 2 2 2 0 0 1 0 1 0 0 0.57 0.001 0.57
31 PCSK4 proprotein convertase subtilisin/kexin type 4 50811 3 3 3 0 1 1 1 0 0 0 0.29 0.001 0.57
32 CSAG1 chondrosarcoma associated gene 1 9711 3 2 3 0 0 0 0 3 0 0 0.4 0.001 0.57
33 MAPK1 mitogen-activated protein kinase 1 38660 3 3 1 0 3 0 0 0 0 0 0.41 0.0011 0.58
34 ZFAND5 zinc finger, AN1-type domain 5 25726 2 2 2 0 0 1 1 0 0 0 0.74 0.0011 0.58
35 ZNF681 zinc finger protein 681 72584 3 3 3 0 1 0 1 1 0 0 0.6 0.0011 0.58
PIK3CA

Figure S1.  This figure depicts the distribution of mutations and mutation types across the PIK3CA significant gene.

NFE2L2

Figure S2.  This figure depicts the distribution of mutations and mutation types across the NFE2L2 significant gene.

CDC27

Figure S3.  This figure depicts the distribution of mutations and mutation types across the CDC27 significant gene.

ARID1A

Figure S4.  This figure depicts the distribution of mutations and mutation types across the ARID1A significant gene.

COSMIC analyses

In this analysis, COSMIC is used as a filter to increase power by restricting the territory of each gene. Cosmic version: v48.

Table 4.  Get Full Table Significantly mutated genes (COSMIC territory only). To access the database please go to: COSMIC. Number of significant genes found: 1. Number of genes displayed: 10

rank gene description n cos n_cos N_cos cos_ev p q
1 PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide 11 220 8 8580 3939 2.2e-13 9.8e-10
2 ATL2 atlastin GTPase 2 1 1 1 39 1 0.00022 0.17
3 EDA ectodysplasin A 1 1 1 39 1 0.00022 0.17
4 KIAA1024 KIAA1024 2 1 1 39 1 0.00022 0.17
5 MAPK11 mitogen-activated protein kinase 11 1 1 1 39 1 0.00022 0.17
6 SUV39H2 suppressor of variegation 3-9 homolog 2 (Drosophila) 1 1 1 39 2 0.00022 0.17
7 ALS2 amyotrophic lateral sclerosis 2 (juvenile) 2 3 1 117 1 0.00067 0.23
8 EPHA10 EPH receptor A10 1 3 1 117 1 0.00067 0.23
9 IFITM3 interferon induced transmembrane protein 3 (1-8U) 5 3 1 117 1 0.00067 0.23
10 ITIH5L inter-alpha (globulin) inhibitor H5-like 1 3 1 117 1 0.00067 0.23

Note:

n - number of (nonsilent) mutations in this gene across the individual set.

cos = number of unique mutated sites in this gene in COSMIC

n_cos = overlap between n and cos.

N_cos = number of individuals times cos.

cos_ev = total evidence: number of reports in COSMIC for mutations seen in this gene.

p = p-value for seeing the observed amount of overlap in this gene)

q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)

Clustered Mutations

Table 5.  Get Full Table Genes with Clustered Mutations

num gene desc n mindist nmuts0 nmuts3 nmuts12 npairs0 npairs3 npairs12
2854 PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide 11 0 14 24 24 14 24 24
4403 ZSCAN5A zinc finger and SCAN domain containing 5A 39 0 12 31 61 12 31 61
2624 OR1S2 olfactory receptor, family 1, subfamily S, member 2 33 0 11 27 39 11 27 39
2623 OR1S1 olfactory receptor, family 1, subfamily S, member 1 32 0 11 21 45 11 21 45
2196 MAGEA12 melanoma antigen family A, 12 29 0 10 26 44 10 26 44
3034 PRSS48 protease, serine, 48 16 0 6 22 54 6 22 54
2613 OR10G8 olfactory receptor, family 10, subfamily G, member 8 18 0 5 5 13 5 5 13
1773 IFITM2 interferon induced transmembrane protein 2 (1-8D) 9 0 3 3 5 3 3 5
2231 MAPK1 mitogen-activated protein kinase 1 3 0 3 3 3 3 3 3
4404 ZSCAN5B zinc finger and SCAN domain containing 5B 16 0 2 9 13 2 9 13

Note:

n - number of mutations in this gene in the individual set.

mindist - distance (in aa) between closest pair of mutations in this gene

npairs3 - how many pairs of mutations are within 3 aa of each other.

npairs12 - how many pairs of mutations are within 12 aa of each other.

Geneset Analyses

Table 6.  Get Full Table A Ranked List of Significantly Mutated Genesets. (Source: MSigDB GSEA Cannonical Pathway Set).Number of significant genesets found: 0. Number of genesets displayed: 10

rank geneset description genes N_genes mut_tally N n npat nsite nsil n1 n2 n3 n4 n5 n6 p_ns_s p q
1 PAR1PATHWAY Activated extracellular thrombin cleaves and activates the G-protein coupled receptors PAR1 and PAR4, which activate platelets. ADCY1, ARHA, ARHGEF1, F2, F2R, F2RL3, GNA12, GNA13, GNAI1, GNAQ, GNB1, GNGT1, MAP3K7, PIK3CA, PIK3R1, PLCB1, PPP1R12B, PRKCA, PRKCB1, PTK2B, ROCK1 19 ADCY1(1), ARHGEF1(1), F2R(1), F2RL3(2), GNA13(1), PIK3CA(11), PIK3R1(1), PPP1R12B(1), PRKCA(1), ROCK1(3) 1478575 23 13 16 1 16 3 1 2 0 1 0.011 0.00048 0.14
2 ACHPATHWAY Nicotinic acetylcholine receptors are ligand-gated ion channels that primarily mediate neuromuscular signaling and may inhibit neuronal apoptosis via the AKT pathway. AKT1, BAD, CHRNB1, CHRNG, FOXO3A, MUSK, PIK3CA, PIK3R1, PTK2, PTK2B, RAPSN, SRC, TERT, TNFSF6, YWHAH 13 CHRNG(1), MUSK(1), PIK3CA(11), PIK3R1(1), PTK2(1) 935251 15 11 8 1 11 0 1 2 1 0 0.053 0.0005 0.14
3 ERK5PATHWAY Signaling between a tissue and its innervating axon stimulates retrograde transport via Trk receptors, which activate Erk5, which induces transcription of anti-apoptotic factors. AKT1, CREB1, GRB2, HRAS, MAPK1, MAPK3, MAPK7, MEF2A, MEF2B, MEF2C, MEF2D, NTRK1, PIK3CA, PIK3R1, PLCG1, RPS6KA1, SHC1 17 MAPK1(3), MAPK7(1), MEF2C(1), PIK3CA(11), PIK3R1(1), RPS6KA1(2) 1088110 19 11 10 1 14 1 0 3 1 0 0.03 0.00078 0.14
4 GCRPATHWAY Corticosteroids activate the glucocorticoid receptor (GR), which inhibits NF-kB and activates Annexin-1, thus inhibiting the inflammatory response. ADRB2, AKT1, ANXA1, CALM1, CALM2, CALM3, CRN, GNAS, GNB1, GNGT1, HSPCA, NFKB1, NOS3, NPPA, NR3C1, PIK3CA, PIK3R1, RELA, SYT1 17 CALM1(1), CALM3(1), NPPA(1), PIK3CA(11), PIK3R1(1), SYT1(1) 1019231 16 11 9 0 12 1 1 2 0 0 0.018 0.0009 0.14
5 CTLA4PATHWAY T cell activation requires interaction with an antigen-MHC-I complex on an antigen-presenting cell (APC), as well as CD28 interaction with the APC's CD80 or 86. CD28, CD3D, CD3E, CD3G, CD3Z, CD80, CD86, CTLA4, GRB2, HLA-DRA, HLA-DRB1, ICOS, ICOSL, IL2, ITK, LCK, PIK3CA, PIK3R1, PTPN11, TRA@, TRB@ 17 CD86(1), ITK(1), PIK3CA(11), PIK3R1(1), PTPN11(1) 739491 15 9 8 0 12 1 0 2 0 0 0.018 0.0016 0.17
6 PTENPATHWAY PTEN suppresses AKT-induced cell proliferation and antagonizes the action of PI3K. AKT1, BCAR1, CDKN1B, FOXO3A, GRB2, ILK, ITGB1, MAPK1, MAPK3, PDK2, PDPK1, PIK3CA, PIK3R1, PTEN, PTK2, SHC1, SOS1, TNFSF6 16 MAPK1(3), PIK3CA(11), PIK3R1(1), PTEN(3), PTK2(1), SOS1(1) 1108069 20 11 11 0 15 0 0 2 3 0 0.0079 0.0017 0.17
7 ST_TYPE_I_INTERFERON_PATHWAY Type I interferon is an antiviral cytokine that induces a JAK-STAT type pathway leading to ISGF3 activation and a cellular antiviral response. IFNAR1, IFNB1, ISGF3G, JAK1, PTPRU, REG1A, STAT1, STAT2, TYK2 8 IFNAR1(2), PTPRU(1), REG1A(1), STAT1(3), TYK2(3) 723179 10 10 10 1 7 3 0 0 0 0 0.12 0.0019 0.17
8 IFNGPATHWAY IFN gamma signaling pathway IFNG, IFNGR1, IFNGR2, JAK1, JAK2, STAT1 6 IFNGR1(2), IFNGR2(2), JAK2(1), STAT1(3) 473117 8 7 8 1 4 1 0 0 3 0 0.48 0.0022 0.17
9 ARFPATHWAY Cyclin-dependent kinase inhibitor 2A is a tumor suppressor that induces G1 arrest and can activate the p53 pathway, leading to G2/M arrest. ABL1, CDKN2A, E2F1, MDM2, MYC, PIK3CA, PIK3R1, POLR1A, POLR1B, POLR1C, POLR1D, RAC1, RB1, TBX2, TP53, TWIST1 16 ABL1(1), PIK3CA(11), PIK3R1(1), POLR1A(2), POLR1C(2), TP53(2) 1168503 19 14 12 2 13 0 0 4 2 0 0.13 0.0032 0.2
10 PLCPATHWAY Phospholipase C hydrolyzes the membrane lipid PIP2 to DAG, which activates protein kinase C, and IP3, which causes calcium influx. AKT1, PIK3CA, PIK3R1, PLCB1, PLCG1, PRKCA, PRKCB1, VAV1 7 PIK3CA(11), PIK3R1(1), PRKCA(1) 739813 13 9 6 1 11 0 0 2 0 0 0.13 0.0032 0.2

Table 7.  Get Full Table A Ranked List of Significantly Mutated Genesets (Excluding Significantly Mutated Genes). Number of significant genesets found: 0. Number of genesets displayed: 10

rank geneset description genes N_genes mut_tally N n npat nsite nsil n1 n2 n3 n4 n5 n6 p_ns_s p q
1 ST_TYPE_I_INTERFERON_PATHWAY Type I interferon is an antiviral cytokine that induces a JAK-STAT type pathway leading to ISGF3 activation and a cellular antiviral response. IFNAR1, IFNB1, ISGF3G, JAK1, PTPRU, REG1A, STAT1, STAT2, TYK2 8 IFNAR1(2), PTPRU(1), REG1A(1), STAT1(3), TYK2(3) 723179 10 10 10 1 7 3 0 0 0 0 0.12 0.0019 0.69
2 IFNGPATHWAY IFN gamma signaling pathway IFNG, IFNGR1, IFNGR2, JAK1, JAK2, STAT1 6 IFNGR1(2), IFNGR2(2), JAK2(1), STAT1(3) 473117 8 7 8 1 4 1 0 0 3 0 0.48 0.0022 0.69
3 IFNAPATHWAY Interferon alpha, active in the immune response, binds to the IFN receptor and activates Jak1 and Tyk2, which phosphorylate Stat1 and Stat2. IFNA1, IFNAR1, IFNAR2, IFNB1, ISGF3G, JAK1, STAT1, STAT2, TYK2 8 IFNA1(1), IFNAR1(2), IFNAR2(1), STAT1(3), TYK2(3) 627650 10 9 10 1 9 1 0 0 0 0 0.14 0.011 1
4 FIBRINOLYSISPATHWAY Thrombin cleavage of fibrinogen results in rapid formation of fibrin threads that form a mesh to capture platelets and other blood cells into a clot. CPB2, F13A1, F2, F2R, FGA, FGB, FGG, PLAT, PLAU, PLG, SERPINB2, SERPINE1 12 CPB2(2), F2R(1), FGA(1), PLAU(1), PLG(2) 774038 7 7 7 1 3 1 1 2 0 0 0.43 0.019 1
5 ST_INTERFERON_GAMMA_PATHWAY The interferon gamma pathway resembles the JAK-STAT pathway and activates STAT transcription factors. CISH, IFNG, IFNGR1, JAK1, JAK2, PLA2G2A, PTPRU, REG1A, STAT1, STATIP1 9 IFNGR1(2), JAK2(1), PTPRU(1), REG1A(1), STAT1(3) 661911 8 7 8 1 4 3 0 0 1 0 0.27 0.02 1
6 NKTPATHWAY T cell differentiation into Th1 and Th2 cells occurs by differential chemokine receptor expression, which mediates tissue localization and immune response. CCL3, CCL4, CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CD28, CD4, CSF2, CXCR3, CXCR4, IFNG, IFNGR1, IFNGR2, IL12A, IL12B, IL12RB1, IL12RB2, IL18R1, IL2, IL4, IL4R, IL5, TGFB1, TGFB2, TGFB3, TNFSF5 28 CCL4(1), CCR7(1), IFNGR1(2), IFNGR2(2), IL12B(1), IL12RB1(1), TGFB3(2) 1166496 10 9 10 1 5 0 1 0 4 0 0.2 0.029 1
7 HSA00130_UBIQUINONE_BIOSYNTHESIS Genes involved in ubiquinone biosynthesis COQ2, COQ3, COQ5, COQ6, COQ7, ND1, ND2, ND3, ND4, ND4L, ND5, ND6, NDUFA12, NDUFA13, NDUFB11 8 COQ3(1), COQ5(1), COQ6(1) 232317 3 3 3 0 2 1 0 0 0 0 0.44 0.036 1
8 PAR1PATHWAY Activated extracellular thrombin cleaves and activates the G-protein coupled receptors PAR1 and PAR4, which activate platelets. ADCY1, ARHA, ARHGEF1, F2, F2R, F2RL3, GNA12, GNA13, GNAI1, GNAQ, GNB1, GNGT1, MAP3K7, PIK3CA, PIK3R1, PLCB1, PPP1R12B, PRKCA, PRKCB1, PTK2B, ROCK1 18 ADCY1(1), ARHGEF1(1), F2R(1), F2RL3(2), GNA13(1), PIK3R1(1), PPP1R12B(1), PRKCA(1), ROCK1(3) 1351925 12 9 12 1 6 3 1 1 0 1 0.13 0.038 1
9 TERTPATHWAY hTERC, the RNA subunit of telomerase, and hTERT, the catalytic protein subunit, are required for telomerase activity and are overexpressed in many cancers. HDAC1, MAX, MYC, SP1, SP3, TP53, WT1, ZNF42 7 MAX(1), SP3(1), TP53(2) 407974 4 4 4 0 2 1 0 0 1 0 0.33 0.039 1
10 ERBB4PATHWAY ErbB4 (aka HER4) is a receptor tyrosine kinase that binds neuregulins as well as members of the EGF family, which also target EGF receptors. ADAM17, ERBB4, NRG2, NRG3, PRKCA, PRKCB1, PSEN1 6 ADAM17(2), ERBB4(1), PRKCA(1), PSEN1(2) 515411 6 5 6 1 4 1 0 1 0 0 0.47 0.052 1
Methods & Data
Methods

In brief, we tabulate the number of mutations and the number of covered bases for each gene. The counts are broken down by mutation context category: four context categories that are discovered by MutSig, and one for indel and 'null' mutations, which include indels, nonsense mutations, splice-site mutations, and non-stop (read-through) mutations. For each gene, we calculate the probability of seeing the observed constellation of mutations, i.e. the product P1 x P2 x ... x Pm, or a more extreme one, given the background mutation rates calculated across the dataset. [1]

Download Results

This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.

References
[1] TCGA, Integrated genomic analyses of ovarian carcinoma, Nature 474:609 - 615 (2011)
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