GDAC_RNAseq_ConsensusClusteringPipeline Execution Log 2:39 PM Thu May 2, '13

Running as user: cgaadm_deadline

GDAC_TopgenesforCluster1 expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/SKCM-TM/3032890/SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_TopgenesforCluster1 selectedgenes 1500
GDAC_ConsensusClustering2 clustering algorithm HIERARCHICAL
GDAC_ConsensusClustering2 cluster by
GDAC_ConsensusClustering2 distance measure PEARSON
GDAC_ConsensusClustering2 normalize type -n2
GDAC_selectBestcluster3 measure Pearson
GDAC_selectBestcluster3 output SKCM-TM
GDAC_selectBestcluster3 inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/SKCM-TM/3032890/SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_CnmfReports4 report hclumRNAseq
Execution Times:
Submitted: 14:39:57 02-05-13
Completed:
Elapsed: 02 hrs 18 mins 19 secs

step 1. GDAC_TopgenesforCluster [id: 396325] This module can be used to selecte top genes with most variation, convert file to gct format, as well as imput missing value

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00205:29
expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/SKCM-TM/3032890/SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
selectedgenes 1500
outputprefix outputprefix
Output Files:
 .lsf.out
 outputprefix.expclu.gct
 stdout.txt
Execution Times:
Submitted: 14:39:58 02-05-13
Completed: 14:43:28 02-05-13
Elapsed: 00 hrs 03 mins 30 secs

step 2. GDAC_ConsensusClustering [id: 396326] Resampling-based clustering method

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00158:6
input filename outputprefix.expclu.gct
kmax 8
resampling iterations 20
seed value 12345
clustering algorithm HIERARCHICAL
cluster by
distance measure PEARSON
resample subsample
merge type average
descent iterations 2000
output stub <input.filename_basename>
normalize type -n2
normalization iterations 0
create heat map -p
heat map size 2
Output Files:
 outputprefix.expclu.sub192.stdev
 outputprefix.expclu.sub192.clsdist
 outputprefix.expclu.sub192.2.gct
 outputprefix.expclu.sub192.3.gct
 outputprefix.expclu.sub192.4.gct
 outputprefix.expclu.sub192.6.gct
 outputprefix.expclu.sub192.5.gct
 outputprefix.expclu.sub192.8.gct
 outputprefix.expclu.sub192.7.gct
 outputprefix.expclu.sub192.3.clu
 outputprefix.expclu.sub192.2.clu
 outputprefix.expclu.sub192.4.clu
 outputprefix.expclu.sub192.6.clu
 outputprefix.expclu.sub192.5.clu
 outputprefix.expclu.sub192.8.clu
 outputprefix.expclu.sub192.7.clu
 outputprefix.expclu.sub192.statistics.pdf
 outputprefix.expclu.sub192.srt.2.gct
 outputprefix.expclu.sub192.srt.2.gif
 outputprefix.expclu.sub192.srt.3.gif
 outputprefix.expclu.sub192.srt.3.gct
 outputprefix.expclu.sub192.srt.4.gif
 outputprefix.expclu.sub192.srt.4.gct
 outputprefix.expclu.sub192.srt.5.gct
 outputprefix.expclu.sub192.srt.5.gif
 outputprefix.expclu.sub192.srt.6.gct
 outputprefix.expclu.sub192.srt.6.gif
 outputprefix.expclu.sub192.srt.7.gct
 outputprefix.expclu.sub192.srt.7.gif
 outputprefix.expclu.sub192.srt.8.gct
 outputprefix.expclu.sub192.srt.8.gif
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 14:39:58 02-05-13
Completed: 16:47:23 02-05-13
Elapsed: 02 hrs 07 mins 25 secs

step 3. GDAC_selectBestcluster [id: 396327]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00207:32
measure Pearson
inputexp outputprefix.expclu.gct
output SKCM-TM
file clu 2 outputprefix.expclu.sub192.2.clu
file clu 3 outputprefix.expclu.sub192.3.clu
file clu 4 outputprefix.expclu.sub192.4.clu
file clu 5 outputprefix.expclu.sub192.5.clu
file clu 6 outputprefix.expclu.sub192.6.clu
file clu 7 outputprefix.expclu.sub192.7.clu
file clu 8 outputprefix.expclu.sub192.8.clu
inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/SKCM-TM/3032890/SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
Output Files:
 SKCM-TM.silfig.png
 SKCM-TM.bestclus.txt
 SKCM-TM.cormatrix.png
 SKCM-TM.allclusters.txt
 SKCM-TM.subclassmarkers.txt
 SKCM-TM.seclectedSubclassmarkers.txt
 SKCM-TM.geneheatmap.png
 SKCM-TM.geneheatmaptopgenes.png
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 14:39:58 02-05-13
Completed: 16:55:55 02-05-13
Elapsed: 02 hrs 15 mins 57 secs

step 4. GDAC_CnmfReports [id: 396328]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00414:21