This pipeline uses various statistical tests to identify mRNAs whose expression levels correlated to selected clinical features.
Testing the association between 18061 genes and 12 clinical features across 243 samples, statistically thresholded by Q value < 0.05, 10 clinical features related to at least one genes.
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82 genes correlated to 'PRIMARY.SITE.OF.DISEASE'.
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MTERF|7978 , CLDN18|51208 , ZNF12|7559 , C19ORF20|91978 , ACVR2B|93 , ...
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33 genes correlated to 'GENDER'.
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ZFY|7544 , XIST|7503 , KDM5D|8284 , RPS4Y1|6192 , PRKY|5616 , ...
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210 genes correlated to 'HISTOLOGICAL.TYPE'.
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USPL1|10208 , AQP3|360 , NR6A1|2649 , MRPS31|10240 , SLC25A36|55186 , ...
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6 genes correlated to 'PATHOLOGY.T'.
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FSTL3|10272 , RAB6B|51560 , IGLON5|402665 , GADD45B|4616 , TRPC1|7220 , ...
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41 genes correlated to 'PATHOLOGY.N'.
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HAP1|9001 , MAP6|4135 , ATP5B|506 , SUSD5|26032 , ZBTB20|26137 , ...
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12 genes correlated to 'PATHOLOGICSPREAD(M)'.
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TDRD12|91646 , RB1|5925 , FAM171A1|221061 , ZNF558|148156 , ZNF675|171392 , ...
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18 genes correlated to 'TUMOR.STAGE'.
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HAP1|9001 , CAMK2B|816 , TCHH|7062 , BEND3|57673 , UNG|7374 , ...
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15 genes correlated to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
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ATP5L2|267020 , C1ORF74|148304 , GAPDHS|26330 , CDH22|64405 , KLHDC8A|55220 , ...
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2 genes correlated to 'COMPLETENESS.OF.RESECTION'.
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ZNHIT2|741 , OVCH1|341350
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14 genes correlated to 'NUMBER.OF.LYMPH.NODES'.
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NIPAL4|348938 , CRLF1|9244 , AMIGO2|347902 , LOC100126784|100126784 , C5ORF23|79614 , ...
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No genes correlated to 'Time to Death', and 'AGE'.
Complete statistical result table is provided in Supplement Table 1
Table 1. Get Full Table This table shows the clinical features, statistical methods used, and the number of genes that are significantly associated with each clinical feature at Q value < 0.05.
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
---|---|---|---|---|---|---|
Time to Death | Cox regression test | N=0 | ||||
AGE | Spearman correlation test | N=0 | ||||
PRIMARY SITE OF DISEASE | t test | N=82 | rectum | N=53 | colon | N=29 |
GENDER | t test | N=33 | male | N=13 | female | N=20 |
HISTOLOGICAL TYPE | ANOVA test | N=210 | ||||
PATHOLOGY T | Spearman correlation test | N=6 | higher pT | N=6 | lower pT | N=0 |
PATHOLOGY N | Spearman correlation test | N=41 | higher pN | N=34 | lower pN | N=7 |
PATHOLOGICSPREAD(M) | ANOVA test | N=12 | ||||
TUMOR STAGE | Spearman correlation test | N=18 | higher stage | N=15 | lower stage | N=3 |
RADIATIONS RADIATION REGIMENINDICATION | t test | N=15 | yes | N=4 | no | N=11 |
COMPLETENESS OF RESECTION | ANOVA test | N=2 | ||||
NUMBER OF LYMPH NODES | Spearman correlation test | N=14 | higher number.of.lymph.nodes | N=12 | lower number.of.lymph.nodes | N=2 |
Table S1. Basic characteristics of clinical feature: 'Time to Death'
Time to Death | Duration (Months) | 0.1-129.1 (median=6.1) |
censored | N = 197 | |
death | N = 27 | |
Significant markers | N = 0 |
Table S2. Basic characteristics of clinical feature: 'AGE'
AGE | Mean (SD) | 64.97 (13) |
Significant markers | N = 0 |
Table S3. Basic characteristics of clinical feature: 'PRIMARY.SITE.OF.DISEASE'
PRIMARY.SITE.OF.DISEASE | Labels | N |
COLON | 192 | |
RECTUM | 49 | |
Significant markers | N = 82 | |
Higher in RECTUM | 53 | |
Higher in COLON | 29 |
Table S4. Get Full Table List of top 10 genes differentially expressed by 'PRIMARY.SITE.OF.DISEASE'
T(pos if higher in 'RECTUM') | ttestP | Q | AUC | |
---|---|---|---|---|
MTERF|7978 | 7.34 | 4.368e-12 | 7.89e-08 | 0.7176 |
CLDN18|51208 | -6.65 | 2.774e-10 | 5.01e-06 | 0.676 |
ZNF12|7559 | 7.04 | 3.543e-10 | 6.4e-06 | 0.772 |
C19ORF20|91978 | -7.03 | 6.574e-10 | 1.19e-05 | 0.7865 |
ACVR2B|93 | 6.62 | 1.39e-09 | 2.51e-05 | 0.7537 |
ZNF737|100129842 | 6.21 | 3.614e-09 | 6.53e-05 | 0.6783 |
ZNF470|388566 | 6.15 | 4.095e-09 | 7.39e-05 | 0.6642 |
IRF2BP2|359948 | 6.42 | 4.296e-09 | 7.76e-05 | 0.7379 |
ZNF628|89887 | -6.32 | 9.634e-09 | 0.000174 | 0.7516 |
SHROOM4|57477 | 6.08 | 1.093e-08 | 0.000197 | 0.7068 |
Figure S1. Get High-res Image As an example, this figure shows the association of MTERF|7978 to 'PRIMARY.SITE.OF.DISEASE'. P value = 4.37e-12 with T-test analysis.

Table S5. Basic characteristics of clinical feature: 'GENDER'
GENDER | Labels | N |
FEMALE | 115 | |
MALE | 128 | |
Significant markers | N = 33 | |
Higher in MALE | 13 | |
Higher in FEMALE | 20 |
Table S6. Get Full Table List of top 10 genes differentially expressed by 'GENDER'
T(pos if higher in 'MALE') | ttestP | Q | AUC | |
---|---|---|---|---|
ZFY|7544 | 49.76 | 2.759e-108 | 4.98e-104 | 0.9995 |
XIST|7503 | -43.26 | 3.441e-108 | 6.21e-104 | 0.9996 |
KDM5D|8284 | 61.45 | 1.296e-95 | 2.34e-91 | 1 |
RPS4Y1|6192 | 41.8 | 6.403e-91 | 1.16e-86 | 1 |
PRKY|5616 | 31.54 | 7.705e-84 | 1.39e-79 | 0.9958 |
EIF1AY|9086 | 46.79 | 4.691e-74 | 8.47e-70 | 1 |
DDX3Y|8653 | 52.66 | 6.485e-71 | 1.17e-66 | 1 |
USP9Y|8287 | 45.64 | 3.853e-65 | 6.95e-61 | 1 |
NLGN4Y|22829 | 35.24 | 9.159e-60 | 1.65e-55 | 0.9994 |
TSIX|9383 | -22.39 | 9.878e-53 | 1.78e-48 | 0.9989 |
Figure S2. Get High-res Image As an example, this figure shows the association of ZFY|7544 to 'GENDER'. P value = 2.76e-108 with T-test analysis.

Table S7. Basic characteristics of clinical feature: 'HISTOLOGICAL.TYPE'
HISTOLOGICAL.TYPE | Labels | N |
COLON ADENOCARCINOMA | 168 | |
COLON MUCINOUS ADENOCARCINOMA | 24 | |
RECTAL ADENOCARCINOMA | 47 | |
RECTAL MUCINOUS ADENOCARCINOMA | 2 | |
Significant markers | N = 210 |
Table S8. Get Full Table List of top 10 genes differentially expressed by 'HISTOLOGICAL.TYPE'
ANOVA_P | Q | |
---|---|---|
USPL1|10208 | 1.867e-10 | 3.37e-06 |
AQP3|360 | 2.608e-10 | 4.71e-06 |
NR6A1|2649 | 3.571e-10 | 6.45e-06 |
MRPS31|10240 | 3.899e-10 | 7.04e-06 |
SLC25A36|55186 | 7.641e-10 | 1.38e-05 |
CSE1L|1434 | 1.653e-09 | 2.99e-05 |
AMACR|23600 | 2.01e-09 | 3.63e-05 |
ZNF12|7559 | 2.13e-09 | 3.85e-05 |
RPIA|22934 | 2.177e-09 | 3.93e-05 |
NUFIP1|26747 | 2.249e-09 | 4.06e-05 |
Figure S3. Get High-res Image As an example, this figure shows the association of USPL1|10208 to 'HISTOLOGICAL.TYPE'. P value = 1.87e-10 with ANOVA analysis.

Table S9. Basic characteristics of clinical feature: 'PATHOLOGY.T'
PATHOLOGY.T | Mean (SD) | 2.88 (0.69) |
N | ||
T0 | 1 | |
T1 | 8 | |
T2 | 43 | |
T3 | 157 | |
T4 | 34 | |
Significant markers | N = 6 | |
pos. correlated | 6 | |
neg. correlated | 0 |
Table S10. Get Full Table List of 6 genes significantly correlated to 'PATHOLOGY.T' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
FSTL3|10272 | 0.3098 | 8.412e-07 | 0.0152 |
RAB6B|51560 | 0.3055 | 1.211e-06 | 0.0219 |
IGLON5|402665 | 0.3083 | 1.306e-06 | 0.0236 |
GADD45B|4616 | 0.3038 | 1.389e-06 | 0.0251 |
TRPC1|7220 | 0.3024 | 1.57e-06 | 0.0283 |
NGF|4803 | 0.3021 | 2.168e-06 | 0.0391 |
Figure S4. Get High-res Image As an example, this figure shows the association of FSTL3|10272 to 'PATHOLOGY.T'. P value = 8.41e-07 with Spearman correlation analysis.

Table S11. Basic characteristics of clinical feature: 'PATHOLOGY.N'
PATHOLOGY.N | Mean (SD) | 0.57 (0.76) |
N | ||
N0 | 143 | |
N1 | 57 | |
N2 | 40 | |
Significant markers | N = 41 | |
pos. correlated | 34 | |
neg. correlated | 7 |
Table S12. Get Full Table List of top 10 genes significantly correlated to 'PATHOLOGY.N' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
HAP1|9001 | 0.3462 | 6.191e-08 | 0.00112 |
MAP6|4135 | 0.3322 | 1.362e-07 | 0.00246 |
ATP5B|506 | -0.3244 | 2.766e-07 | 0.005 |
SUSD5|26032 | 0.3243 | 2.948e-07 | 0.00532 |
ZBTB20|26137 | 0.3232 | 3.075e-07 | 0.00555 |
CRLF1|9244 | 0.3315 | 3.186e-07 | 0.00575 |
CAMK2B|816 | 0.335 | 3.618e-07 | 0.00653 |
NACAD|23148 | 0.3208 | 3.809e-07 | 0.00688 |
LOC100126784|100126784 | 0.3205 | 4.615e-07 | 0.00833 |
GALNTL1|57452 | 0.3158 | 6.578e-07 | 0.0119 |
Figure S5. Get High-res Image As an example, this figure shows the association of HAP1|9001 to 'PATHOLOGY.N'. P value = 6.19e-08 with Spearman correlation analysis.

Table S13. Basic characteristics of clinical feature: 'PATHOLOGICSPREAD(M)'
PATHOLOGICSPREAD(M) | Labels | N |
M0 | 167 | |
M1 | 23 | |
M1A | 6 | |
M1B | 1 | |
MX | 42 | |
Significant markers | N = 12 |
Table S14. Get Full Table List of top 10 genes differentially expressed by 'PATHOLOGICSPREAD(M)'
ANOVA_P | Q | |
---|---|---|
TDRD12|91646 | 5.18e-11 | 9.36e-07 |
RB1|5925 | 3.032e-10 | 5.47e-06 |
FAM171A1|221061 | 1.454e-09 | 2.63e-05 |
ZNF558|148156 | 3.261e-09 | 5.89e-05 |
ZNF675|171392 | 5.534e-09 | 9.99e-05 |
TMEM185A|84548 | 1.037e-08 | 0.000187 |
ZNF845|91664 | 8.688e-08 | 0.00157 |
CCDC122|160857 | 1.751e-07 | 0.00316 |
ZNF28|7576 | 3.354e-07 | 0.00605 |
C13ORF31|144811 | 4.289e-07 | 0.00774 |
Figure S6. Get High-res Image As an example, this figure shows the association of TDRD12|91646 to 'PATHOLOGICSPREAD(M)'. P value = 5.18e-11 with ANOVA analysis.

Table S15. Basic characteristics of clinical feature: 'TUMOR.STAGE'
TUMOR.STAGE | Mean (SD) | 2.38 (0.94) |
N | ||
Stage 1 | 42 | |
Stage 2 | 91 | |
Stage 3 | 66 | |
Stage 4 | 32 | |
Significant markers | N = 18 | |
pos. correlated | 15 | |
neg. correlated | 3 |
Table S16. Get Full Table List of top 10 genes significantly correlated to 'TUMOR.STAGE' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
HAP1|9001 | 0.3707 | 1.13e-08 | 0.000204 |
CAMK2B|816 | 0.3676 | 3.753e-08 | 0.000678 |
TCHH|7062 | 0.3419 | 1.356e-07 | 0.00245 |
BEND3|57673 | -0.3358 | 1.704e-07 | 0.00308 |
UNG|7374 | -0.3334 | 2.112e-07 | 0.00381 |
TMEM59L|25789 | 0.3334 | 6.765e-07 | 0.0122 |
IGLON5|402665 | 0.3219 | 7.618e-07 | 0.0138 |
SHC1|6464 | 0.3165 | 9.084e-07 | 0.0164 |
PIM1|5292 | 0.3148 | 1.043e-06 | 0.0188 |
GADD45B|4616 | 0.3133 | 1.177e-06 | 0.0212 |
Figure S7. Get High-res Image As an example, this figure shows the association of HAP1|9001 to 'TUMOR.STAGE'. P value = 1.13e-08 with Spearman correlation analysis.

15 genes related to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
Table S17. Basic characteristics of clinical feature: 'RADIATIONS.RADIATION.REGIMENINDICATION'
RADIATIONS.RADIATION.REGIMENINDICATION | Labels | N |
NO | 5 | |
YES | 238 | |
Significant markers | N = 15 | |
Higher in YES | 4 | |
Higher in NO | 11 |
Table S18. Get Full Table List of top 10 genes differentially expressed by 'RADIATIONS.RADIATION.REGIMENINDICATION'
T(pos if higher in 'YES') | ttestP | Q | AUC | |
---|---|---|---|---|
ATP5L2|267020 | -8.68 | 7.927e-14 | 1.39e-09 | 0.7267 |
C1ORF74|148304 | -9.76 | 1.951e-10 | 3.43e-06 | 0.8067 |
GAPDHS|26330 | -11.7 | 3.979e-09 | 6.98e-05 | 0.8832 |
CDH22|64405 | 8.09 | 3.995e-08 | 0.000701 | 0.9074 |
KLHDC8A|55220 | -10.48 | 4.052e-08 | 0.000711 | 0.8471 |
SLC25A4|291 | 10.24 | 9.826e-08 | 0.00172 | 0.837 |
NCRNA00105|80161 | -7.45 | 1.863e-07 | 0.00327 | 0.7067 |
SLC22A11|55867 | -8.46 | 2.836e-07 | 0.00498 | 0.7325 |
NEUROD1|4760 | 7.8 | 4.017e-07 | 0.00705 | 0.8141 |
CLDN8|9073 | -10.55 | 5.517e-07 | 0.00968 | 0.8579 |
Figure S8. Get High-res Image As an example, this figure shows the association of ATP5L2|267020 to 'RADIATIONS.RADIATION.REGIMENINDICATION'. P value = 7.93e-14 with T-test analysis.

Table S19. Basic characteristics of clinical feature: 'COMPLETENESS.OF.RESECTION'
COMPLETENESS.OF.RESECTION | Labels | N |
R0 | 151 | |
R1 | 1 | |
R2 | 2 | |
RX | 25 | |
Significant markers | N = 2 |
Table S20. Get Full Table List of 2 genes differentially expressed by 'COMPLETENESS.OF.RESECTION'
ANOVA_P | Q | |
---|---|---|
ZNHIT2|741 | 1.714e-13 | 3.1e-09 |
OVCH1|341350 | 3.444e-09 | 6.22e-05 |
Figure S9. Get High-res Image As an example, this figure shows the association of ZNHIT2|741 to 'COMPLETENESS.OF.RESECTION'. P value = 1.71e-13 with ANOVA analysis.

Table S21. Basic characteristics of clinical feature: 'NUMBER.OF.LYMPH.NODES'
NUMBER.OF.LYMPH.NODES | Mean (SD) | 2.25 (5.2) |
Significant markers | N = 14 | |
pos. correlated | 12 | |
neg. correlated | 2 |
Table S22. Get Full Table List of top 10 genes significantly correlated to 'NUMBER.OF.LYMPH.NODES' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
NIPAL4|348938 | 0.3677 | 3.47e-08 | 0.000627 |
CRLF1|9244 | 0.3647 | 1.098e-07 | 0.00198 |
AMIGO2|347902 | 0.3403 | 3.81e-07 | 0.00688 |
LOC100126784|100126784 | 0.3363 | 6.002e-07 | 0.0108 |
C5ORF23|79614 | 0.334 | 6.819e-07 | 0.0123 |
PRSS35|167681 | 0.3333 | 7.238e-07 | 0.0131 |
SNCG|6623 | 0.33 | 8.914e-07 | 0.0161 |
TMEM59L|25789 | 0.3362 | 1.363e-06 | 0.0246 |
TCHH|7062 | 0.326 | 1.735e-06 | 0.0313 |
CAP2|10486 | 0.3214 | 1.765e-06 | 0.0319 |
Figure S10. Get High-res Image As an example, this figure shows the association of NIPAL4|348938 to 'NUMBER.OF.LYMPH.NODES'. P value = 3.47e-08 with Spearman correlation analysis. The straight line presents the best linear regression.

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Expresson data file = COADREAD-TP.uncv2.mRNAseq_RSEM_normalized_log2.txt
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Clinical data file = COADREAD-TP.clin.merged.picked.txt
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Number of patients = 243
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Number of genes = 18061
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Number of clinical features = 12
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.