This pipeline uses various statistical tests to identify miRs whose expression levels correlated to selected clinical features.
Testing the association between 540 genes and 9 clinical features across 40 samples, statistically thresholded by Q value < 0.05, 1 clinical feature related to at least one genes.
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5 genes correlated to 'HISTOLOGICAL.TYPE'.
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HSA-MIR-205 , HSA-MIR-192 , HSA-MIR-215 , HSA-MIR-194-2 , HSA-MIR-194-1
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No genes correlated to 'Time to Death', 'AGE', 'RADIATIONS.RADIATION.REGIMENINDICATION', 'NUMBERPACKYEARSSMOKED', 'TOBACCOSMOKINGHISTORYINDICATOR', 'DISTANT.METASTASIS', 'LYMPH.NODE.METASTASIS', and 'NUMBER.OF.LYMPH.NODES'.
Complete statistical result table is provided in Supplement Table 1
Table 1. Get Full Table This table shows the clinical features, statistical methods used, and the number of genes that are significantly associated with each clinical feature at Q value < 0.05.
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
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Time to Death | Cox regression test | N=0 | ||||
AGE | Spearman correlation test | N=0 | ||||
HISTOLOGICAL TYPE | ANOVA test | N=5 | ||||
RADIATIONS RADIATION REGIMENINDICATION | t test | N=0 | ||||
NUMBERPACKYEARSSMOKED | Spearman correlation test | N=0 | ||||
TOBACCOSMOKINGHISTORYINDICATOR | Spearman correlation test | N=0 | ||||
DISTANT METASTASIS | t test | N=0 | ||||
LYMPH NODE METASTASIS | t test | N=0 | ||||
NUMBER OF LYMPH NODES | Spearman correlation test | N=0 |
Table S1. Basic characteristics of clinical feature: 'Time to Death'
Time to Death | Duration (Months) | 0.1-177 (median=6.9) |
censored | N = 30 | |
death | N = 9 | |
Significant markers | N = 0 |
Table S2. Basic characteristics of clinical feature: 'AGE'
AGE | Mean (SD) | 49.02 (13) |
Significant markers | N = 0 |
Table S3. Basic characteristics of clinical feature: 'HISTOLOGICAL.TYPE'
HISTOLOGICAL.TYPE | Labels | N |
CERVICAL SQUAMOUS CELL CARCINOMA | 33 | |
ENDOCERVICAL TYPE OF ADENOCARCINOMA | 1 | |
SQUAMOUS CELL CARCINOMA | 6 | |
Significant markers | N = 5 |
Table S4. Get Full Table List of 5 genes differentially expressed by 'HISTOLOGICAL.TYPE'
ANOVA_P | Q | |
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HSA-MIR-205 | 1.303e-06 | 7e-04 |
HSA-MIR-192 | 2.348e-06 | 0.00126 |
HSA-MIR-215 | 6.992e-06 | 0.00374 |
HSA-MIR-194-2 | 1.901e-05 | 0.0102 |
HSA-MIR-194-1 | 2.547e-05 | 0.0136 |
Figure S1. Get High-res Image As an example, this figure shows the association of HSA-MIR-205 to 'HISTOLOGICAL.TYPE'. P value = 1.3e-06 with ANOVA analysis.
![](V3ex.png)
No gene related to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
Table S5. Basic characteristics of clinical feature: 'RADIATIONS.RADIATION.REGIMENINDICATION'
RADIATIONS.RADIATION.REGIMENINDICATION | Labels | N |
NO | 15 | |
YES | 25 | |
Significant markers | N = 0 |
Table S6. Basic characteristics of clinical feature: 'NUMBERPACKYEARSSMOKED'
NUMBERPACKYEARSSMOKED | Mean (SD) | 18.8 (11) |
Significant markers | N = 0 |
No gene related to 'TOBACCOSMOKINGHISTORYINDICATOR'.
Table S7. Basic characteristics of clinical feature: 'TOBACCOSMOKINGHISTORYINDICATOR'
TOBACCOSMOKINGHISTORYINDICATOR | Mean (SD) | 1.92 (1.1) |
Value | N | |
1 | 18 | |
2 | 11 | |
3 | 1 | |
4 | 7 | |
Significant markers | N = 0 |
Table S8. Basic characteristics of clinical feature: 'DISTANT.METASTASIS'
DISTANT.METASTASIS | Labels | N |
M0 | 26 | |
MX | 9 | |
Significant markers | N = 0 |
Table S9. Basic characteristics of clinical feature: 'LYMPH.NODE.METASTASIS'
LYMPH.NODE.METASTASIS | Labels | N |
N0 | 22 | |
N1 | 14 | |
Significant markers | N = 0 |
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Expresson data file = CESC-TP.miRseq_RPKM_log2.txt
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Clinical data file = CESC-TP.clin.merged.picked.txt
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Number of patients = 40
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Number of genes = 540
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Number of clinical features = 9
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.