This pipeline uses various statistical tests to identify mRNAs whose expression levels correlated to selected clinical features.
Testing the association between 18084 genes and 12 clinical features across 271 samples, statistically thresholded by Q value < 0.05, 7 clinical features related to at least one genes.
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6 genes correlated to 'AGE'.
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ACOX2|8309 , FAM84B|157638 , MAOB|4129 , PRDX6|9588 , CMBL|134147 , ...
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219 genes correlated to 'PRIMARY.SITE.OF.DISEASE'.
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TMEM156|80008 , POU2AF1|5450 , IGJ|3512 , FCRL5|83416 , RBP5|83758 , ...
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1 gene correlated to 'NEOPLASM.DISEASESTAGE'.
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RAI14|26064
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2 genes correlated to 'PATHOLOGY.N.STAGE'.
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C12ORF62|84987 , ZNF131|7690
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1 gene correlated to 'PATHOLOGY.M.STAGE'.
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LRRC28|123355
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8 genes correlated to 'BRESLOW.THICKNESS'.
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GBP4|115361 , REEP6|92840 , TNFSF13B|10673 , GCNT1|2650 , SLC7A8|23428 , ...
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28 genes correlated to 'GENDER'.
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ZFY|7544 , PRKY|5616 , XIST|7503 , RPS4Y1|6192 , DDX3Y|8653 , ...
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No genes correlated to 'Time from Specimen Diagnosis to Death', 'Time to Death', 'PATHOLOGY.T.STAGE', 'MELANOMA.ULCERATION', and 'MELANOMA.PRIMARY.KNOWN'.
Complete statistical result table is provided in Supplement Table 1
Table 1. Get Full Table This table shows the clinical features, statistical methods used, and the number of genes that are significantly associated with each clinical feature at Q value < 0.05.
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
---|---|---|---|---|---|---|
Time from Specimen Diagnosis to Death | Cox regression test | N=0 | ||||
Time to Death | Cox regression test | N=0 | ||||
AGE | Spearman correlation test | N=6 | older | N=0 | younger | N=6 |
PRIMARY SITE OF DISEASE | ANOVA test | N=219 | ||||
NEOPLASM DISEASESTAGE | ANOVA test | N=1 | ||||
PATHOLOGY T STAGE | Spearman correlation test | N=0 | ||||
PATHOLOGY N STAGE | Spearman correlation test | N=2 | higher stage | N=1 | lower stage | N=1 |
PATHOLOGY M STAGE | ANOVA test | N=1 | ||||
MELANOMA ULCERATION | t test | N=0 | ||||
MELANOMA PRIMARY KNOWN | t test | N=0 | ||||
BRESLOW THICKNESS | Spearman correlation test | N=8 | higher breslow.thickness | N=4 | lower breslow.thickness | N=4 |
GENDER | t test | N=28 | male | N=15 | female | N=13 |
No gene related to 'Time from Specimen Diagnosis to Death'.
Table S1. Basic characteristics of clinical feature: 'Time from Specimen Diagnosis to Death'
Time from Specimen Diagnosis to Death | Duration (Months) | 0-124.3 (median=13.7) |
censored | N = 133 | |
death | N = 127 | |
Significant markers | N = 0 |
Table S2. Basic characteristics of clinical feature: 'Time to Death'
Time to Death | Duration (Months) | 0.2-357.4 (median=47.5) |
censored | N = 138 | |
death | N = 128 | |
Significant markers | N = 0 |
Table S3. Basic characteristics of clinical feature: 'AGE'
AGE | Mean (SD) | 55.56 (16) |
Significant markers | N = 6 | |
pos. correlated | 0 | |
neg. correlated | 6 |
Table S4. Get Full Table List of 6 genes significantly correlated to 'AGE' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
ACOX2|8309 | -0.3277 | 4.211e-08 | 0.000762 |
FAM84B|157638 | -0.2967 | 7.93e-07 | 0.0143 |
MAOB|4129 | -0.2961 | 8.402e-07 | 0.0152 |
PRDX6|9588 | -0.2867 | 1.909e-06 | 0.0345 |
CMBL|134147 | -0.2867 | 1.912e-06 | 0.0346 |
PPP1R1B|84152 | -0.2979 | 2.265e-06 | 0.041 |
Figure S1. Get High-res Image As an example, this figure shows the association of ACOX2|8309 to 'AGE'. P value = 4.21e-08 with Spearman correlation analysis. The straight line presents the best linear regression.
![](V3ex.png)
Table S5. Basic characteristics of clinical feature: 'PRIMARY.SITE.OF.DISEASE'
PRIMARY.SITE.OF.DISEASE | Labels | N |
DISTANT METASTASIS | 36 | |
PRIMARY TUMOR | 1 | |
REGIONAL CUTANEOUS OR SUBCUTANEOUS TISSUE | 59 | |
REGIONAL LYMPH NODE | 174 | |
Significant markers | N = 219 |
Table S6. Get Full Table List of top 10 genes differentially expressed by 'PRIMARY.SITE.OF.DISEASE'
ANOVA_P | Q | |
---|---|---|
TMEM156|80008 | 4.06e-12 | 7.34e-08 |
POU2AF1|5450 | 2.79e-11 | 5.05e-07 |
IGJ|3512 | 9.943e-11 | 1.8e-06 |
FCRL5|83416 | 1.604e-10 | 2.9e-06 |
RBP5|83758 | 1.929e-10 | 3.49e-06 |
CD38|952 | 2.51e-10 | 4.54e-06 |
SHISA3|152573 | 3.51e-10 | 6.35e-06 |
C7|730 | 4.982e-10 | 9.01e-06 |
MYO7B|4648 | 7.729e-10 | 1.4e-05 |
P2RY10|27334 | 9.975e-10 | 1.8e-05 |
Figure S2. Get High-res Image As an example, this figure shows the association of TMEM156|80008 to 'PRIMARY.SITE.OF.DISEASE'. P value = 4.06e-12 with ANOVA analysis.
![](V4ex.png)
Table S7. Basic characteristics of clinical feature: 'NEOPLASM.DISEASESTAGE'
NEOPLASM.DISEASESTAGE | Labels | N |
I OR II NOS | 10 | |
STAGE 0 | 6 | |
STAGE I | 22 | |
STAGE IA | 10 | |
STAGE IB | 25 | |
STAGE II | 17 | |
STAGE IIA | 10 | |
STAGE IIB | 14 | |
STAGE IIC | 10 | |
STAGE III | 30 | |
STAGE IIIA | 11 | |
STAGE IIIB | 23 | |
STAGE IIIC | 44 | |
STAGE IV | 12 | |
Significant markers | N = 1 |
Table S8. Get Full Table List of one gene differentially expressed by 'NEOPLASM.DISEASESTAGE'
ANOVA_P | Q | |
---|---|---|
RAI14|26064 | 1.444e-06 | 0.0261 |
Figure S3. Get High-res Image As an example, this figure shows the association of RAI14|26064 to 'NEOPLASM.DISEASESTAGE'. P value = 1.44e-06 with ANOVA analysis.
![](V5ex.png)
Table S9. Basic characteristics of clinical feature: 'PATHOLOGY.T.STAGE'
PATHOLOGY.T.STAGE | Mean (SD) | 2.4 (1.3) |
N | ||
0 | 22 | |
1 | 30 | |
2 | 59 | |
3 | 52 | |
4 | 54 | |
Significant markers | N = 0 |
Table S10. Basic characteristics of clinical feature: 'PATHOLOGY.N.STAGE'
PATHOLOGY.N.STAGE | Mean (SD) | 0.83 (1.1) |
N | ||
0 | 139 | |
1 | 48 | |
2 | 31 | |
3 | 33 | |
Significant markers | N = 2 | |
pos. correlated | 1 | |
neg. correlated | 1 |
Table S11. Get Full Table List of 2 genes significantly correlated to 'PATHOLOGY.N.STAGE' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
C12ORF62|84987 | 0.3096 | 5.624e-07 | 0.0102 |
ZNF131|7690 | -0.2982 | 1.505e-06 | 0.0272 |
Figure S4. Get High-res Image As an example, this figure shows the association of C12ORF62|84987 to 'PATHOLOGY.N.STAGE'. P value = 5.62e-07 with Spearman correlation analysis.
![](V7ex.png)
Table S12. Basic characteristics of clinical feature: 'PATHOLOGY.M.STAGE'
PATHOLOGY.M.STAGE | Labels | N |
M0 | 242 | |
M1 | 4 | |
M1A | 2 | |
M1B | 2 | |
M1C | 5 | |
Significant markers | N = 1 |
Table S13. Get Full Table List of one gene differentially expressed by 'PATHOLOGY.M.STAGE'
ANOVA_P | Q | |
---|---|---|
LRRC28|123355 | 4.002e-07 | 0.00724 |
Figure S5. Get High-res Image As an example, this figure shows the association of LRRC28|123355 to 'PATHOLOGY.M.STAGE'. P value = 4e-07 with ANOVA analysis.
![](V8ex.png)
Table S14. Basic characteristics of clinical feature: 'MELANOMA.ULCERATION'
MELANOMA.ULCERATION | Labels | N |
NO | 100 | |
YES | 68 | |
Significant markers | N = 0 |
Table S15. Basic characteristics of clinical feature: 'MELANOMA.PRIMARY.KNOWN'
MELANOMA.PRIMARY.KNOWN | Labels | N |
NO | 32 | |
YES | 239 | |
Significant markers | N = 0 |
Table S16. Basic characteristics of clinical feature: 'BRESLOW.THICKNESS'
BRESLOW.THICKNESS | Mean (SD) | 3.54 (5) |
Significant markers | N = 8 | |
pos. correlated | 4 | |
neg. correlated | 4 |
Table S17. Get Full Table List of 8 genes significantly correlated to 'BRESLOW.THICKNESS' by Spearman correlation test
SpearmanCorr | corrP | Q | |
---|---|---|---|
GBP4|115361 | -0.3589 | 2.084e-07 | 0.00377 |
REEP6|92840 | 0.3506 | 4.108e-07 | 0.00743 |
TNFSF13B|10673 | -0.3394 | 1.003e-06 | 0.0181 |
GCNT1|2650 | -0.3371 | 1.194e-06 | 0.0216 |
SLC7A8|23428 | 0.3335 | 1.578e-06 | 0.0285 |
CRTAP|10491 | 0.3326 | 1.687e-06 | 0.0305 |
FGL2|10875 | -0.3292 | 2.183e-06 | 0.0395 |
CDK15|65061 | 0.3566 | 2.404e-06 | 0.0435 |
Figure S6. Get High-res Image As an example, this figure shows the association of GBP4|115361 to 'BRESLOW.THICKNESS'. P value = 2.08e-07 with Spearman correlation analysis. The straight line presents the best linear regression.
![](V11ex.png)
Table S18. Basic characteristics of clinical feature: 'GENDER'
GENDER | Labels | N |
FEMALE | 103 | |
MALE | 168 | |
Significant markers | N = 28 | |
Higher in MALE | 15 | |
Higher in FEMALE | 13 |
Table S19. Get Full Table List of top 10 genes differentially expressed by 'GENDER'
T(pos if higher in 'MALE') | ttestP | Q | AUC | |
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ZFY|7544 | 42.7 | 1.671e-111 | 3.02e-107 | 0.9989 |
PRKY|5616 | 35.22 | 8.999e-90 | 1.63e-85 | 0.9976 |
XIST|7503 | -24.72 | 9.157e-66 | 1.66e-61 | 0.9626 |
RPS4Y1|6192 | 36.9 | 5.046e-65 | 9.12e-61 | 1 |
DDX3Y|8653 | 41.22 | 9.106e-64 | 1.65e-59 | 1 |
EIF1AY|9086 | 43.53 | 3.057e-58 | 5.52e-54 | 1 |
KDM5D|8284 | 39.06 | 4.096e-52 | 7.4e-48 | 1 |
UTY|7404 | 35.55 | 5.798e-47 | 1.05e-42 | 1 |
USP9Y|8287 | 33.66 | 6.306e-44 | 1.14e-39 | 0.9996 |
TSIX|9383 | -17.16 | 3.558e-41 | 6.43e-37 | 0.9534 |
Figure S7. Get High-res Image As an example, this figure shows the association of ZFY|7544 to 'GENDER'. P value = 1.67e-111 with T-test analysis.
![](V12ex.png)
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Expresson data file = SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
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Clinical data file = SKCM-TM.merged_data.txt
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Number of patients = 271
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Number of genes = 18084
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Number of clinical features = 12
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.