This report serves to describe the mutational landscape and properties of a given individual set, as well as rank genes and genesets according to mutational significance. MutSigCV v0.9 was used to generate the results found in this report.
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Working with individual set: LAML-TB
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Number of patients in set: 197
The input for this pipeline is a set of individuals with the following files associated for each:
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An annotated .maf file describing the mutations called for the respective individual, and their properties.
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A .wig file that contains information about the coverage of the sample.
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MAF used for this analysis:LAML-TB.final_analysis_set.maf
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Significantly mutated genes (q ≤ 0.1): 0
The x axis represents the samples. The y axis represents the exons, one row per exon, and they are sorted by average coverage across samples. For exons with exactly the same average coverage, they are sorted next by the %GC of the exon. (The secondary sort is especially useful for the zero-coverage exons at the bottom).
Figure 1.
Figure 2. Patients counts and rates file used to generate this plot: LAML-TB.patients.counts_and_rates.txt
Column Descriptions:
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nnon = number of (nonsilent) mutations in this gene across the individual set
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npat = number of patients (individuals) with at least one nonsilent mutation
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nsite = number of unique sites having a non-silent mutation
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nflank = number of noncoding mutations from this gene's flanking region, across the individual set
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nsil = number of silent mutations in this gene across the individual set
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p = p-value (overall)
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q = q-value, False Discovery Rate (Benjamini-Hochberg procedure)
Table 1. Get Full Table A Ranked List of Significantly Mutated Genes. Number of significant genes found: 0. Number of genes displayed: 35. Click on a gene name to display its stick figure depicting the distribution of mutations and mutation types across the chosen gene (this feature may not be available for all significant genes).
| gene | Nnon | Nsil | Nflank | nnon | npat | nsite | nsil | nflank | nnei | fMLE | p | score | time | q |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A1BG | 157797 | 51417 | 217291 | 0 | 0 | 0 | 0 | 0 | 20 | 2.1 | 1 | 0 | NaN | 1 |
| A1CF | 297667 | 86680 | 470633 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | NaN | 1 |
| A2BP1 | 216109 | 59691 | 766527 | 0 | 0 | 0 | 0 | 0 | 10 | 2.1 | 1 | 0 | NaN | 1 |
| A2M | 614837 | 178285 | 956435 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| A2ML1 | 688515 | 197197 | 1490699 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | NaN | 1 |
| A4GALT | 133960 | 43537 | 15760 | 0 | 0 | 0 | 0 | 0 | 20 | 3.9 | 1 | 0 | NaN | 1 |
| A4GNT | 158585 | 43931 | 99091 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| AAAS | 306335 | 98303 | 602623 | 0 | 0 | 0 | 0 | 0 | 7 | 0.99 | 1 | 0 | NaN | 1 |
| AACS | 299440 | 83331 | 669209 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| AADAC | 186362 | 52796 | 176512 | 0 | 0 | 0 | 0 | 0 | 20 | 2.3 | 1 | 0 | NaN | 1 |
| AADACL2 | 188332 | 52008 | 176118 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| AADACL3 | 164298 | 46098 | 203698 | 0 | 0 | 0 | 0 | 0 | 20 | 1.5 | 1 | 0 | NaN | 1 |
| AADACL4 | 186953 | 55948 | 190302 | 0 | 0 | 0 | 0 | 0 | 20 | 1.5 | 1 | 0 | NaN | 1 |
| AADAT | 199758 | 52993 | 457040 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | NaN | 1 |
| AAGAB | 154054 | 40779 | 443053 | 0 | 0 | 0 | 0 | 0 | 19 | 2.9 | 1 | 0 | NaN | 1 |
| AAK1 | 339431 | 101061 | 697577 | 0 | 0 | 0 | 0 | 0 | 20 | 0.57 | 1 | 0 | NaN | 1 |
| AAMP | 252948 | 78209 | 488757 | 0 | 0 | 0 | 0 | 0 | 20 | 1.2 | 1 | 0 | NaN | 1 |
| AANAT | 63631 | 20291 | 75845 | 0 | 0 | 0 | 0 | 0 | 20 | 2.4 | 1 | 0 | NaN | 1 |
| AARS | 447978 | 129626 | 741311 | 0 | 0 | 0 | 0 | 0 | 20 | 0.75 | 1 | 0 | NaN | 1 |
| AARS2 | 428081 | 140658 | 791546 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| AARSD1 | 266935 | 74466 | 672164 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 1 | 0 | NaN | 1 |
| AASDH | 511609 | 143416 | 417443 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | NaN | 1 |
| AASDHPPT | 145583 | 38218 | 191287 | 0 | 0 | 0 | 0 | 0 | 11 | 0.9 | 1 | 0 | NaN | 1 |
| AASS | 442659 | 122731 | 895168 | 0 | 0 | 0 | 0 | 0 | 9 | 0.97 | 1 | 0 | NaN | 1 |
| AATF | 257282 | 67177 | 460980 | 0 | 0 | 0 | 0 | 0 | 20 | 0 | 1 | 0 | NaN | 1 |
| AATK | 181240 | 59297 | 97909 | 0 | 0 | 0 | 0 | 0 | 20 | 0.9 | 1 | 0 | NaN | 1 |
| ABAT | 236006 | 64419 | 608336 | 0 | 0 | 0 | 0 | 0 | 20 | 0.78 | 1 | 0 | NaN | 1 |
| ABCA1 | 1053162 | 297667 | 2047618 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | NaN | 1 |
| ABCA10 | 742099 | 193848 | 1385698 | 0 | 0 | 0 | 0 | 1 | 9 | 1.9 | 1 | 0 | NaN | 1 |
| ABCA12 | 1237160 | 341401 | 1967045 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | NaN | 1 |
| ABCA13 | 2177638 | 584499 | 1749360 | 0 | 0 | 0 | 0 | 0 | 19 | 0.77 | 1 | 0 | NaN | 1 |
| ABCA2 | 769876 | 232854 | 635522 | 0 | 0 | 0 | 0 | 0 | 20 | 1.1 | 1 | 0 | NaN | 1 |
| ABCA3 | 714716 | 218276 | 1140433 | 0 | 0 | 0 | 0 | 0 | 20 | 1.9 | 1 | 0 | NaN | 1 |
| ABCA4 | 1041342 | 300228 | 1884502 | 0 | 0 | 0 | 0 | 1 | 13 | 1.4 | 1 | 0 | NaN | 1 |
| ABCA5 | 774407 | 202713 | 1253117 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | NaN | 1 |
In brief, we tabulate the number of mutations and the number of covered bases for each gene. The counts are broken down by mutation context category: four context categories that are discovered by MutSig, and one for indel and 'null' mutations, which include indels, nonsense mutations, splice-site mutations, and non-stop (read-through) mutations. For each gene, we calculate the probability of seeing the observed constellation of mutations, i.e. the product P1 x P2 x ... x Pm, or a more extreme one, given the background mutation rates calculated across the dataset. [1]
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.