This pipeline performs Gene Set Enrichment Analysis (GSEA) using The Broad Institute GSEA tool with MSigDB - Class2: Canonical Pathways gene sets. For a given phenotype subtype, it shows what pathways are significantly enriched in each subtype by comparing gene expression profiles between subtypes. Here, the phenotype is mRNAseq_cNMF subtypes having more than three samples and the input expression file "SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt" is generated in the pipeline mRNAseq_Preprocess in the stddata run. This pipeline has the following features:
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For each subtype, calculates enrichment scores (ES) using signal to noise (S2N) that checks similarity between subtypes in expression level then calculates p values through permutation test.
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Lists pathways significantly enriched in each phenotype subtype and their enrichment scores (ES).
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Lists top 20 core genes enriched in each significant gene set and their enrichment scores (ES).
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Checks if the top core genes are up-regulated or down-regulated.
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Checks if the top core genes are high expressed or low expressed.
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Checks if the top core genes are significantly differently expressed genes.
Table 1. Get Full Table basic data info
basic data info |
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Number of Gene Sets: 708 |
Number of samples: 368 |
Original number of Gene Sets: 1320 |
Maximum gene set size: 933 |
Table 2. Get Full Table pheno data info
phenotype info |
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pheno.type: 1 - 4 :[ clus1 ] 117 |
pheno.type: 2 - 4 :[ clus2 ] 73 |
pheno.type: 3 - 4 :[ clus3 ] 105 |
pheno.type: 4 - 4 :[ clus4 ] 73 |
For the expression subtypes of 18059 genes in 369 samples, GSEA found enriched gene sets in each cluster using 368 gene sets in MSigDB canonical pathways. Top enriched gene sets are listed as below.
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clus1
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Top enriched gene sets are KEGG CITRATE CYCLE TCA CYCLE, KEGG PENTOSE PHOSPHATE PATHWAY, KEGG OXIDATIVE PHOSPHORYLATION, KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM, KEGG AMINOACYL TRNA BIOSYNTHESIS, KEGG BASE EXCISION REPAIR, KEGG LYSOSOME, KEGG PARKINSONS DISEASE, PID MTOR 4PATHWAY, REACTOME PYRUVATE METABOLISM AND CITRIC ACID TCA CYCLE
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And common core enriched genes are ACO2, CS, IDH3G, SDHA, SDHB, SDHC, ATP5B, ATP5G1, CYC1, DLST
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clus2
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Top enriched gene sets are KEGG GLYCOLYSIS GLUCONEOGENESIS, KEGG FATTY ACID METABOLISM, KEGG ALANINE ASPARTATE AND GLUTAMATE METABOLISM, KEGG GLYCINE SERINE AND THREONINE METABOLISM, KEGG VALINE LEUCINE AND ISOLEUCINE DEGRADATION, KEGG HISTIDINE METABOLISM, KEGG TYROSINE METABOLISM, KEGG TRYPTOPHAN METABOLISM, KEGG O GLYCAN BIOSYNTHESIS, KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM
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And common core enriched genes are PSMA8, PSMB10, PSMB8, PSMB9, PSME1, PSME2, B2M, HLA-A, APOBEC3G, CD247
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clus3
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Top enriched gene sets are KEGG TGF BETA SIGNALING PATHWAY, KEGG ECM RECEPTOR INTERACTION, BIOCARTA ERK PATHWAY, BIOCARTA MPR PATHWAY, WNT SIGNALING, ST INTEGRIN SIGNALING PATHWAY, PID INTEGRIN1 PATHWAY, PID EPHBFWDPATHWAY, PID RET PATHWAY, PID NCADHERINPATHWAY
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And common core enriched genes are FGFR1, ACAN, B3GNT3, B3GNT7, B4GALT6, CHST1, CHST2, CHST5, CHST6, FMOD
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clus4
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Top enriched gene sets are KEGG LYSINE DEGRADATION, KEGG RIBOSOME, KEGG BASAL TRANSCRIPTION FACTORS, KEGG SPLICEOSOME, PID ATM PATHWAY, PID RETINOIC ACID PATHWAY, PID BARD1PATHWAY, PID P53REGULATIONPATHWAY, REACTOME TRANSLATION, REACTOME FORMATION OF THE TERNARY COMPLEX AND SUBSEQUENTLY THE 43S COMPLEX
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And common core enriched genes are HSPA1B, LSM2, MAGOH, NCBP1, RBM8A, AAAS, EIF4E, HNRNPD, LSM5, LSM6
Table 3. Get Full Table This table shows top 10 pathways which are significantly enriched in cluster clus1. It displays only significant gene sets satisfying nom.p.val.threshold (-1), fwer.p.val.threshold (-1) , fdr.q.val.threshold (0.25) and the default table is sorted by Normalized Enrichment Score (NES). Further details on NES statistics, please visit The Broad GSEA website.
GeneSet(GS) | Size(#genes) | genes.ES.table | ES | NES | NOM.p.val | FDR.q.val | FWER.p.val | Tag.. | Gene.. | Signal | FDR..median. | glob.p.val |
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KEGG CITRATE CYCLE TCA CYCLE | 29 | genes.ES.table | 0.55 | 2 | 0.01 | 0.5 | 0.28 | 0.66 | 0.23 | 0.5 | 0 | 0.12 |
KEGG PENTOSE PHOSPHATE PATHWAY | 27 | genes.ES.table | 0.39 | 1.3 | 0.17 | 1 | 1 | 0.44 | 0.13 | 0.39 | 1 | 0.72 |
KEGG OXIDATIVE PHOSPHORYLATION | 114 | genes.ES.table | 0.52 | 1.9 | 0.025 | 0.56 | 0.49 | 0.61 | 0.25 | 0.46 | 0 | 0.15 |
KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM | 43 | genes.ES.table | 0.4 | 1.5 | 0.081 | 1 | 0.98 | 0.37 | 0.14 | 0.32 | 0.69 | 0.35 |
KEGG AMINOACYL TRNA BIOSYNTHESIS | 41 | genes.ES.table | 0.39 | 1.5 | 0.11 | 1 | 0.98 | 0.58 | 0.31 | 0.41 | 0.73 | 0.37 |
KEGG BASE EXCISION REPAIR | 33 | genes.ES.table | 0.36 | 1.4 | 0.16 | 1 | 1 | 0.42 | 0.16 | 0.36 | 1 | 0.6 |
KEGG LYSOSOME | 120 | genes.ES.table | 0.48 | 1.8 | 0.058 | 0.57 | 0.68 | 0.48 | 0.19 | 0.39 | 0.22 | 0.16 |
KEGG PARKINSONS DISEASE | 111 | genes.ES.table | 0.33 | 1.3 | 0.24 | 1 | 1 | 0.53 | 0.25 | 0.4 | 1 | 0.64 |
PID MTOR 4PATHWAY | 67 | genes.ES.table | 0.28 | 1.4 | 0.098 | 1 | 0.99 | 0.3 | 0.21 | 0.24 | 0.94 | 0.47 |
REACTOME PYRUVATE METABOLISM AND CITRIC ACID TCA CYCLE | 40 | genes.ES.table | 0.4 | 1.6 | 0.062 | 0.72 | 0.89 | 0.55 | 0.23 | 0.42 | 0.38 | 0.23 |
Table S1. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
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1 | OGDHL | OGDHL | OGDHL | 674 | 0.21 | 0.056 | YES |
2 | ACO2 | ACO2 | ACO2 | 1111 | 0.15 | 0.1 | YES |
3 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.15 | YES |
4 | IDH3G | IDH3G | IDH3G | 1742 | 0.12 | 0.18 | YES |
5 | FH | FH | FH | 1757 | 0.11 | 0.23 | YES |
6 | PC | PC | PC | 2166 | 0.099 | 0.25 | YES |
7 | OGDH | OGDH | OGDH | 2329 | 0.094 | 0.29 | YES |
8 | DLST | DLST | DLST | 2435 | 0.09 | 0.32 | YES |
9 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.35 | YES |
10 | MDH2 | MDH2 | MDH2 | 2641 | 0.084 | 0.39 | YES |
11 | IDH1 | IDH1 | IDH1 | 3071 | 0.072 | 0.4 | YES |
12 | CS | CS | CS | 3267 | 0.067 | 0.42 | YES |
13 | MDH1 | MDH1 | MDH1 | 3345 | 0.066 | 0.44 | YES |
14 | PCK2 | PCK2 | PCK2 | 3394 | 0.064 | 0.47 | YES |
15 | SUCLG1 | SUCLG1 | SUCLG1 | 3465 | 0.062 | 0.49 | YES |
16 | ACO1 | ACO1 | ACO1 | 3490 | 0.062 | 0.52 | YES |
17 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.54 | YES |
18 | IDH3B | IDH3B | IDH3B | 4021 | 0.05 | 0.54 | YES |
19 | PDHA1 | PDHA1 | PDHA1 | 4203 | 0.046 | 0.55 | YES |
20 | IDH3A | IDH3A | IDH3A | 4915 | 0.033 | 0.52 | NO |
21 | PDHB | PDHB | PDHB | 5822 | 0.015 | 0.48 | NO |
22 | ACLY | ACLY | ACLY | 5857 | 0.014 | 0.48 | NO |
23 | DLD | DLD | DLD | 6664 | -0.003 | 0.44 | NO |
24 | IDH2 | IDH2 | IDH2 | 6874 | -0.0073 | 0.43 | NO |
25 | SUCLG2 | SUCLG2 | SUCLG2 | 6973 | -0.0094 | 0.43 | NO |
26 | DLAT | DLAT | DLAT | 7701 | -0.025 | 0.4 | NO |
27 | SUCLA2 | SUCLA2 | SUCLA2 | 10898 | -0.1 | 0.27 | NO |
28 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.28 | NO |
29 | PCK1 | PCK1 | PCK1 | 12994 | -0.16 | 0.28 | NO |
Figure S1. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG CITRATE CYCLE TCA CYCLE.

Figure S2. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG CITRATE CYCLE TCA CYCLE, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S2. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | ATP6V0D2 | ATP6V0D2 | ATP6V0D2 | 102 | 0.42 | 0.041 | YES |
2 | ATP6V0A4 | ATP6V0A4 | ATP6V0A4 | 104 | 0.42 | 0.087 | YES |
3 | COX6A2 | COX6A2 | COX6A2 | 814 | 0.18 | 0.068 | YES |
4 | ATP12A | ATP12A | ATP12A | 850 | 0.18 | 0.086 | YES |
5 | ATP6V0A1 | ATP6V0A1 | ATP6V0A1 | 865 | 0.18 | 0.1 | YES |
6 | ATP6V0C | ATP6V0C | ATP6V0C | 1046 | 0.16 | 0.11 | YES |
7 | ATP6AP1 | ATP6AP1 | ATP6AP1 | 1052 | 0.16 | 0.13 | YES |
8 | NDUFS8 | NDUFS8 | NDUFS8 | 1261 | 0.14 | 0.14 | YES |
9 | UQCRC1 | UQCRC1 | UQCRC1 | 1307 | 0.14 | 0.15 | YES |
10 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.16 | YES |
11 | ATP6V1F | ATP6V1F | ATP6V1F | 1379 | 0.14 | 0.17 | YES |
12 | ATP6V0B | ATP6V0B | ATP6V0B | 1399 | 0.13 | 0.19 | YES |
13 | NDUFS7 | NDUFS7 | NDUFS7 | 1490 | 0.13 | 0.2 | YES |
14 | ATP6V0D1 | ATP6V0D1 | ATP6V0D1 | 1523 | 0.13 | 0.21 | YES |
15 | NDUFA8 | NDUFA8 | NDUFA8 | 1529 | 0.13 | 0.22 | YES |
16 | NDUFV1 | NDUFV1 | NDUFV1 | 1720 | 0.12 | 0.23 | YES |
17 | NDUFB7 | NDUFB7 | NDUFB7 | 1722 | 0.12 | 0.24 | YES |
18 | NDUFA7 | NDUFA7 | NDUFA7 | 1789 | 0.11 | 0.25 | YES |
19 | NDUFB1 | NDUFB1 | NDUFB1 | 1811 | 0.11 | 0.26 | YES |
20 | ATP5G1 | ATP5G1 | ATP5G1 | 1828 | 0.11 | 0.27 | YES |
21 | NDUFA3 | NDUFA3 | NDUFA3 | 1850 | 0.11 | 0.28 | YES |
22 | NDUFB4 | NDUFB4 | NDUFB4 | 1875 | 0.11 | 0.29 | YES |
23 | ATP6V1D | ATP6V1D | ATP6V1D | 1922 | 0.11 | 0.3 | YES |
24 | COX6A1 | COX6A1 | COX6A1 | 1945 | 0.11 | 0.31 | YES |
25 | ATP6V1B2 | ATP6V1B2 | ATP6V1B2 | 1959 | 0.11 | 0.32 | YES |
26 | COX5A | COX5A | COX5A | 1995 | 0.1 | 0.33 | YES |
27 | NDUFV3 | NDUFV3 | NDUFV3 | 2013 | 0.1 | 0.34 | YES |
28 | LHPP | LHPP | LHPP | 2019 | 0.1 | 0.36 | YES |
29 | NDUFS6 | NDUFS6 | NDUFS6 | 2093 | 0.1 | 0.36 | YES |
30 | UQCR10 | UQCR10 | UQCR10 | 2251 | 0.096 | 0.36 | YES |
31 | COX8A | COX8A | COX8A | 2326 | 0.094 | 0.37 | YES |
32 | ATP5B | ATP5B | ATP5B | 2422 | 0.091 | 0.38 | YES |
33 | NDUFS2 | NDUFS2 | NDUFS2 | 2593 | 0.086 | 0.38 | YES |
34 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.38 | YES |
35 | ATP6V1E1 | ATP6V1E1 | ATP6V1E1 | 2621 | 0.085 | 0.39 | YES |
36 | ATP5D | ATP5D | ATP5D | 2637 | 0.084 | 0.4 | YES |
37 | COX5B | COX5B | COX5B | 2731 | 0.081 | 0.41 | YES |
38 | ATP6V0E2 | ATP6V0E2 | ATP6V0E2 | 2733 | 0.081 | 0.42 | YES |
39 | NDUFB10 | NDUFB10 | NDUFB10 | 2762 | 0.08 | 0.42 | YES |
40 | NDUFC2 | NDUFC2 | NDUFC2 | 2804 | 0.079 | 0.43 | YES |
41 | ATP5H | ATP5H | ATP5H | 2819 | 0.079 | 0.44 | YES |
42 | COX6B1 | COX6B1 | COX6B1 | 2935 | 0.076 | 0.44 | YES |
43 | CYC1 | CYC1 | CYC1 | 2968 | 0.075 | 0.44 | YES |
44 | NDUFS3 | NDUFS3 | NDUFS3 | 3106 | 0.071 | 0.45 | YES |
45 | NDUFB8 | NDUFB8 | NDUFB8 | 3211 | 0.069 | 0.45 | YES |
46 | NDUFA9 | NDUFA9 | NDUFA9 | 3290 | 0.067 | 0.45 | YES |
47 | NDUFA11 | NDUFA11 | NDUFA11 | 3335 | 0.066 | 0.46 | YES |
48 | UQCRQ | UQCRQ | UQCRQ | 3446 | 0.063 | 0.46 | YES |
49 | NDUFA6 | NDUFA6 | NDUFA6 | 3480 | 0.062 | 0.46 | YES |
50 | ATP6V1E2 | ATP6V1E2 | ATP6V1E2 | 3584 | 0.06 | 0.46 | YES |
51 | ATP5G3 | ATP5G3 | ATP5G3 | 3591 | 0.06 | 0.47 | YES |
52 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.48 | YES |
53 | COX8C | COX8C | COX8C | 3607 | 0.059 | 0.48 | YES |
54 | UQCRFS1 | UQCRFS1 | UQCRFS1 | 3695 | 0.057 | 0.48 | YES |
55 | COX7A2 | COX7A2 | COX7A2 | 3748 | 0.056 | 0.49 | YES |
56 | ATP5I | ATP5I | ATP5I | 3760 | 0.056 | 0.49 | YES |
57 | ATP6V1H | ATP6V1H | ATP6V1H | 3808 | 0.054 | 0.5 | YES |
58 | COX15 | COX15 | COX15 | 3923 | 0.052 | 0.49 | YES |
59 | NDUFA1 | NDUFA1 | NDUFA1 | 3925 | 0.052 | 0.5 | YES |
60 | ATP5G2 | ATP5G2 | ATP5G2 | 3929 | 0.052 | 0.51 | YES |
61 | ATP6V1G1 | ATP6V1G1 | ATP6V1G1 | 4183 | 0.047 | 0.5 | YES |
62 | ATP6V1C1 | ATP6V1C1 | ATP6V1C1 | 4254 | 0.045 | 0.5 | YES |
63 | NDUFB2 | NDUFB2 | NDUFB2 | 4276 | 0.045 | 0.5 | YES |
64 | NDUFA2 | NDUFA2 | NDUFA2 | 4307 | 0.044 | 0.5 | YES |
65 | NDUFB9 | NDUFB9 | NDUFB9 | 4343 | 0.044 | 0.51 | YES |
66 | NDUFS5 | NDUFS5 | NDUFS5 | 4378 | 0.043 | 0.51 | YES |
67 | NDUFV2 | NDUFV2 | NDUFV2 | 4452 | 0.041 | 0.51 | YES |
68 | COX17 | COX17 | COX17 | 4461 | 0.041 | 0.52 | YES |
69 | COX7A2L | COX7A2L | COX7A2L | 4485 | 0.041 | 0.52 | YES |
70 | TCIRG1 | TCIRG1 | TCIRG1 | 4511 | 0.04 | 0.52 | YES |
71 | ATP5J2 | ATP5J2 | ATP5J2 | 4667 | 0.037 | 0.52 | NO |
72 | COX6C | COX6C | COX6C | 4732 | 0.036 | 0.52 | NO |
73 | ATP6V0E1 | ATP6V0E1 | ATP6V0E1 | 4890 | 0.033 | 0.51 | NO |
74 | NDUFA10 | NDUFA10 | NDUFA10 | 4996 | 0.031 | 0.51 | NO |
75 | NDUFS1 | NDUFS1 | NDUFS1 | 5060 | 0.03 | 0.51 | NO |
76 | NDUFAB1 | NDUFAB1 | NDUFAB1 | 5100 | 0.029 | 0.51 | NO |
77 | COX4I1 | COX4I1 | COX4I1 | 5112 | 0.029 | 0.51 | NO |
78 | ATP5J | ATP5J | ATP5J | 5145 | 0.028 | 0.52 | NO |
79 | NDUFC1 | NDUFC1 | NDUFC1 | 5206 | 0.027 | 0.52 | NO |
80 | COX10 | COX10 | COX10 | 5384 | 0.023 | 0.51 | NO |
81 | UQCR11 | UQCR11 | UQCR11 | 5446 | 0.022 | 0.51 | NO |
82 | UQCRHL | UQCRHL | UQCRHL | 5513 | 0.021 | 0.5 | NO |
83 | UQCRH | UQCRH | UQCRH | 5542 | 0.02 | 0.51 | NO |
84 | ATP5A1 | ATP5A1 | ATP5A1 | 5577 | 0.02 | 0.51 | NO |
85 | PPA2 | PPA2 | PPA2 | 5742 | 0.016 | 0.5 | NO |
86 | NDUFB3 | NDUFB3 | NDUFB3 | 5816 | 0.015 | 0.5 | NO |
87 | COX7C | COX7C | COX7C | 5877 | 0.014 | 0.49 | NO |
88 | ATP5C1 | ATP5C1 | ATP5C1 | 5917 | 0.013 | 0.49 | NO |
89 | ATP5O | ATP5O | ATP5O | 6259 | 0.006 | 0.48 | NO |
90 | NDUFB6 | NDUFB6 | NDUFB6 | 6402 | 0.0031 | 0.47 | NO |
91 | UQCRB | UQCRB | UQCRB | 6467 | 0.0016 | 0.46 | NO |
92 | ATP6V1A | ATP6V1A | ATP6V1A | 6636 | -0.0024 | 0.46 | NO |
93 | ATP5F1 | ATP5F1 | ATP5F1 | 6936 | -0.0086 | 0.44 | NO |
94 | ATP4A | ATP4A | ATP4A | 7071 | -0.011 | 0.43 | NO |
95 | ATP5E | ATP5E | ATP5E | 7097 | -0.012 | 0.43 | NO |
96 | UQCRC2 | UQCRC2 | UQCRC2 | 7256 | -0.016 | 0.43 | NO |
97 | NDUFB5 | NDUFB5 | NDUFB5 | 7397 | -0.018 | 0.42 | NO |
98 | ATP5L | ATP5L | ATP5L | 7423 | -0.019 | 0.42 | NO |
99 | NDUFA4 | NDUFA4 | NDUFA4 | 7472 | -0.02 | 0.42 | NO |
100 | COX7B | COX7B | COX7B | 7610 | -0.023 | 0.42 | NO |
101 | COX6B2 | COX6B2 | COX6B2 | 7895 | -0.029 | 0.4 | NO |
102 | COX4I2 | COX4I2 | COX4I2 | 8358 | -0.04 | 0.38 | NO |
103 | ATP6V0A2 | ATP6V0A2 | ATP6V0A2 | 8947 | -0.054 | 0.36 | NO |
104 | COX11 | COX11 | COX11 | 9430 | -0.065 | 0.34 | NO |
105 | NDUFS4 | NDUFS4 | NDUFS4 | 9608 | -0.07 | 0.33 | NO |
106 | NDUFA5 | NDUFA5 | NDUFA5 | 9729 | -0.073 | 0.34 | NO |
107 | ATP6V1C2 | ATP6V1C2 | ATP6V1C2 | 10258 | -0.085 | 0.32 | NO |
108 | NDUFA4L2 | NDUFA4L2 | NDUFA4L2 | 10957 | -0.1 | 0.29 | NO |
109 | PPA1 | PPA1 | PPA1 | 11161 | -0.11 | 0.29 | NO |
110 | ATP6V1G2 | ATP6V1G2 | ATP6V1G2 | 11468 | -0.12 | 0.28 | NO |
111 | ATP6V1B1 | ATP6V1B1 | ATP6V1B1 | 11492 | -0.12 | 0.3 | NO |
112 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.3 | NO |
113 | COX7A1 | COX7A1 | COX7A1 | 13044 | -0.16 | 0.24 | NO |
114 | ATP4B | ATP4B | ATP4B | 16794 | -0.34 | 0.07 | NO |
Figure S3. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG PENTOSE PHOSPHATE PATHWAY.

Figure S4. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG PENTOSE PHOSPHATE PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S3. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | PDK2 | PDK2 | PDK2 | 985 | 0.16 | -0.033 | YES |
2 | ACO2 | ACO2 | ACO2 | 1111 | 0.15 | -0.02 | YES |
3 | NDUFS8 | NDUFS8 | NDUFS8 | 1261 | 0.14 | -0.009 | YES |
4 | UQCRC1 | UQCRC1 | UQCRC1 | 1307 | 0.14 | 0.0071 | YES |
5 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.024 | YES |
6 | NDUFS7 | NDUFS7 | NDUFS7 | 1490 | 0.13 | 0.033 | YES |
7 | NDUFA8 | NDUFA8 | NDUFA8 | 1529 | 0.13 | 0.047 | YES |
8 | NDUFV1 | NDUFV1 | NDUFV1 | 1720 | 0.12 | 0.052 | YES |
9 | NDUFB7 | NDUFB7 | NDUFB7 | 1722 | 0.12 | 0.068 | YES |
10 | IDH3G | IDH3G | IDH3G | 1742 | 0.12 | 0.082 | YES |
11 | FH | FH | FH | 1757 | 0.11 | 0.096 | YES |
12 | NDUFA7 | NDUFA7 | NDUFA7 | 1789 | 0.11 | 0.11 | YES |
13 | NDUFB1 | NDUFB1 | NDUFB1 | 1811 | 0.11 | 0.12 | YES |
14 | ATP5G1 | ATP5G1 | ATP5G1 | 1828 | 0.11 | 0.14 | YES |
15 | NDUFA3 | NDUFA3 | NDUFA3 | 1850 | 0.11 | 0.15 | YES |
16 | NDUFB4 | NDUFB4 | NDUFB4 | 1875 | 0.11 | 0.16 | YES |
17 | COX6A1 | COX6A1 | COX6A1 | 1945 | 0.11 | 0.17 | YES |
18 | COX5A | COX5A | COX5A | 1995 | 0.1 | 0.18 | YES |
19 | NDUFV3 | NDUFV3 | NDUFV3 | 2013 | 0.1 | 0.2 | YES |
20 | NDUFS6 | NDUFS6 | NDUFS6 | 2093 | 0.1 | 0.21 | YES |
21 | COX8A | COX8A | COX8A | 2326 | 0.094 | 0.21 | YES |
22 | OGDH | OGDH | OGDH | 2329 | 0.094 | 0.22 | YES |
23 | ATP5B | ATP5B | ATP5B | 2422 | 0.091 | 0.23 | YES |
24 | DLST | DLST | DLST | 2435 | 0.09 | 0.24 | YES |
25 | SLC16A3 | SLC16A3 | SLC16A3 | 2517 | 0.088 | 0.24 | YES |
26 | NDUFS2 | NDUFS2 | NDUFS2 | 2593 | 0.086 | 0.25 | YES |
27 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.26 | YES |
28 | ATP5D | ATP5D | ATP5D | 2637 | 0.084 | 0.27 | YES |
29 | MDH2 | MDH2 | MDH2 | 2641 | 0.084 | 0.28 | YES |
30 | COX5B | COX5B | COX5B | 2731 | 0.081 | 0.29 | YES |
31 | NDUFB10 | NDUFB10 | NDUFB10 | 2762 | 0.08 | 0.3 | YES |
32 | NDUFC2 | NDUFC2 | NDUFC2 | 2804 | 0.079 | 0.3 | YES |
33 | BSG | BSG | BSG | 2813 | 0.079 | 0.32 | YES |
34 | ATP5H | ATP5H | ATP5H | 2819 | 0.079 | 0.33 | YES |
35 | PDK3 | PDK3 | PDK3 | 2865 | 0.078 | 0.33 | YES |
36 | NDUFA13 | NDUFA13 | NDUFA13 | 2879 | 0.077 | 0.34 | YES |
37 | COX6B1 | COX6B1 | COX6B1 | 2935 | 0.076 | 0.35 | YES |
38 | CYC1 | CYC1 | CYC1 | 2968 | 0.075 | 0.36 | YES |
39 | IDH1 | IDH1 | IDH1 | 3071 | 0.072 | 0.36 | YES |
40 | NDUFS3 | NDUFS3 | NDUFS3 | 3106 | 0.071 | 0.37 | YES |
41 | NDUFB8 | NDUFB8 | NDUFB8 | 3211 | 0.069 | 0.37 | YES |
42 | CS | CS | CS | 3267 | 0.067 | 0.38 | YES |
43 | NDUFA9 | NDUFA9 | NDUFA9 | 3290 | 0.067 | 0.39 | YES |
44 | NDUFA11 | NDUFA11 | NDUFA11 | 3335 | 0.066 | 0.39 | YES |
45 | UQCRQ | UQCRQ | UQCRQ | 3446 | 0.063 | 0.4 | YES |
46 | SUCLG1 | SUCLG1 | SUCLG1 | 3465 | 0.062 | 0.4 | YES |
47 | NDUFA6 | NDUFA6 | NDUFA6 | 3480 | 0.062 | 0.41 | YES |
48 | ADHFE1 | ADHFE1 | ADHFE1 | 3517 | 0.061 | 0.42 | YES |
49 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.42 | YES |
50 | UQCRFS1 | UQCRFS1 | UQCRFS1 | 3695 | 0.057 | 0.42 | YES |
51 | ATP5I | ATP5I | ATP5I | 3760 | 0.056 | 0.42 | YES |
52 | NDUFA1 | NDUFA1 | NDUFA1 | 3925 | 0.052 | 0.42 | YES |
53 | LDHA | LDHA | LDHA | 3992 | 0.05 | 0.43 | YES |
54 | IDH3B | IDH3B | IDH3B | 4021 | 0.05 | 0.43 | YES |
55 | PDP2 | PDP2 | PDP2 | 4039 | 0.05 | 0.44 | YES |
56 | PDP1 | PDP1 | PDP1 | 4088 | 0.048 | 0.44 | YES |
57 | PDHA1 | PDHA1 | PDHA1 | 4203 | 0.046 | 0.44 | YES |
58 | NDUFB2 | NDUFB2 | NDUFB2 | 4276 | 0.045 | 0.44 | YES |
59 | NDUFA2 | NDUFA2 | NDUFA2 | 4307 | 0.044 | 0.45 | YES |
60 | NDUFB9 | NDUFB9 | NDUFB9 | 4343 | 0.044 | 0.45 | YES |
61 | NDUFS5 | NDUFS5 | NDUFS5 | 4378 | 0.043 | 0.45 | YES |
62 | NDUFV2 | NDUFV2 | NDUFV2 | 4452 | 0.041 | 0.46 | YES |
63 | COX7A2L | COX7A2L | COX7A2L | 4485 | 0.041 | 0.46 | YES |
64 | ATP5J2 | ATP5J2 | ATP5J2 | 4667 | 0.037 | 0.45 | YES |
65 | COX6C | COX6C | COX6C | 4732 | 0.036 | 0.46 | YES |
66 | NNT | NNT | NNT | 4888 | 0.033 | 0.45 | YES |
67 | IDH3A | IDH3A | IDH3A | 4915 | 0.033 | 0.45 | YES |
68 | NDUFA10 | NDUFA10 | NDUFA10 | 4996 | 0.031 | 0.45 | YES |
69 | NDUFS1 | NDUFS1 | NDUFS1 | 5060 | 0.03 | 0.45 | YES |
70 | NDUFAB1 | NDUFAB1 | NDUFAB1 | 5100 | 0.029 | 0.46 | YES |
71 | COX4I1 | COX4I1 | COX4I1 | 5112 | 0.029 | 0.46 | YES |
72 | ATP5J | ATP5J | ATP5J | 5145 | 0.028 | 0.46 | YES |
73 | NDUFA12 | NDUFA12 | NDUFA12 | 5187 | 0.027 | 0.46 | YES |
74 | NDUFC1 | NDUFC1 | NDUFC1 | 5206 | 0.027 | 0.46 | YES |
75 | UQCR11 | UQCR11 | UQCR11 | 5446 | 0.022 | 0.45 | NO |
76 | UQCRHL | UQCRHL | UQCRHL | 5513 | 0.021 | 0.45 | NO |
77 | UQCRH | UQCRH | UQCRH | 5542 | 0.02 | 0.45 | NO |
78 | ATP5A1 | ATP5A1 | ATP5A1 | 5577 | 0.02 | 0.46 | NO |
79 | LDHB | LDHB | LDHB | 5683 | 0.017 | 0.45 | NO |
80 | NDUFB3 | NDUFB3 | NDUFB3 | 5816 | 0.015 | 0.45 | NO |
81 | PDHB | PDHB | PDHB | 5822 | 0.015 | 0.45 | NO |
82 | COX7C | COX7C | COX7C | 5877 | 0.014 | 0.45 | NO |
83 | ATP5C1 | ATP5C1 | ATP5C1 | 5917 | 0.013 | 0.45 | NO |
84 | L2HGDH | L2HGDH | L2HGDH | 5944 | 0.012 | 0.45 | NO |
85 | ETFB | ETFB | ETFB | 5979 | 0.011 | 0.45 | NO |
86 | CYCS | CYCS | CYCS | 6146 | 0.0083 | 0.44 | NO |
87 | PDPR | PDPR | PDPR | 6244 | 0.0063 | 0.43 | NO |
88 | ATP5O | ATP5O | ATP5O | 6259 | 0.006 | 0.43 | NO |
89 | NDUFB6 | NDUFB6 | NDUFB6 | 6402 | 0.0031 | 0.42 | NO |
90 | UQCRB | UQCRB | UQCRB | 6467 | 0.0016 | 0.42 | NO |
91 | DLD | DLD | DLD | 6664 | -0.003 | 0.41 | NO |
92 | ETFDH | ETFDH | ETFDH | 6748 | -0.0047 | 0.41 | NO |
93 | ETFA | ETFA | ETFA | 6757 | -0.0049 | 0.41 | NO |
94 | IDH2 | IDH2 | IDH2 | 6874 | -0.0073 | 0.4 | NO |
95 | ATP5F1 | ATP5F1 | ATP5F1 | 6936 | -0.0086 | 0.4 | NO |
96 | SUCLG2 | SUCLG2 | SUCLG2 | 6973 | -0.0094 | 0.4 | NO |
97 | ATP5E | ATP5E | ATP5E | 7097 | -0.012 | 0.39 | NO |
98 | SLC16A1 | SLC16A1 | SLC16A1 | 7246 | -0.015 | 0.39 | NO |
99 | UQCRC2 | UQCRC2 | UQCRC2 | 7256 | -0.016 | 0.39 | NO |
100 | NDUFB5 | NDUFB5 | NDUFB5 | 7397 | -0.018 | 0.38 | NO |
101 | ATP5L | ATP5L | ATP5L | 7423 | -0.019 | 0.38 | NO |
102 | NDUFA4 | NDUFA4 | NDUFA4 | 7472 | -0.02 | 0.38 | NO |
103 | COX7B | COX7B | COX7B | 7610 | -0.023 | 0.38 | NO |
104 | DLAT | DLAT | DLAT | 7701 | -0.025 | 0.38 | NO |
105 | D2HGDH | D2HGDH | D2HGDH | 7940 | -0.03 | 0.37 | NO |
106 | PDHX | PDHX | PDHX | 9166 | -0.059 | 0.31 | NO |
107 | NDUFS4 | NDUFS4 | NDUFS4 | 9608 | -0.07 | 0.29 | NO |
108 | NDUFA5 | NDUFA5 | NDUFA5 | 9729 | -0.073 | 0.3 | NO |
109 | SLC16A8 | SLC16A8 | SLC16A8 | 10385 | -0.089 | 0.27 | NO |
110 | SUCLA2 | SUCLA2 | SUCLA2 | 10898 | -0.1 | 0.26 | NO |
111 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.23 | NO |
112 | UCP2 | UCP2 | UCP2 | 12335 | -0.14 | 0.21 | NO |
113 | PDK1 | PDK1 | PDK1 | 14394 | -0.21 | 0.12 | NO |
114 | PDK4 | PDK4 | PDK4 | 15628 | -0.27 | 0.091 | NO |
115 | UCP3 | UCP3 | UCP3 | 16602 | -0.33 | 0.08 | NO |
Figure S5. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG OXIDATIVE PHOSPHORYLATION.

Figure S6. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG OXIDATIVE PHOSPHORYLATION, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S4. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | ARSG | ARSG | ARSG | 40 | 0.52 | 0.041 | YES |
2 | ATP6V0D2 | ATP6V0D2 | ATP6V0D2 | 102 | 0.42 | 0.072 | YES |
3 | ATP6V0A4 | ATP6V0A4 | ATP6V0A4 | 104 | 0.42 | 0.11 | YES |
4 | CTSL2 | CTSL2 | CTSL2 | 128 | 0.4 | 0.14 | YES |
5 | GBA | GBA | GBA | 560 | 0.22 | 0.13 | YES |
6 | CTSF | CTSF | CTSF | 733 | 0.19 | 0.14 | YES |
7 | NAGLU | NAGLU | NAGLU | 785 | 0.19 | 0.15 | YES |
8 | ATP6V0A1 | ATP6V0A1 | ATP6V0A1 | 865 | 0.18 | 0.16 | YES |
9 | PLA2G15 | PLA2G15 | PLA2G15 | 880 | 0.18 | 0.18 | YES |
10 | CTSD | CTSD | CTSD | 958 | 0.17 | 0.19 | YES |
11 | ATP6V0C | ATP6V0C | ATP6V0C | 1046 | 0.16 | 0.19 | YES |
12 | ATP6AP1 | ATP6AP1 | ATP6AP1 | 1052 | 0.16 | 0.21 | YES |
13 | CTNS | CTNS | CTNS | 1071 | 0.16 | 0.22 | YES |
14 | CTSH | CTSH | CTSH | 1088 | 0.16 | 0.23 | YES |
15 | PPT2 | PPT2 | PPT2 | 1147 | 0.15 | 0.24 | YES |
16 | GLA | GLA | GLA | 1152 | 0.15 | 0.25 | YES |
17 | NPC1 | NPC1 | NPC1 | 1163 | 0.15 | 0.26 | YES |
18 | MCOLN1 | MCOLN1 | MCOLN1 | 1199 | 0.15 | 0.28 | YES |
19 | SORT1 | SORT1 | SORT1 | 1216 | 0.15 | 0.29 | YES |
20 | GM2A | GM2A | GM2A | 1227 | 0.15 | 0.3 | YES |
21 | SMPD1 | SMPD1 | SMPD1 | 1252 | 0.14 | 0.31 | YES |
22 | AP3B2 | AP3B2 | AP3B2 | 1339 | 0.14 | 0.32 | YES |
23 | CTSK | CTSK | CTSK | 1366 | 0.14 | 0.32 | YES |
24 | ATP6V0B | ATP6V0B | ATP6V0B | 1399 | 0.13 | 0.33 | YES |
25 | ATP6V0D1 | ATP6V0D1 | ATP6V0D1 | 1523 | 0.13 | 0.34 | YES |
26 | CTSA | CTSA | CTSA | 1533 | 0.13 | 0.35 | YES |
27 | ASAH1 | ASAH1 | ASAH1 | 1536 | 0.13 | 0.36 | YES |
28 | HEXA | HEXA | HEXA | 1577 | 0.12 | 0.37 | YES |
29 | DNASE2B | DNASE2B | DNASE2B | 1690 | 0.12 | 0.37 | YES |
30 | CTSZ | CTSZ | CTSZ | 1836 | 0.11 | 0.37 | YES |
31 | NEU1 | NEU1 | NEU1 | 1843 | 0.11 | 0.38 | YES |
32 | NAGA | NAGA | NAGA | 1878 | 0.11 | 0.39 | YES |
33 | CD63 | CD63 | CD63 | 1934 | 0.11 | 0.39 | YES |
34 | GLB1 | GLB1 | GLB1 | 1953 | 0.11 | 0.4 | YES |
35 | AP1S3 | AP1S3 | AP1S3 | 2111 | 0.1 | 0.4 | YES |
36 | NAGPA | NAGPA | NAGPA | 2147 | 0.099 | 0.41 | YES |
37 | CLN3 | CLN3 | CLN3 | 2256 | 0.096 | 0.41 | YES |
38 | ARSA | ARSA | ARSA | 2314 | 0.094 | 0.41 | YES |
39 | ACP5 | ACP5 | ACP5 | 2322 | 0.094 | 0.42 | YES |
40 | GGA1 | GGA1 | GGA1 | 2330 | 0.094 | 0.43 | YES |
41 | ABCB9 | ABCB9 | ABCB9 | 2442 | 0.09 | 0.43 | YES |
42 | TPP1 | TPP1 | TPP1 | 2461 | 0.09 | 0.44 | YES |
43 | CTSB | CTSB | CTSB | 2531 | 0.087 | 0.44 | YES |
44 | AP1M1 | AP1M1 | AP1M1 | 2555 | 0.087 | 0.44 | YES |
45 | AP1M2 | AP1M2 | AP1M2 | 2693 | 0.082 | 0.44 | YES |
46 | CTSL1 | CTSL1 | CTSL1 | 2870 | 0.077 | 0.44 | YES |
47 | NPC2 | NPC2 | NPC2 | 2892 | 0.077 | 0.45 | YES |
48 | IGF2R | IGF2R | IGF2R | 2905 | 0.076 | 0.45 | YES |
49 | SGSH | SGSH | SGSH | 2922 | 0.076 | 0.46 | YES |
50 | PSAP | PSAP | PSAP | 3099 | 0.071 | 0.45 | YES |
51 | ENTPD4 | ENTPD4 | ENTPD4 | 3109 | 0.071 | 0.46 | YES |
52 | AP1S1 | AP1S1 | AP1S1 | 3115 | 0.071 | 0.46 | YES |
53 | M6PR | M6PR | M6PR | 3173 | 0.07 | 0.47 | YES |
54 | GNPTG | GNPTG | GNPTG | 3294 | 0.067 | 0.46 | YES |
55 | AP3D1 | AP3D1 | AP3D1 | 3300 | 0.067 | 0.47 | YES |
56 | DNASE2 | DNASE2 | DNASE2 | 3313 | 0.066 | 0.48 | YES |
57 | GGA3 | GGA3 | GGA3 | 3403 | 0.064 | 0.48 | YES |
58 | AP3S2 | AP3S2 | AP3S2 | 3437 | 0.063 | 0.48 | YES |
59 | CLTB | CLTB | CLTB | 3691 | 0.057 | 0.47 | NO |
60 | ATP6V1H | ATP6V1H | ATP6V1H | 3808 | 0.054 | 0.47 | NO |
61 | CD68 | CD68 | CD68 | 4074 | 0.049 | 0.46 | NO |
62 | AP1B1 | AP1B1 | AP1B1 | 4076 | 0.049 | 0.46 | NO |
63 | AGA | AGA | AGA | 4099 | 0.048 | 0.46 | NO |
64 | LAMP1 | LAMP1 | LAMP1 | 4132 | 0.048 | 0.47 | NO |
65 | SUMF1 | SUMF1 | SUMF1 | 4198 | 0.046 | 0.47 | NO |
66 | AP3M2 | AP3M2 | AP3M2 | 4233 | 0.046 | 0.47 | NO |
67 | HGSNAT | HGSNAT | HGSNAT | 4238 | 0.046 | 0.47 | NO |
68 | MAN2B1 | MAN2B1 | MAN2B1 | 4292 | 0.045 | 0.47 | NO |
69 | GGA2 | GGA2 | GGA2 | 4329 | 0.044 | 0.47 | NO |
70 | TCIRG1 | TCIRG1 | TCIRG1 | 4511 | 0.04 | 0.47 | NO |
71 | CLTCL1 | CLTCL1 | CLTCL1 | 4569 | 0.039 | 0.47 | NO |
72 | LAPTM4B | LAPTM4B | LAPTM4B | 4718 | 0.036 | 0.46 | NO |
73 | GALNS | GALNS | GALNS | 4739 | 0.036 | 0.46 | NO |
74 | GUSB | GUSB | GUSB | 4801 | 0.035 | 0.46 | NO |
75 | SLC11A1 | SLC11A1 | SLC11A1 | 4860 | 0.034 | 0.46 | NO |
76 | LAMP2 | LAMP2 | LAMP2 | 4900 | 0.033 | 0.46 | NO |
77 | SLC11A2 | SLC11A2 | SLC11A2 | 4981 | 0.031 | 0.46 | NO |
78 | GNPTAB | GNPTAB | GNPTAB | 5215 | 0.027 | 0.45 | NO |
79 | ABCA2 | ABCA2 | ABCA2 | 5236 | 0.026 | 0.45 | NO |
80 | AP4M1 | AP4M1 | AP4M1 | 5274 | 0.026 | 0.45 | NO |
81 | GNS | GNS | GNS | 5325 | 0.024 | 0.45 | NO |
82 | CTSG | CTSG | CTSG | 5536 | 0.02 | 0.44 | NO |
83 | HEXB | HEXB | HEXB | 5657 | 0.018 | 0.44 | NO |
84 | GAA | GAA | GAA | 5714 | 0.017 | 0.43 | NO |
85 | SLC17A5 | SLC17A5 | SLC17A5 | 5932 | 0.012 | 0.42 | NO |
86 | CLTC | CLTC | CLTC | 5959 | 0.012 | 0.42 | NO |
87 | AP3M1 | AP3M1 | AP3M1 | 6209 | 0.0071 | 0.41 | NO |
88 | FUCA1 | FUCA1 | FUCA1 | 6317 | 0.0049 | 0.4 | NO |
89 | LIPA | LIPA | LIPA | 6662 | -0.003 | 0.38 | NO |
90 | LAPTM4A | LAPTM4A | LAPTM4A | 6753 | -0.0049 | 0.38 | NO |
91 | CLTA | CLTA | CLTA | 6862 | -0.0071 | 0.38 | NO |
92 | ACP2 | ACP2 | ACP2 | 7101 | -0.012 | 0.36 | NO |
93 | PPT1 | PPT1 | PPT1 | 7469 | -0.02 | 0.34 | NO |
94 | IDUA | IDUA | IDUA | 7518 | -0.021 | 0.34 | NO |
95 | LGMN | LGMN | LGMN | 7606 | -0.023 | 0.34 | NO |
96 | ARSB | ARSB | ARSB | 7653 | -0.024 | 0.34 | NO |
97 | AP1G1 | AP1G1 | AP1G1 | 8193 | -0.036 | 0.31 | NO |
98 | SCARB2 | SCARB2 | SCARB2 | 8485 | -0.044 | 0.3 | NO |
99 | AP3S1 | AP3S1 | AP3S1 | 8534 | -0.045 | 0.3 | NO |
100 | AP4S1 | AP4S1 | AP4S1 | 8765 | -0.05 | 0.29 | NO |
101 | IDS | IDS | IDS | 8788 | -0.051 | 0.3 | NO |
102 | ATP6V0A2 | ATP6V0A2 | ATP6V0A2 | 8947 | -0.054 | 0.29 | NO |
103 | AP4B1 | AP4B1 | AP4B1 | 9349 | -0.063 | 0.27 | NO |
104 | AP3B1 | AP3B1 | AP3B1 | 9710 | -0.072 | 0.26 | NO |
105 | CLN5 | CLN5 | CLN5 | 9941 | -0.078 | 0.25 | NO |
106 | AP1S2 | AP1S2 | AP1S2 | 10340 | -0.088 | 0.24 | NO |
107 | MANBA | MANBA | MANBA | 10790 | -0.099 | 0.22 | NO |
108 | CD164 | CD164 | CD164 | 11334 | -0.11 | 0.2 | NO |
109 | CTSE | CTSE | CTSE | 11351 | -0.11 | 0.21 | NO |
110 | AP4E1 | AP4E1 | AP4E1 | 11574 | -0.12 | 0.21 | NO |
111 | NAPSA | NAPSA | NAPSA | 11670 | -0.12 | 0.21 | NO |
112 | HYAL1 | HYAL1 | HYAL1 | 12634 | -0.15 | 0.17 | NO |
113 | CTSO | CTSO | CTSO | 12734 | -0.15 | 0.18 | NO |
114 | GALC | GALC | GALC | 13474 | -0.18 | 0.15 | NO |
115 | MFSD8 | MFSD8 | MFSD8 | 13671 | -0.18 | 0.16 | NO |
116 | CTSC | CTSC | CTSC | 13829 | -0.19 | 0.16 | NO |
117 | CTSS | CTSS | CTSS | 14093 | -0.2 | 0.16 | NO |
118 | LAPTM5 | LAPTM5 | LAPTM5 | 14623 | -0.22 | 0.15 | NO |
119 | CTSW | CTSW | CTSW | 14776 | -0.23 | 0.16 | NO |
120 | LAMP3 | LAMP3 | LAMP3 | 14953 | -0.24 | 0.17 | NO |
Figure S7. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM.

Figure S8. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S5. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | SAMM50 | SAMM50 | SAMM50 | 588 | 0.22 | 0.03 | YES |
2 | TIMM50 | TIMM50 | TIMM50 | 889 | 0.17 | 0.063 | YES |
3 | SLC25A4 | SLC25A4 | SLC25A4 | 1022 | 0.16 | 0.1 | YES |
4 | LDHD | LDHD | LDHD | 1049 | 0.16 | 0.15 | YES |
5 | ACO2 | ACO2 | ACO2 | 1111 | 0.15 | 0.19 | YES |
6 | MTX1 | MTX1 | MTX1 | 1430 | 0.13 | 0.21 | YES |
7 | IDH3G | IDH3G | IDH3G | 1742 | 0.12 | 0.22 | YES |
8 | ATP5G1 | ATP5G1 | ATP5G1 | 1828 | 0.11 | 0.25 | YES |
9 | PMPCA | PMPCA | PMPCA | 2035 | 0.1 | 0.27 | YES |
10 | FXN | FXN | FXN | 2202 | 0.097 | 0.28 | YES |
11 | ATP5B | ATP5B | ATP5B | 2422 | 0.091 | 0.3 | YES |
12 | VDAC1 | VDAC1 | VDAC1 | 2447 | 0.09 | 0.32 | YES |
13 | SLC25A13 | SLC25A13 | SLC25A13 | 2502 | 0.088 | 0.34 | YES |
14 | TOMM40 | TOMM40 | TOMM40 | 2598 | 0.086 | 0.36 | YES |
15 | CYC1 | CYC1 | CYC1 | 2968 | 0.075 | 0.36 | YES |
16 | GRPEL1 | GRPEL1 | GRPEL1 | 2969 | 0.075 | 0.39 | YES |
17 | COQ2 | COQ2 | COQ2 | 3032 | 0.073 | 0.4 | YES |
18 | CS | CS | CS | 3267 | 0.067 | 0.41 | YES |
19 | TOMM7 | TOMM7 | TOMM7 | 3386 | 0.065 | 0.42 | YES |
20 | CHCHD4 | CHCHD4 | CHCHD4 | 3399 | 0.064 | 0.44 | YES |
21 | BCS1L | BCS1L | BCS1L | 3508 | 0.062 | 0.45 | YES |
22 | TIMM44 | TIMM44 | TIMM44 | 3670 | 0.058 | 0.46 | YES |
23 | TIMM10 | TIMM10 | TIMM10 | 3865 | 0.053 | 0.46 | YES |
24 | TIMM13 | TIMM13 | TIMM13 | 3890 | 0.053 | 0.48 | YES |
25 | SLC25A6 | SLC25A6 | SLC25A6 | 3951 | 0.051 | 0.49 | YES |
26 | TAZ | TAZ | TAZ | 4063 | 0.049 | 0.5 | YES |
27 | COX17 | COX17 | COX17 | 4461 | 0.041 | 0.48 | YES |
28 | TIMM17B | TIMM17B | TIMM17B | 4516 | 0.04 | 0.49 | YES |
29 | TIMM22 | TIMM22 | TIMM22 | 4698 | 0.036 | 0.49 | YES |
30 | HSPA9 | HSPA9 | HSPA9 | 4710 | 0.036 | 0.5 | YES |
31 | GFER | GFER | GFER | 5271 | 0.026 | 0.48 | NO |
32 | ATP5A1 | ATP5A1 | ATP5A1 | 5577 | 0.02 | 0.47 | NO |
33 | TOMM22 | TOMM22 | TOMM22 | 5757 | 0.016 | 0.46 | NO |
34 | TIMM17A | TIMM17A | TIMM17A | 6355 | 0.0042 | 0.43 | NO |
35 | TOMM5 | TOMM5 | TOMM5 | 6681 | -0.0034 | 0.41 | NO |
36 | TIMM8A | TIMM8A | TIMM8A | 6919 | -0.0083 | 0.4 | NO |
37 | FXC1 | FXC1 | FXC1 | 7000 | -0.01 | 0.4 | NO |
38 | HSPD1 | HSPD1 | HSPD1 | 7154 | -0.013 | 0.4 | NO |
39 | TIMM9 | TIMM9 | TIMM9 | 7235 | -0.015 | 0.4 | NO |
40 | C18orf55 | C18orf55 | C18orf55 | 7372 | -0.018 | 0.39 | NO |
41 | TOMM20 | TOMM20 | TOMM20 | 8162 | -0.035 | 0.36 | NO |
42 | MTX2 | MTX2 | MTX2 | 8335 | -0.04 | 0.36 | NO |
43 | TOMM70A | TOMM70A | TOMM70A | 8873 | -0.053 | 0.35 | NO |
44 | DNAJC19 | DNAJC19 | DNAJC19 | 9020 | -0.056 | 0.36 | NO |
45 | TIMM8B | TIMM8B | TIMM8B | 9377 | -0.064 | 0.35 | NO |
46 | PMPCB | PMPCB | PMPCB | 9450 | -0.066 | 0.37 | NO |
47 | SLC25A12 | SLC25A12 | SLC25A12 | 10539 | -0.093 | 0.33 | NO |
48 | HSCB | HSCB | HSCB | 11099 | -0.11 | 0.33 | NO |
49 | GRPEL2 | GRPEL2 | GRPEL2 | 13637 | -0.18 | 0.24 | NO |
Figure S9. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG AMINOACYL TRNA BIOSYNTHESIS.

Figure S10. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG AMINOACYL TRNA BIOSYNTHESIS, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S6. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | NDUFS8 | NDUFS8 | NDUFS8 | 1261 | 0.14 | -0.034 | YES |
2 | UQCRC1 | UQCRC1 | UQCRC1 | 1307 | 0.14 | -0.0018 | YES |
3 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.032 | YES |
4 | NDUFS7 | NDUFS7 | NDUFS7 | 1490 | 0.13 | 0.055 | YES |
5 | NDUFA8 | NDUFA8 | NDUFA8 | 1529 | 0.13 | 0.085 | YES |
6 | NDUFV1 | NDUFV1 | NDUFV1 | 1720 | 0.12 | 0.1 | YES |
7 | NDUFB7 | NDUFB7 | NDUFB7 | 1722 | 0.12 | 0.13 | YES |
8 | NDUFA7 | NDUFA7 | NDUFA7 | 1789 | 0.11 | 0.16 | YES |
9 | NDUFB1 | NDUFB1 | NDUFB1 | 1811 | 0.11 | 0.18 | YES |
10 | NDUFA3 | NDUFA3 | NDUFA3 | 1850 | 0.11 | 0.21 | YES |
11 | NDUFB4 | NDUFB4 | NDUFB4 | 1875 | 0.11 | 0.24 | YES |
12 | COX6A1 | COX6A1 | COX6A1 | 1945 | 0.11 | 0.26 | YES |
13 | COX5A | COX5A | COX5A | 1995 | 0.1 | 0.28 | YES |
14 | NDUFV3 | NDUFV3 | NDUFV3 | 2013 | 0.1 | 0.31 | YES |
15 | NDUFS6 | NDUFS6 | NDUFS6 | 2093 | 0.1 | 0.33 | YES |
16 | COX8A | COX8A | COX8A | 2326 | 0.094 | 0.34 | YES |
17 | NDUFS2 | NDUFS2 | NDUFS2 | 2593 | 0.086 | 0.34 | YES |
18 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.36 | YES |
19 | COX5B | COX5B | COX5B | 2731 | 0.081 | 0.38 | YES |
20 | NDUFB10 | NDUFB10 | NDUFB10 | 2762 | 0.08 | 0.4 | YES |
21 | NDUFC2 | NDUFC2 | NDUFC2 | 2804 | 0.079 | 0.41 | YES |
22 | NDUFA13 | NDUFA13 | NDUFA13 | 2879 | 0.077 | 0.43 | YES |
23 | COX6B1 | COX6B1 | COX6B1 | 2935 | 0.076 | 0.44 | YES |
24 | CYC1 | CYC1 | CYC1 | 2968 | 0.075 | 0.46 | YES |
25 | NDUFS3 | NDUFS3 | NDUFS3 | 3106 | 0.071 | 0.47 | YES |
26 | NDUFB8 | NDUFB8 | NDUFB8 | 3211 | 0.069 | 0.48 | YES |
27 | NDUFA9 | NDUFA9 | NDUFA9 | 3290 | 0.067 | 0.5 | YES |
28 | NDUFA11 | NDUFA11 | NDUFA11 | 3335 | 0.066 | 0.51 | YES |
29 | UQCRQ | UQCRQ | UQCRQ | 3446 | 0.063 | 0.52 | YES |
30 | NDUFA6 | NDUFA6 | NDUFA6 | 3480 | 0.062 | 0.53 | YES |
31 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.54 | YES |
32 | UQCRFS1 | UQCRFS1 | UQCRFS1 | 3695 | 0.057 | 0.55 | YES |
33 | NDUFA1 | NDUFA1 | NDUFA1 | 3925 | 0.052 | 0.55 | YES |
34 | NDUFB2 | NDUFB2 | NDUFB2 | 4276 | 0.045 | 0.54 | YES |
35 | NDUFA2 | NDUFA2 | NDUFA2 | 4307 | 0.044 | 0.55 | YES |
36 | NDUFB9 | NDUFB9 | NDUFB9 | 4343 | 0.044 | 0.56 | YES |
37 | NDUFS5 | NDUFS5 | NDUFS5 | 4378 | 0.043 | 0.57 | YES |
38 | NDUFV2 | NDUFV2 | NDUFV2 | 4452 | 0.041 | 0.58 | YES |
39 | COX7A2L | COX7A2L | COX7A2L | 4485 | 0.041 | 0.58 | YES |
40 | COX6C | COX6C | COX6C | 4732 | 0.036 | 0.58 | YES |
41 | NDUFA10 | NDUFA10 | NDUFA10 | 4996 | 0.031 | 0.57 | YES |
42 | NDUFS1 | NDUFS1 | NDUFS1 | 5060 | 0.03 | 0.58 | YES |
43 | NDUFAB1 | NDUFAB1 | NDUFAB1 | 5100 | 0.029 | 0.58 | YES |
44 | COX4I1 | COX4I1 | COX4I1 | 5112 | 0.029 | 0.59 | YES |
45 | NDUFA12 | NDUFA12 | NDUFA12 | 5187 | 0.027 | 0.59 | YES |
46 | NDUFC1 | NDUFC1 | NDUFC1 | 5206 | 0.027 | 0.6 | YES |
47 | UQCR11 | UQCR11 | UQCR11 | 5446 | 0.022 | 0.59 | NO |
48 | UQCRHL | UQCRHL | UQCRHL | 5513 | 0.021 | 0.59 | NO |
49 | UQCRH | UQCRH | UQCRH | 5542 | 0.02 | 0.59 | NO |
50 | NDUFB3 | NDUFB3 | NDUFB3 | 5816 | 0.015 | 0.58 | NO |
51 | COX7C | COX7C | COX7C | 5877 | 0.014 | 0.58 | NO |
52 | ETFB | ETFB | ETFB | 5979 | 0.011 | 0.58 | NO |
53 | CYCS | CYCS | CYCS | 6146 | 0.0083 | 0.57 | NO |
54 | NDUFB6 | NDUFB6 | NDUFB6 | 6402 | 0.0031 | 0.56 | NO |
55 | UQCRB | UQCRB | UQCRB | 6467 | 0.0016 | 0.56 | NO |
56 | ETFDH | ETFDH | ETFDH | 6748 | -0.0047 | 0.54 | NO |
57 | ETFA | ETFA | ETFA | 6757 | -0.0049 | 0.54 | NO |
58 | UQCRC2 | UQCRC2 | UQCRC2 | 7256 | -0.016 | 0.52 | NO |
59 | NDUFB5 | NDUFB5 | NDUFB5 | 7397 | -0.018 | 0.52 | NO |
60 | NDUFA4 | NDUFA4 | NDUFA4 | 7472 | -0.02 | 0.52 | NO |
61 | COX7B | COX7B | COX7B | 7610 | -0.023 | 0.51 | NO |
62 | NDUFS4 | NDUFS4 | NDUFS4 | 9608 | -0.07 | 0.42 | NO |
63 | NDUFA5 | NDUFA5 | NDUFA5 | 9729 | -0.073 | 0.43 | NO |
64 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.36 | NO |
Figure S11. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG BASE EXCISION REPAIR.

Figure S12. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG BASE EXCISION REPAIR, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S7. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | PDK2 | PDK2 | PDK2 | 985 | 0.16 | 0.002 | YES |
2 | ACO2 | ACO2 | ACO2 | 1111 | 0.15 | 0.048 | YES |
3 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.084 | YES |
4 | IDH3G | IDH3G | IDH3G | 1742 | 0.12 | 0.1 | YES |
5 | FH | FH | FH | 1757 | 0.11 | 0.14 | YES |
6 | OGDH | OGDH | OGDH | 2329 | 0.094 | 0.14 | YES |
7 | DLST | DLST | DLST | 2435 | 0.09 | 0.16 | YES |
8 | SLC16A3 | SLC16A3 | SLC16A3 | 2517 | 0.088 | 0.19 | YES |
9 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.22 | YES |
10 | MDH2 | MDH2 | MDH2 | 2641 | 0.084 | 0.24 | YES |
11 | BSG | BSG | BSG | 2813 | 0.079 | 0.26 | YES |
12 | PDK3 | PDK3 | PDK3 | 2865 | 0.078 | 0.28 | YES |
13 | IDH1 | IDH1 | IDH1 | 3071 | 0.072 | 0.3 | YES |
14 | CS | CS | CS | 3267 | 0.067 | 0.31 | YES |
15 | SUCLG1 | SUCLG1 | SUCLG1 | 3465 | 0.062 | 0.32 | YES |
16 | ADHFE1 | ADHFE1 | ADHFE1 | 3517 | 0.061 | 0.34 | YES |
17 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.35 | YES |
18 | LDHA | LDHA | LDHA | 3992 | 0.05 | 0.35 | YES |
19 | IDH3B | IDH3B | IDH3B | 4021 | 0.05 | 0.36 | YES |
20 | PDP2 | PDP2 | PDP2 | 4039 | 0.05 | 0.38 | YES |
21 | PDP1 | PDP1 | PDP1 | 4088 | 0.048 | 0.4 | YES |
22 | PDHA1 | PDHA1 | PDHA1 | 4203 | 0.046 | 0.4 | YES |
23 | NNT | NNT | NNT | 4888 | 0.033 | 0.38 | NO |
24 | IDH3A | IDH3A | IDH3A | 4915 | 0.033 | 0.39 | NO |
25 | LDHB | LDHB | LDHB | 5683 | 0.017 | 0.35 | NO |
26 | PDHB | PDHB | PDHB | 5822 | 0.015 | 0.35 | NO |
27 | L2HGDH | L2HGDH | L2HGDH | 5944 | 0.012 | 0.35 | NO |
28 | PDPR | PDPR | PDPR | 6244 | 0.0063 | 0.33 | NO |
29 | DLD | DLD | DLD | 6664 | -0.003 | 0.31 | NO |
30 | IDH2 | IDH2 | IDH2 | 6874 | -0.0073 | 0.3 | NO |
31 | SUCLG2 | SUCLG2 | SUCLG2 | 6973 | -0.0094 | 0.3 | NO |
32 | SLC16A1 | SLC16A1 | SLC16A1 | 7246 | -0.015 | 0.29 | NO |
33 | DLAT | DLAT | DLAT | 7701 | -0.025 | 0.27 | NO |
34 | D2HGDH | D2HGDH | D2HGDH | 7940 | -0.03 | 0.27 | NO |
35 | PDHX | PDHX | PDHX | 9166 | -0.059 | 0.22 | NO |
36 | SLC16A8 | SLC16A8 | SLC16A8 | 10385 | -0.089 | 0.18 | NO |
37 | SUCLA2 | SUCLA2 | SUCLA2 | 10898 | -0.1 | 0.19 | NO |
38 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.19 | NO |
39 | PDK1 | PDK1 | PDK1 | 14394 | -0.21 | 0.11 | NO |
40 | PDK4 | PDK4 | PDK4 | 15628 | -0.27 | 0.13 | NO |
Figure S13. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG LYSOSOME.

Figure S14. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG LYSOSOME, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S8. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | NDUFS8 | NDUFS8 | NDUFS8 | 1261 | 0.14 | -0.042 | YES |
2 | UQCRC1 | UQCRC1 | UQCRC1 | 1307 | 0.14 | -0.016 | YES |
3 | SDHA | SDHA | SDHA | 1330 | 0.14 | 0.01 | YES |
4 | NDUFS7 | NDUFS7 | NDUFS7 | 1490 | 0.13 | 0.027 | YES |
5 | NDUFA8 | NDUFA8 | NDUFA8 | 1529 | 0.13 | 0.05 | YES |
6 | NDUFV1 | NDUFV1 | NDUFV1 | 1720 | 0.12 | 0.062 | YES |
7 | NDUFB7 | NDUFB7 | NDUFB7 | 1722 | 0.12 | 0.086 | YES |
8 | NDUFA7 | NDUFA7 | NDUFA7 | 1789 | 0.11 | 0.1 | YES |
9 | NDUFB1 | NDUFB1 | NDUFB1 | 1811 | 0.11 | 0.12 | YES |
10 | ATP5G1 | ATP5G1 | ATP5G1 | 1828 | 0.11 | 0.15 | YES |
11 | NDUFA3 | NDUFA3 | NDUFA3 | 1850 | 0.11 | 0.17 | YES |
12 | NDUFB4 | NDUFB4 | NDUFB4 | 1875 | 0.11 | 0.19 | YES |
13 | COX6A1 | COX6A1 | COX6A1 | 1945 | 0.11 | 0.2 | YES |
14 | COX5A | COX5A | COX5A | 1995 | 0.1 | 0.22 | YES |
15 | NDUFV3 | NDUFV3 | NDUFV3 | 2013 | 0.1 | 0.24 | YES |
16 | NDUFS6 | NDUFS6 | NDUFS6 | 2093 | 0.1 | 0.26 | YES |
17 | COX8A | COX8A | COX8A | 2326 | 0.094 | 0.26 | YES |
18 | ATP5B | ATP5B | ATP5B | 2422 | 0.091 | 0.28 | YES |
19 | NDUFS2 | NDUFS2 | NDUFS2 | 2593 | 0.086 | 0.28 | YES |
20 | SDHB | SDHB | SDHB | 2604 | 0.085 | 0.3 | YES |
21 | ATP5D | ATP5D | ATP5D | 2637 | 0.084 | 0.32 | YES |
22 | COX5B | COX5B | COX5B | 2731 | 0.081 | 0.33 | YES |
23 | NDUFB10 | NDUFB10 | NDUFB10 | 2762 | 0.08 | 0.34 | YES |
24 | NDUFC2 | NDUFC2 | NDUFC2 | 2804 | 0.079 | 0.36 | YES |
25 | ATP5H | ATP5H | ATP5H | 2819 | 0.079 | 0.37 | YES |
26 | NDUFA13 | NDUFA13 | NDUFA13 | 2879 | 0.077 | 0.38 | YES |
27 | COX6B1 | COX6B1 | COX6B1 | 2935 | 0.076 | 0.39 | YES |
28 | CYC1 | CYC1 | CYC1 | 2968 | 0.075 | 0.41 | YES |
29 | NDUFS3 | NDUFS3 | NDUFS3 | 3106 | 0.071 | 0.41 | YES |
30 | NDUFB8 | NDUFB8 | NDUFB8 | 3211 | 0.069 | 0.42 | YES |
31 | NDUFA9 | NDUFA9 | NDUFA9 | 3290 | 0.067 | 0.43 | YES |
32 | NDUFA11 | NDUFA11 | NDUFA11 | 3335 | 0.066 | 0.44 | YES |
33 | UQCRQ | UQCRQ | UQCRQ | 3446 | 0.063 | 0.45 | YES |
34 | NDUFA6 | NDUFA6 | NDUFA6 | 3480 | 0.062 | 0.46 | YES |
35 | SDHC | SDHC | SDHC | 3604 | 0.059 | 0.46 | YES |
36 | UQCRFS1 | UQCRFS1 | UQCRFS1 | 3695 | 0.057 | 0.47 | YES |
37 | ATP5I | ATP5I | ATP5I | 3760 | 0.056 | 0.48 | YES |
38 | NDUFA1 | NDUFA1 | NDUFA1 | 3925 | 0.052 | 0.48 | YES |
39 | NDUFB2 | NDUFB2 | NDUFB2 | 4276 | 0.045 | 0.47 | YES |
40 | NDUFA2 | NDUFA2 | NDUFA2 | 4307 | 0.044 | 0.47 | YES |
41 | NDUFB9 | NDUFB9 | NDUFB9 | 4343 | 0.044 | 0.48 | YES |
42 | NDUFS5 | NDUFS5 | NDUFS5 | 4378 | 0.043 | 0.49 | YES |
43 | NDUFV2 | NDUFV2 | NDUFV2 | 4452 | 0.041 | 0.49 | YES |
44 | COX7A2L | COX7A2L | COX7A2L | 4485 | 0.041 | 0.5 | YES |
45 | ATP5J2 | ATP5J2 | ATP5J2 | 4667 | 0.037 | 0.5 | YES |
46 | COX6C | COX6C | COX6C | 4732 | 0.036 | 0.5 | YES |
47 | NDUFA10 | NDUFA10 | NDUFA10 | 4996 | 0.031 | 0.49 | YES |
48 | NDUFS1 | NDUFS1 | NDUFS1 | 5060 | 0.03 | 0.49 | YES |
49 | NDUFAB1 | NDUFAB1 | NDUFAB1 | 5100 | 0.029 | 0.5 | YES |
50 | COX4I1 | COX4I1 | COX4I1 | 5112 | 0.029 | 0.5 | YES |
51 | ATP5J | ATP5J | ATP5J | 5145 | 0.028 | 0.5 | YES |
52 | NDUFA12 | NDUFA12 | NDUFA12 | 5187 | 0.027 | 0.51 | YES |
53 | NDUFC1 | NDUFC1 | NDUFC1 | 5206 | 0.027 | 0.51 | YES |
54 | UQCR11 | UQCR11 | UQCR11 | 5446 | 0.022 | 0.5 | NO |
55 | UQCRHL | UQCRHL | UQCRHL | 5513 | 0.021 | 0.5 | NO |
56 | UQCRH | UQCRH | UQCRH | 5542 | 0.02 | 0.51 | NO |
57 | ATP5A1 | ATP5A1 | ATP5A1 | 5577 | 0.02 | 0.51 | NO |
58 | NDUFB3 | NDUFB3 | NDUFB3 | 5816 | 0.015 | 0.5 | NO |
59 | COX7C | COX7C | COX7C | 5877 | 0.014 | 0.5 | NO |
60 | ATP5C1 | ATP5C1 | ATP5C1 | 5917 | 0.013 | 0.5 | NO |
61 | ETFB | ETFB | ETFB | 5979 | 0.011 | 0.5 | NO |
62 | CYCS | CYCS | CYCS | 6146 | 0.0083 | 0.49 | NO |
63 | ATP5O | ATP5O | ATP5O | 6259 | 0.006 | 0.48 | NO |
64 | NDUFB6 | NDUFB6 | NDUFB6 | 6402 | 0.0031 | 0.48 | NO |
65 | UQCRB | UQCRB | UQCRB | 6467 | 0.0016 | 0.47 | NO |
66 | ETFDH | ETFDH | ETFDH | 6748 | -0.0047 | 0.46 | NO |
67 | ETFA | ETFA | ETFA | 6757 | -0.0049 | 0.46 | NO |
68 | ATP5F1 | ATP5F1 | ATP5F1 | 6936 | -0.0086 | 0.45 | NO |
69 | ATP5E | ATP5E | ATP5E | 7097 | -0.012 | 0.44 | NO |
70 | UQCRC2 | UQCRC2 | UQCRC2 | 7256 | -0.016 | 0.44 | NO |
71 | NDUFB5 | NDUFB5 | NDUFB5 | 7397 | -0.018 | 0.44 | NO |
72 | ATP5L | ATP5L | ATP5L | 7423 | -0.019 | 0.44 | NO |
73 | NDUFA4 | NDUFA4 | NDUFA4 | 7472 | -0.02 | 0.44 | NO |
74 | COX7B | COX7B | COX7B | 7610 | -0.023 | 0.44 | NO |
75 | NDUFS4 | NDUFS4 | NDUFS4 | 9608 | -0.07 | 0.34 | NO |
76 | NDUFA5 | NDUFA5 | NDUFA5 | 9729 | -0.073 | 0.35 | NO |
77 | SDHD | SDHD | SDHD | 11602 | -0.12 | 0.27 | NO |
78 | UCP2 | UCP2 | UCP2 | 12335 | -0.14 | 0.25 | NO |
79 | UCP3 | UCP3 | UCP3 | 16602 | -0.33 | 0.08 | NO |
Figure S15. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG PARKINSONS DISEASE.

Figure S16. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG PARKINSONS DISEASE, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S9. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | PARS2 | PARS2 | PARS2 | 1014 | 0.16 | 0.011 | YES |
2 | TARS2 | TARS2 | TARS2 | 1246 | 0.14 | 0.059 | YES |
3 | LARS2 | LARS2 | LARS2 | 1930 | 0.11 | 0.066 | YES |
4 | NARS2 | NARS2 | NARS2 | 1956 | 0.11 | 0.11 | YES |
5 | SARS2 | SARS2 | SARS2 | 2040 | 0.1 | 0.15 | YES |
6 | DARS2 | DARS2 | DARS2 | 2167 | 0.099 | 0.18 | YES |
7 | AARS2 | AARS2 | AARS2 | 2232 | 0.096 | 0.22 | YES |
8 | CARS2 | CARS2 | CARS2 | 2249 | 0.096 | 0.26 | YES |
9 | VARS2 | VARS2 | VARS2 | 2615 | 0.085 | 0.27 | YES |
10 | EARS2 | EARS2 | EARS2 | 2926 | 0.076 | 0.29 | YES |
11 | MARS2 | MARS2 | MARS2 | 2998 | 0.074 | 0.31 | YES |
12 | FARSA | FARSA | FARSA | 3414 | 0.064 | 0.32 | YES |
13 | VARS | VARS | VARS | 3572 | 0.06 | 0.33 | YES |
14 | QARS | QARS | QARS | 3660 | 0.058 | 0.35 | YES |
15 | YARS2 | YARS2 | YARS2 | 3751 | 0.056 | 0.37 | YES |
16 | AARS | AARS | AARS | 4097 | 0.048 | 0.37 | YES |
17 | MTFMT | MTFMT | MTFMT | 4380 | 0.043 | 0.37 | YES |
18 | SARS | SARS | SARS | 4529 | 0.04 | 0.38 | YES |
19 | NARS | NARS | NARS | 4929 | 0.032 | 0.37 | YES |
20 | WARS | WARS | WARS | 4999 | 0.031 | 0.38 | YES |
21 | WARS2 | WARS2 | WARS2 | 5308 | 0.025 | 0.38 | YES |
22 | IARS2 | IARS2 | IARS2 | 5432 | 0.022 | 0.38 | YES |
23 | MARS | MARS | MARS | 5519 | 0.021 | 0.38 | YES |
24 | HARS2 | HARS2 | HARS2 | 5547 | 0.02 | 0.39 | YES |
25 | YARS | YARS | YARS | 5763 | 0.016 | 0.38 | NO |
26 | FARS2 | FARS2 | FARS2 | 5798 | 0.015 | 0.39 | NO |
27 | GARS | GARS | GARS | 6055 | 0.01 | 0.38 | NO |
28 | HARS | HARS | HARS | 6307 | 0.0051 | 0.37 | NO |
29 | CARS | CARS | CARS | 6447 | 0.0022 | 0.36 | NO |
30 | FARSB | FARSB | FARSB | 6570 | -0.00061 | 0.35 | NO |
31 | IARS | IARS | IARS | 7309 | -0.017 | 0.32 | NO |
32 | TARSL2 | TARSL2 | TARSL2 | 7428 | -0.019 | 0.32 | NO |
33 | EPRS | EPRS | EPRS | 7544 | -0.022 | 0.32 | NO |
34 | RARS | RARS | RARS | 7703 | -0.025 | 0.32 | NO |
35 | KARS | KARS | KARS | 7767 | -0.027 | 0.33 | NO |
36 | TARS | TARS | TARS | 7840 | -0.028 | 0.34 | NO |
37 | LARS | LARS | LARS | 9005 | -0.056 | 0.3 | NO |
38 | PSTK | PSTK | PSTK | 9275 | -0.062 | 0.31 | NO |
39 | DARS | DARS | DARS | 10565 | -0.093 | 0.28 | NO |
40 | RARS2 | RARS2 | RARS2 | 11302 | -0.11 | 0.28 | NO |
41 | SEPSECS | SEPSECS | SEPSECS | 14559 | -0.22 | 0.19 | NO |
Figure S17. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID MTOR 4PATHWAY.

Figure S18. Get High-res Image For the top 5 core enriched genes in the pathway: PID MTOR 4PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit
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Table S10. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | MOCOS | MOCOS | MOCOS | 316 | 0.3 | 0.038 | YES |
2 | GSTO2 | GSTO2 | GSTO2 | 453 | 0.25 | 0.078 | YES |
3 | QPRT | QPRT | QPRT | 478 | 0.25 | 0.12 | YES |
4 | GSTO1 | GSTO1 | GSTO1 | 587 | 0.22 | 0.16 | YES |
5 | SLC19A2 | SLC19A2 | SLC19A2 | 894 | 0.17 | 0.17 | YES |
6 | SLC19A1 | SLC19A1 | SLC19A1 | 1344 | 0.14 | 0.17 | YES |
7 | CYB5R3 | CYB5R3 | CYB5R3 | 1453 | 0.13 | 0.19 | YES |
8 | SLC25A16 | SLC25A16 | SLC25A16 | 1681 | 0.12 | 0.2 | YES |
9 | SLC2A3 | SLC2A3 | SLC2A3 | 1777 | 0.11 | 0.22 | YES |
10 | THTPA | THTPA | THTPA | 1898 | 0.11 | 0.23 | YES |
11 | MOCS1 | MOCS1 | MOCS1 | 1970 | 0.11 | 0.25 | YES |
12 | FASN | FASN | FASN | 2044 | 0.1 | 0.26 | YES |
13 | NFS1 | NFS1 | NFS1 | 2190 | 0.098 | 0.27 | YES |
14 | FLAD1 | FLAD1 | FLAD1 | 2279 | 0.095 | 0.29 | YES |
15 | ACP5 | ACP5 | ACP5 | 2322 | 0.094 | 0.3 | YES |
16 | PANK4 | PANK4 | PANK4 | 2403 | 0.092 | 0.32 | YES |
17 | PNPO | PNPO | PNPO | 2663 | 0.084 | 0.32 | YES |
18 | COASY | COASY | COASY | 2730 | 0.081 | 0.33 | YES |
19 | NADSYN1 | NADSYN1 | NADSYN1 | 2831 | 0.078 | 0.34 | YES |
20 | SLC46A1 | SLC46A1 | SLC46A1 | 3124 | 0.071 | 0.33 | YES |
21 | MTHFD1 | MTHFD1 | MTHFD1 | 3171 | 0.07 | 0.34 | YES |
22 | SLC19A3 | SLC19A3 | SLC19A3 | 3269 | 0.067 | 0.35 | YES |
23 | MTHFR | MTHFR | MTHFR | 3443 | 0.063 | 0.35 | YES |
24 | PPCDC | PPCDC | PPCDC | 3472 | 0.062 | 0.36 | YES |
25 | SLC23A2 | SLC23A2 | SLC23A2 | 3557 | 0.06 | 0.37 | YES |
26 | SLC2A1 | SLC2A1 | SLC2A1 | 3884 | 0.053 | 0.36 | YES |
27 | ENPP1 | ENPP1 | ENPP1 | 4034 | 0.05 | 0.36 | YES |
28 | MOCS3 | MOCS3 | MOCS3 | 4331 | 0.044 | 0.36 | YES |
29 | FPGS | FPGS | FPGS | 4363 | 0.043 | 0.36 | YES |
30 | SLC5A6 | SLC5A6 | SLC5A6 | 4404 | 0.042 | 0.37 | YES |
31 | NADK | NADK | NADK | 4469 | 0.041 | 0.37 | YES |
32 | GPHN | GPHN | GPHN | 4510 | 0.04 | 0.38 | YES |
33 | PDXK | PDXK | PDXK | 4954 | 0.032 | 0.36 | NO |
34 | PANK1 | PANK1 | PANK1 | 5500 | 0.021 | 0.33 | NO |
35 | CYB5A | CYB5A | CYB5A | 6461 | 0.0018 | 0.28 | NO |
36 | PPCS | PPCS | PPCS | 6808 | -0.0061 | 0.26 | NO |
37 | PANK2 | PANK2 | PANK2 | 6925 | -0.0085 | 0.26 | NO |
38 | NMNAT1 | NMNAT1 | NMNAT1 | 7845 | -0.028 | 0.21 | NO |
39 | PANK3 | PANK3 | PANK3 | 8420 | -0.042 | 0.19 | NO |
40 | SHMT1 | SHMT1 | SHMT1 | 8728 | -0.049 | 0.18 | NO |
41 | SLC25A32 | SLC25A32 | SLC25A32 | 8857 | -0.052 | 0.18 | NO |
42 | NAMPT | NAMPT | NAMPT | 10252 | -0.085 | 0.12 | NO |
43 | DHFR | DHFR | DHFR | 10335 | -0.088 | 0.13 | NO |
44 | RFK | RFK | RFK | 11137 | -0.11 | 0.11 | NO |
45 | MOCS2 | MOCS2 | MOCS2 | 12402 | -0.14 | 0.064 | NO |
46 | AASDHPPT | AASDHPPT | AASDHPPT | 12475 | -0.14 | 0.087 | NO |
47 | NMNAT3 | NMNAT3 | NMNAT3 | 13629 | -0.18 | 0.057 | NO |
48 | TPK1 | TPK1 | TPK1 | 15365 | -0.25 | 0.0081 | NO |
49 | NMNAT2 | NMNAT2 | NMNAT2 | 16525 | -0.32 | 0.004 | NO |
50 | SLC23A1 | SLC23A1 | SLC23A1 | 17538 | -0.43 | 0.028 | NO |
Figure S19. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: REACTOME PYRUVATE METABOLISM AND CITRIC ACID TCA CYCLE.

Figure S20. Get High-res Image For the top 5 core enriched genes in the pathway: REACTOME PYRUVATE METABOLISM AND CITRIC ACID TCA CYCLE, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

For the top enriched genes, if you want to check whether they are
-
up-regulated, please check the list of up-regulated genes
-
down-regulated, please check the list of down-regulated genes
For the top enriched genes, if you want to check whether they are
-
highly expressed genes, please check the list of high (top 30%) expressed genes
-
low expressed genes, please check the list of low (bottom 30%) expressed genes
An expression pattern of top(30%)/middle(30%)/low(30%) in this subtype against other subtypes is available in a heatmap
For the top enriched genes, if you want to check whether they are
-
significantly differently expressed genes by eBayes lm fit, please check the list of significant genes
Table 4. Get Full Table This table shows top 10 pathways which are significantly enriched in cluster clus2. It displays only significant gene sets satisfying nom.p.val.threshold (-1), fwer.p.val.threshold (-1) , fdr.q.val.threshold (0.25) and the default table is sorted by Normalized Enrichment Score (NES). Further details on NES statistics, please visit The Broad GSEA website.
GeneSet(GS) | Size(#genes) | genes.ES.table | ES | NES | NOM.p.val | FDR.q.val | FWER.p.val | Tag.. | Gene.. | Signal | FDR..median. | glob.p.val |
---|---|---|---|---|---|---|---|---|---|---|---|---|
KEGG GLYCOLYSIS GLUCONEOGENESIS | 57 | genes.ES.table | 0.4 | 1.3 | 0.11 | 0.24 | 1 | 0.088 | 0.075 | 0.081 | 0.2 | 0 |
KEGG FATTY ACID METABOLISM | 37 | genes.ES.table | 0.49 | 1.6 | 0.041 | 0.1 | 0.96 | 0.32 | 0.28 | 0.24 | 0.065 | 0.001 |
KEGG ALANINE ASPARTATE AND GLUTAMATE METABOLISM | 30 | genes.ES.table | 0.44 | 1.3 | 0.14 | 0.24 | 1 | 0.17 | 0.13 | 0.15 | 0.21 | 0 |
KEGG GLYCINE SERINE AND THREONINE METABOLISM | 30 | genes.ES.table | 0.52 | 1.4 | 0.082 | 0.21 | 1 | 0.33 | 0.14 | 0.29 | 0.18 | 0 |
KEGG VALINE LEUCINE AND ISOLEUCINE DEGRADATION | 43 | genes.ES.table | 0.52 | 1.8 | 0.0082 | 0.048 | 0.52 | 0.12 | 0.11 | 0.1 | 0.01 | 0.004 |
KEGG HISTIDINE METABOLISM | 29 | genes.ES.table | 0.57 | 1.5 | 0.026 | 0.12 | 0.98 | 0.38 | 0.26 | 0.28 | 0.082 | 0 |
KEGG TYROSINE METABOLISM | 40 | genes.ES.table | 0.55 | 1.6 | 0.018 | 0.098 | 0.96 | 0.28 | 0.18 | 0.23 | 0.058 | 0.001 |
KEGG TRYPTOPHAN METABOLISM | 38 | genes.ES.table | 0.7 | 1.9 | 0 | 0.043 | 0.38 | 0.34 | 0.08 | 0.32 | 0 | 0.004 |
KEGG O GLYCAN BIOSYNTHESIS | 28 | genes.ES.table | 0.48 | 1.3 | 0.12 | 0.25 | 1 | 0.14 | 0.076 | 0.13 | 0.21 | 0 |
KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM | 43 | genes.ES.table | 0.34 | 1.3 | 0.17 | 0.24 | 1 | 0.07 | 0.1 | 0.063 | 0.21 | 0 |
Table S11. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CD247 | CD247 | CD247 | 104 | 0.87 | 0.069 | YES |
2 | CD8B | CD8B | CD8B | 129 | 0.85 | 0.14 | YES |
3 | LCK | LCK | LCK | 143 | 0.84 | 0.21 | YES |
4 | CD28 | CD28 | CD28 | 232 | 0.78 | 0.27 | YES |
5 | DOCK2 | DOCK2 | DOCK2 | 431 | 0.66 | 0.32 | YES |
6 | HCK | HCK | HCK | 648 | 0.56 | 0.36 | YES |
7 | APOBEC3G | APOBEC3G | APOBEC3G | 850 | 0.49 | 0.39 | YES |
8 | ELMO1 | ELMO1 | ELMO1 | 901 | 0.47 | 0.43 | YES |
9 | CD4 | CD4 | CD4 | 926 | 0.47 | 0.46 | YES |
10 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.5 | YES |
11 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.5 | YES |
12 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.51 | YES |
13 | NUP210 | NUP210 | NUP210 | 1833 | 0.27 | 0.53 | YES |
14 | B2M | B2M | B2M | 2091 | 0.24 | 0.53 | YES |
15 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.55 | YES |
16 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.56 | YES |
17 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.55 | YES |
18 | HLA-A | HLA-A | HLA-A | 2779 | 0.17 | 0.56 | YES |
19 | FYN | FYN | FYN | 3958 | 0.1 | 0.51 | NO |
20 | PSIP1 | PSIP1 | PSIP1 | 4484 | 0.085 | 0.48 | NO |
21 | PACS1 | PACS1 | PACS1 | 4869 | 0.073 | 0.47 | NO |
22 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.46 | NO |
23 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.44 | NO |
24 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.42 | NO |
25 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.41 | NO |
26 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.4 | NO |
27 | AP1B1 | AP1B1 | AP1B1 | 6502 | 0.038 | 0.4 | NO |
28 | CUL5 | CUL5 | CUL5 | 6669 | 0.035 | 0.4 | NO |
29 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.4 | NO |
30 | NUP54 | NUP54 | NUP54 | 6877 | 0.032 | 0.39 | NO |
31 | AP1S2 | AP1S2 | AP1S2 | 6890 | 0.031 | 0.39 | NO |
32 | NUPL2 | NUPL2 | NUPL2 | 6895 | 0.031 | 0.4 | NO |
33 | PAK2 | PAK2 | PAK2 | 6974 | 0.03 | 0.39 | NO |
34 | NUP43 | NUP43 | NUP43 | 7183 | 0.027 | 0.38 | NO |
35 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.38 | NO |
36 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.37 | NO |
37 | CDK9 | CDK9 | CDK9 | 7538 | 0.022 | 0.37 | NO |
38 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.37 | NO |
39 | RANBP2 | RANBP2 | RANBP2 | 7645 | 0.02 | 0.37 | NO |
40 | RBX1 | RBX1 | RBX1 | 7930 | 0.017 | 0.35 | NO |
41 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.35 | NO |
42 | NUP37 | NUP37 | NUP37 | 8141 | 0.014 | 0.34 | NO |
43 | AP1G1 | AP1G1 | AP1G1 | 8253 | 0.013 | 0.34 | NO |
44 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.33 | NO |
45 | NUP62 | NUP62 | NUP62 | 8448 | 0.0099 | 0.33 | NO |
46 | NUP88 | NUP88 | NUP88 | 8494 | 0.0093 | 0.33 | NO |
47 | NUP50 | NUP50 | NUP50 | 8627 | 0.0078 | 0.32 | NO |
48 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.31 | NO |
49 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.31 | NO |
50 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.3 | NO |
51 | TPR | TPR | TPR | 9011 | 0.0029 | 0.3 | NO |
52 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.3 | NO |
53 | XPO1 | XPO1 | XPO1 | 9118 | 0.0016 | 0.3 | NO |
54 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.29 | NO |
55 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.29 | NO |
56 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.28 | NO |
57 | NUP107 | NUP107 | NUP107 | 9601 | -0.0038 | 0.27 | NO |
58 | NUP85 | NUP85 | NUP85 | 9681 | -0.0047 | 0.27 | NO |
59 | ATP6V1H | ATP6V1H | ATP6V1H | 9799 | -0.0057 | 0.26 | NO |
60 | NUP153 | NUP153 | NUP153 | 10004 | -0.0081 | 0.25 | NO |
61 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.25 | NO |
62 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.25 | NO |
63 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.25 | NO |
64 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.25 | NO |
65 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.24 | NO |
66 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.24 | NO |
67 | KPNA1 | KPNA1 | KPNA1 | 10399 | -0.012 | 0.24 | NO |
68 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.23 | NO |
69 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.22 | NO |
70 | KPNB1 | KPNB1 | KPNB1 | 10689 | -0.015 | 0.22 | NO |
71 | AP2A2 | AP2A2 | AP2A2 | 10743 | -0.016 | 0.22 | NO |
72 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.22 | NO |
73 | AP2B1 | AP2B1 | AP2B1 | 10766 | -0.016 | 0.22 | NO |
74 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.22 | NO |
75 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.22 | NO |
76 | SLC25A4 | SLC25A4 | SLC25A4 | 11033 | -0.019 | 0.21 | NO |
77 | SLC25A5 | SLC25A5 | SLC25A5 | 11136 | -0.02 | 0.21 | NO |
78 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.21 | NO |
79 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.21 | NO |
80 | AP2M1 | AP2M1 | AP2M1 | 11260 | -0.021 | 0.21 | NO |
81 | NUP205 | NUP205 | NUP205 | 11382 | -0.022 | 0.2 | NO |
82 | PPIA | PPIA | PPIA | 11451 | -0.023 | 0.2 | NO |
83 | RAN | RAN | RAN | 11817 | -0.028 | 0.18 | NO |
84 | SEH1L | SEH1L | SEH1L | 11830 | -0.028 | 0.18 | NO |
85 | TCEB1 | TCEB1 | TCEB1 | 11865 | -0.028 | 0.18 | NO |
86 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.18 | NO |
87 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.18 | NO |
88 | AAAS | AAAS | AAAS | 12126 | -0.031 | 0.18 | NO |
89 | RANGAP1 | RANGAP1 | RANGAP1 | 12179 | -0.032 | 0.18 | NO |
90 | RAC1 | RAC1 | RAC1 | 12199 | -0.032 | 0.18 | NO |
91 | AP1M1 | AP1M1 | AP1M1 | 12284 | -0.033 | 0.18 | NO |
92 | AP2A1 | AP2A1 | AP2A1 | 12401 | -0.035 | 0.17 | NO |
93 | ARF1 | ARF1 | ARF1 | 12537 | -0.036 | 0.17 | NO |
94 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.17 | NO |
95 | BANF1 | BANF1 | BANF1 | 12672 | -0.038 | 0.17 | NO |
96 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.17 | NO |
97 | NUP188 | NUP188 | NUP188 | 12937 | -0.041 | 0.16 | NO |
98 | NUP214 | NUP214 | NUP214 | 13016 | -0.042 | 0.16 | NO |
99 | TCEB2 | TCEB2 | TCEB2 | 13126 | -0.043 | 0.16 | NO |
100 | POM121 | POM121 | POM121 | 13131 | -0.044 | 0.16 | NO |
101 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.16 | NO |
102 | SLC25A6 | SLC25A6 | SLC25A6 | 13306 | -0.046 | 0.16 | NO |
103 | NUP93 | NUP93 | NUP93 | 13355 | -0.046 | 0.16 | NO |
104 | NUPL1 | NUPL1 | NUPL1 | 13359 | -0.046 | 0.16 | NO |
105 | NPM1 | NPM1 | NPM1 | 13526 | -0.049 | 0.16 | NO |
106 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.14 | NO |
107 | AP2S1 | AP2S1 | AP2S1 | 13953 | -0.055 | 0.15 | NO |
108 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.14 | NO |
109 | RCC1 | RCC1 | RCC1 | 14146 | -0.058 | 0.14 | NO |
110 | NUP35 | NUP35 | NUP35 | 14497 | -0.064 | 0.13 | NO |
111 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.13 | NO |
112 | NUP133 | NUP133 | NUP133 | 14673 | -0.068 | 0.13 | NO |
113 | HMGA1 | HMGA1 | HMGA1 | 14722 | -0.069 | 0.14 | NO |
114 | AP1S1 | AP1S1 | AP1S1 | 14789 | -0.07 | 0.14 | NO |
115 | RAE1 | RAE1 | RAE1 | 14905 | -0.072 | 0.14 | NO |
116 | NUP155 | NUP155 | NUP155 | 15023 | -0.074 | 0.14 | NO |
117 | RANBP1 | RANBP1 | RANBP1 | 15538 | -0.086 | 0.12 | NO |
118 | CCNT1 | CCNT1 | CCNT1 | 15540 | -0.086 | 0.12 | NO |
119 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.13 | NO |
120 | AP1M2 | AP1M2 | AP1M2 | 15799 | -0.093 | 0.12 | NO |
Figure S21. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG GLYCOLYSIS GLUCONEOGENESIS.

Figure S22. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG GLYCOLYSIS GLUCONEOGENESIS, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S12. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CD247 | CD247 | CD247 | 104 | 0.87 | 0.05 | YES |
2 | CD8B | CD8B | CD8B | 129 | 0.85 | 0.1 | YES |
3 | LCK | LCK | LCK | 143 | 0.84 | 0.16 | YES |
4 | CD28 | CD28 | CD28 | 232 | 0.78 | 0.2 | YES |
5 | CCR5 | CCR5 | CCR5 | 361 | 0.69 | 0.24 | YES |
6 | DOCK2 | DOCK2 | DOCK2 | 431 | 0.66 | 0.28 | YES |
7 | HCK | HCK | HCK | 648 | 0.56 | 0.3 | YES |
8 | APOBEC3G | APOBEC3G | APOBEC3G | 850 | 0.49 | 0.32 | YES |
9 | ELMO1 | ELMO1 | ELMO1 | 901 | 0.47 | 0.35 | YES |
10 | CD4 | CD4 | CD4 | 926 | 0.47 | 0.38 | YES |
11 | CXCR4 | CXCR4 | CXCR4 | 1020 | 0.44 | 0.4 | YES |
12 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.43 | YES |
13 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.42 | YES |
14 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.43 | YES |
15 | NUP210 | NUP210 | NUP210 | 1833 | 0.27 | 0.44 | YES |
16 | B2M | B2M | B2M | 2091 | 0.24 | 0.44 | YES |
17 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.45 | YES |
18 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.46 | YES |
19 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.45 | YES |
20 | HLA-A | HLA-A | HLA-A | 2779 | 0.17 | 0.46 | YES |
21 | RNGTT | RNGTT | RNGTT | 3827 | 0.11 | 0.4 | NO |
22 | FYN | FYN | FYN | 3958 | 0.1 | 0.4 | NO |
23 | NMT2 | NMT2 | NMT2 | 4168 | 0.096 | 0.4 | NO |
24 | PSIP1 | PSIP1 | PSIP1 | 4484 | 0.085 | 0.38 | NO |
25 | LIG4 | LIG4 | LIG4 | 4650 | 0.08 | 0.38 | NO |
26 | TAF5 | TAF5 | TAF5 | 4746 | 0.077 | 0.38 | NO |
27 | PACS1 | PACS1 | PACS1 | 4869 | 0.073 | 0.38 | NO |
28 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.37 | NO |
29 | GTF2H2 | GTF2H2 | GTF2H2 | 5118 | 0.067 | 0.37 | NO |
30 | XRCC4 | XRCC4 | XRCC4 | 5215 | 0.064 | 0.37 | NO |
31 | ELL | ELL | ELL | 5521 | 0.058 | 0.36 | NO |
32 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.35 | NO |
33 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.34 | NO |
34 | CCNT2 | CCNT2 | CCNT2 | 6060 | 0.046 | 0.34 | NO |
35 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.33 | NO |
36 | GTF2E2 | GTF2E2 | GTF2E2 | 6275 | 0.042 | 0.33 | NO |
37 | GTF2E1 | GTF2E1 | GTF2E1 | 6291 | 0.042 | 0.33 | NO |
38 | GTF2B | GTF2B | GTF2B | 6452 | 0.039 | 0.33 | NO |
39 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.33 | NO |
40 | AP1B1 | AP1B1 | AP1B1 | 6502 | 0.038 | 0.33 | NO |
41 | GTF2H2B | GTF2H2B | GTF2H2B | 6617 | 0.036 | 0.33 | NO |
42 | CUL5 | CUL5 | CUL5 | 6669 | 0.035 | 0.32 | NO |
43 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.33 | NO |
44 | NUP54 | NUP54 | NUP54 | 6877 | 0.032 | 0.32 | NO |
45 | AP1S2 | AP1S2 | AP1S2 | 6890 | 0.031 | 0.32 | NO |
46 | NUPL2 | NUPL2 | NUPL2 | 6895 | 0.031 | 0.32 | NO |
47 | TBP | TBP | TBP | 6936 | 0.031 | 0.32 | NO |
48 | PAK2 | PAK2 | PAK2 | 6974 | 0.03 | 0.32 | NO |
49 | NUP43 | NUP43 | NUP43 | 7183 | 0.027 | 0.31 | NO |
50 | TAF12 | TAF12 | TAF12 | 7255 | 0.026 | 0.31 | NO |
51 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.31 | NO |
52 | TAF10 | TAF10 | TAF10 | 7396 | 0.024 | 0.3 | NO |
53 | TAF1 | TAF1 | TAF1 | 7448 | 0.023 | 0.3 | NO |
54 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.3 | NO |
55 | CDK9 | CDK9 | CDK9 | 7538 | 0.022 | 0.3 | NO |
56 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.3 | NO |
57 | RANBP2 | RANBP2 | RANBP2 | 7645 | 0.02 | 0.3 | NO |
58 | CTDP1 | CTDP1 | CTDP1 | 7744 | 0.019 | 0.29 | NO |
59 | RBX1 | RBX1 | RBX1 | 7930 | 0.017 | 0.28 | NO |
60 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.28 | NO |
61 | SUPT4H1 | SUPT4H1 | SUPT4H1 | 8057 | 0.015 | 0.28 | NO |
62 | CCNH | CCNH | CCNH | 8112 | 0.014 | 0.28 | NO |
63 | NUP37 | NUP37 | NUP37 | 8141 | 0.014 | 0.28 | NO |
64 | AP1G1 | AP1G1 | AP1G1 | 8253 | 0.013 | 0.27 | NO |
65 | VPS37A | VPS37A | VPS37A | 8304 | 0.012 | 0.27 | NO |
66 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.26 | NO |
67 | NUP62 | NUP62 | NUP62 | 8448 | 0.0099 | 0.26 | NO |
68 | NUP88 | NUP88 | NUP88 | 8494 | 0.0093 | 0.26 | NO |
69 | NUP50 | NUP50 | NUP50 | 8627 | 0.0078 | 0.25 | NO |
70 | NMT1 | NMT1 | NMT1 | 8643 | 0.0076 | 0.25 | NO |
71 | TAF9 | TAF9 | TAF9 | 8787 | 0.0056 | 0.24 | NO |
72 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.24 | NO |
73 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.24 | NO |
74 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.24 | NO |
75 | TPR | TPR | TPR | 9011 | 0.0029 | 0.23 | NO |
76 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.23 | NO |
77 | XPO1 | XPO1 | XPO1 | 9118 | 0.0016 | 0.23 | NO |
78 | CDK7 | CDK7 | CDK7 | 9225 | 0.00041 | 0.22 | NO |
79 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.22 | NO |
80 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.22 | NO |
81 | TCEA1 | TCEA1 | TCEA1 | 9283 | -0.00024 | 0.22 | NO |
82 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.21 | NO |
83 | GTF2A2 | GTF2A2 | GTF2A2 | 9500 | -0.0029 | 0.21 | NO |
84 | GTF2H1 | GTF2H1 | GTF2H1 | 9529 | -0.0031 | 0.21 | NO |
85 | NUP107 | NUP107 | NUP107 | 9601 | -0.0038 | 0.2 | NO |
86 | NUP85 | NUP85 | NUP85 | 9681 | -0.0047 | 0.2 | NO |
87 | TCEB3 | TCEB3 | TCEB3 | 9684 | -0.0047 | 0.2 | NO |
88 | ATP6V1H | ATP6V1H | ATP6V1H | 9799 | -0.0057 | 0.19 | NO |
89 | POLR2A | POLR2A | POLR2A | 9914 | -0.0071 | 0.19 | NO |
90 | NUP153 | NUP153 | NUP153 | 10004 | -0.0081 | 0.18 | NO |
91 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.18 | NO |
92 | POLR2K | POLR2K | POLR2K | 10086 | -0.0091 | 0.18 | NO |
93 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.18 | NO |
94 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.18 | NO |
95 | TAF4B | TAF4B | TAF4B | 10134 | -0.0096 | 0.18 | NO |
96 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.18 | NO |
97 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.18 | NO |
98 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.17 | NO |
99 | POLR2G | POLR2G | POLR2G | 10295 | -0.011 | 0.17 | NO |
100 | LIG1 | LIG1 | LIG1 | 10304 | -0.011 | 0.17 | NO |
101 | KPNA1 | KPNA1 | KPNA1 | 10399 | -0.012 | 0.17 | NO |
102 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.16 | NO |
103 | TAF4 | TAF4 | TAF4 | 10581 | -0.014 | 0.16 | NO |
104 | POLR2B | POLR2B | POLR2B | 10589 | -0.014 | 0.16 | NO |
105 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.16 | NO |
106 | POLR2J | POLR2J | POLR2J | 10668 | -0.015 | 0.16 | NO |
107 | RNMT | RNMT | RNMT | 10679 | -0.015 | 0.16 | NO |
108 | KPNB1 | KPNB1 | KPNB1 | 10689 | -0.015 | 0.16 | NO |
109 | AP2A2 | AP2A2 | AP2A2 | 10743 | -0.016 | 0.16 | NO |
110 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.16 | NO |
111 | AP2B1 | AP2B1 | AP2B1 | 10766 | -0.016 | 0.16 | NO |
112 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.16 | NO |
113 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.15 | NO |
114 | SLC25A4 | SLC25A4 | SLC25A4 | 11033 | -0.019 | 0.15 | NO |
115 | NCBP2 | NCBP2 | NCBP2 | 11072 | -0.02 | 0.14 | NO |
116 | SLC25A5 | SLC25A5 | SLC25A5 | 11136 | -0.02 | 0.14 | NO |
117 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.14 | NO |
118 | GTF2A1 | GTF2A1 | GTF2A1 | 11189 | -0.021 | 0.14 | NO |
119 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.14 | NO |
120 | AP2M1 | AP2M1 | AP2M1 | 11260 | -0.021 | 0.14 | NO |
121 | TAF11 | TAF11 | TAF11 | 11339 | -0.022 | 0.14 | NO |
122 | NUP205 | NUP205 | NUP205 | 11382 | -0.022 | 0.14 | NO |
123 | PPIA | PPIA | PPIA | 11451 | -0.023 | 0.14 | NO |
124 | TSG101 | TSG101 | TSG101 | 11583 | -0.025 | 0.13 | NO |
125 | TAF6 | TAF6 | TAF6 | 11748 | -0.027 | 0.12 | NO |
126 | VPS28 | VPS28 | VPS28 | 11789 | -0.027 | 0.12 | NO |
127 | RAN | RAN | RAN | 11817 | -0.028 | 0.12 | NO |
128 | SEH1L | SEH1L | SEH1L | 11830 | -0.028 | 0.12 | NO |
129 | POLR2C | POLR2C | POLR2C | 11858 | -0.028 | 0.12 | NO |
130 | XRCC6 | XRCC6 | XRCC6 | 11860 | -0.028 | 0.12 | NO |
131 | TCEB1 | TCEB1 | TCEB1 | 11865 | -0.028 | 0.13 | NO |
132 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.12 | NO |
133 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.12 | NO |
134 | ERCC3 | ERCC3 | ERCC3 | 12098 | -0.031 | 0.12 | NO |
135 | FEN1 | FEN1 | FEN1 | 12117 | -0.031 | 0.12 | NO |
136 | AAAS | AAAS | AAAS | 12126 | -0.031 | 0.12 | NO |
137 | RANGAP1 | RANGAP1 | RANGAP1 | 12179 | -0.032 | 0.12 | NO |
138 | RAC1 | RAC1 | RAC1 | 12199 | -0.032 | 0.12 | NO |
139 | AP1M1 | AP1M1 | AP1M1 | 12284 | -0.033 | 0.12 | NO |
140 | XRCC5 | XRCC5 | XRCC5 | 12287 | -0.033 | 0.12 | NO |
141 | VPS37C | VPS37C | VPS37C | 12336 | -0.034 | 0.12 | NO |
142 | POLR2L | POLR2L | POLR2L | 12370 | -0.034 | 0.12 | NO |
143 | AP2A1 | AP2A1 | AP2A1 | 12401 | -0.035 | 0.12 | NO |
144 | ARF1 | ARF1 | ARF1 | 12537 | -0.036 | 0.12 | NO |
145 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.12 | NO |
146 | GTF2H3 | GTF2H3 | GTF2H3 | 12630 | -0.037 | 0.12 | NO |
147 | GTF2F2 | GTF2F2 | GTF2F2 | 12640 | -0.037 | 0.12 | NO |
148 | BANF1 | BANF1 | BANF1 | 12672 | -0.038 | 0.12 | NO |
149 | POLR2H | POLR2H | POLR2H | 12673 | -0.038 | 0.12 | NO |
150 | COBRA1 | COBRA1 | COBRA1 | 12726 | -0.038 | 0.12 | NO |
151 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.12 | NO |
152 | SUPT16H | SUPT16H | SUPT16H | 12886 | -0.04 | 0.12 | NO |
153 | SUPT5H | SUPT5H | SUPT5H | 12925 | -0.041 | 0.12 | NO |
154 | RDBP | RDBP | RDBP | 12926 | -0.041 | 0.12 | NO |
155 | NUP188 | NUP188 | NUP188 | 12937 | -0.041 | 0.12 | NO |
156 | NUP214 | NUP214 | NUP214 | 13016 | -0.042 | 0.12 | NO |
157 | MNAT1 | MNAT1 | MNAT1 | 13074 | -0.043 | 0.12 | NO |
158 | POLR2D | POLR2D | POLR2D | 13098 | -0.043 | 0.12 | NO |
159 | TCEB2 | TCEB2 | TCEB2 | 13126 | -0.043 | 0.12 | NO |
160 | POM121 | POM121 | POM121 | 13131 | -0.044 | 0.13 | NO |
161 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.13 | NO |
162 | POLR2E | POLR2E | POLR2E | 13219 | -0.045 | 0.13 | NO |
163 | NCBP1 | NCBP1 | NCBP1 | 13272 | -0.045 | 0.13 | NO |
164 | SLC25A6 | SLC25A6 | SLC25A6 | 13306 | -0.046 | 0.13 | NO |
165 | NUP93 | NUP93 | NUP93 | 13355 | -0.046 | 0.13 | NO |
166 | NUPL1 | NUPL1 | NUPL1 | 13359 | -0.046 | 0.13 | NO |
167 | NPM1 | NPM1 | NPM1 | 13526 | -0.049 | 0.12 | NO |
168 | VPS37B | VPS37B | VPS37B | 13553 | -0.049 | 0.13 | NO |
169 | GTF2H4 | GTF2H4 | GTF2H4 | 13635 | -0.05 | 0.13 | NO |
170 | WHSC2 | WHSC2 | WHSC2 | 13854 | -0.054 | 0.12 | NO |
171 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.12 | NO |
172 | TAF13 | TAF13 | TAF13 | 13897 | -0.054 | 0.12 | NO |
173 | AP2S1 | AP2S1 | AP2S1 | 13953 | -0.055 | 0.12 | NO |
174 | SSRP1 | SSRP1 | SSRP1 | 14014 | -0.056 | 0.12 | NO |
175 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.12 | NO |
176 | TH1L | TH1L | TH1L | 14136 | -0.058 | 0.12 | NO |
177 | RCC1 | RCC1 | RCC1 | 14146 | -0.058 | 0.13 | NO |
178 | NUP35 | NUP35 | NUP35 | 14497 | -0.064 | 0.11 | NO |
179 | GTF2F1 | GTF2F1 | GTF2F1 | 14501 | -0.065 | 0.12 | NO |
180 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.12 | NO |
181 | NUP133 | NUP133 | NUP133 | 14673 | -0.068 | 0.11 | NO |
182 | HMGA1 | HMGA1 | HMGA1 | 14722 | -0.069 | 0.12 | NO |
183 | AP1S1 | AP1S1 | AP1S1 | 14789 | -0.07 | 0.12 | NO |
184 | POLR2F | POLR2F | POLR2F | 14892 | -0.072 | 0.12 | NO |
185 | RAE1 | RAE1 | RAE1 | 14905 | -0.072 | 0.12 | NO |
186 | NUP155 | NUP155 | NUP155 | 15023 | -0.074 | 0.12 | NO |
187 | ERCC2 | ERCC2 | ERCC2 | 15477 | -0.084 | 0.098 | NO |
188 | RANBP1 | RANBP1 | RANBP1 | 15538 | -0.086 | 0.1 | NO |
189 | CCNT1 | CCNT1 | CCNT1 | 15540 | -0.086 | 0.1 | NO |
190 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.11 | NO |
191 | AP1M2 | AP1M2 | AP1M2 | 15799 | -0.093 | 0.1 | NO |
192 | POLR2I | POLR2I | POLR2I | 16417 | -0.11 | 0.075 | NO |
193 | VPS37D | VPS37D | VPS37D | 17850 | -0.24 | 0.011 | NO |
Figure S23. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG FATTY ACID METABOLISM.

Figure S24. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG FATTY ACID METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S13. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CD19 | CD19 | CD19 | 32 | 0.96 | 0.054 | YES |
2 | CD79A | CD79A | CD79A | 90 | 0.89 | 0.1 | YES |
3 | BLK | BLK | BLK | 96 | 0.88 | 0.15 | YES |
4 | BLNK | BLNK | BLNK | 114 | 0.87 | 0.2 | YES |
5 | CARD11 | CARD11 | CARD11 | 131 | 0.84 | 0.25 | YES |
6 | RASGRP1 | RASGRP1 | RASGRP1 | 266 | 0.75 | 0.29 | YES |
7 | VAV1 | VAV1 | VAV1 | 288 | 0.74 | 0.33 | YES |
8 | CD79B | CD79B | CD79B | 339 | 0.71 | 0.37 | YES |
9 | PRKCB | PRKCB | PRKCB | 348 | 0.7 | 0.41 | YES |
10 | BTK | BTK | BTK | 387 | 0.68 | 0.44 | YES |
11 | PIK3AP1 | PIK3AP1 | PIK3AP1 | 587 | 0.58 | 0.47 | YES |
12 | PLCG2 | PLCG2 | PLCG2 | 760 | 0.52 | 0.49 | YES |
13 | SYK | SYK | SYK | 771 | 0.52 | 0.52 | YES |
14 | LYN | LYN | LYN | 1007 | 0.44 | 0.53 | YES |
15 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.55 | YES |
16 | RASGRP3 | RASGRP3 | RASGRP3 | 1060 | 0.43 | 0.58 | YES |
17 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.57 | YES |
18 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.57 | YES |
19 | NFKBIA | NFKBIA | NFKBIA | 2005 | 0.25 | 0.57 | YES |
20 | REL | REL | REL | 2106 | 0.23 | 0.58 | YES |
21 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.59 | YES |
22 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.59 | YES |
23 | PIK3CD | PIK3CD | PIK3CD | 2454 | 0.2 | 0.6 | YES |
24 | MALT1 | MALT1 | MALT1 | 2679 | 0.18 | 0.6 | YES |
25 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.61 | YES |
26 | CBLB | CBLB | CBLB | 3062 | 0.15 | 0.6 | NO |
27 | NFKBIE | NFKBIE | NFKBIE | 3335 | 0.13 | 0.59 | NO |
28 | NCK1 | NCK1 | NCK1 | 3419 | 0.13 | 0.59 | NO |
29 | CDKN1B | CDKN1B | CDKN1B | 3481 | 0.12 | 0.59 | NO |
30 | ITPR2 | ITPR2 | ITPR2 | 3498 | 0.12 | 0.6 | NO |
31 | GRB2 | GRB2 | GRB2 | 3813 | 0.11 | 0.59 | NO |
32 | FYN | FYN | FYN | 3958 | 0.1 | 0.59 | NO |
33 | PTEN | PTEN | PTEN | 4081 | 0.099 | 0.59 | NO |
34 | FOXO1 | FOXO1 | FOXO1 | 4483 | 0.085 | 0.57 | NO |
35 | RICTOR | RICTOR | RICTOR | 4614 | 0.081 | 0.57 | NO |
36 | PIK3R1 | PIK3R1 | PIK3R1 | 4687 | 0.078 | 0.57 | NO |
37 | IKBKG | IKBKG | IKBKG | 4881 | 0.073 | 0.56 | NO |
38 | BCL10 | BCL10 | BCL10 | 4930 | 0.072 | 0.56 | NO |
39 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.56 | NO |
40 | SOS1 | SOS1 | SOS1 | 5293 | 0.062 | 0.55 | NO |
41 | MAP3K7 | MAP3K7 | MAP3K7 | 5313 | 0.062 | 0.55 | NO |
42 | CREB1 | CREB1 | CREB1 | 5420 | 0.06 | 0.55 | NO |
43 | CBL | CBL | CBL | 5456 | 0.059 | 0.55 | NO |
44 | ORAI1 | ORAI1 | ORAI1 | 5481 | 0.059 | 0.55 | NO |
45 | SH3KBP1 | SH3KBP1 | SH3KBP1 | 5570 | 0.057 | 0.55 | NO |
46 | CHUK | CHUK | CHUK | 5614 | 0.056 | 0.55 | NO |
47 | IKBKB | IKBKB | IKBKB | 5656 | 0.055 | 0.55 | NO |
48 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.56 | NO |
49 | KRAS | KRAS | KRAS | 5737 | 0.053 | 0.55 | NO |
50 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.55 | NO |
51 | NFKBIB | NFKBIB | NFKBIB | 6019 | 0.048 | 0.54 | NO |
52 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.54 | NO |
53 | ITPR3 | ITPR3 | ITPR3 | 6390 | 0.04 | 0.53 | NO |
54 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.53 | NO |
55 | STIM1 | STIM1 | STIM1 | 6657 | 0.035 | 0.52 | NO |
56 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.52 | NO |
57 | NRAS | NRAS | NRAS | 7125 | 0.028 | 0.5 | NO |
58 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.49 | NO |
59 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.48 | NO |
60 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.47 | NO |
61 | CALM1 | CALM1 | CALM1 | 7658 | 0.02 | 0.47 | NO |
62 | CALM2 | CALM2 | CALM2 | 7817 | 0.018 | 0.46 | NO |
63 | CUL1 | CUL1 | CUL1 | 7889 | 0.017 | 0.46 | NO |
64 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.46 | NO |
65 | FOXO3 | FOXO3 | FOXO3 | 8268 | 0.012 | 0.44 | NO |
66 | CASP9 | CASP9 | CASP9 | 8352 | 0.011 | 0.44 | NO |
67 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.44 | NO |
68 | FOXO4 | FOXO4 | FOXO4 | 8534 | 0.0089 | 0.43 | NO |
69 | RPS6KB2 | RPS6KB2 | RPS6KB2 | 8543 | 0.0087 | 0.43 | NO |
70 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.41 | NO |
71 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.41 | NO |
72 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.4 | NO |
73 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.4 | NO |
74 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.39 | NO |
75 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.39 | NO |
76 | CALM3 | CALM3 | CALM3 | 9278 | -0.00018 | 0.39 | NO |
77 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.38 | NO |
78 | MDM2 | MDM2 | MDM2 | 9895 | -0.0068 | 0.36 | NO |
79 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.35 | NO |
80 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.34 | NO |
81 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.34 | NO |
82 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.34 | NO |
83 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.34 | NO |
84 | MTOR | MTOR | MTOR | 10211 | -0.01 | 0.34 | NO |
85 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.34 | NO |
86 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.32 | NO |
87 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.32 | NO |
88 | PDPK1 | PDPK1 | PDPK1 | 10699 | -0.015 | 0.32 | NO |
89 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.32 | NO |
90 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.31 | NO |
91 | MAPKAP1 | MAPKAP1 | MAPKAP1 | 10878 | -0.018 | 0.31 | NO |
92 | RELA | RELA | RELA | 10903 | -0.018 | 0.31 | NO |
93 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.31 | NO |
94 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.3 | NO |
95 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.3 | NO |
96 | THEM4 | THEM4 | THEM4 | 11698 | -0.026 | 0.27 | NO |
97 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.25 | NO |
98 | PLCG1 | PLCG1 | PLCG1 | 12039 | -0.03 | 0.26 | NO |
99 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.26 | NO |
100 | AKT1 | AKT1 | AKT1 | 12293 | -0.033 | 0.24 | NO |
101 | FBXW11 | FBXW11 | FBXW11 | 12300 | -0.033 | 0.25 | NO |
102 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.23 | NO |
103 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.23 | NO |
104 | BAD | BAD | BAD | 12804 | -0.039 | 0.22 | NO |
105 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.21 | NO |
106 | AKT2 | AKT2 | AKT2 | 13293 | -0.046 | 0.2 | NO |
107 | GSK3A | GSK3A | GSK3A | 13654 | -0.05 | 0.19 | NO |
108 | AKT3 | AKT3 | AKT3 | 13795 | -0.053 | 0.18 | NO |
109 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.18 | NO |
110 | TSC2 | TSC2 | TSC2 | 13917 | -0.055 | 0.18 | NO |
111 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.18 | NO |
112 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.15 | NO |
113 | CDKN1A | CDKN1A | CDKN1A | 14662 | -0.068 | 0.15 | NO |
114 | SHC1 | SHC1 | SHC1 | 14751 | -0.069 | 0.15 | NO |
115 | NR4A1 | NR4A1 | NR4A1 | 14797 | -0.07 | 0.15 | NO |
116 | PHLPP1 | PHLPP1 | PHLPP1 | 15049 | -0.075 | 0.14 | NO |
117 | MLST8 | MLST8 | MLST8 | 15368 | -0.081 | 0.13 | NO |
118 | TRIB3 | TRIB3 | TRIB3 | 15399 | -0.082 | 0.13 | NO |
119 | AKT1S1 | AKT1S1 | AKT1S1 | 15555 | -0.086 | 0.13 | NO |
120 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.13 | NO |
121 | HRAS | HRAS | HRAS | 16012 | -0.1 | 0.11 | NO |
Figure S25. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG ALANINE ASPARTATE AND GLUTAMATE METABOLISM.

Figure S26. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG ALANINE ASPARTATE AND GLUTAMATE METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S14. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CARD11 | CARD11 | CARD11 | 131 | 0.84 | 0.097 | YES |
2 | RASGRP1 | RASGRP1 | RASGRP1 | 266 | 0.75 | 0.18 | YES |
3 | PRKCB | PRKCB | PRKCB | 348 | 0.7 | 0.26 | YES |
4 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.28 | YES |
5 | RASGRP3 | RASGRP3 | RASGRP3 | 1060 | 0.43 | 0.33 | YES |
6 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.34 | YES |
7 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.37 | YES |
8 | NFKBIA | NFKBIA | NFKBIA | 2005 | 0.25 | 0.38 | YES |
9 | REL | REL | REL | 2106 | 0.23 | 0.41 | YES |
10 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.43 | YES |
11 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.44 | YES |
12 | MALT1 | MALT1 | MALT1 | 2679 | 0.18 | 0.45 | YES |
13 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.47 | YES |
14 | NFKBIE | NFKBIE | NFKBIE | 3335 | 0.13 | 0.45 | NO |
15 | CDKN1B | CDKN1B | CDKN1B | 3481 | 0.12 | 0.46 | NO |
16 | PTEN | PTEN | PTEN | 4081 | 0.099 | 0.44 | NO |
17 | FOXO1 | FOXO1 | FOXO1 | 4483 | 0.085 | 0.43 | NO |
18 | RICTOR | RICTOR | RICTOR | 4614 | 0.081 | 0.43 | NO |
19 | IKBKG | IKBKG | IKBKG | 4881 | 0.073 | 0.42 | NO |
20 | BCL10 | BCL10 | BCL10 | 4930 | 0.072 | 0.43 | NO |
21 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.43 | NO |
22 | MAP3K7 | MAP3K7 | MAP3K7 | 5313 | 0.062 | 0.42 | NO |
23 | CREB1 | CREB1 | CREB1 | 5420 | 0.06 | 0.43 | NO |
24 | CHUK | CHUK | CHUK | 5614 | 0.056 | 0.42 | NO |
25 | IKBKB | IKBKB | IKBKB | 5656 | 0.055 | 0.43 | NO |
26 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.43 | NO |
27 | KRAS | KRAS | KRAS | 5737 | 0.053 | 0.44 | NO |
28 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.43 | NO |
29 | NFKBIB | NFKBIB | NFKBIB | 6019 | 0.048 | 0.43 | NO |
30 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.43 | NO |
31 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.42 | NO |
32 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.41 | NO |
33 | NRAS | NRAS | NRAS | 7125 | 0.028 | 0.39 | NO |
34 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.38 | NO |
35 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.37 | NO |
36 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.37 | NO |
37 | CUL1 | CUL1 | CUL1 | 7889 | 0.017 | 0.36 | NO |
38 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.35 | NO |
39 | FOXO3 | FOXO3 | FOXO3 | 8268 | 0.012 | 0.34 | NO |
40 | CASP9 | CASP9 | CASP9 | 8352 | 0.011 | 0.34 | NO |
41 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.34 | NO |
42 | FOXO4 | FOXO4 | FOXO4 | 8534 | 0.0089 | 0.33 | NO |
43 | RPS6KB2 | RPS6KB2 | RPS6KB2 | 8543 | 0.0087 | 0.33 | NO |
44 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.32 | NO |
45 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.31 | NO |
46 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.31 | NO |
47 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.3 | NO |
48 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.29 | NO |
49 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.29 | NO |
50 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.28 | NO |
51 | MDM2 | MDM2 | MDM2 | 9895 | -0.0068 | 0.26 | NO |
52 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.25 | NO |
53 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.25 | NO |
54 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.25 | NO |
55 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.25 | NO |
56 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.25 | NO |
57 | MTOR | MTOR | MTOR | 10211 | -0.01 | 0.25 | NO |
58 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.25 | NO |
59 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.23 | NO |
60 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.23 | NO |
61 | PDPK1 | PDPK1 | PDPK1 | 10699 | -0.015 | 0.23 | NO |
62 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.22 | NO |
63 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.22 | NO |
64 | MAPKAP1 | MAPKAP1 | MAPKAP1 | 10878 | -0.018 | 0.22 | NO |
65 | RELA | RELA | RELA | 10903 | -0.018 | 0.22 | NO |
66 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.22 | NO |
67 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.22 | NO |
68 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.22 | NO |
69 | THEM4 | THEM4 | THEM4 | 11698 | -0.026 | 0.19 | NO |
70 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.18 | NO |
71 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.18 | NO |
72 | AKT1 | AKT1 | AKT1 | 12293 | -0.033 | 0.17 | NO |
73 | FBXW11 | FBXW11 | FBXW11 | 12300 | -0.033 | 0.17 | NO |
74 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.16 | NO |
75 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.16 | NO |
76 | BAD | BAD | BAD | 12804 | -0.039 | 0.16 | NO |
77 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.15 | NO |
78 | AKT2 | AKT2 | AKT2 | 13293 | -0.046 | 0.14 | NO |
79 | GSK3A | GSK3A | GSK3A | 13654 | -0.05 | 0.13 | NO |
80 | AKT3 | AKT3 | AKT3 | 13795 | -0.053 | 0.13 | NO |
81 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.13 | NO |
82 | TSC2 | TSC2 | TSC2 | 13917 | -0.055 | 0.13 | NO |
83 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.13 | NO |
84 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.11 | NO |
85 | CDKN1A | CDKN1A | CDKN1A | 14662 | -0.068 | 0.12 | NO |
86 | NR4A1 | NR4A1 | NR4A1 | 14797 | -0.07 | 0.12 | NO |
87 | PHLPP1 | PHLPP1 | PHLPP1 | 15049 | -0.075 | 0.11 | NO |
88 | MLST8 | MLST8 | MLST8 | 15368 | -0.081 | 0.1 | NO |
89 | TRIB3 | TRIB3 | TRIB3 | 15399 | -0.082 | 0.11 | NO |
90 | AKT1S1 | AKT1S1 | AKT1S1 | 15555 | -0.086 | 0.12 | NO |
91 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.12 | NO |
92 | HRAS | HRAS | HRAS | 16012 | -0.1 | 0.11 | NO |
Figure S27. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG GLYCINE SERINE AND THREONINE METABOLISM.

Figure S28. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG GLYCINE SERINE AND THREONINE METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S15. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | SOCS1 | SOCS1 | SOCS1 | 393 | 0.68 | 0.012 | YES |
2 | NCF4 | NCF4 | NCF4 | 497 | 0.62 | 0.037 | YES |
3 | FCGR1A | FCGR1A | FCGR1A | 623 | 0.57 | 0.058 | YES |
4 | FCGR1B | FCGR1B | FCGR1B | 626 | 0.57 | 0.086 | YES |
5 | CYBB | CYBB | CYBB | 677 | 0.55 | 0.11 | YES |
6 | NCF2 | NCF2 | NCF2 | 728 | 0.53 | 0.13 | YES |
7 | MRC1 | MRC1 | MRC1 | 977 | 0.45 | 0.14 | YES |
8 | CTSS | CTSS | CTSS | 981 | 0.45 | 0.16 | YES |
9 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.18 | YES |
10 | HLA-F | HLA-F | HLA-F | 1235 | 0.39 | 0.19 | YES |
11 | ASB2 | ASB2 | ASB2 | 1427 | 0.34 | 0.2 | YES |
12 | ASB12 | ASB12 | ASB12 | 1436 | 0.34 | 0.22 | YES |
13 | UBA7 | UBA7 | UBA7 | 1452 | 0.34 | 0.23 | YES |
14 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.24 | YES |
15 | FBXO6 | FBXO6 | FBXO6 | 1576 | 0.31 | 0.26 | YES |
16 | TAP1 | TAP1 | TAP1 | 1724 | 0.29 | 0.26 | YES |
17 | HLA-G | HLA-G | HLA-G | 1734 | 0.28 | 0.27 | YES |
18 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.29 | YES |
19 | HLA-B | HLA-B | HLA-B | 1796 | 0.27 | 0.3 | YES |
20 | UBE2L6 | UBE2L6 | UBE2L6 | 1840 | 0.27 | 0.31 | YES |
21 | CYBA | CYBA | CYBA | 1912 | 0.26 | 0.32 | YES |
22 | CD207 | CD207 | CD207 | 2057 | 0.24 | 0.32 | YES |
23 | TRIM21 | TRIM21 | TRIM21 | 2069 | 0.24 | 0.33 | YES |
24 | B2M | B2M | B2M | 2091 | 0.24 | 0.34 | YES |
25 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.35 | YES |
26 | HLA-C | HLA-C | HLA-C | 2224 | 0.22 | 0.36 | YES |
27 | SOCS3 | SOCS3 | SOCS3 | 2239 | 0.22 | 0.37 | YES |
28 | DTX3L | DTX3L | DTX3L | 2287 | 0.21 | 0.38 | YES |
29 | ERAP1 | ERAP1 | ERAP1 | 2325 | 0.21 | 0.38 | YES |
30 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.39 | YES |
31 | LNPEP | LNPEP | LNPEP | 2448 | 0.2 | 0.4 | YES |
32 | TAP2 | TAP2 | TAP2 | 2571 | 0.19 | 0.4 | YES |
33 | FBXO2 | FBXO2 | FBXO2 | 2574 | 0.19 | 0.41 | YES |
34 | UBE2E2 | UBE2E2 | UBE2E2 | 2584 | 0.19 | 0.42 | YES |
35 | ZBTB16 | ZBTB16 | ZBTB16 | 2634 | 0.18 | 0.42 | YES |
36 | RNF144B | RNF144B | RNF144B | 2704 | 0.18 | 0.43 | YES |
37 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.44 | YES |
38 | UBE2D1 | UBE2D1 | UBE2D1 | 2735 | 0.17 | 0.45 | YES |
39 | HLA-A | HLA-A | HLA-A | 2779 | 0.17 | 0.45 | YES |
40 | ASB16 | ASB16 | ASB16 | 2830 | 0.17 | 0.46 | YES |
41 | UBE2J1 | UBE2J1 | UBE2J1 | 2845 | 0.16 | 0.46 | YES |
42 | CBLB | CBLB | CBLB | 3062 | 0.15 | 0.46 | NO |
43 | FBXW7 | FBXW7 | FBXW7 | 3272 | 0.14 | 0.46 | NO |
44 | DZIP3 | DZIP3 | DZIP3 | 3515 | 0.12 | 0.45 | NO |
45 | SAR1B | SAR1B | SAR1B | 3817 | 0.11 | 0.44 | NO |
46 | FBXO27 | FBXO27 | FBXO27 | 4072 | 0.1 | 0.43 | NO |
47 | CD36 | CD36 | CD36 | 4096 | 0.099 | 0.43 | NO |
48 | PARK2 | PARK2 | PARK2 | 4211 | 0.094 | 0.43 | NO |
49 | UBE2F | UBE2F | UBE2F | 4429 | 0.086 | 0.42 | NO |
50 | WSB1 | WSB1 | WSB1 | 4506 | 0.084 | 0.42 | NO |
51 | CUL2 | CUL2 | CUL2 | 4523 | 0.083 | 0.42 | NO |
52 | ASB9 | ASB9 | ASB9 | 4668 | 0.079 | 0.42 | NO |
53 | WWP1 | WWP1 | WWP1 | 4806 | 0.075 | 0.42 | NO |
54 | UBA6 | UBA6 | UBA6 | 4818 | 0.074 | 0.42 | NO |
55 | SPSB2 | SPSB2 | SPSB2 | 4978 | 0.07 | 0.41 | NO |
56 | FBXO4 | FBXO4 | FBXO4 | 5026 | 0.069 | 0.41 | NO |
57 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.42 | NO |
58 | UBR1 | UBR1 | UBR1 | 5120 | 0.067 | 0.42 | NO |
59 | ASB8 | ASB8 | ASB8 | 5129 | 0.066 | 0.42 | NO |
60 | RNF138 | RNF138 | RNF138 | 5135 | 0.066 | 0.42 | NO |
61 | SEC24D | SEC24D | SEC24D | 5267 | 0.063 | 0.42 | NO |
62 | ANAPC10 | ANAPC10 | ANAPC10 | 5450 | 0.059 | 0.41 | NO |
63 | ANAPC4 | ANAPC4 | ANAPC4 | 5577 | 0.057 | 0.41 | NO |
64 | ATG7 | ATG7 | ATG7 | 5603 | 0.056 | 0.41 | NO |
65 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.41 | NO |
66 | UBE2A | UBE2A | UBE2A | 5755 | 0.053 | 0.4 | NO |
67 | UBA3 | UBA3 | UBA3 | 5879 | 0.05 | 0.4 | NO |
68 | RNF34 | RNF34 | RNF34 | 5886 | 0.05 | 0.4 | NO |
69 | ASB7 | ASB7 | ASB7 | 5934 | 0.049 | 0.4 | NO |
70 | KLHL9 | KLHL9 | KLHL9 | 5938 | 0.049 | 0.4 | NO |
71 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.4 | NO |
72 | UBE2Q2 | UBE2Q2 | UBE2Q2 | 5995 | 0.048 | 0.41 | NO |
73 | UBE2B | UBE2B | UBE2B | 6012 | 0.048 | 0.41 | NO |
74 | UBR2 | UBR2 | UBR2 | 6081 | 0.046 | 0.41 | NO |
75 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.4 | NO |
76 | VHL | VHL | VHL | 6311 | 0.042 | 0.4 | NO |
77 | RNF4 | RNF4 | RNF4 | 6443 | 0.039 | 0.39 | NO |
78 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.39 | NO |
79 | ITGAV | ITGAV | ITGAV | 6492 | 0.038 | 0.39 | NO |
80 | UBE2D2 | UBE2D2 | UBE2D2 | 6510 | 0.038 | 0.39 | NO |
81 | FBXO44 | FBXO44 | FBXO44 | 6580 | 0.036 | 0.39 | NO |
82 | CUL5 | CUL5 | CUL5 | 6669 | 0.035 | 0.39 | NO |
83 | UBE2D3 | UBE2D3 | UBE2D3 | 6675 | 0.035 | 0.39 | NO |
84 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.39 | NO |
85 | RBCK1 | RBCK1 | RBCK1 | 6708 | 0.034 | 0.39 | NO |
86 | TMEM189-UBE2V1 | TMEM189-UBE2V1 | TMEM189-UBE2V1 | 6752 | 0.034 | 0.39 | NO |
87 | SMURF2 | SMURF2 | SMURF2 | 6777 | 0.033 | 0.39 | NO |
88 | CDC26 | CDC26 | CDC26 | 6797 | 0.033 | 0.39 | NO |
89 | SPSB1 | SPSB1 | SPSB1 | 6856 | 0.032 | 0.39 | NO |
90 | CUL3 | CUL3 | CUL3 | 6861 | 0.032 | 0.39 | NO |
91 | PJA2 | PJA2 | PJA2 | 6924 | 0.031 | 0.39 | NO |
92 | UBE2W | UBE2W | UBE2W | 6958 | 0.03 | 0.39 | NO |
93 | UBE2G1 | UBE2G1 | UBE2G1 | 7003 | 0.029 | 0.39 | NO |
94 | RCHY1 | RCHY1 | RCHY1 | 7163 | 0.027 | 0.38 | NO |
95 | NEDD4 | NEDD4 | NEDD4 | 7208 | 0.026 | 0.38 | NO |
96 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.38 | NO |
97 | SEC61B | SEC61B | SEC61B | 7359 | 0.024 | 0.37 | NO |
98 | MKRN1 | MKRN1 | MKRN1 | 7425 | 0.023 | 0.37 | NO |
99 | ASB13 | ASB13 | ASB13 | 7444 | 0.023 | 0.37 | NO |
100 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.37 | NO |
101 | UBA5 | UBA5 | UBA5 | 7532 | 0.022 | 0.37 | NO |
102 | MRC2 | MRC2 | MRC2 | 7572 | 0.021 | 0.37 | NO |
103 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.37 | NO |
104 | TRIP12 | TRIP12 | TRIP12 | 7627 | 0.02 | 0.37 | NO |
105 | UBE4A | UBE4A | UBE4A | 7726 | 0.019 | 0.36 | NO |
106 | TRIM63 | TRIM63 | TRIM63 | 7887 | 0.017 | 0.35 | NO |
107 | CUL1 | CUL1 | CUL1 | 7889 | 0.017 | 0.36 | NO |
108 | RBX1 | RBX1 | RBX1 | 7930 | 0.017 | 0.35 | NO |
109 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.35 | NO |
110 | UBE2N | UBE2N | UBE2N | 8008 | 0.016 | 0.35 | NO |
111 | RNF6 | RNF6 | RNF6 | 8075 | 0.015 | 0.35 | NO |
112 | PDIA3 | PDIA3 | PDIA3 | 8111 | 0.014 | 0.35 | NO |
113 | UBE3A | UBE3A | UBE3A | 8260 | 0.012 | 0.34 | NO |
114 | UBE2Z | UBE2Z | UBE2Z | 8283 | 0.012 | 0.34 | NO |
115 | TRIM37 | TRIM37 | TRIM37 | 8315 | 0.012 | 0.34 | NO |
116 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.33 | NO |
117 | HECTD3 | HECTD3 | HECTD3 | 8422 | 0.01 | 0.33 | NO |
118 | FBXO3 | FBXO3 | FBXO3 | 8495 | 0.0093 | 0.33 | NO |
119 | VPRBP | VPRBP | VPRBP | 8640 | 0.0077 | 0.32 | NO |
120 | SPSB4 | SPSB4 | SPSB4 | 8696 | 0.0069 | 0.32 | NO |
121 | TPP2 | TPP2 | TPP2 | 8777 | 0.0058 | 0.31 | NO |
122 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.31 | NO |
123 | UBE3B | UBE3B | UBE3B | 8819 | 0.0053 | 0.31 | NO |
124 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.31 | NO |
125 | ANAPC5 | ANAPC5 | ANAPC5 | 8882 | 0.0045 | 0.31 | NO |
126 | CDC27 | CDC27 | CDC27 | 8930 | 0.0039 | 0.31 | NO |
127 | SEC24C | SEC24C | SEC24C | 8951 | 0.0036 | 0.31 | NO |
128 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.3 | NO |
129 | UBE2K | UBE2K | UBE2K | 8979 | 0.0033 | 0.3 | NO |
130 | UBE2H | UBE2H | UBE2H | 8989 | 0.0032 | 0.3 | NO |
131 | CDC23 | CDC23 | CDC23 | 9059 | 0.0024 | 0.3 | NO |
132 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.3 | NO |
133 | UBE2E1 | UBE2E1 | UBE2E1 | 9163 | 0.0011 | 0.3 | NO |
134 | UBE2G2 | UBE2G2 | UBE2G2 | 9241 | 0.00033 | 0.29 | NO |
135 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.29 | NO |
136 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.29 | NO |
137 | ANAPC2 | ANAPC2 | ANAPC2 | 9360 | -0.0013 | 0.28 | NO |
138 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.28 | NO |
139 | KEAP1 | KEAP1 | KEAP1 | 9621 | -0.004 | 0.27 | NO |
140 | SEC24B | SEC24B | SEC24B | 9767 | -0.0054 | 0.26 | NO |
141 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.25 | NO |
142 | ANAPC13 | ANAPC13 | ANAPC13 | 10050 | -0.0086 | 0.25 | NO |
143 | UBE2J2 | UBE2J2 | UBE2J2 | 10090 | -0.0091 | 0.24 | NO |
144 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.24 | NO |
145 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.24 | NO |
146 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.24 | NO |
147 | ANAPC7 | ANAPC7 | ANAPC7 | 10150 | -0.0098 | 0.24 | NO |
148 | UBE3C | UBE3C | UBE3C | 10187 | -0.01 | 0.24 | NO |
149 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.24 | NO |
150 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.24 | NO |
151 | UBE2R2 | UBE2R2 | UBE2R2 | 10277 | -0.011 | 0.24 | NO |
152 | RNF220 | RNF220 | RNF220 | 10282 | -0.011 | 0.24 | NO |
153 | ASB3 | ASB3 | ASB3 | 10332 | -0.012 | 0.24 | NO |
154 | NPEPPS | NPEPPS | NPEPPS | 10405 | -0.012 | 0.23 | NO |
155 | CALR | CALR | CALR | 10431 | -0.012 | 0.23 | NO |
156 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.23 | NO |
157 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.22 | NO |
158 | UBR4 | UBR4 | UBR4 | 10685 | -0.015 | 0.22 | NO |
159 | HUWE1 | HUWE1 | HUWE1 | 10693 | -0.015 | 0.22 | NO |
160 | ARIH2 | ARIH2 | ARIH2 | 10751 | -0.016 | 0.22 | NO |
161 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.22 | NO |
162 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.22 | NO |
163 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.21 | NO |
164 | KLHL20 | KLHL20 | KLHL20 | 11106 | -0.02 | 0.2 | NO |
165 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.2 | NO |
166 | LRSAM1 | LRSAM1 | LRSAM1 | 11150 | -0.02 | 0.2 | NO |
167 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.2 | NO |
168 | UBOX5 | UBOX5 | UBOX5 | 11224 | -0.021 | 0.2 | NO |
169 | UBE2V2 | UBE2V2 | UBE2V2 | 11348 | -0.022 | 0.2 | NO |
170 | UBE2L3 | UBE2L3 | UBE2L3 | 11373 | -0.022 | 0.2 | NO |
171 | CDC16 | CDC16 | CDC16 | 11442 | -0.023 | 0.19 | NO |
172 | SEC61A1 | SEC61A1 | SEC61A1 | 11538 | -0.024 | 0.19 | NO |
173 | SEC31A | SEC31A | SEC31A | 11554 | -0.025 | 0.19 | NO |
174 | PJA1 | PJA1 | PJA1 | 11727 | -0.027 | 0.18 | NO |
175 | CANX | CANX | CANX | 11751 | -0.027 | 0.18 | NO |
176 | SEC23A | SEC23A | SEC23A | 11761 | -0.027 | 0.18 | NO |
177 | ANAPC11 | ANAPC11 | ANAPC11 | 11842 | -0.028 | 0.18 | NO |
178 | RNF182 | RNF182 | RNF182 | 11850 | -0.028 | 0.18 | NO |
179 | TCEB1 | TCEB1 | TCEB1 | 11865 | -0.028 | 0.18 | NO |
180 | UBA1 | UBA1 | UBA1 | 11932 | -0.029 | 0.18 | NO |
181 | HSPA5 | HSPA5 | HSPA5 | 12012 | -0.03 | 0.17 | NO |
182 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.17 | NO |
183 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.17 | NO |
184 | SEC61G | SEC61G | SEC61G | 12071 | -0.03 | 0.18 | NO |
185 | SEC13 | SEC13 | SEC13 | 12153 | -0.032 | 0.17 | NO |
186 | FBXW11 | FBXW11 | FBXW11 | 12300 | -0.033 | 0.17 | NO |
187 | RNF41 | RNF41 | RNF41 | 12326 | -0.034 | 0.17 | NO |
188 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.15 | NO |
189 | BLMH | BLMH | BLMH | 12690 | -0.038 | 0.15 | NO |
190 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.15 | NO |
191 | SMURF1 | SMURF1 | SMURF1 | 12897 | -0.04 | 0.14 | NO |
192 | STUB1 | STUB1 | STUB1 | 12910 | -0.041 | 0.14 | NO |
193 | ASB11 | ASB11 | ASB11 | 13042 | -0.042 | 0.14 | NO |
194 | TCEB2 | TCEB2 | TCEB2 | 13126 | -0.043 | 0.14 | NO |
195 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.14 | NO |
196 | UBE2Q1 | UBE2Q1 | UBE2Q1 | 13165 | -0.044 | 0.14 | NO |
197 | RNF25 | RNF25 | RNF25 | 13175 | -0.044 | 0.14 | NO |
198 | UBE2O | UBE2O | UBE2O | 13214 | -0.045 | 0.14 | NO |
199 | TRIM36 | TRIM36 | TRIM36 | 13247 | -0.045 | 0.14 | NO |
200 | CDC20 | CDC20 | CDC20 | 13333 | -0.046 | 0.14 | NO |
201 | SEC61A2 | SEC61A2 | SEC61A2 | 13395 | -0.047 | 0.14 | NO |
202 | TRIM11 | TRIM11 | TRIM11 | 13458 | -0.048 | 0.14 | NO |
203 | SAE1 | SAE1 | SAE1 | 13588 | -0.05 | 0.13 | NO |
204 | UBE2D4 | UBE2D4 | UBE2D4 | 13725 | -0.052 | 0.13 | NO |
205 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.12 | NO |
206 | ASB6 | ASB6 | ASB6 | 13885 | -0.054 | 0.12 | NO |
207 | HERC2 | HERC2 | HERC2 | 13979 | -0.056 | 0.12 | NO |
208 | UBA2 | UBA2 | UBA2 | 14046 | -0.057 | 0.12 | NO |
209 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.12 | NO |
210 | ANAPC1 | ANAPC1 | ANAPC1 | 14151 | -0.058 | 0.12 | NO |
211 | RNF123 | RNF123 | RNF123 | 14230 | -0.06 | 0.12 | NO |
212 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.1 | NO |
213 | UBE2M | UBE2M | UBE2M | 14584 | -0.066 | 0.1 | NO |
214 | ASB4 | ASB4 | ASB4 | 14948 | -0.073 | 0.087 | NO |
215 | UBE2E3 | UBE2E3 | UBE2E3 | 14949 | -0.073 | 0.091 | NO |
216 | ASB1 | ASB1 | ASB1 | 15130 | -0.076 | 0.085 | NO |
217 | UBE2C | UBE2C | UBE2C | 15144 | -0.077 | 0.088 | NO |
218 | LRRC41 | LRRC41 | LRRC41 | 15291 | -0.08 | 0.083 | NO |
219 | FBXW8 | FBXW8 | FBXW8 | 15520 | -0.085 | 0.075 | NO |
220 | CDC34 | CDC34 | CDC34 | 15546 | -0.086 | 0.078 | NO |
221 | UBE2S | UBE2S | UBE2S | 15558 | -0.086 | 0.081 | NO |
222 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.085 | NO |
223 | DET1 | DET1 | DET1 | 15643 | -0.088 | 0.085 | NO |
224 | CUL7 | CUL7 | CUL7 | 15829 | -0.094 | 0.08 | NO |
225 | TRIM32 | TRIM32 | TRIM32 | 15872 | -0.095 | 0.082 | NO |
226 | GAN | GAN | GAN | 15908 | -0.096 | 0.085 | NO |
227 | ITGB5 | ITGB5 | ITGB5 | 16004 | -0.099 | 0.084 | NO |
228 | KLHL13 | KLHL13 | KLHL13 | 16187 | -0.1 | 0.079 | NO |
229 | SKP2 | SKP2 | SKP2 | 16363 | -0.11 | 0.075 | NO |
230 | TRIM9 | TRIM9 | TRIM9 | 17216 | -0.16 | 0.035 | NO |
231 | FBXO17 | FBXO17 | FBXO17 | 17786 | -0.22 | 0.014 | NO |
Figure S29. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG VALINE LEUCINE AND ISOLEUCINE DEGRADATION.

Figure S30. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG VALINE LEUCINE AND ISOLEUCINE DEGRADATION, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S16. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | FASLG | FASLG | FASLG | 81 | 0.9 | 0.057 | YES |
2 | PRKCQ | PRKCQ | PRKCQ | 89 | 0.89 | 0.12 | YES |
3 | TNF | TNF | TNF | 202 | 0.8 | 0.17 | YES |
4 | GZMB | GZMB | GZMB | 252 | 0.76 | 0.22 | YES |
5 | TNFSF10 | TNFSF10 | TNFSF10 | 537 | 0.6 | 0.24 | YES |
6 | DAPK2 | DAPK2 | DAPK2 | 716 | 0.53 | 0.27 | YES |
7 | CASP10 | CASP10 | CASP10 | 804 | 0.51 | 0.3 | YES |
8 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.31 | YES |
9 | MST4 | MST4 | MST4 | 1061 | 0.43 | 0.34 | YES |
10 | FAS | FAS | FAS | 1406 | 0.35 | 0.35 | YES |
11 | UNC5A | UNC5A | UNC5A | 1407 | 0.35 | 0.37 | YES |
12 | HIST1H1D | HIST1H1D | HIST1H1D | 1533 | 0.32 | 0.39 | YES |
13 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.41 | YES |
14 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.42 | YES |
15 | CFLAR | CFLAR | CFLAR | 2149 | 0.23 | 0.41 | YES |
16 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.42 | YES |
17 | BMX | BMX | BMX | 2161 | 0.23 | 0.44 | YES |
18 | SATB1 | SATB1 | SATB1 | 2282 | 0.21 | 0.45 | YES |
19 | CASP8 | CASP8 | CASP8 | 2329 | 0.21 | 0.46 | YES |
20 | CDH1 | CDH1 | CDH1 | 2378 | 0.2 | 0.47 | YES |
21 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.48 | YES |
22 | PMAIP1 | PMAIP1 | PMAIP1 | 2592 | 0.19 | 0.48 | YES |
23 | BCL2L11 | BCL2L11 | BCL2L11 | 2705 | 0.18 | 0.49 | YES |
24 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.5 | YES |
25 | HIST1H1B | HIST1H1B | HIST1H1B | 2730 | 0.17 | 0.51 | YES |
26 | BMF | BMF | BMF | 2882 | 0.16 | 0.52 | YES |
27 | HMGB2 | HMGB2 | HMGB2 | 3394 | 0.13 | 0.5 | NO |
28 | CASP7 | CASP7 | CASP7 | 3402 | 0.13 | 0.5 | NO |
29 | CASP3 | CASP3 | CASP3 | 3414 | 0.13 | 0.51 | NO |
30 | DAPK1 | DAPK1 | DAPK1 | 3688 | 0.12 | 0.51 | NO |
31 | BCL2 | BCL2 | BCL2 | 3711 | 0.11 | 0.51 | NO |
32 | TRADD | TRADD | TRADD | 3783 | 0.11 | 0.52 | NO |
33 | DCC | DCC | DCC | 4070 | 0.1 | 0.51 | NO |
34 | LMNB1 | LMNB1 | LMNB1 | 4686 | 0.078 | 0.48 | NO |
35 | BIRC2 | BIRC2 | BIRC2 | 4826 | 0.074 | 0.48 | NO |
36 | ROCK1 | ROCK1 | ROCK1 | 4855 | 0.074 | 0.48 | NO |
37 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.47 | NO |
38 | TJP2 | TJP2 | TJP2 | 5092 | 0.067 | 0.48 | NO |
39 | CASP6 | CASP6 | CASP6 | 5113 | 0.067 | 0.48 | NO |
40 | APAF1 | APAF1 | APAF1 | 5146 | 0.066 | 0.48 | NO |
41 | XIAP | XIAP | XIAP | 5363 | 0.061 | 0.47 | NO |
42 | PRKCD | PRKCD | PRKCD | 5526 | 0.058 | 0.47 | NO |
43 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.46 | NO |
44 | TRAF2 | TRAF2 | TRAF2 | 5740 | 0.053 | 0.46 | NO |
45 | DBNL | DBNL | DBNL | 5842 | 0.051 | 0.46 | NO |
46 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.46 | NO |
47 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.45 | NO |
48 | LOC647859 | LOC647859 | LOC647859 | 6240 | 0.043 | 0.45 | NO |
49 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.44 | NO |
50 | BCL2L1 | BCL2L1 | BCL2L1 | 6483 | 0.038 | 0.44 | NO |
51 | BID | BID | BID | 6581 | 0.036 | 0.44 | NO |
52 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.43 | NO |
53 | BAK1 | BAK1 | BAK1 | 6772 | 0.033 | 0.43 | NO |
54 | UNC5B | UNC5B | UNC5B | 6847 | 0.032 | 0.43 | NO |
55 | PAK2 | PAK2 | PAK2 | 6974 | 0.03 | 0.42 | NO |
56 | APPL1 | APPL1 | APPL1 | 7000 | 0.029 | 0.43 | NO |
57 | HIST1H1E | HIST1H1E | HIST1H1E | 7092 | 0.028 | 0.42 | NO |
58 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.41 | NO |
59 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.4 | NO |
60 | HMGB1 | HMGB1 | HMGB1 | 7593 | 0.021 | 0.4 | NO |
61 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.4 | NO |
62 | RIPK1 | RIPK1 | RIPK1 | 7620 | 0.021 | 0.4 | NO |
63 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.38 | NO |
64 | APC | APC | APC | 8330 | 0.012 | 0.36 | NO |
65 | DFFB | DFFB | DFFB | 8341 | 0.012 | 0.36 | NO |
66 | CASP9 | CASP9 | CASP9 | 8352 | 0.011 | 0.36 | NO |
67 | ARHGAP10 | ARHGAP10 | ARHGAP10 | 8372 | 0.011 | 0.36 | NO |
68 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.36 | NO |
69 | YWHAB | YWHAB | YWHAB | 8455 | 0.0098 | 0.36 | NO |
70 | OCLN | OCLN | OCLN | 8620 | 0.0079 | 0.35 | NO |
71 | NMT1 | NMT1 | NMT1 | 8643 | 0.0076 | 0.35 | NO |
72 | TJP1 | TJP1 | TJP1 | 8680 | 0.0072 | 0.35 | NO |
73 | FNTA | FNTA | FNTA | 8718 | 0.0066 | 0.35 | NO |
74 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.34 | NO |
75 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.34 | NO |
76 | TNFRSF1A | TNFRSF1A | TNFRSF1A | 8906 | 0.0042 | 0.34 | NO |
77 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.34 | NO |
78 | HIST1H1C | HIST1H1C | HIST1H1C | 9049 | 0.0025 | 0.33 | NO |
79 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.33 | NO |
80 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.32 | NO |
81 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.32 | NO |
82 | ACIN1 | ACIN1 | ACIN1 | 9349 | -0.0011 | 0.31 | NO |
83 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.31 | NO |
84 | PPP3R1 | PPP3R1 | PPP3R1 | 9489 | -0.0028 | 0.31 | NO |
85 | GSN | GSN | GSN | 9748 | -0.0052 | 0.29 | NO |
86 | ADD1 | ADD1 | ADD1 | 9982 | -0.0079 | 0.28 | NO |
87 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.28 | NO |
88 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.28 | NO |
89 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.28 | NO |
90 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.27 | NO |
91 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.27 | NO |
92 | KPNA1 | KPNA1 | KPNA1 | 10399 | -0.012 | 0.26 | NO |
93 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.25 | NO |
94 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.25 | NO |
95 | KPNB1 | KPNB1 | KPNB1 | 10689 | -0.015 | 0.25 | NO |
96 | PKP1 | PKP1 | PKP1 | 10746 | -0.016 | 0.25 | NO |
97 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.25 | NO |
98 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.24 | NO |
99 | SPTAN1 | SPTAN1 | SPTAN1 | 10840 | -0.017 | 0.24 | NO |
100 | PLEC | PLEC | PLEC | 10886 | -0.018 | 0.24 | NO |
101 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.24 | NO |
102 | TP53 | TP53 | TP53 | 10990 | -0.019 | 0.24 | NO |
103 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.23 | NO |
104 | DYNLL1 | DYNLL1 | DYNLL1 | 11227 | -0.021 | 0.23 | NO |
105 | DSG3 | DSG3 | DSG3 | 11252 | -0.021 | 0.23 | NO |
106 | BAX | BAX | BAX | 11301 | -0.022 | 0.23 | NO |
107 | BCAP31 | BCAP31 | BCAP31 | 11386 | -0.022 | 0.23 | NO |
108 | CYCS | CYCS | CYCS | 11678 | -0.026 | 0.21 | NO |
109 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.19 | NO |
110 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.19 | NO |
111 | DNM1L | DNM1L | DNM1L | 12131 | -0.031 | 0.19 | NO |
112 | DYNLL2 | DYNLL2 | DYNLL2 | 12282 | -0.033 | 0.19 | NO |
113 | AKT1 | AKT1 | AKT1 | 12293 | -0.033 | 0.19 | NO |
114 | TFDP1 | TFDP1 | TFDP1 | 12400 | -0.035 | 0.18 | NO |
115 | DSP | DSP | DSP | 12409 | -0.035 | 0.19 | NO |
116 | STK24 | STK24 | STK24 | 12554 | -0.036 | 0.18 | NO |
117 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.18 | NO |
118 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.18 | NO |
119 | BAD | BAD | BAD | 12804 | -0.039 | 0.18 | NO |
120 | CTNNB1 | CTNNB1 | CTNNB1 | 12967 | -0.042 | 0.17 | NO |
121 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.16 | NO |
122 | BBC3 | BBC3 | BBC3 | 13192 | -0.044 | 0.16 | NO |
123 | MAPK8 | MAPK8 | MAPK8 | 13228 | -0.045 | 0.16 | NO |
124 | DIABLO | DIABLO | DIABLO | 13235 | -0.045 | 0.17 | NO |
125 | VIM | VIM | VIM | 13410 | -0.047 | 0.16 | NO |
126 | FADD | FADD | FADD | 13534 | -0.049 | 0.16 | NO |
127 | E2F1 | E2F1 | E2F1 | 13667 | -0.051 | 0.15 | NO |
128 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.14 | NO |
129 | MAGED1 | MAGED1 | MAGED1 | 13892 | -0.054 | 0.15 | NO |
130 | DFFA | DFFA | DFFA | 13915 | -0.055 | 0.15 | NO |
131 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.15 | NO |
132 | DAPK3 | DAPK3 | DAPK3 | 14206 | -0.059 | 0.14 | NO |
133 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.13 | NO |
134 | MAPT | MAPT | MAPT | 15117 | -0.076 | 0.1 | NO |
135 | TNFRSF10B | TNFRSF10B | TNFRSF10B | 15395 | -0.082 | 0.091 | NO |
136 | LMNA | LMNA | LMNA | 15490 | -0.084 | 0.091 | NO |
137 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.093 | NO |
138 | PTK2 | PTK2 | PTK2 | 16021 | -0.1 | 0.074 | NO |
139 | DSG1 | DSG1 | DSG1 | 16110 | -0.1 | 0.077 | NO |
140 | H1F0 | H1F0 | H1F0 | 16347 | -0.11 | 0.071 | NO |
141 | GAS2 | GAS2 | GAS2 | 16975 | -0.14 | 0.046 | NO |
142 | DSG2 | DSG2 | DSG2 | 17639 | -0.2 | 0.022 | NO |
Figure S31. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG HISTIDINE METABOLISM.

Figure S32. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG HISTIDINE METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S17. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | NCF4 | NCF4 | NCF4 | 497 | 0.62 | 0.038 | YES |
2 | FCGR1A | FCGR1A | FCGR1A | 623 | 0.57 | 0.091 | YES |
3 | FCGR1B | FCGR1B | FCGR1B | 626 | 0.57 | 0.15 | YES |
4 | CYBB | CYBB | CYBB | 677 | 0.55 | 0.2 | YES |
5 | NCF2 | NCF2 | NCF2 | 728 | 0.53 | 0.26 | YES |
6 | MRC1 | MRC1 | MRC1 | 977 | 0.45 | 0.29 | YES |
7 | CTSS | CTSS | CTSS | 981 | 0.45 | 0.34 | YES |
8 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.38 | YES |
9 | HLA-F | HLA-F | HLA-F | 1235 | 0.39 | 0.41 | YES |
10 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.43 | YES |
11 | TAP1 | TAP1 | TAP1 | 1724 | 0.29 | 0.45 | YES |
12 | HLA-G | HLA-G | HLA-G | 1734 | 0.28 | 0.48 | YES |
13 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.51 | YES |
14 | HLA-B | HLA-B | HLA-B | 1796 | 0.27 | 0.53 | YES |
15 | CYBA | CYBA | CYBA | 1912 | 0.26 | 0.55 | YES |
16 | CD207 | CD207 | CD207 | 2057 | 0.24 | 0.57 | YES |
17 | B2M | B2M | B2M | 2091 | 0.24 | 0.59 | YES |
18 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.61 | YES |
19 | HLA-C | HLA-C | HLA-C | 2224 | 0.22 | 0.63 | YES |
20 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.64 | YES |
21 | LNPEP | LNPEP | LNPEP | 2448 | 0.2 | 0.66 | YES |
22 | TAP2 | TAP2 | TAP2 | 2571 | 0.19 | 0.68 | YES |
23 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.69 | YES |
24 | HLA-A | HLA-A | HLA-A | 2779 | 0.17 | 0.7 | YES |
25 | CD36 | CD36 | CD36 | 4096 | 0.099 | 0.64 | NO |
26 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.59 | NO |
27 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.56 | NO |
28 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.55 | NO |
29 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.54 | NO |
30 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.53 | NO |
31 | ITGAV | ITGAV | ITGAV | 6492 | 0.038 | 0.54 | NO |
32 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.53 | NO |
33 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.5 | NO |
34 | SEC61B | SEC61B | SEC61B | 7359 | 0.024 | 0.5 | NO |
35 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.49 | NO |
36 | MRC2 | MRC2 | MRC2 | 7572 | 0.021 | 0.49 | NO |
37 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.49 | NO |
38 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.47 | NO |
39 | PDIA3 | PDIA3 | PDIA3 | 8111 | 0.014 | 0.46 | NO |
40 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.45 | NO |
41 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.43 | NO |
42 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.42 | NO |
43 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.42 | NO |
44 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.41 | NO |
45 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.4 | NO |
46 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.4 | NO |
47 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.39 | NO |
48 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.36 | NO |
49 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.36 | NO |
50 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.36 | NO |
51 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.35 | NO |
52 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.35 | NO |
53 | CALR | CALR | CALR | 10431 | -0.012 | 0.34 | NO |
54 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.34 | NO |
55 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.34 | NO |
56 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.33 | NO |
57 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.33 | NO |
58 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.32 | NO |
59 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.32 | NO |
60 | SEC61A1 | SEC61A1 | SEC61A1 | 11538 | -0.024 | 0.3 | NO |
61 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.27 | NO |
62 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.27 | NO |
63 | SEC61G | SEC61G | SEC61G | 12071 | -0.03 | 0.28 | NO |
64 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.25 | NO |
65 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.25 | NO |
66 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.23 | NO |
67 | SEC61A2 | SEC61A2 | SEC61A2 | 13395 | -0.047 | 0.22 | NO |
68 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.2 | NO |
69 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.2 | NO |
70 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.18 | NO |
71 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.13 | NO |
72 | ITGB5 | ITGB5 | ITGB5 | 16004 | -0.099 | 0.11 | NO |
Figure S33. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG TYROSINE METABOLISM.

Figure S34. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG TYROSINE METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S18. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CARD11 | CARD11 | CARD11 | 131 | 0.84 | 0.15 | YES |
2 | PRKCB | PRKCB | PRKCB | 348 | 0.7 | 0.26 | YES |
3 | PSMB9 | PSMB9 | PSMB9 | 1030 | 0.44 | 0.31 | YES |
4 | PSMA8 | PSMA8 | PSMA8 | 1535 | 0.32 | 0.34 | YES |
5 | PSMB10 | PSMB10 | PSMB10 | 1738 | 0.28 | 0.38 | YES |
6 | NFKBIA | NFKBIA | NFKBIA | 2005 | 0.25 | 0.41 | YES |
7 | REL | REL | REL | 2106 | 0.23 | 0.45 | YES |
8 | PSMB8 | PSMB8 | PSMB8 | 2154 | 0.23 | 0.49 | YES |
9 | PSME2 | PSME2 | PSME2 | 2382 | 0.2 | 0.51 | YES |
10 | MALT1 | MALT1 | MALT1 | 2679 | 0.18 | 0.53 | YES |
11 | PSME1 | PSME1 | PSME1 | 2709 | 0.18 | 0.56 | YES |
12 | NFKBIE | NFKBIE | NFKBIE | 3335 | 0.13 | 0.55 | NO |
13 | IKBKG | IKBKG | IKBKG | 4881 | 0.073 | 0.48 | NO |
14 | BCL10 | BCL10 | BCL10 | 4930 | 0.072 | 0.49 | NO |
15 | PSMA3 | PSMA3 | PSMA3 | 5063 | 0.068 | 0.49 | NO |
16 | MAP3K7 | MAP3K7 | MAP3K7 | 5313 | 0.062 | 0.49 | NO |
17 | CHUK | CHUK | CHUK | 5614 | 0.056 | 0.48 | NO |
18 | IKBKB | IKBKB | IKBKB | 5656 | 0.055 | 0.49 | NO |
19 | PSMA4 | PSMA4 | PSMA4 | 5667 | 0.055 | 0.5 | NO |
20 | PSMA5 | PSMA5 | PSMA5 | 5959 | 0.049 | 0.49 | NO |
21 | NFKBIB | NFKBIB | NFKBIB | 6019 | 0.048 | 0.5 | NO |
22 | PSMB1 | PSMB1 | PSMB1 | 6232 | 0.043 | 0.49 | NO |
23 | PSMA6 | PSMA6 | PSMA6 | 6469 | 0.038 | 0.49 | NO |
24 | PSMB3 | PSMB3 | PSMB3 | 6696 | 0.034 | 0.48 | NO |
25 | PSMA2 | PSMA2 | PSMA2 | 7301 | 0.025 | 0.45 | NO |
26 | PSME4 | PSME4 | PSME4 | 7503 | 0.022 | 0.45 | NO |
27 | PSMC6 | PSMC6 | PSMC6 | 7619 | 0.021 | 0.44 | NO |
28 | CUL1 | CUL1 | CUL1 | 7889 | 0.017 | 0.43 | NO |
29 | PSMD10 | PSMD10 | PSMD10 | 7998 | 0.016 | 0.43 | NO |
30 | PSMC2 | PSMC2 | PSMC2 | 8373 | 0.011 | 0.41 | NO |
31 | PSMB6 | PSMB6 | PSMB6 | 8803 | 0.0055 | 0.39 | NO |
32 | PSMB2 | PSMB2 | PSMB2 | 8874 | 0.0046 | 0.38 | NO |
33 | PSMA1 | PSMA1 | PSMA1 | 8974 | 0.0034 | 0.38 | NO |
34 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.37 | NO |
35 | PSMD9 | PSMD9 | PSMD9 | 9245 | 0.0003 | 0.36 | NO |
36 | PSMC5 | PSMC5 | PSMC5 | 9267 | -0.000016 | 0.36 | NO |
37 | PSMF1 | PSMF1 | PSMF1 | 9446 | -0.0024 | 0.35 | NO |
38 | PSMD7 | PSMD7 | PSMD7 | 10012 | -0.0082 | 0.32 | NO |
39 | PSMD13 | PSMD13 | PSMD13 | 10104 | -0.0093 | 0.32 | NO |
40 | PSMD12 | PSMD12 | PSMD12 | 10107 | -0.0093 | 0.32 | NO |
41 | SKP1 | SKP1 | SKP1 | 10135 | -0.0096 | 0.32 | NO |
42 | PSMC1 | PSMC1 | PSMC1 | 10207 | -0.01 | 0.32 | NO |
43 | PSMD5 | PSMD5 | PSMD5 | 10249 | -0.011 | 0.32 | NO |
44 | PSMD14 | PSMD14 | PSMD14 | 10573 | -0.014 | 0.3 | NO |
45 | PSMA7 | PSMA7 | PSMA7 | 10642 | -0.015 | 0.3 | NO |
46 | PSMD6 | PSMD6 | PSMD6 | 10761 | -0.016 | 0.3 | NO |
47 | PSMD11 | PSMD11 | PSMD11 | 10815 | -0.017 | 0.3 | NO |
48 | RELA | RELA | RELA | 10903 | -0.018 | 0.3 | NO |
49 | PSMD1 | PSMD1 | PSMD1 | 10931 | -0.018 | 0.3 | NO |
50 | PSMC3 | PSMC3 | PSMC3 | 11140 | -0.02 | 0.29 | NO |
51 | BTRC | BTRC | BTRC | 11191 | -0.021 | 0.29 | NO |
52 | PSMC4 | PSMC4 | PSMC4 | 12038 | -0.03 | 0.25 | NO |
53 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.26 | NO |
54 | FBXW11 | FBXW11 | FBXW11 | 12300 | -0.033 | 0.25 | NO |
55 | PSMD3 | PSMD3 | PSMD3 | 12584 | -0.037 | 0.24 | NO |
56 | PSMD2 | PSMD2 | PSMD2 | 12752 | -0.039 | 0.24 | NO |
57 | PSMB4 | PSMB4 | PSMB4 | 13132 | -0.044 | 0.22 | NO |
58 | PSMD4 | PSMD4 | PSMD4 | 13869 | -0.054 | 0.19 | NO |
59 | PSMD8 | PSMD8 | PSMD8 | 14061 | -0.057 | 0.19 | NO |
60 | PSMB7 | PSMB7 | PSMB7 | 14548 | -0.065 | 0.18 | NO |
61 | PSMB5 | PSMB5 | PSMB5 | 15570 | -0.086 | 0.14 | NO |
Figure S35. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG TRYPTOPHAN METABOLISM.

Figure S36. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG TRYPTOPHAN METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S19. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | PRKCQ | PRKCQ | PRKCQ | 89 | 0.89 | 0.13 | YES |
2 | AKAP5 | AKAP5 | AKAP5 | 270 | 0.75 | 0.24 | YES |
3 | PRKCB | PRKCB | PRKCB | 348 | 0.7 | 0.35 | YES |
4 | MAP3K8 | MAP3K8 | MAP3K8 | 535 | 0.61 | 0.43 | YES |
5 | PRKCH | PRKCH | PRKCH | 1309 | 0.37 | 0.45 | YES |
6 | PIM1 | PIM1 | PIM1 | 1329 | 0.36 | 0.5 | YES |
7 | CAMK4 | CAMK4 | CAMK4 | 1414 | 0.34 | 0.55 | YES |
8 | NFATC2 | NFATC2 | NFATC2 | 1809 | 0.27 | 0.57 | YES |
9 | PRKCG | PRKCG | PRKCG | 2148 | 0.23 | 0.59 | YES |
10 | NFATC1 | NFATC1 | NFATC1 | 2625 | 0.18 | 0.59 | YES |
11 | CASP3 | CASP3 | CASP3 | 3414 | 0.13 | 0.57 | NO |
12 | BCL2 | BCL2 | BCL2 | 3711 | 0.11 | 0.57 | NO |
13 | PRKCZ | PRKCZ | PRKCZ | 3938 | 0.1 | 0.58 | NO |
14 | NFATC3 | NFATC3 | NFATC3 | 4829 | 0.074 | 0.54 | NO |
15 | MAP3K1 | MAP3K1 | MAP3K1 | 4925 | 0.072 | 0.54 | NO |
16 | PPP3CB | PPP3CB | PPP3CB | 5096 | 0.067 | 0.55 | NO |
17 | PRKCD | PRKCD | PRKCD | 5526 | 0.058 | 0.53 | NO |
18 | RCAN2 | RCAN2 | RCAN2 | 5568 | 0.057 | 0.54 | NO |
19 | BCL2L1 | BCL2L1 | BCL2L1 | 6483 | 0.038 | 0.49 | NO |
20 | PPP3CA | PPP3CA | PPP3CA | 6832 | 0.032 | 0.48 | NO |
21 | YWHAH | YWHAH | YWHAH | 6992 | 0.03 | 0.47 | NO |
22 | MAPK9 | MAPK9 | MAPK9 | 7793 | 0.019 | 0.43 | NO |
23 | YWHAB | YWHAB | YWHAB | 8455 | 0.0098 | 0.4 | NO |
24 | CSNK1A1 | CSNK1A1 | CSNK1A1 | 8555 | 0.0086 | 0.39 | NO |
25 | XPO1 | XPO1 | XPO1 | 9118 | 0.0016 | 0.36 | NO |
26 | MAPK14 | MAPK14 | MAPK14 | 9181 | 0.00082 | 0.36 | NO |
27 | PPP3R1 | PPP3R1 | PPP3R1 | 9489 | -0.0028 | 0.34 | NO |
28 | MAPK3 | MAPK3 | MAPK3 | 9630 | -0.0041 | 0.34 | NO |
29 | SFN | SFN | SFN | 9759 | -0.0053 | 0.33 | NO |
30 | RCAN1 | RCAN1 | RCAN1 | 9882 | -0.0067 | 0.32 | NO |
31 | FKBP1A | FKBP1A | FKBP1A | 10039 | -0.0085 | 0.32 | NO |
32 | YWHAZ | YWHAZ | YWHAZ | 10112 | -0.0094 | 0.31 | NO |
33 | CREBBP | CREBBP | CREBBP | 10160 | -0.0098 | 0.31 | NO |
34 | CABIN1 | CABIN1 | CABIN1 | 10550 | -0.014 | 0.29 | NO |
35 | KPNB1 | KPNB1 | KPNB1 | 10689 | -0.015 | 0.29 | NO |
36 | EP300 | EP300 | EP300 | 10906 | -0.018 | 0.28 | NO |
37 | GSK3B | GSK3B | GSK3B | 11111 | -0.02 | 0.27 | NO |
38 | BAX | BAX | BAX | 11301 | -0.022 | 0.26 | NO |
39 | FKBP8 | FKBP8 | FKBP8 | 11403 | -0.023 | 0.26 | NO |
40 | KPNA2 | KPNA2 | KPNA2 | 11406 | -0.023 | 0.26 | NO |
41 | MEF2D | MEF2D | MEF2D | 11419 | -0.023 | 0.27 | NO |
42 | PRKACA | PRKACA | PRKACA | 11544 | -0.024 | 0.26 | NO |
43 | RAN | RAN | RAN | 11817 | -0.028 | 0.25 | NO |
44 | CHP | CHP | CHP | 11876 | -0.028 | 0.26 | NO |
45 | BAD | BAD | BAD | 12804 | -0.039 | 0.21 | NO |
46 | NUP214 | NUP214 | NUP214 | 13016 | -0.042 | 0.2 | NO |
47 | YWHAG | YWHAG | YWHAG | 13191 | -0.044 | 0.2 | NO |
48 | MAPK8 | MAPK8 | MAPK8 | 13228 | -0.045 | 0.21 | NO |
49 | YWHAQ | YWHAQ | YWHAQ | 13295 | -0.046 | 0.21 | NO |
50 | YWHAE | YWHAE | YWHAE | 13356 | -0.046 | 0.21 | NO |
51 | PRKCE | PRKCE | PRKCE | 14440 | -0.063 | 0.16 | NO |
52 | NR4A1 | NR4A1 | NR4A1 | 14797 | -0.07 | 0.16 | NO |
53 | CSNK2A1 | CSNK2A1 | CSNK2A1 | 14812 | -0.07 | 0.16 | NO |
54 | PRKCA | PRKCA | PRKCA | 15766 | -0.092 | 0.13 | NO |
Figure S37. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG O GLYCAN BIOSYNTHESIS.
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Figure S38. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG O GLYCAN BIOSYNTHESIS, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S20. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | ZBP1 | ZBP1 | ZBP1 | 47 | 0.94 | 0.13 | YES |
2 | NOD2 | NOD2 | NOD2 | 442 | 0.65 | 0.2 | YES |
3 | RIPK3 | RIPK3 | RIPK3 | 501 | 0.62 | 0.28 | YES |
4 | IRAK2 | IRAK2 | IRAK2 | 1268 | 0.38 | 0.29 | YES |
5 | IRF7 | IRF7 | IRF7 | 1845 | 0.27 | 0.29 | YES |
6 | TLR3 | TLR3 | TLR3 | 1873 | 0.26 | 0.33 | YES |
7 | IKBKE | IKBKE | IKBKE | 1929 | 0.26 | 0.36 | YES |
8 | NFKBIA | NFKBIA | NFKBIA | 2005 | 0.25 | 0.39 | YES |
9 | AGER | AGER | AGER | 2158 | 0.23 | 0.41 | YES |
10 | S100A12 | S100A12 | S100A12 | 2256 | 0.22 | 0.44 | YES |
11 | NFKB2 | NFKB2 | NFKB2 | 2379 | 0.2 | 0.46 | YES |
12 | RPS6KA1 | RPS6KA1 | RPS6KA1 | 2420 | 0.2 | 0.48 | YES |
13 | MAP2K6 | MAP2K6 | MAP2K6 | 3174 | 0.14 | 0.46 | YES |
14 | S100B | S100B | S100B | 3334 | 0.13 | 0.47 | YES |
15 | MEF2C | MEF2C | MEF2C | 3416 | 0.13 | 0.48 | YES |
16 | RPS6KA2 | RPS6KA2 | RPS6KA2 | 3428 | 0.13 | 0.5 | YES |
17 | SAA1 | SAA1 | SAA1 | 3853 | 0.11 | 0.49 | YES |
18 | NOD1 | NOD1 | NOD1 | 4068 | 0.1 | 0.49 | YES |
19 | TAB2 | TAB2 | TAB2 | 4093 | 0.099 | 0.51 | YES |
20 | TBK1 | TBK1 | TBK1 | 4385 | 0.088 | 0.5 | YES |
21 | RIPK2 | RIPK2 | RIPK2 | 4414 | 0.087 | 0.51 | YES |
22 | IRF3 | IRF3 | IRF3 | 4573 | 0.082 | 0.52 | YES |
23 | IKBKG | IKBKG | IKBKG | 4881 | 0.073 | 0.51 | YES |
24 | TAB3 | TAB3 | TAB3 | 5244 | 0.064 | 0.5 | YES |
25 | MAP3K7 | MAP3K7 | MAP3K7 | 5313 | 0.062 | 0.5 | YES |
26 | CREB1 | CREB1 | CREB1 | 5420 | 0.06 | 0.5 | YES |
27 | RPS6KA5 | RPS6KA5 | RPS6KA5 | 5445 | 0.06 | 0.51 | YES |
28 | MAPK10 | MAPK10 | MAPK10 | 5471 | 0.059 | 0.52 | YES |
29 | CHUK | CHUK | CHUK | 5614 | 0.056 | 0.52 | YES |
30 | IKBKB | IKBKB | IKBKB | 5656 | 0.055 | 0.52 | YES |
31 | MAP2K3 | MAP2K3 | MAP2K3 | 5962 | 0.049 | 0.51 | YES |
32 | ATF1 | ATF1 | ATF1 | 5994 | 0.048 | 0.52 | YES |
33 | NFKBIB | NFKBIB | NFKBIB | 6019 | 0.048 | 0.52 | YES |
34 | MEF2A | MEF2A | MEF2A | 6059 | 0.046 | 0.53 | YES |
35 | MAP2K1 | MAP2K1 | MAP2K1 | 6451 | 0.039 | 0.51 | NO |
36 | FOS | FOS | FOS | 6514 | 0.038 | 0.51 | NO |
37 | MAPKAPK2 | MAPKAPK2 | MAPKAPK2 | 6552 | 0.037 | 0.52 | NO |
38 | PPP2CA | PPP2CA | PPP2CA | 6793 | 0.033 | 0.51 | NO |
39 | RPS6KA3 | RPS6KA3 | RPS6KA3 | 7071 | 0.028 | 0.5 | NO |
40 | MAP2K4 | MAP2K4 | MAP2K4 | 7210 | 0.026 | 0.49 | NO |
41 | PPP2R1B | PPP2R1B | PPP2R1B | 7487 | 0.022 | 0.48 | NO |
42 | HMGB1 | HMGB1 | HMGB1 | 7593 | 0.021 | 0.48 | NO |
43 | RIPK1 | RIPK1 | RIPK1 | 7620 | 0.021 | 0.48 | NO |
44 | MAPK11 | MAPK11 | MAPK11 | 7707 | 0.02 | 0.48 | NO |
45 | MAPK9 | MAPK9 | MAPK9 | 7793 | 0.019 | 0.47 | NO |
46 | JUN | JUN | JUN | 7979 | 0.016 | 0.46 | NO |
47 | TICAM1 | TICAM1 | TICAM1 | 8297 | 0.012 | 0.45 | NO |
48 | RPS27A | RPS27A | RPS27A | 9105 | 0.0018 | 0.4 | NO |
49 | ATF2 | ATF2 | ATF2 | 9155 | 0.0012 | 0.4 | NO |
50 | MAPK14 | MAPK14 | MAPK14 | 9181 | 0.00082 | 0.4 | NO |
51 | MAPKAPK3 | MAPKAPK3 | MAPKAPK3 | 9409 | -0.0019 | 0.39 | NO |
52 | MAPK3 | MAPK3 | MAPK3 | 9630 | -0.0041 | 0.38 | NO |
53 | DUSP6 | DUSP6 | DUSP6 | 9633 | -0.0042 | 0.38 | NO |
54 | IRAK1 | IRAK1 | IRAK1 | 10506 | -0.013 | 0.33 | NO |
55 | TRAF6 | TRAF6 | TRAF6 | 10774 | -0.016 | 0.32 | NO |
56 | DUSP3 | DUSP3 | DUSP3 | 10816 | -0.017 | 0.32 | NO |
57 | RELA | RELA | RELA | 10903 | -0.018 | 0.32 | NO |
58 | CDK1 | CDK1 | CDK1 | 11248 | -0.021 | 0.3 | NO |
59 | ELK1 | ELK1 | ELK1 | 11556 | -0.025 | 0.29 | NO |
60 | MAP2K2 | MAP2K2 | MAP2K2 | 11676 | -0.026 | 0.28 | NO |
61 | UBA52 | UBA52 | UBA52 | 12069 | -0.03 | 0.26 | NO |
62 | APP | APP | APP | 12137 | -0.031 | 0.27 | NO |
63 | TAB1 | TAB1 | TAB1 | 12299 | -0.033 | 0.26 | NO |
64 | MAPK1 | MAPK1 | MAPK1 | 12399 | -0.035 | 0.26 | NO |
65 | MAPK7 | MAPK7 | MAPK7 | 12616 | -0.037 | 0.25 | NO |
66 | PPP2CB | PPP2CB | PPP2CB | 12722 | -0.038 | 0.25 | NO |
67 | MAPK8 | MAPK8 | MAPK8 | 13228 | -0.045 | 0.23 | NO |
68 | DUSP7 | DUSP7 | DUSP7 | 13658 | -0.051 | 0.21 | NO |
69 | PPP2R5D | PPP2R5D | PPP2R5D | 13740 | -0.052 | 0.22 | NO |
70 | DUSP4 | DUSP4 | DUSP4 | 13758 | -0.052 | 0.22 | NO |
71 | MAP2K7 | MAP2K7 | MAP2K7 | 13805 | -0.053 | 0.23 | NO |
72 | PPP2R1A | PPP2R1A | PPP2R1A | 13943 | -0.055 | 0.23 | NO |
Figure S39. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM.

Figure S40. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

For the top enriched genes, if you want to check whether they are
-
up-regulated, please check the list of up-regulated genes
-
down-regulated, please check the list of down-regulated genes
For the top enriched genes, if you want to check whether they are
-
highly expressed genes, please check the list of high (top 30%) expressed genes
-
low expressed genes, please check the list of low (bottom 30%) expressed genes
An expression pattern of top(30%)/middle(30%)/low(30%) in this subtype against other subtypes is available in a heatmap
For the top enriched genes, if you want to check whether they are
-
significantly differently expressed genes by eBayes lm fit, please check the list of significant genes
Table 5. Get Full Table This table shows top 10 pathways which are significantly enriched in cluster clus3. It displays only significant gene sets satisfying nom.p.val.threshold (-1), fwer.p.val.threshold (-1) , fdr.q.val.threshold (0.25) and the default table is sorted by Normalized Enrichment Score (NES). Further details on NES statistics, please visit The Broad GSEA website.
GeneSet(GS) | Size(#genes) | genes.ES.table | ES | NES | NOM.p.val | FDR.q.val | FWER.p.val | Tag.. | Gene.. | Signal | FDR..median. | glob.p.val |
---|---|---|---|---|---|---|---|---|---|---|---|---|
KEGG TGF BETA SIGNALING PATHWAY | 85 | genes.ES.table | 0.56 | 1.7 | 0.004 | 0.82 | 0.84 | 0.4 | 0.21 | 0.32 | 0.36 | 0.23 |
KEGG ECM RECEPTOR INTERACTION | 82 | genes.ES.table | 0.62 | 1.6 | 0.0079 | 0.62 | 0.91 | 0.66 | 0.22 | 0.52 | 0.33 | 0.17 |
BIOCARTA ERK PATHWAY | 28 | genes.ES.table | 0.49 | 1.8 | 0.0097 | 1 | 0.63 | 0.18 | 0.094 | 0.16 | 0.3 | 0.24 |
BIOCARTA MPR PATHWAY | 33 | genes.ES.table | 0.48 | 1.6 | 0.025 | 0.68 | 0.9 | 0.21 | 0.18 | 0.17 | 0.35 | 0.19 |
WNT SIGNALING | 84 | genes.ES.table | 0.5 | 1.7 | 0.0039 | 0.69 | 0.9 | 0.29 | 0.16 | 0.24 | 0.35 | 0.19 |
ST INTEGRIN SIGNALING PATHWAY | 80 | genes.ES.table | 0.43 | 1.6 | 0.017 | 0.64 | 0.9 | 0.38 | 0.26 | 0.28 | 0.33 | 0.18 |
PID INTEGRIN1 PATHWAY | 64 | genes.ES.table | 0.68 | 1.7 | 0.0057 | 0.8 | 0.87 | 0.7 | 0.2 | 0.56 | 0.38 | 0.22 |
PID EPHBFWDPATHWAY | 40 | genes.ES.table | 0.53 | 1.7 | 0.0061 | 0.83 | 0.79 | 0.52 | 0.33 | 0.35 | 0.31 | 0.22 |
PID RET PATHWAY | 39 | genes.ES.table | 0.51 | 1.7 | 0.016 | 0.92 | 0.77 | 0.46 | 0.33 | 0.31 | 0.34 | 0.24 |
PID NCADHERINPATHWAY | 33 | genes.ES.table | 0.59 | 1.9 | 0 | 1 | 0.46 | 0.36 | 0.25 | 0.27 | 0 | 0.38 |
Table S21. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | GRIA2 | GRIA2 | GRIA2 | 14 | 0.7 | 0.16 | YES |
2 | GAP43 | GAP43 | GAP43 | 20 | 0.68 | 0.31 | YES |
3 | CDH2 | CDH2 | CDH2 | 181 | 0.5 | 0.42 | YES |
4 | FGFR1 | FGFR1 | FGFR1 | 931 | 0.33 | 0.45 | YES |
5 | GJA1 | GJA1 | GJA1 | 957 | 0.33 | 0.52 | YES |
6 | CNR1 | CNR1 | CNR1 | 2254 | 0.22 | 0.5 | YES |
7 | MAPK8 | MAPK8 | MAPK8 | 2521 | 0.2 | 0.53 | YES |
8 | PIK3CA | PIK3CA | PIK3CA | 3182 | 0.17 | 0.54 | YES |
9 | DAGLA | DAGLA | DAGLA | 3489 | 0.16 | 0.55 | YES |
10 | LRP5 | LRP5 | LRP5 | 4264 | 0.13 | 0.54 | YES |
11 | ROCK1 | ROCK1 | ROCK1 | 4469 | 0.12 | 0.56 | YES |
12 | KIF5B | KIF5B | KIF5B | 4488 | 0.12 | 0.58 | YES |
13 | FER | FER | FER | 5831 | 0.092 | 0.53 | NO |
14 | GSN | GSN | GSN | 6611 | 0.075 | 0.5 | NO |
15 | PTPN11 | PTPN11 | PTPN11 | 7059 | 0.066 | 0.5 | NO |
16 | CAMK2G | CAMK2G | CAMK2G | 8760 | 0.035 | 0.41 | NO |
17 | CTNNA1 | CTNNA1 | CTNNA1 | 8802 | 0.034 | 0.41 | NO |
18 | PIK3R1 | PIK3R1 | PIK3R1 | 9562 | 0.021 | 0.38 | NO |
19 | RAC1 | RAC1 | RAC1 | 9686 | 0.02 | 0.38 | NO |
20 | CDC42 | CDC42 | CDC42 | 10252 | 0.011 | 0.35 | NO |
21 | PIP5K1C | PIP5K1C | PIP5K1C | 10676 | 0.0043 | 0.32 | NO |
22 | DAGLB | DAGLB | DAGLB | 11100 | -0.0023 | 0.3 | NO |
23 | RHOA | RHOA | RHOA | 11198 | -0.004 | 0.3 | NO |
24 | MAPRE1 | MAPRE1 | MAPRE1 | 11277 | -0.0054 | 0.29 | NO |
25 | PLCG1 | PLCG1 | PLCG1 | 11531 | -0.0092 | 0.28 | NO |
26 | DCTN1 | DCTN1 | DCTN1 | 11668 | -0.011 | 0.28 | NO |
27 | JUP | JUP | JUP | 12824 | -0.029 | 0.22 | NO |
28 | PTPN1 | PTPN1 | PTPN1 | 12853 | -0.03 | 0.22 | NO |
29 | CTTN | CTTN | CTTN | 12954 | -0.031 | 0.22 | NO |
30 | CTNND1 | CTNND1 | CTNND1 | 13053 | -0.033 | 0.23 | NO |
31 | AXIN1 | AXIN1 | AXIN1 | 13659 | -0.043 | 0.2 | NO |
32 | CTNNB1 | CTNNB1 | CTNNB1 | 15295 | -0.081 | 0.13 | NO |
33 | MYL2 | MYL2 | MYL2 | 15760 | -0.097 | 0.13 | NO |
Figure S41. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG TGF BETA SIGNALING PATHWAY.

Figure S42. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG TGF BETA SIGNALING PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S22. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CHST1 | CHST1 | CHST1 | 270 | 0.46 | 0.077 | YES |
2 | ACAN | ACAN | ACAN | 707 | 0.37 | 0.13 | YES |
3 | B4GALT6 | B4GALT6 | B4GALT6 | 789 | 0.35 | 0.19 | YES |
4 | CHST6 | CHST6 | CHST6 | 1131 | 0.31 | 0.23 | YES |
5 | CHST5 | CHST5 | CHST5 | 1242 | 0.3 | 0.29 | YES |
6 | CHST2 | CHST2 | CHST2 | 1334 | 0.29 | 0.34 | YES |
7 | OGN | OGN | OGN | 1352 | 0.29 | 0.4 | YES |
8 | LUM | LUM | LUM | 1465 | 0.28 | 0.44 | YES |
9 | FMOD | FMOD | FMOD | 1478 | 0.28 | 0.5 | YES |
10 | KERA | KERA | KERA | 1486 | 0.28 | 0.55 | YES |
11 | B3GNT7 | B3GNT7 | B3GNT7 | 1537 | 0.27 | 0.6 | YES |
12 | OMD | OMD | OMD | 1833 | 0.25 | 0.64 | YES |
13 | B3GNT3 | B3GNT3 | B3GNT3 | 1998 | 0.24 | 0.67 | YES |
14 | B3GNT2 | B3GNT2 | B3GNT2 | 6582 | 0.075 | 0.43 | NO |
15 | B4GALT4 | B4GALT4 | B4GALT4 | 6722 | 0.072 | 0.44 | NO |
16 | ST3GAL2 | ST3GAL2 | ST3GAL2 | 7365 | 0.06 | 0.42 | NO |
17 | B4GALT5 | B4GALT5 | B4GALT5 | 8346 | 0.042 | 0.37 | NO |
18 | GNS | GNS | GNS | 8427 | 0.04 | 0.37 | NO |
19 | B4GALT2 | B4GALT2 | B4GALT2 | 9083 | 0.029 | 0.34 | NO |
20 | ST3GAL1 | ST3GAL1 | ST3GAL1 | 9868 | 0.016 | 0.3 | NO |
21 | HEXB | HEXB | HEXB | 10555 | 0.0061 | 0.27 | NO |
22 | B4GALT1 | B4GALT1 | B4GALT1 | 11053 | -0.0015 | 0.24 | NO |
23 | B3GNT1 | B3GNT1 | B3GNT1 | 11935 | -0.015 | 0.19 | NO |
24 | ST3GAL3 | ST3GAL3 | ST3GAL3 | 12461 | -0.023 | 0.17 | NO |
25 | B4GALT3 | B4GALT3 | B4GALT3 | 13250 | -0.036 | 0.13 | NO |
26 | B3GNT4 | B3GNT4 | B3GNT4 | 14030 | -0.051 | 0.098 | NO |
27 | GLB1 | GLB1 | GLB1 | 14524 | -0.061 | 0.083 | NO |
28 | HEXA | HEXA | HEXA | 16319 | -0.12 | 0.0079 | NO |
29 | PRELP | PRELP | PRELP | 17371 | -0.22 | -0.0068 | NO |
30 | ST3GAL6 | ST3GAL6 | ST3GAL6 | 17376 | -0.22 | 0.037 | NO |
Figure S43. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG ECM RECEPTOR INTERACTION.

Figure S44. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG ECM RECEPTOR INTERACTION, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S23. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CHST1 | CHST1 | CHST1 | 270 | 0.46 | 0.082 | YES |
2 | ACAN | ACAN | ACAN | 707 | 0.37 | 0.13 | YES |
3 | B4GALT6 | B4GALT6 | B4GALT6 | 789 | 0.35 | 0.2 | YES |
4 | CHST6 | CHST6 | CHST6 | 1131 | 0.31 | 0.25 | YES |
5 | CHST5 | CHST5 | CHST5 | 1242 | 0.3 | 0.3 | YES |
6 | CHST2 | CHST2 | CHST2 | 1334 | 0.29 | 0.36 | YES |
7 | OGN | OGN | OGN | 1352 | 0.29 | 0.42 | YES |
8 | LUM | LUM | LUM | 1465 | 0.28 | 0.47 | YES |
9 | FMOD | FMOD | FMOD | 1478 | 0.28 | 0.52 | YES |
10 | KERA | KERA | KERA | 1486 | 0.28 | 0.58 | YES |
11 | B3GNT7 | B3GNT7 | B3GNT7 | 1537 | 0.27 | 0.64 | YES |
12 | OMD | OMD | OMD | 1833 | 0.25 | 0.67 | YES |
13 | B3GNT3 | B3GNT3 | B3GNT3 | 1998 | 0.24 | 0.71 | YES |
14 | B3GNT2 | B3GNT2 | B3GNT2 | 6582 | 0.075 | 0.47 | NO |
15 | B4GALT4 | B4GALT4 | B4GALT4 | 6722 | 0.072 | 0.48 | NO |
16 | ST3GAL2 | ST3GAL2 | ST3GAL2 | 7365 | 0.06 | 0.46 | NO |
17 | B4GALT5 | B4GALT5 | B4GALT5 | 8346 | 0.042 | 0.41 | NO |
18 | B4GALT2 | B4GALT2 | B4GALT2 | 9083 | 0.029 | 0.38 | NO |
19 | ST3GAL1 | ST3GAL1 | ST3GAL1 | 9868 | 0.016 | 0.34 | NO |
20 | B4GALT1 | B4GALT1 | B4GALT1 | 11053 | -0.0015 | 0.27 | NO |
21 | B3GNT1 | B3GNT1 | B3GNT1 | 11935 | -0.015 | 0.22 | NO |
22 | ST3GAL3 | ST3GAL3 | ST3GAL3 | 12461 | -0.023 | 0.2 | NO |
23 | B4GALT3 | B4GALT3 | B4GALT3 | 13250 | -0.036 | 0.16 | NO |
24 | B3GNT4 | B3GNT4 | B3GNT4 | 14030 | -0.051 | 0.13 | NO |
25 | PRELP | PRELP | PRELP | 17371 | -0.22 | -0.0089 | NO |
26 | ST3GAL6 | ST3GAL6 | ST3GAL6 | 17376 | -0.22 | 0.037 | NO |
Figure S45. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: BIOCARTA ERK PATHWAY.

Figure S46. Get High-res Image For the top 5 core enriched genes in the pathway: BIOCARTA ERK PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S24. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | NGFR | NGFR | NGFR | 207 | 0.49 | 0.15 | YES |
2 | PDGFRA | PDGFRA | PDGFRA | 499 | 0.4 | 0.27 | YES |
3 | EGFR | EGFR | EGFR | 1209 | 0.3 | 0.33 | YES |
4 | NGF | NGF | NGF | 1313 | 0.29 | 0.43 | YES |
5 | GNGT1 | GNGT1 | GNGT1 | 1688 | 0.26 | 0.49 | YES |
6 | ITGB1 | ITGB1 | ITGB1 | 3599 | 0.15 | 0.44 | NO |
7 | SHC1 | SHC1 | SHC1 | 4369 | 0.13 | 0.44 | NO |
8 | MAP2K1 | MAP2K1 | MAP2K1 | 5996 | 0.088 | 0.38 | NO |
9 | GNAS | GNAS | GNAS | 6541 | 0.076 | 0.37 | NO |
10 | PTPRR | PTPRR | PTPRR | 7586 | 0.056 | 0.33 | NO |
11 | STAT3 | STAT3 | STAT3 | 8278 | 0.043 | 0.31 | NO |
12 | SOS1 | SOS1 | SOS1 | 10079 | 0.013 | 0.22 | NO |
13 | IGF1R | IGF1R | IGF1R | 10080 | 0.013 | 0.22 | NO |
14 | RAF1 | RAF1 | RAF1 | 10243 | 0.011 | 0.21 | NO |
15 | HRAS | HRAS | HRAS | 10631 | 0.0049 | 0.19 | NO |
16 | GNB1 | GNB1 | GNB1 | 10645 | 0.0047 | 0.2 | NO |
17 | PPP2CA | PPP2CA | PPP2CA | 10702 | 0.0039 | 0.19 | NO |
18 | ELK1 | ELK1 | ELK1 | 10766 | 0.0029 | 0.19 | NO |
19 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.15 | NO |
20 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.12 | NO |
21 | RPS6KA1 | RPS6KA1 | RPS6KA1 | 12903 | -0.03 | 0.092 | NO |
22 | GRB2 | GRB2 | GRB2 | 12936 | -0.031 | 0.1 | NO |
23 | MKNK2 | MKNK2 | MKNK2 | 13335 | -0.037 | 0.091 | NO |
24 | MKNK1 | MKNK1 | MKNK1 | 13684 | -0.044 | 0.086 | NO |
25 | SRC | SRC | SRC | 14012 | -0.051 | 0.085 | NO |
26 | MAP2K2 | MAP2K2 | MAP2K2 | 14101 | -0.052 | 0.098 | NO |
27 | MYC | MYC | MYC | 16753 | -0.15 | 0.0025 | NO |
28 | RPS6KA5 | RPS6KA5 | RPS6KA5 | 17276 | -0.2 | 0.042 | NO |
Figure S47. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: BIOCARTA MPR PATHWAY.

Figure S48. Get High-res Image For the top 5 core enriched genes in the pathway: BIOCARTA MPR PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S25. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | FGF2 | FGF2 | FGF2 | 55 | 0.61 | 0.14 | YES |
2 | FGF5 | FGF5 | FGF5 | 350 | 0.44 | 0.22 | YES |
3 | FGF10 | FGF10 | FGF10 | 600 | 0.38 | 0.29 | YES |
4 | FGF7 | FGF7 | FGF7 | 606 | 0.38 | 0.38 | YES |
5 | KL | KL | KL | 823 | 0.35 | 0.45 | YES |
6 | FGFR1 | FGFR1 | FGFR1 | 931 | 0.33 | 0.52 | YES |
7 | FGF18 | FGF18 | FGF18 | 1367 | 0.29 | 0.56 | YES |
8 | SPRY2 | SPRY2 | SPRY2 | 1613 | 0.26 | 0.6 | YES |
9 | FGF1 | FGF1 | FGF1 | 2376 | 0.21 | 0.61 | YES |
10 | FGF23 | FGF23 | FGF23 | 2779 | 0.19 | 0.63 | YES |
11 | KLB | KLB | KLB | 4457 | 0.13 | 0.57 | NO |
12 | BRAF | BRAF | BRAF | 5208 | 0.11 | 0.55 | NO |
13 | CBL | CBL | CBL | 5223 | 0.11 | 0.57 | NO |
14 | FGF8 | FGF8 | FGF8 | 6207 | 0.083 | 0.54 | NO |
15 | FRS2 | FRS2 | FRS2 | 6692 | 0.073 | 0.53 | NO |
16 | FGF22 | FGF22 | FGF22 | 7755 | 0.052 | 0.48 | NO |
17 | FGF9 | FGF9 | FGF9 | 8025 | 0.048 | 0.48 | NO |
18 | PPP2CB | PPP2CB | PPP2CB | 8310 | 0.042 | 0.47 | NO |
19 | FGF17 | FGF17 | FGF17 | 9436 | 0.023 | 0.41 | NO |
20 | RPS27A | RPS27A | RPS27A | 9842 | 0.017 | 0.4 | NO |
21 | FGFR2 | FGFR2 | FGFR2 | 10151 | 0.012 | 0.38 | NO |
22 | FGFR3 | FGFR3 | FGFR3 | 10526 | 0.0065 | 0.36 | NO |
23 | PPP2CA | PPP2CA | PPP2CA | 10702 | 0.0039 | 0.35 | NO |
24 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.31 | NO |
25 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.28 | NO |
26 | FGFR4 | FGFR4 | FGFR4 | 12659 | -0.027 | 0.26 | NO |
27 | PPP2R1A | PPP2R1A | PPP2R1A | 12767 | -0.028 | 0.26 | NO |
28 | UBA52 | UBA52 | UBA52 | 12928 | -0.031 | 0.26 | NO |
29 | GRB2 | GRB2 | GRB2 | 12936 | -0.031 | 0.26 | NO |
30 | MKNK1 | MKNK1 | MKNK1 | 13684 | -0.044 | 0.23 | NO |
31 | SRC | SRC | SRC | 14012 | -0.051 | 0.22 | NO |
Figure S49. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: WNT SIGNALING.

Figure S50. Get High-res Image For the top 5 core enriched genes in the pathway: WNT SIGNALING, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S26. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | FGF2 | FGF2 | FGF2 | 55 | 0.61 | 0.15 | YES |
2 | FGF5 | FGF5 | FGF5 | 350 | 0.44 | 0.24 | YES |
3 | FGF10 | FGF10 | FGF10 | 600 | 0.38 | 0.32 | YES |
4 | FGF7 | FGF7 | FGF7 | 606 | 0.38 | 0.41 | YES |
5 | KL | KL | KL | 823 | 0.35 | 0.49 | YES |
6 | FGFR1 | FGFR1 | FGFR1 | 931 | 0.33 | 0.56 | YES |
7 | FGF18 | FGF18 | FGF18 | 1367 | 0.29 | 0.61 | YES |
8 | FGF1 | FGF1 | FGF1 | 2376 | 0.21 | 0.61 | YES |
9 | FGF23 | FGF23 | FGF23 | 2779 | 0.19 | 0.63 | YES |
10 | KLB | KLB | KLB | 4457 | 0.13 | 0.57 | NO |
11 | KRAS | KRAS | KRAS | 5673 | 0.095 | 0.52 | NO |
12 | MAP2K1 | MAP2K1 | MAP2K1 | 5996 | 0.088 | 0.53 | NO |
13 | FGF8 | FGF8 | FGF8 | 6207 | 0.083 | 0.54 | NO |
14 | FRS2 | FRS2 | FRS2 | 6692 | 0.073 | 0.53 | NO |
15 | NRAS | NRAS | NRAS | 7693 | 0.054 | 0.48 | NO |
16 | FGF22 | FGF22 | FGF22 | 7755 | 0.052 | 0.5 | NO |
17 | FGF9 | FGF9 | FGF9 | 8025 | 0.048 | 0.49 | NO |
18 | FRS3 | FRS3 | FRS3 | 9412 | 0.024 | 0.42 | NO |
19 | FGF17 | FGF17 | FGF17 | 9436 | 0.023 | 0.42 | NO |
20 | SOS1 | SOS1 | SOS1 | 10079 | 0.013 | 0.39 | NO |
21 | FGFR2 | FGFR2 | FGFR2 | 10151 | 0.012 | 0.39 | NO |
22 | RAF1 | RAF1 | RAF1 | 10243 | 0.011 | 0.39 | NO |
23 | FGFR3 | FGFR3 | FGFR3 | 10526 | 0.0065 | 0.38 | NO |
24 | HRAS | HRAS | HRAS | 10631 | 0.0049 | 0.37 | NO |
25 | CDK1 | CDK1 | CDK1 | 11401 | -0.0072 | 0.33 | NO |
26 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.33 | NO |
27 | YWHAB | YWHAB | YWHAB | 12186 | -0.019 | 0.29 | NO |
28 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.3 | NO |
29 | FGFR4 | FGFR4 | FGFR4 | 12659 | -0.027 | 0.28 | NO |
30 | GRB2 | GRB2 | GRB2 | 12936 | -0.031 | 0.27 | NO |
31 | MAP2K2 | MAP2K2 | MAP2K2 | 14101 | -0.052 | 0.22 | NO |
Figure S51. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: ST INTEGRIN SIGNALING PATHWAY.

Figure S52. Get High-res Image For the top 5 core enriched genes in the pathway: ST INTEGRIN SIGNALING PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S27. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | GDNF | GDNF | GDNF | 38 | 0.65 | 0.15 | YES |
2 | GFRA1 | GFRA1 | GFRA1 | 652 | 0.37 | 0.2 | YES |
3 | PRKCA | PRKCA | PRKCA | 1326 | 0.29 | 0.23 | YES |
4 | DOK6 | DOK6 | DOK6 | 2042 | 0.23 | 0.25 | YES |
5 | RASA1 | RASA1 | RASA1 | 2375 | 0.21 | 0.28 | YES |
6 | GRB7 | GRB7 | GRB7 | 2495 | 0.2 | 0.32 | YES |
7 | MAPK8 | MAPK8 | MAPK8 | 2521 | 0.2 | 0.37 | YES |
8 | DOK1 | DOK1 | DOK1 | 2710 | 0.19 | 0.4 | YES |
9 | PIK3CA | PIK3CA | PIK3CA | 3182 | 0.17 | 0.41 | YES |
10 | DOK4 | DOK4 | DOK4 | 3296 | 0.16 | 0.45 | YES |
11 | GRB10 | GRB10 | GRB10 | 3781 | 0.15 | 0.45 | YES |
12 | SHC1 | SHC1 | SHC1 | 4369 | 0.13 | 0.45 | YES |
13 | RAP1A | RAP1A | RAP1A | 4527 | 0.12 | 0.47 | YES |
14 | DOK5 | DOK5 | DOK5 | 4948 | 0.11 | 0.47 | YES |
15 | CREB1 | CREB1 | CREB1 | 5356 | 0.1 | 0.48 | YES |
16 | PDLIM7 | PDLIM7 | PDLIM7 | 5379 | 0.1 | 0.5 | YES |
17 | CRK | CRK | CRK | 5725 | 0.094 | 0.5 | YES |
18 | JUN | JUN | JUN | 5880 | 0.09 | 0.51 | YES |
19 | FRS2 | FRS2 | FRS2 | 6692 | 0.073 | 0.48 | NO |
20 | PTPN11 | PTPN11 | PTPN11 | 7059 | 0.066 | 0.48 | NO |
21 | NCK1 | NCK1 | NCK1 | 7719 | 0.053 | 0.46 | NO |
22 | PXN | PXN | PXN | 7926 | 0.049 | 0.46 | NO |
23 | IRS1 | IRS1 | IRS1 | 8065 | 0.047 | 0.46 | NO |
24 | PTK2 | PTK2 | PTK2 | 8491 | 0.039 | 0.44 | NO |
25 | PIK3R1 | PIK3R1 | PIK3R1 | 9562 | 0.021 | 0.39 | NO |
26 | RAC1 | RAC1 | RAC1 | 9686 | 0.02 | 0.39 | NO |
27 | PRKACA | PRKACA | PRKACA | 9939 | 0.015 | 0.38 | NO |
28 | SOS1 | SOS1 | SOS1 | 10079 | 0.013 | 0.37 | NO |
29 | RET | RET | RET | 10402 | 0.0084 | 0.36 | NO |
30 | HRAS | HRAS | HRAS | 10631 | 0.0049 | 0.34 | NO |
31 | BCAR1 | BCAR1 | BCAR1 | 11087 | -0.002 | 0.32 | NO |
32 | RHOA | RHOA | RHOA | 11198 | -0.004 | 0.32 | NO |
33 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.3 | NO |
34 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.27 | NO |
35 | IRS2 | IRS2 | IRS2 | 12692 | -0.027 | 0.24 | NO |
36 | GRB2 | GRB2 | GRB2 | 12936 | -0.031 | 0.24 | NO |
37 | SRC | SRC | SRC | 14012 | -0.051 | 0.19 | NO |
38 | GAB1 | GAB1 | GAB1 | 14505 | -0.061 | 0.18 | NO |
39 | SHANK3 | SHANK3 | SHANK3 | 15288 | -0.081 | 0.15 | NO |
Figure S53. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID INTEGRIN1 PATHWAY.

Figure S54. Get High-res Image For the top 5 core enriched genes in the pathway: PID INTEGRIN1 PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S28. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | EPHB3 | EPHB3 | EPHB3 | 348 | 0.44 | 0.068 | YES |
2 | KALRN | KALRN | KALRN | 463 | 0.41 | 0.14 | YES |
3 | DNM1 | DNM1 | DNM1 | 589 | 0.38 | 0.21 | YES |
4 | EFNB2 | EFNB2 | EFNB2 | 595 | 0.38 | 0.29 | YES |
5 | EFNA5 | EFNA5 | EFNA5 | 936 | 0.33 | 0.33 | YES |
6 | GRIA1 | GRIA1 | GRIA1 | 1011 | 0.32 | 0.39 | YES |
7 | RASA1 | RASA1 | RASA1 | 2375 | 0.21 | 0.36 | YES |
8 | GRB7 | GRB7 | GRB7 | 2495 | 0.2 | 0.39 | YES |
9 | EFNB1 | EFNB1 | EFNB1 | 2641 | 0.2 | 0.42 | YES |
10 | PIK3CA | PIK3CA | PIK3CA | 3182 | 0.17 | 0.43 | YES |
11 | EPHB2 | EPHB2 | EPHB2 | 3197 | 0.17 | 0.46 | YES |
12 | TF | TF | TF | 4086 | 0.14 | 0.44 | YES |
13 | SHC1 | SHC1 | SHC1 | 4369 | 0.13 | 0.45 | YES |
14 | ROCK1 | ROCK1 | ROCK1 | 4469 | 0.12 | 0.46 | YES |
15 | RAP1A | RAP1A | RAP1A | 4527 | 0.12 | 0.49 | YES |
16 | MAP4K4 | MAP4K4 | MAP4K4 | 4608 | 0.12 | 0.51 | YES |
17 | EPHB4 | EPHB4 | EPHB4 | 5015 | 0.11 | 0.51 | YES |
18 | EFNB3 | EFNB3 | EFNB3 | 5017 | 0.11 | 0.53 | YES |
19 | KRAS | KRAS | KRAS | 5673 | 0.095 | 0.51 | YES |
20 | CRK | CRK | CRK | 5725 | 0.094 | 0.53 | YES |
21 | MAP2K1 | MAP2K1 | MAP2K1 | 5996 | 0.088 | 0.53 | YES |
22 | EPHB1 | EPHB1 | EPHB1 | 6393 | 0.079 | 0.52 | NO |
23 | ITSN1 | ITSN1 | ITSN1 | 6587 | 0.075 | 0.53 | NO |
24 | RAP1B | RAP1B | RAP1B | 7224 | 0.063 | 0.5 | NO |
25 | RRAS | RRAS | RRAS | 7442 | 0.058 | 0.5 | NO |
26 | NRAS | NRAS | NRAS | 7693 | 0.054 | 0.5 | NO |
27 | NCK1 | NCK1 | NCK1 | 7719 | 0.053 | 0.51 | NO |
28 | WASL | WASL | WASL | 7720 | 0.053 | 0.52 | NO |
29 | PXN | PXN | PXN | 7926 | 0.049 | 0.52 | NO |
30 | PTK2 | PTK2 | PTK2 | 8491 | 0.039 | 0.49 | NO |
31 | PIK3R1 | PIK3R1 | PIK3R1 | 9562 | 0.021 | 0.44 | NO |
32 | RAC1 | RAC1 | RAC1 | 9686 | 0.02 | 0.44 | NO |
33 | CDC42 | CDC42 | CDC42 | 10252 | 0.011 | 0.41 | NO |
34 | PAK1 | PAK1 | PAK1 | 10417 | 0.0082 | 0.4 | NO |
35 | HRAS | HRAS | HRAS | 10631 | 0.0049 | 0.39 | NO |
36 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.34 | NO |
37 | SYNJ1 | SYNJ1 | SYNJ1 | 11676 | -0.011 | 0.33 | NO |
38 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.31 | NO |
39 | GRB2 | GRB2 | GRB2 | 12936 | -0.031 | 0.27 | NO |
40 | SRC | SRC | SRC | 14012 | -0.051 | 0.22 | NO |
Figure S55. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID EPHBFWDPATHWAY.

Figure S56. Get High-res Image For the top 5 core enriched genes in the pathway: PID EPHBFWDPATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S29. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | CPE | CPE | CPE | 86 | 0.57 | 0.04 | YES |
2 | PCSK1 | PCSK1 | PCSK1 | 104 | 0.55 | 0.082 | YES |
3 | PAPPA | PAPPA | PAPPA | 183 | 0.5 | 0.12 | YES |
4 | IGFBP6 | IGFBP6 | IGFBP6 | 287 | 0.46 | 0.15 | YES |
5 | IGFBP3 | IGFBP3 | IGFBP3 | 416 | 0.42 | 0.17 | YES |
6 | STX1A | STX1A | STX1A | 456 | 0.41 | 0.2 | YES |
7 | MMP1 | MMP1 | MMP1 | 547 | 0.39 | 0.23 | YES |
8 | IGFBP5 | IGFBP5 | IGFBP5 | 604 | 0.38 | 0.25 | YES |
9 | IL8 | IL8 | IL8 | 740 | 0.36 | 0.28 | YES |
10 | ASNS | ASNS | ASNS | 922 | 0.33 | 0.29 | YES |
11 | IGFBP1 | IGFBP1 | IGFBP1 | 935 | 0.33 | 0.32 | YES |
12 | IGFBP2 | IGFBP2 | IGFBP2 | 979 | 0.33 | 0.34 | YES |
13 | IGF2BP1 | IGF2BP1 | IGF2BP1 | 1396 | 0.28 | 0.34 | YES |
14 | FKBP14 | FKBP14 | FKBP14 | 1672 | 0.26 | 0.34 | YES |
15 | PAPPA2 | PAPPA2 | PAPPA2 | 1895 | 0.24 | 0.35 | YES |
16 | IGFBP4 | IGFBP4 | IGFBP4 | 3034 | 0.18 | 0.3 | YES |
17 | CCL2 | CCL2 | CCL2 | 3367 | 0.16 | 0.29 | YES |
18 | IGF2BP2 | IGF2BP2 | IGF2BP2 | 3417 | 0.16 | 0.3 | YES |
19 | IGF2 | IGF2 | IGF2 | 3607 | 0.15 | 0.3 | YES |
20 | NFYB | NFYB | NFYB | 3855 | 0.14 | 0.3 | YES |
21 | CTSG | CTSG | CTSG | 4155 | 0.13 | 0.3 | YES |
22 | SHC1 | SHC1 | SHC1 | 4369 | 0.13 | 0.29 | YES |
23 | KIF5B | KIF5B | KIF5B | 4488 | 0.12 | 0.3 | YES |
24 | KLF4 | KLF4 | KLF4 | 4576 | 0.12 | 0.3 | YES |
25 | SLC30A7 | SLC30A7 | SLC30A7 | 4675 | 0.12 | 0.31 | YES |
26 | HYOU1 | HYOU1 | HYOU1 | 4722 | 0.12 | 0.31 | YES |
27 | DNAJC3 | DNAJC3 | DNAJC3 | 4884 | 0.12 | 0.31 | YES |
28 | IGF2BP3 | IGF2BP3 | IGF2BP3 | 4925 | 0.11 | 0.32 | YES |
29 | SNAP25 | SNAP25 | SNAP25 | 4952 | 0.11 | 0.33 | YES |
30 | PDIA6 | PDIA6 | PDIA6 | 5034 | 0.11 | 0.33 | YES |
31 | MMP2 | MMP2 | MMP2 | 5137 | 0.11 | 0.33 | YES |
32 | HSP90B1 | HSP90B1 | HSP90B1 | 5157 | 0.11 | 0.34 | YES |
33 | KDELR3 | KDELR3 | KDELR3 | 5163 | 0.11 | 0.35 | YES |
34 | EIF2AK3 | EIF2AK3 | EIF2AK3 | 5191 | 0.11 | 0.36 | YES |
35 | DNAJB9 | DNAJB9 | DNAJB9 | 5265 | 0.1 | 0.36 | YES |
36 | EDEM1 | EDEM1 | EDEM1 | 5469 | 0.1 | 0.36 | YES |
37 | C19orf10 | C19orf10 | C19orf10 | 5491 | 0.1 | 0.36 | YES |
38 | PDIA5 | PDIA5 | PDIA5 | 5535 | 0.099 | 0.37 | YES |
39 | DIS3 | DIS3 | DIS3 | 5561 | 0.098 | 0.38 | YES |
40 | HSPA5 | HSPA5 | HSPA5 | 5682 | 0.095 | 0.38 | YES |
41 | DNAJB11 | DNAJB11 | DNAJB11 | 5742 | 0.093 | 0.38 | YES |
42 | MBTPS1 | MBTPS1 | MBTPS1 | 5924 | 0.089 | 0.38 | NO |
43 | ATF3 | ATF3 | ATF3 | 6370 | 0.08 | 0.36 | NO |
44 | EXTL3 | EXTL3 | EXTL3 | 6568 | 0.075 | 0.35 | NO |
45 | SRPRB | SRPRB | SRPRB | 6690 | 0.073 | 0.35 | NO |
46 | TSPYL2 | TSPYL2 | TSPYL2 | 6737 | 0.072 | 0.36 | NO |
47 | SPCS3 | SPCS3 | SPCS3 | 6822 | 0.07 | 0.36 | NO |
48 | SLC30A5 | SLC30A5 | SLC30A5 | 6938 | 0.068 | 0.35 | NO |
49 | IGFALS | IGFALS | IGFALS | 6939 | 0.068 | 0.36 | NO |
50 | SERP1 | SERP1 | SERP1 | 7118 | 0.065 | 0.36 | NO |
51 | EXOSC8 | EXOSC8 | EXOSC8 | 7169 | 0.064 | 0.36 | NO |
52 | MBTPS2 | MBTPS2 | MBTPS2 | 7446 | 0.058 | 0.35 | NO |
53 | LEP | LEP | LEP | 7493 | 0.058 | 0.35 | NO |
54 | EXOC5 | EXOC5 | EXOC5 | 7538 | 0.057 | 0.35 | NO |
55 | EXOC1 | EXOC1 | EXOC1 | 7624 | 0.055 | 0.35 | NO |
56 | DCP2 | DCP2 | DCP2 | 7728 | 0.053 | 0.35 | NO |
57 | SULT1A3 | SULT1A3 | SULT1A3 | 7910 | 0.05 | 0.34 | NO |
58 | EXOC6 | EXOC6 | EXOC6 | 7929 | 0.049 | 0.34 | NO |
59 | EIF2S1 | EIF2S1 | EIF2S1 | 8246 | 0.044 | 0.33 | NO |
60 | CTDSP2 | CTDSP2 | CTDSP2 | 8308 | 0.042 | 0.33 | NO |
61 | SSR1 | SSR1 | SSR1 | 8680 | 0.036 | 0.31 | NO |
62 | ACADVL | ACADVL | ACADVL | 8873 | 0.033 | 0.3 | NO |
63 | ATF6 | ATF6 | ATF6 | 8912 | 0.032 | 0.3 | NO |
64 | XBP1 | XBP1 | XBP1 | 8959 | 0.031 | 0.3 | NO |
65 | DDIT3 | DDIT3 | DDIT3 | 8997 | 0.031 | 0.3 | NO |
66 | CALR | CALR | CALR | 9029 | 0.03 | 0.31 | NO |
67 | SPCS1 | SPCS1 | SPCS1 | 9155 | 0.028 | 0.3 | NO |
68 | ATF4 | ATF4 | ATF4 | 9383 | 0.024 | 0.29 | NO |
69 | ERN1 | ERN1 | ERN1 | 9454 | 0.023 | 0.29 | NO |
70 | EXOSC3 | EXOSC3 | EXOSC3 | 9603 | 0.021 | 0.28 | NO |
71 | SYVN1 | SYVN1 | SYVN1 | 9644 | 0.02 | 0.28 | NO |
72 | SRPR | SRPR | SRPR | 9673 | 0.02 | 0.28 | NO |
73 | SEC11A | SEC11A | SEC11A | 9818 | 0.017 | 0.27 | NO |
74 | GOSR2 | GOSR2 | GOSR2 | 9873 | 0.016 | 0.27 | NO |
75 | ZBTB17 | ZBTB17 | ZBTB17 | 10047 | 0.014 | 0.26 | NO |
76 | BCHE | BCHE | BCHE | 10098 | 0.013 | 0.26 | NO |
77 | HDGF | HDGF | HDGF | 10298 | 0.01 | 0.25 | NO |
78 | CUL7 | CUL7 | CUL7 | 10318 | 0.0099 | 0.25 | NO |
79 | EXOC4 | EXOC4 | EXOC4 | 10404 | 0.0083 | 0.25 | NO |
80 | GHRL | GHRL | GHRL | 10436 | 0.0079 | 0.25 | NO |
81 | ERO1L | ERO1L | ERO1L | 10514 | 0.0067 | 0.24 | NO |
82 | PPP2R5B | PPP2R5B | PPP2R5B | 10535 | 0.0064 | 0.24 | NO |
83 | MBOAT4 | MBOAT4 | MBOAT4 | 10677 | 0.0042 | 0.23 | NO |
84 | NFYA | NFYA | NFYA | 10839 | 0.0019 | 0.22 | NO |
85 | SEC31A | SEC31A | SEC31A | 11118 | -0.0025 | 0.21 | NO |
86 | EXOSC2 | EXOSC2 | EXOSC2 | 11125 | -0.0027 | 0.21 | NO |
87 | EXOSC9 | EXOSC9 | EXOSC9 | 11404 | -0.0073 | 0.2 | NO |
88 | YIF1A | YIF1A | YIF1A | 11442 | -0.0079 | 0.19 | NO |
89 | SPCS2 | SPCS2 | SPCS2 | 11497 | -0.0086 | 0.19 | NO |
90 | DCTN1 | DCTN1 | DCTN1 | 11668 | -0.011 | 0.18 | NO |
91 | EXOSC6 | EXOSC6 | EXOSC6 | 11674 | -0.011 | 0.18 | NO |
92 | LOC653566 | LOC653566 | LOC653566 | 11785 | -0.013 | 0.18 | NO |
93 | VAMP2 | VAMP2 | VAMP2 | 12068 | -0.017 | 0.16 | NO |
94 | ADD1 | ADD1 | ADD1 | 12146 | -0.018 | 0.16 | NO |
95 | PLA2G7 | PLA2G7 | PLA2G7 | 12157 | -0.018 | 0.16 | NO |
96 | GSK3A | GSK3A | GSK3A | 12325 | -0.021 | 0.15 | NO |
97 | PREB | PREB | PREB | 12410 | -0.022 | 0.15 | NO |
98 | TATDN2 | TATDN2 | TATDN2 | 12566 | -0.025 | 0.14 | NO |
99 | PCSK2 | PCSK2 | PCSK2 | 12940 | -0.031 | 0.13 | NO |
100 | EXOSC7 | EXOSC7 | EXOSC7 | 12958 | -0.031 | 0.13 | NO |
101 | EXOSC5 | EXOSC5 | EXOSC5 | 13121 | -0.034 | 0.12 | NO |
102 | EXOC8 | EXOC8 | EXOC8 | 13280 | -0.036 | 0.12 | NO |
103 | HERPUD1 | HERPUD1 | HERPUD1 | 13301 | -0.037 | 0.12 | NO |
104 | CXXC1 | CXXC1 | CXXC1 | 13375 | -0.038 | 0.12 | NO |
105 | TLN1 | TLN1 | TLN1 | 13511 | -0.041 | 0.11 | NO |
106 | KLHDC3 | KLHDC3 | KLHDC3 | 13628 | -0.043 | 0.11 | NO |
107 | LMNA | LMNA | LMNA | 14103 | -0.052 | 0.086 | NO |
108 | WFS1 | WFS1 | WFS1 | 14189 | -0.054 | 0.085 | NO |
109 | KHSRP | KHSRP | KHSRP | 14195 | -0.054 | 0.089 | NO |
110 | EXOC3 | EXOC3 | EXOC3 | 14228 | -0.055 | 0.092 | NO |
111 | PARN | PARN | PARN | 14264 | -0.056 | 0.094 | NO |
112 | SEC11C | SEC11C | SEC11C | 14267 | -0.056 | 0.098 | NO |
113 | ARFGAP1 | ARFGAP1 | ARFGAP1 | 14843 | -0.069 | 0.072 | NO |
114 | DDX11 | DDX11 | DDX11 | 15270 | -0.08 | 0.054 | NO |
115 | EXOSC1 | EXOSC1 | EXOSC1 | 15276 | -0.081 | 0.06 | NO |
116 | TPP1 | TPP1 | TPP1 | 15399 | -0.085 | 0.06 | NO |
117 | EXOSC4 | EXOSC4 | EXOSC4 | 15467 | -0.087 | 0.063 | NO |
118 | ATP6V0D1 | ATP6V0D1 | ATP6V0D1 | 15808 | -0.099 | 0.052 | NO |
119 | EXOC2 | EXOC2 | EXOC2 | 16177 | -0.12 | 0.04 | NO |
120 | MYO5A | MYO5A | MYO5A | 16941 | -0.17 | 0.011 | NO |
121 | GH1 | GH1 | GH1 | 16984 | -0.17 | 0.022 | NO |
122 | WIPI1 | WIPI1 | WIPI1 | 17228 | -0.2 | 0.024 | NO |
123 | RAB27A | RAB27A | RAB27A | 17638 | -0.28 | 0.022 | NO |
Figure S57. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID RET PATHWAY.

Figure S58. Get High-res Image For the top 5 core enriched genes in the pathway: PID RET PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S30. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | INHBA | INHBA | INHBA | 52 | 0.62 | 0.045 | YES |
2 | BMP2 | BMP2 | BMP2 | 130 | 0.53 | 0.082 | YES |
3 | FST | FST | FST | 226 | 0.48 | 0.11 | YES |
4 | THBS2 | THBS2 | THBS2 | 422 | 0.42 | 0.14 | YES |
5 | LTBP1 | LTBP1 | LTBP1 | 458 | 0.41 | 0.16 | YES |
6 | ID1 | ID1 | ID1 | 610 | 0.38 | 0.19 | YES |
7 | BMP6 | BMP6 | BMP6 | 646 | 0.37 | 0.21 | YES |
8 | ID3 | ID3 | ID3 | 730 | 0.36 | 0.24 | YES |
9 | DCN | DCN | DCN | 791 | 0.35 | 0.26 | YES |
10 | BMP8A | BMP8A | BMP8A | 808 | 0.35 | 0.29 | YES |
11 | BMP5 | BMP5 | BMP5 | 816 | 0.35 | 0.31 | YES |
12 | INHBB | INHBB | INHBB | 859 | 0.34 | 0.34 | YES |
13 | THBS4 | THBS4 | THBS4 | 1060 | 0.32 | 0.35 | YES |
14 | CHRD | CHRD | CHRD | 1149 | 0.31 | 0.37 | YES |
15 | ID4 | ID4 | ID4 | 1165 | 0.3 | 0.39 | YES |
16 | THBS1 | THBS1 | THBS1 | 1224 | 0.3 | 0.41 | YES |
17 | TGFB3 | TGFB3 | TGFB3 | 1273 | 0.3 | 0.43 | YES |
18 | TGFB2 | TGFB2 | TGFB2 | 1381 | 0.28 | 0.45 | YES |
19 | SMAD1 | SMAD1 | SMAD1 | 1416 | 0.28 | 0.47 | YES |
20 | BMP8B | BMP8B | BMP8B | 1462 | 0.28 | 0.49 | YES |
21 | SMURF2 | SMURF2 | SMURF2 | 1787 | 0.25 | 0.49 | YES |
22 | E2F5 | E2F5 | E2F5 | 2417 | 0.21 | 0.47 | YES |
23 | SMAD9 | SMAD9 | SMAD9 | 2433 | 0.21 | 0.48 | YES |
24 | ROCK2 | ROCK2 | ROCK2 | 2506 | 0.2 | 0.5 | YES |
25 | GDF6 | GDF6 | GDF6 | 2619 | 0.2 | 0.5 | YES |
26 | ACVR2A | ACVR2A | ACVR2A | 2691 | 0.19 | 0.52 | YES |
27 | COMP | COMP | COMP | 2707 | 0.19 | 0.53 | YES |
28 | ACVR1 | ACVR1 | ACVR1 | 2749 | 0.19 | 0.54 | YES |
29 | BMP7 | BMP7 | BMP7 | 3193 | 0.17 | 0.53 | YES |
30 | THBS3 | THBS3 | THBS3 | 3257 | 0.17 | 0.54 | YES |
31 | AMH | AMH | AMH | 3360 | 0.16 | 0.55 | YES |
32 | NOG | NOG | NOG | 3529 | 0.16 | 0.55 | YES |
33 | BMPR1A | BMPR1A | BMPR1A | 3647 | 0.15 | 0.56 | YES |
34 | SMAD5 | SMAD5 | SMAD5 | 3805 | 0.15 | 0.56 | YES |
35 | ID2 | ID2 | ID2 | 4372 | 0.13 | 0.54 | NO |
36 | LEFTY1 | LEFTY1 | LEFTY1 | 4426 | 0.13 | 0.54 | NO |
37 | ROCK1 | ROCK1 | ROCK1 | 4469 | 0.12 | 0.55 | NO |
38 | TGFB1 | TGFB1 | TGFB1 | 4652 | 0.12 | 0.55 | NO |
39 | SMAD7 | SMAD7 | SMAD7 | 4694 | 0.12 | 0.56 | NO |
40 | GDF5 | GDF5 | GDF5 | 4870 | 0.12 | 0.56 | NO |
41 | BMP4 | BMP4 | BMP4 | 5159 | 0.11 | 0.55 | NO |
42 | TGFBR2 | TGFBR2 | TGFBR2 | 5402 | 0.1 | 0.54 | NO |
43 | CDKN2B | CDKN2B | CDKN2B | 5460 | 0.1 | 0.55 | NO |
44 | PPP2R1B | PPP2R1B | PPP2R1B | 5553 | 0.098 | 0.55 | NO |
45 | ACVRL1 | ACVRL1 | ACVRL1 | 5785 | 0.093 | 0.54 | NO |
46 | NODAL | NODAL | NODAL | 5800 | 0.092 | 0.55 | NO |
47 | RBL1 | RBL1 | RBL1 | 6259 | 0.082 | 0.53 | NO |
48 | BMPR1B | BMPR1B | BMPR1B | 6292 | 0.081 | 0.54 | NO |
49 | SMAD4 | SMAD4 | SMAD4 | 6718 | 0.073 | 0.52 | NO |
50 | RPS6KB1 | RPS6KB1 | RPS6KB1 | 6772 | 0.072 | 0.52 | NO |
51 | LEFTY2 | LEFTY2 | LEFTY2 | 6812 | 0.071 | 0.52 | NO |
52 | RBL2 | RBL2 | RBL2 | 7100 | 0.065 | 0.51 | NO |
53 | INHBE | INHBE | INHBE | 7407 | 0.059 | 0.5 | NO |
54 | PPP2CB | PPP2CB | PPP2CB | 8310 | 0.042 | 0.45 | NO |
55 | ZFYVE9 | ZFYVE9 | ZFYVE9 | 8370 | 0.041 | 0.45 | NO |
56 | ACVR1C | ACVR1C | ACVR1C | 9119 | 0.028 | 0.41 | NO |
57 | ACVR2B | ACVR2B | ACVR2B | 9425 | 0.024 | 0.4 | NO |
58 | CUL1 | CUL1 | CUL1 | 9479 | 0.023 | 0.4 | NO |
59 | E2F4 | E2F4 | E2F4 | 9487 | 0.022 | 0.4 | NO |
60 | SMAD6 | SMAD6 | SMAD6 | 9795 | 0.018 | 0.38 | NO |
61 | BMPR2 | BMPR2 | BMPR2 | 10054 | 0.014 | 0.37 | NO |
62 | SP1 | SP1 | SP1 | 10111 | 0.013 | 0.37 | NO |
63 | SMAD3 | SMAD3 | SMAD3 | 10157 | 0.012 | 0.36 | NO |
64 | TFDP1 | TFDP1 | TFDP1 | 10249 | 0.011 | 0.36 | NO |
65 | INHBC | INHBC | INHBC | 10467 | 0.0073 | 0.35 | NO |
66 | SKP1 | SKP1 | SKP1 | 10617 | 0.0052 | 0.34 | NO |
67 | PPP2CA | PPP2CA | PPP2CA | 10702 | 0.0039 | 0.34 | NO |
68 | SMAD2 | SMAD2 | SMAD2 | 10992 | -0.00054 | 0.32 | NO |
69 | RHOA | RHOA | RHOA | 11198 | -0.004 | 0.31 | NO |
70 | MAPK3 | MAPK3 | MAPK3 | 11492 | -0.0085 | 0.29 | NO |
71 | PITX2 | PITX2 | PITX2 | 11524 | -0.0091 | 0.29 | NO |
72 | EP300 | EP300 | EP300 | 11820 | -0.013 | 0.28 | NO |
73 | CREBBP | CREBBP | CREBBP | 11943 | -0.015 | 0.27 | NO |
74 | MAPK1 | MAPK1 | MAPK1 | 12197 | -0.019 | 0.26 | NO |
75 | TGFBR1 | TGFBR1 | TGFBR1 | 12281 | -0.02 | 0.26 | NO |
76 | RBX1 | RBX1 | RBX1 | 12376 | -0.022 | 0.25 | NO |
77 | PPP2R1A | PPP2R1A | PPP2R1A | 12767 | -0.028 | 0.23 | NO |
78 | SMURF1 | SMURF1 | SMURF1 | 12771 | -0.028 | 0.24 | NO |
79 | GDF7 | GDF7 | GDF7 | 12834 | -0.029 | 0.23 | NO |
80 | ZFYVE16 | ZFYVE16 | ZFYVE16 | 14390 | -0.058 | 0.15 | NO |
81 | RPS6KB2 | RPS6KB2 | RPS6KB2 | 15110 | -0.076 | 0.12 | NO |
82 | TNF | TNF | TNF | 15631 | -0.092 | 0.096 | NO |
83 | MYC | MYC | MYC | 16753 | -0.15 | 0.045 | NO |
84 | AMHR2 | AMHR2 | AMHR2 | 16867 | -0.16 | 0.051 | NO |
85 | IFNG | IFNG | IFNG | 17081 | -0.18 | 0.053 | NO |
Figure S59. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID NCADHERINPATHWAY.

Figure S60. Get High-res Image For the top 5 core enriched genes in the pathway: PID NCADHERINPATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

For the top enriched genes, if you want to check whether they are
-
up-regulated, please check the list of up-regulated genes
-
down-regulated, please check the list of down-regulated genes
For the top enriched genes, if you want to check whether they are
-
highly expressed genes, please check the list of high (top 30%) expressed genes
-
low expressed genes, please check the list of low (bottom 30%) expressed genes
An expression pattern of top(30%)/middle(30%)/low(30%) in this subtype against other subtypes is available in a heatmap
For the top enriched genes, if you want to check whether they are
-
significantly differently expressed genes by eBayes lm fit, please check the list of significant genes
Table 6. Get Full Table This table shows top 10 pathways which are significantly enriched in cluster clus4. It displays only significant gene sets satisfying nom.p.val.threshold (-1), fwer.p.val.threshold (-1) , fdr.q.val.threshold (0.25) and the default table is sorted by Normalized Enrichment Score (NES). Further details on NES statistics, please visit The Broad GSEA website.
GeneSet(GS) | Size(#genes) | genes.ES.table | ES | NES | NOM.p.val | FDR.q.val | FWER.p.val | Tag.. | Gene.. | Signal | FDR..median. | glob.p.val |
---|---|---|---|---|---|---|---|---|---|---|---|---|
KEGG LYSINE DEGRADATION | 44 | genes.ES.table | 0.37 | 1.6 | 0.033 | 0.25 | 0.95 | 0.5 | 0.29 | 0.36 | 0.15 | 0.027 |
KEGG RIBOSOME | 85 | genes.ES.table | 0.64 | 1.7 | 0.046 | 0.22 | 0.77 | 0.87 | 0.33 | 0.59 | 0.095 | 0.039 |
KEGG BASAL TRANSCRIPTION FACTORS | 34 | genes.ES.table | 0.52 | 1.7 | 0.021 | 0.19 | 0.82 | 0.62 | 0.33 | 0.41 | 0.084 | 0.024 |
KEGG SPLICEOSOME | 114 | genes.ES.table | 0.42 | 1.9 | 0.023 | 0.17 | 0.35 | 0.57 | 0.39 | 0.35 | 0 | 0.036 |
PID ATM PATHWAY | 34 | genes.ES.table | 0.52 | 1.7 | 0.042 | 0.19 | 0.8 | 0.59 | 0.32 | 0.4 | 0.085 | 0.027 |
PID RETINOIC ACID PATHWAY | 30 | genes.ES.table | 0.45 | 1.6 | 0.039 | 0.23 | 0.95 | 0.43 | 0.26 | 0.32 | 0.15 | 0.02 |
PID BARD1PATHWAY | 29 | genes.ES.table | 0.51 | 1.6 | 0.1 | 0.25 | 0.94 | 0.55 | 0.3 | 0.38 | 0.16 | 0.029 |
PID P53REGULATIONPATHWAY | 58 | genes.ES.table | 0.36 | 1.6 | 0.035 | 0.2 | 0.91 | 0.4 | 0.3 | 0.28 | 0.11 | 0.021 |
REACTOME TRANSLATION | 146 | genes.ES.table | 0.51 | 1.7 | 0.082 | 0.21 | 0.78 | 0.73 | 0.37 | 0.47 | 0.092 | 0.036 |
REACTOME FORMATION OF THE TERNARY COMPLEX AND SUBSEQUENTLY THE 43S COMPLEX | 49 | genes.ES.table | 0.6 | 1.7 | 0.051 | 0.18 | 0.83 | 0.8 | 0.34 | 0.52 | 0.083 | 0.019 |
Table S31. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | RPL36A | RPL36A | RPL36A | 919 | 0.18 | -0.037 | YES |
2 | RPS27 | RPS27 | RPS27 | 1011 | 0.17 | -0.028 | YES |
3 | RPL7 | RPL7 | RPL7 | 1214 | 0.16 | -0.027 | YES |
4 | DCP2 | DCP2 | DCP2 | 1362 | 0.15 | -0.023 | YES |
5 | PRKCA | PRKCA | PRKCA | 1378 | 0.15 | -0.012 | YES |
6 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | -0.00036 | YES |
7 | RPL21 | RPL21 | RPL21 | 1606 | 0.13 | -0.0019 | YES |
8 | RPL9 | RPL9 | RPL9 | 1630 | 0.13 | 0.0076 | YES |
9 | RPS3A | RPS3A | RPS3A | 1684 | 0.13 | 0.015 | YES |
10 | RPL39 | RPL39 | RPL39 | 1806 | 0.12 | 0.018 | YES |
11 | PABPC1 | PABPC1 | PABPC1 | 1834 | 0.12 | 0.027 | YES |
12 | EXOSC8 | EXOSC8 | EXOSC8 | 1968 | 0.12 | 0.029 | YES |
13 | SMN1 | SMN1 | SMN1 | 2083 | 0.11 | 0.032 | YES |
14 | TGS1 | TGS1 | TGS1 | 2088 | 0.11 | 0.041 | YES |
15 | RPS7 | RPS7 | RPS7 | 2333 | 0.1 | 0.035 | YES |
16 | NUP133 | NUP133 | NUP133 | 2383 | 0.1 | 0.041 | YES |
17 | RPS12 | RPS12 | RPS12 | 2433 | 0.1 | 0.046 | YES |
18 | SNRPD1 | SNRPD1 | SNRPD1 | 2442 | 0.1 | 0.054 | YES |
19 | PAIP1 | PAIP1 | PAIP1 | 2524 | 0.099 | 0.058 | YES |
20 | RPS18 | RPS18 | RPS18 | 2533 | 0.099 | 0.065 | YES |
21 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | 0.069 | YES |
22 | RPSAP9 | RPSAP9 | RPSAP9 | 2706 | 0.094 | 0.071 | YES |
23 | RPS23 | RPS23 | RPS23 | 2738 | 0.093 | 0.077 | YES |
24 | RPL30 | RPL30 | RPL30 | 2787 | 0.092 | 0.081 | YES |
25 | EIF4B | EIF4B | EIF4B | 2852 | 0.09 | 0.085 | YES |
26 | RPS10 | RPS10 | RPS10 | 2869 | 0.09 | 0.091 | YES |
27 | RPL17 | RPL17 | RPL17 | 2876 | 0.09 | 0.098 | YES |
28 | RPL22 | RPL22 | RPL22 | 2917 | 0.088 | 0.1 | YES |
29 | RPS20 | RPS20 | RPS20 | 2996 | 0.086 | 0.11 | YES |
30 | RPL26 | RPL26 | RPL26 | 3006 | 0.086 | 0.11 | YES |
31 | RPL8 | RPL8 | RPL8 | 3011 | 0.086 | 0.12 | YES |
32 | RPL10A | RPL10A | RPL10A | 3034 | 0.085 | 0.12 | YES |
33 | RPL34 | RPL34 | RPL34 | 3039 | 0.085 | 0.13 | YES |
34 | DIS3 | DIS3 | DIS3 | 3045 | 0.085 | 0.14 | YES |
35 | UPF3A | UPF3A | UPF3A | 3049 | 0.085 | 0.14 | YES |
36 | CNOT6 | CNOT6 | CNOT6 | 3056 | 0.085 | 0.15 | YES |
37 | NUP153 | NUP153 | NUP153 | 3074 | 0.085 | 0.16 | YES |
38 | RPL23 | RPL23 | RPL23 | 3186 | 0.082 | 0.16 | YES |
39 | RPL5 | RPL5 | RPL5 | 3230 | 0.081 | 0.16 | YES |
40 | RPL6 | RPL6 | RPL6 | 3280 | 0.08 | 0.16 | YES |
41 | RPS8 | RPS8 | RPS8 | 3378 | 0.078 | 0.17 | YES |
42 | CLNS1A | CLNS1A | CLNS1A | 3381 | 0.078 | 0.17 | YES |
43 | RPS6 | RPS6 | RPS6 | 3504 | 0.075 | 0.17 | YES |
44 | RPS26 | RPS26 | RPS26 | 3519 | 0.074 | 0.18 | YES |
45 | RPS24 | RPS24 | RPS24 | 3538 | 0.074 | 0.18 | YES |
46 | ANP32A | ANP32A | ANP32A | 3645 | 0.071 | 0.18 | YES |
47 | GEMIN5 | GEMIN5 | GEMIN5 | 3663 | 0.071 | 0.19 | YES |
48 | TPR | TPR | TPR | 3674 | 0.071 | 0.19 | YES |
49 | EXOSC2 | EXOSC2 | EXOSC2 | 3683 | 0.07 | 0.2 | YES |
50 | EIF4A2 | EIF4A2 | EIF4A2 | 3688 | 0.07 | 0.2 | YES |
51 | RPL13A | RPL13A | RPL13A | 3735 | 0.069 | 0.2 | YES |
52 | SMN2 | SMN2 | SMN2 | 3746 | 0.069 | 0.21 | YES |
53 | RPS17 | RPS17 | RPS17 | 3826 | 0.068 | 0.21 | YES |
54 | RPS4X | RPS4X | RPS4X | 3829 | 0.068 | 0.22 | YES |
55 | RPL4 | RPL4 | RPL4 | 3835 | 0.067 | 0.22 | YES |
56 | GSPT2 | GSPT2 | GSPT2 | 3851 | 0.067 | 0.23 | YES |
57 | UPF2 | UPF2 | UPF2 | 3877 | 0.067 | 0.23 | YES |
58 | RPL3 | RPL3 | RPL3 | 3889 | 0.066 | 0.24 | YES |
59 | LSM6 | LSM6 | LSM6 | 3944 | 0.066 | 0.24 | YES |
60 | RPL31 | RPL31 | RPL31 | 3949 | 0.065 | 0.24 | YES |
61 | RPL12 | RPL12 | RPL12 | 3978 | 0.065 | 0.25 | YES |
62 | RPS3 | RPS3 | RPS3 | 3990 | 0.065 | 0.25 | YES |
63 | PSMA8 | PSMA8 | PSMA8 | 4010 | 0.064 | 0.26 | YES |
64 | RPL38 | RPL38 | RPL38 | 4039 | 0.064 | 0.26 | YES |
65 | RPS14 | RPS14 | RPS14 | 4056 | 0.064 | 0.26 | YES |
66 | RPS15A | RPS15A | RPS15A | 4070 | 0.063 | 0.27 | YES |
67 | RPS19 | RPS19 | RPS19 | 4166 | 0.061 | 0.27 | YES |
68 | RPS27A | RPS27A | RPS27A | 4186 | 0.061 | 0.27 | YES |
69 | RPS21 | RPS21 | RPS21 | 4216 | 0.061 | 0.27 | YES |
70 | NUP43 | NUP43 | NUP43 | 4261 | 0.06 | 0.28 | YES |
71 | RPS29 | RPS29 | RPS29 | 4284 | 0.059 | 0.28 | YES |
72 | NUP35 | NUP35 | NUP35 | 4302 | 0.059 | 0.28 | YES |
73 | EXOSC4 | EXOSC4 | EXOSC4 | 4314 | 0.059 | 0.29 | YES |
74 | RPS5 | RPS5 | RPS5 | 4329 | 0.058 | 0.29 | YES |
75 | SEH1L | SEH1L | SEH1L | 4336 | 0.058 | 0.3 | YES |
76 | PSMC6 | PSMC6 | PSMC6 | 4339 | 0.058 | 0.3 | YES |
77 | EXOSC5 | EXOSC5 | EXOSC5 | 4347 | 0.058 | 0.3 | YES |
78 | RPL7A | RPL7A | RPL7A | 4365 | 0.058 | 0.31 | YES |
79 | MAPK14 | MAPK14 | MAPK14 | 4371 | 0.058 | 0.31 | YES |
80 | RPL23A | RPL23A | RPL23A | 4386 | 0.057 | 0.32 | YES |
81 | DCP1B | DCP1B | DCP1B | 4425 | 0.057 | 0.32 | YES |
82 | NUPL1 | NUPL1 | NUPL1 | 4485 | 0.056 | 0.32 | YES |
83 | RPL14 | RPL14 | RPL14 | 4551 | 0.055 | 0.32 | YES |
84 | KHSRP | KHSRP | KHSRP | 4578 | 0.054 | 0.32 | YES |
85 | PPP2R2A | PPP2R2A | PPP2R2A | 4603 | 0.054 | 0.33 | YES |
86 | RPL37 | RPL37 | RPL37 | 4658 | 0.053 | 0.33 | YES |
87 | NUP107 | NUP107 | NUP107 | 4673 | 0.053 | 0.33 | YES |
88 | RPL24 | RPL24 | RPL24 | 4701 | 0.053 | 0.33 | YES |
89 | LSM2 | LSM2 | LSM2 | 4703 | 0.052 | 0.34 | YES |
90 | RPL35A | RPL35A | RPL35A | 4720 | 0.052 | 0.34 | YES |
91 | RPS16 | RPS16 | RPS16 | 4737 | 0.052 | 0.34 | YES |
92 | EXOSC3 | EXOSC3 | EXOSC3 | 4751 | 0.052 | 0.35 | YES |
93 | RPL29 | RPL29 | RPL29 | 4763 | 0.052 | 0.35 | YES |
94 | RPS25 | RPS25 | RPS25 | 4769 | 0.051 | 0.36 | YES |
95 | NUP54 | NUP54 | NUP54 | 4772 | 0.051 | 0.36 | YES |
96 | GEMIN7 | GEMIN7 | GEMIN7 | 4827 | 0.05 | 0.36 | YES |
97 | CNOT8 | CNOT8 | CNOT8 | 4835 | 0.05 | 0.36 | YES |
98 | RPL18 | RPL18 | RPL18 | 4906 | 0.049 | 0.36 | YES |
99 | RPL36 | RPL36 | RPL36 | 4907 | 0.049 | 0.37 | YES |
100 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.37 | YES |
101 | RPL37A | RPL37A | RPL37A | 4981 | 0.048 | 0.37 | YES |
102 | XPO1 | XPO1 | XPO1 | 4996 | 0.048 | 0.38 | YES |
103 | PSMD10 | PSMD10 | PSMD10 | 4997 | 0.048 | 0.38 | YES |
104 | RPL32 | RPL32 | RPL32 | 5021 | 0.048 | 0.38 | YES |
105 | CNOT7 | CNOT7 | CNOT7 | 5039 | 0.047 | 0.38 | YES |
106 | RPL11 | RPL11 | RPL11 | 5057 | 0.047 | 0.39 | YES |
107 | RPS13 | RPS13 | RPS13 | 5119 | 0.046 | 0.39 | YES |
108 | RPL19 | RPL19 | RPL19 | 5136 | 0.046 | 0.39 | YES |
109 | PSMB4 | PSMB4 | PSMB4 | 5237 | 0.045 | 0.39 | YES |
110 | RPLP1 | RPLP1 | RPLP1 | 5252 | 0.044 | 0.39 | YES |
111 | RPS11 | RPS11 | RPS11 | 5255 | 0.044 | 0.4 | YES |
112 | RPL18A | RPL18A | RPL18A | 5283 | 0.044 | 0.4 | YES |
113 | RPS2 | RPS2 | RPS2 | 5290 | 0.044 | 0.4 | YES |
114 | PHAX | PHAX | PHAX | 5324 | 0.043 | 0.4 | YES |
115 | RPLP0 | RPLP0 | RPLP0 | 5343 | 0.043 | 0.4 | YES |
116 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.41 | YES |
117 | RPL15 | RPL15 | RPL15 | 5370 | 0.043 | 0.41 | YES |
118 | HNRNPD | HNRNPD | HNRNPD | 5462 | 0.041 | 0.41 | YES |
119 | RPS9 | RPS9 | RPS9 | 5501 | 0.041 | 0.41 | YES |
120 | RPL13 | RPL13 | RPL13 | 5526 | 0.04 | 0.41 | YES |
121 | DDX20 | DDX20 | DDX20 | 5560 | 0.04 | 0.41 | YES |
122 | DDX6 | DDX6 | DDX6 | 5589 | 0.04 | 0.41 | YES |
123 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.41 | YES |
124 | DCP1A | DCP1A | DCP1A | 5691 | 0.038 | 0.42 | YES |
125 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.42 | YES |
126 | RPL27 | RPL27 | RPL27 | 5712 | 0.038 | 0.42 | YES |
127 | HSPA1B | HSPA1B | HSPA1B | 5721 | 0.038 | 0.42 | YES |
128 | PRMT5 | PRMT5 | PRMT5 | 5746 | 0.038 | 0.42 | YES |
129 | RPL27A | RPL27A | RPL27A | 5771 | 0.037 | 0.43 | YES |
130 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.43 | YES |
131 | RPS15 | RPS15 | RPS15 | 5875 | 0.036 | 0.43 | YES |
132 | SMG5 | SMG5 | SMG5 | 5892 | 0.036 | 0.43 | YES |
133 | GEMIN4 | GEMIN4 | GEMIN4 | 5895 | 0.036 | 0.43 | YES |
134 | EIF4E | EIF4E | EIF4E | 5918 | 0.035 | 0.43 | YES |
135 | RPL35 | RPL35 | RPL35 | 5939 | 0.035 | 0.43 | YES |
136 | LSM5 | LSM5 | LSM5 | 5945 | 0.035 | 0.44 | YES |
137 | RPL41 | RPL41 | RPL41 | 5947 | 0.035 | 0.44 | YES |
138 | ETF1 | ETF1 | ETF1 | 6067 | 0.033 | 0.44 | YES |
139 | RPS28 | RPS28 | RPS28 | 6078 | 0.033 | 0.44 | YES |
140 | NUPL2 | NUPL2 | NUPL2 | 6137 | 0.032 | 0.44 | YES |
141 | PSMA4 | PSMA4 | PSMA4 | 6160 | 0.032 | 0.44 | YES |
142 | RPSA | RPSA | RPSA | 6191 | 0.032 | 0.44 | YES |
143 | NUP88 | NUP88 | NUP88 | 6196 | 0.032 | 0.44 | YES |
144 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.44 | YES |
145 | AAAS | AAAS | AAAS | 6275 | 0.03 | 0.44 | YES |
146 | ZFP36L1 | ZFP36L1 | ZFP36L1 | 6326 | 0.03 | 0.44 | YES |
147 | SNRPD2 | SNRPD2 | SNRPD2 | 6381 | 0.029 | 0.44 | YES |
148 | SNUPN | SNUPN | SNUPN | 6401 | 0.029 | 0.44 | YES |
149 | EXOSC7 | EXOSC7 | EXOSC7 | 6431 | 0.028 | 0.44 | YES |
150 | RPL10 | RPL10 | RPL10 | 6540 | 0.027 | 0.44 | NO |
151 | SMG7 | SMG7 | SMG7 | 6721 | 0.024 | 0.43 | NO |
152 | CNOT2 | CNOT2 | CNOT2 | 6736 | 0.024 | 0.43 | NO |
153 | PSMD5 | PSMD5 | PSMD5 | 6789 | 0.024 | 0.43 | NO |
154 | NUP85 | NUP85 | NUP85 | 6959 | 0.021 | 0.42 | NO |
155 | PPP2CA | PPP2CA | PPP2CA | 6970 | 0.021 | 0.42 | NO |
156 | HSPA8 | HSPA8 | HSPA8 | 6986 | 0.021 | 0.42 | NO |
157 | EIF4A3 | EIF4A3 | EIF4A3 | 7000 | 0.021 | 0.43 | NO |
158 | ELAVL1 | ELAVL1 | ELAVL1 | 7068 | 0.02 | 0.42 | NO |
159 | PSMF1 | PSMF1 | PSMF1 | 7099 | 0.02 | 0.42 | NO |
160 | TNPO1 | TNPO1 | TNPO1 | 7122 | 0.019 | 0.42 | NO |
161 | PSMA3 | PSMA3 | PSMA3 | 7222 | 0.018 | 0.42 | NO |
162 | PSMB7 | PSMB7 | PSMB7 | 7234 | 0.018 | 0.42 | NO |
163 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.42 | NO |
164 | RAE1 | RAE1 | RAE1 | 7320 | 0.016 | 0.42 | NO |
165 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.42 | NO |
166 | NUP37 | NUP37 | NUP37 | 7365 | 0.016 | 0.42 | NO |
167 | YWHAZ | YWHAZ | YWHAZ | 7397 | 0.015 | 0.42 | NO |
168 | PPP2R1A | PPP2R1A | PPP2R1A | 7427 | 0.015 | 0.42 | NO |
169 | PSMD4 | PSMD4 | PSMD4 | 7536 | 0.014 | 0.41 | NO |
170 | PSMA1 | PSMA1 | PSMA1 | 7581 | 0.013 | 0.41 | NO |
171 | RPLP2 | RPLP2 | RPLP2 | 7610 | 0.013 | 0.41 | NO |
172 | RPL28 | RPL28 | RPL28 | 7615 | 0.013 | 0.41 | NO |
173 | LSM4 | LSM4 | LSM4 | 7726 | 0.012 | 0.41 | NO |
174 | XRN1 | XRN1 | XRN1 | 7735 | 0.011 | 0.41 | NO |
175 | PARN | PARN | PARN | 7893 | 0.0093 | 0.4 | NO |
176 | POM121 | POM121 | POM121 | 7942 | 0.0088 | 0.4 | NO |
177 | EIF4A1 | EIF4A1 | EIF4A1 | 7945 | 0.0087 | 0.4 | NO |
178 | PRKCD | PRKCD | PRKCD | 7965 | 0.0086 | 0.4 | NO |
179 | SMG1 | SMG1 | SMG1 | 8134 | 0.0063 | 0.39 | NO |
180 | CASC3 | CASC3 | CASC3 | 8151 | 0.006 | 0.39 | NO |
181 | EDC3 | EDC3 | EDC3 | 8162 | 0.0058 | 0.39 | NO |
182 | CNOT4 | CNOT4 | CNOT4 | 8167 | 0.0058 | 0.39 | NO |
183 | SMG6 | SMG6 | SMG6 | 8171 | 0.0057 | 0.39 | NO |
184 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.39 | NO |
185 | UBA52 | UBA52 | UBA52 | 8183 | 0.0056 | 0.39 | NO |
186 | RANBP2 | RANBP2 | RANBP2 | 8229 | 0.0051 | 0.39 | NO |
187 | HSPB1 | HSPB1 | HSPB1 | 8305 | 0.0043 | 0.38 | NO |
188 | NUP62 | NUP62 | NUP62 | 8411 | 0.0028 | 0.38 | NO |
189 | NUP50 | NUP50 | NUP50 | 8461 | 0.0023 | 0.38 | NO |
190 | PSMC1 | PSMC1 | PSMC1 | 8484 | 0.002 | 0.37 | NO |
191 | PSMA6 | PSMA6 | PSMA6 | 8490 | 0.002 | 0.37 | NO |
192 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.37 | NO |
193 | PSMB1 | PSMB1 | PSMB1 | 8613 | 0.00026 | 0.37 | NO |
194 | PSMB5 | PSMB5 | PSMB5 | 8669 | -0.00052 | 0.36 | NO |
195 | FAU | FAU | FAU | 8710 | -0.0012 | 0.36 | NO |
196 | PSMA2 | PSMA2 | PSMA2 | 8764 | -0.0018 | 0.36 | NO |
197 | LSM3 | LSM3 | LSM3 | 8956 | -0.0039 | 0.35 | NO |
198 | WDR77 | WDR77 | WDR77 | 9102 | -0.0058 | 0.34 | NO |
199 | AKT1 | AKT1 | AKT1 | 9125 | -0.0062 | 0.34 | NO |
200 | EDC4 | EDC4 | EDC4 | 9130 | -0.0063 | 0.34 | NO |
201 | CNOT3 | CNOT3 | CNOT3 | 9131 | -0.0063 | 0.34 | NO |
202 | EXOSC9 | EXOSC9 | EXOSC9 | 9150 | -0.0064 | 0.34 | NO |
203 | C2orf29 | C2orf29 | C2orf29 | 9190 | -0.007 | 0.34 | NO |
204 | RPS4Y1 | RPS4Y1 | RPS4Y1 | 9221 | -0.0074 | 0.34 | NO |
205 | LSM1 | LSM1 | LSM1 | 9285 | -0.0085 | 0.34 | NO |
206 | YWHAB | YWHAB | YWHAB | 9333 | -0.0091 | 0.33 | NO |
207 | PSMD12 | PSMD12 | PSMD12 | 9353 | -0.0095 | 0.33 | NO |
208 | NUP155 | NUP155 | NUP155 | 9409 | -0.01 | 0.33 | NO |
209 | GEMIN6 | GEMIN6 | GEMIN6 | 9444 | -0.011 | 0.33 | NO |
210 | PSMB2 | PSMB2 | PSMB2 | 9465 | -0.011 | 0.33 | NO |
211 | PSME1 | PSME1 | PSME1 | 9570 | -0.012 | 0.32 | NO |
212 | DCPS | DCPS | DCPS | 9655 | -0.014 | 0.32 | NO |
213 | NUP205 | NUP205 | NUP205 | 9681 | -0.014 | 0.32 | NO |
214 | EXOSC6 | EXOSC6 | EXOSC6 | 9797 | -0.016 | 0.32 | NO |
215 | PSME2 | PSME2 | PSME2 | 10052 | -0.019 | 0.3 | NO |
216 | PSMD7 | PSMD7 | PSMD7 | 10056 | -0.02 | 0.3 | NO |
217 | PSME4 | PSME4 | PSME4 | 10085 | -0.02 | 0.3 | NO |
218 | EIF4G1 | EIF4G1 | EIF4G1 | 10099 | -0.02 | 0.3 | NO |
219 | PSMA5 | PSMA5 | PSMA5 | 10336 | -0.023 | 0.29 | NO |
220 | PSMC5 | PSMC5 | PSMC5 | 10338 | -0.023 | 0.3 | NO |
221 | PSMD14 | PSMD14 | PSMD14 | 10343 | -0.024 | 0.3 | NO |
222 | PSMD3 | PSMD3 | PSMD3 | 10357 | -0.024 | 0.3 | NO |
223 | PSMD6 | PSMD6 | PSMD6 | 10381 | -0.024 | 0.3 | NO |
224 | PSMA7 | PSMA7 | PSMA7 | 10387 | -0.024 | 0.3 | NO |
225 | PSMD13 | PSMD13 | PSMD13 | 10417 | -0.025 | 0.3 | NO |
226 | NUP188 | NUP188 | NUP188 | 10436 | -0.025 | 0.3 | NO |
227 | PSMC2 | PSMC2 | PSMC2 | 10726 | -0.03 | 0.29 | NO |
228 | PSMB10 | PSMB10 | PSMB10 | 10739 | -0.03 | 0.29 | NO |
229 | PSMD1 | PSMD1 | PSMD1 | 10787 | -0.031 | 0.29 | NO |
230 | PSMB8 | PSMB8 | PSMB8 | 10862 | -0.032 | 0.29 | NO |
231 | PSMB6 | PSMB6 | PSMB6 | 10893 | -0.032 | 0.29 | NO |
232 | RPL26L1 | RPL26L1 | RPL26L1 | 11064 | -0.035 | 0.28 | NO |
233 | PATL1 | PATL1 | PATL1 | 11202 | -0.038 | 0.28 | NO |
234 | CNOT10 | CNOT10 | CNOT10 | 11323 | -0.04 | 0.27 | NO |
235 | NUP93 | NUP93 | NUP93 | 11363 | -0.041 | 0.28 | NO |
236 | PSMD11 | PSMD11 | PSMD11 | 11408 | -0.042 | 0.28 | NO |
237 | MAPK11 | MAPK11 | MAPK11 | 11478 | -0.043 | 0.28 | NO |
238 | PSMD9 | PSMD9 | PSMD9 | 11542 | -0.044 | 0.28 | NO |
239 | PSMD8 | PSMD8 | PSMD8 | 11743 | -0.048 | 0.27 | NO |
240 | PSMC4 | PSMC4 | PSMC4 | 11745 | -0.048 | 0.27 | NO |
241 | PSMB3 | PSMB3 | PSMB3 | 11762 | -0.048 | 0.28 | NO |
242 | RQCD1 | RQCD1 | RQCD1 | 11959 | -0.052 | 0.27 | NO |
243 | MAPKAPK2 | MAPKAPK2 | MAPKAPK2 | 12015 | -0.053 | 0.27 | NO |
244 | TNFSF13 | TNFSF13 | TNFSF13 | 12152 | -0.056 | 0.27 | NO |
245 | PSMC3 | PSMC3 | PSMC3 | 12312 | -0.059 | 0.26 | NO |
246 | TNKS1BP1 | TNKS1BP1 | TNKS1BP1 | 12420 | -0.062 | 0.26 | NO |
247 | PSMD2 | PSMD2 | PSMD2 | 12871 | -0.072 | 0.24 | NO |
248 | NUP210 | NUP210 | NUP210 | 13232 | -0.082 | 0.23 | NO |
249 | EXOSC1 | EXOSC1 | EXOSC1 | 13951 | -0.1 | 0.2 | NO |
250 | PSMB9 | PSMB9 | PSMB9 | 14243 | -0.11 | 0.19 | NO |
251 | ZFP36 | ZFP36 | ZFP36 | 14718 | -0.13 | 0.17 | NO |
252 | FAM153A | FAM153A | FAM153A | 15595 | -0.17 | 0.14 | NO |
Figure S61. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG LYSINE DEGRADATION.

Figure S62. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG LYSINE DEGRADATION, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S32. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | LSM11 | LSM11 | LSM11 | 1072 | 0.17 | -0.028 | YES |
2 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | -0.017 | YES |
3 | GTF2H2B | GTF2H2B | GTF2H2B | 1625 | 0.13 | -0.0051 | YES |
4 | HNRNPA1 | HNRNPA1 | HNRNPA1 | 1942 | 0.12 | -9e-05 | YES |
5 | NUP133 | NUP133 | NUP133 | 2383 | 0.1 | -0.0051 | YES |
6 | GTF2H2 | GTF2H2 | GTF2H2 | 2436 | 0.1 | 0.011 | YES |
7 | SNRPD1 | SNRPD1 | SNRPD1 | 2442 | 0.1 | 0.03 | YES |
8 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | 0.04 | YES |
9 | GTF2F2 | GTF2F2 | GTF2F2 | 2617 | 0.097 | 0.057 | YES |
10 | ZNF473 | ZNF473 | ZNF473 | 2872 | 0.09 | 0.06 | YES |
11 | RBMX | RBMX | RBMX | 3050 | 0.085 | 0.067 | YES |
12 | NUP153 | NUP153 | NUP153 | 3074 | 0.085 | 0.082 | YES |
13 | RBM5 | RBM5 | RBM5 | 3117 | 0.084 | 0.095 | YES |
14 | TPR | TPR | TPR | 3674 | 0.071 | 0.077 | YES |
15 | HNRNPH1 | HNRNPH1 | HNRNPH1 | 3678 | 0.071 | 0.091 | YES |
16 | POLR2K | POLR2K | POLR2K | 3695 | 0.07 | 0.1 | YES |
17 | HNRNPA3 | HNRNPA3 | HNRNPA3 | 4031 | 0.064 | 0.097 | YES |
18 | MNAT1 | MNAT1 | MNAT1 | 4042 | 0.064 | 0.11 | YES |
19 | GTF2H1 | GTF2H1 | GTF2H1 | 4232 | 0.06 | 0.11 | YES |
20 | NUP43 | NUP43 | NUP43 | 4261 | 0.06 | 0.12 | YES |
21 | NUP35 | NUP35 | NUP35 | 4302 | 0.059 | 0.13 | YES |
22 | METTL3 | METTL3 | METTL3 | 4307 | 0.059 | 0.14 | YES |
23 | SEH1L | SEH1L | SEH1L | 4336 | 0.058 | 0.15 | YES |
24 | ADARB1 | ADARB1 | ADARB1 | 4356 | 0.058 | 0.16 | YES |
25 | CSTF3 | CSTF3 | CSTF3 | 4360 | 0.058 | 0.17 | YES |
26 | CPSF1 | CPSF1 | CPSF1 | 4397 | 0.057 | 0.18 | YES |
27 | HNRNPA0 | HNRNPA0 | HNRNPA0 | 4409 | 0.057 | 0.19 | YES |
28 | SNRNP70 | SNRNP70 | SNRNP70 | 4438 | 0.056 | 0.2 | YES |
29 | NUPL1 | NUPL1 | NUPL1 | 4485 | 0.056 | 0.21 | YES |
30 | DHX9 | DHX9 | DHX9 | 4582 | 0.054 | 0.21 | YES |
31 | ERCC2 | ERCC2 | ERCC2 | 4644 | 0.053 | 0.22 | YES |
32 | NUP107 | NUP107 | NUP107 | 4673 | 0.053 | 0.23 | YES |
33 | LSM2 | LSM2 | LSM2 | 4703 | 0.052 | 0.23 | YES |
34 | PCF11 | PCF11 | PCF11 | 4728 | 0.052 | 0.24 | YES |
35 | NUP54 | NUP54 | NUP54 | 4772 | 0.051 | 0.25 | YES |
36 | NFX1 | NFX1 | NFX1 | 4859 | 0.05 | 0.26 | YES |
37 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.26 | YES |
38 | SMC1A | SMC1A | SMC1A | 5007 | 0.048 | 0.26 | YES |
39 | CDK7 | CDK7 | CDK7 | 5105 | 0.046 | 0.27 | YES |
40 | HNRNPC | HNRNPC | HNRNPC | 5125 | 0.046 | 0.28 | YES |
41 | RNMT | RNMT | RNMT | 5197 | 0.045 | 0.28 | YES |
42 | PAPOLA | PAPOLA | PAPOLA | 5248 | 0.044 | 0.29 | YES |
43 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.29 | YES |
44 | PABPN1 | PABPN1 | PABPN1 | 5387 | 0.042 | 0.3 | YES |
45 | HNRNPM | HNRNPM | HNRNPM | 5392 | 0.042 | 0.3 | YES |
46 | CCAR1 | CCAR1 | CCAR1 | 5394 | 0.042 | 0.31 | YES |
47 | PCBP2 | PCBP2 | PCBP2 | 5401 | 0.042 | 0.32 | YES |
48 | NUDT21 | NUDT21 | NUDT21 | 5407 | 0.042 | 0.33 | YES |
49 | HNRNPU | HNRNPU | HNRNPU | 5423 | 0.042 | 0.33 | YES |
50 | HNRNPD | HNRNPD | HNRNPD | 5462 | 0.041 | 0.34 | YES |
51 | HNRNPA2B1 | HNRNPA2B1 | HNRNPA2B1 | 5503 | 0.041 | 0.34 | YES |
52 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.34 | YES |
53 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.35 | YES |
54 | SF3B1 | SF3B1 | SF3B1 | 5735 | 0.038 | 0.35 | YES |
55 | GTF2H4 | GTF2H4 | GTF2H4 | 5810 | 0.037 | 0.36 | YES |
56 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.36 | YES |
57 | HNRNPR | HNRNPR | HNRNPR | 5834 | 0.037 | 0.37 | YES |
58 | HNRNPK | HNRNPK | HNRNPK | 5861 | 0.036 | 0.38 | YES |
59 | EIF4E | EIF4E | EIF4E | 5918 | 0.035 | 0.38 | YES |
60 | FUS | FUS | FUS | 5948 | 0.035 | 0.38 | YES |
61 | SNRPB2 | SNRPB2 | SNRPB2 | 6105 | 0.033 | 0.38 | YES |
62 | NUPL2 | NUPL2 | NUPL2 | 6137 | 0.032 | 0.39 | YES |
63 | NUP88 | NUP88 | NUP88 | 6196 | 0.032 | 0.39 | YES |
64 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.39 | YES |
65 | SF3B14 | SF3B14 | SF3B14 | 6241 | 0.031 | 0.4 | YES |
66 | AAAS | AAAS | AAAS | 6275 | 0.03 | 0.4 | YES |
67 | SLBP | SLBP | SLBP | 6289 | 0.03 | 0.41 | YES |
68 | GTF2H3 | GTF2H3 | GTF2H3 | 6374 | 0.029 | 0.41 | YES |
69 | SNRPD2 | SNRPD2 | SNRPD2 | 6381 | 0.029 | 0.41 | YES |
70 | NXF1 | NXF1 | NXF1 | 6481 | 0.028 | 0.41 | YES |
71 | CCNH | CCNH | CCNH | 6492 | 0.028 | 0.42 | YES |
72 | SF3A3 | SF3A3 | SF3A3 | 6493 | 0.028 | 0.42 | YES |
73 | CSTF1 | CSTF1 | CSTF1 | 6511 | 0.027 | 0.43 | YES |
74 | SF3B3 | SF3B3 | SF3B3 | 6580 | 0.026 | 0.43 | YES |
75 | YBX1 | YBX1 | YBX1 | 6612 | 0.026 | 0.43 | YES |
76 | SNRPA1 | SNRPA1 | SNRPA1 | 6633 | 0.026 | 0.44 | YES |
77 | RNGTT | RNGTT | RNGTT | 6917 | 0.022 | 0.42 | YES |
78 | NUP85 | NUP85 | NUP85 | 6959 | 0.021 | 0.42 | YES |
79 | HNRNPH2 | HNRNPH2 | HNRNPH2 | 6979 | 0.021 | 0.43 | YES |
80 | POLR2B | POLR2B | POLR2B | 6982 | 0.021 | 0.43 | YES |
81 | HNRNPF | HNRNPF | HNRNPF | 7043 | 0.02 | 0.43 | YES |
82 | HNRNPUL1 | HNRNPUL1 | HNRNPUL1 | 7214 | 0.018 | 0.43 | YES |
83 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.42 | YES |
84 | RAE1 | RAE1 | RAE1 | 7320 | 0.016 | 0.43 | YES |
85 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.43 | YES |
86 | U2AF1 | U2AF1 | U2AF1 | 7354 | 0.016 | 0.43 | YES |
87 | NUP37 | NUP37 | NUP37 | 7365 | 0.016 | 0.43 | YES |
88 | HNRNPL | HNRNPL | HNRNPL | 7390 | 0.016 | 0.44 | YES |
89 | SNRPA | SNRPA | SNRPA | 7491 | 0.014 | 0.43 | YES |
90 | SNRNP200 | SNRNP200 | SNRNP200 | 7534 | 0.014 | 0.43 | YES |
91 | POLR2G | POLR2G | POLR2G | 7554 | 0.014 | 0.43 | YES |
92 | PRPF4 | PRPF4 | PRPF4 | 7620 | 0.013 | 0.43 | YES |
93 | PTBP1 | PTBP1 | PTBP1 | 7662 | 0.012 | 0.43 | YES |
94 | PRPF6 | PRPF6 | PRPF6 | 7685 | 0.012 | 0.43 | YES |
95 | POLR2D | POLR2D | POLR2D | 7717 | 0.012 | 0.44 | YES |
96 | CDC40 | CDC40 | CDC40 | 7784 | 0.011 | 0.43 | YES |
97 | SF3A2 | SF3A2 | SF3A2 | 7798 | 0.01 | 0.43 | YES |
98 | NHP2L1 | NHP2L1 | NHP2L1 | 7813 | 0.01 | 0.44 | YES |
99 | CPSF3 | CPSF3 | CPSF3 | 7830 | 0.01 | 0.44 | YES |
100 | CPSF7 | CPSF7 | CPSF7 | 7905 | 0.0092 | 0.43 | NO |
101 | POM121 | POM121 | POM121 | 7942 | 0.0088 | 0.43 | NO |
102 | CLP1 | CLP1 | CLP1 | 8068 | 0.007 | 0.43 | NO |
103 | DNAJC8 | DNAJC8 | DNAJC8 | 8124 | 0.0065 | 0.43 | NO |
104 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.42 | NO |
105 | ADAR | ADAR | ADAR | 8198 | 0.0054 | 0.42 | NO |
106 | RANBP2 | RANBP2 | RANBP2 | 8229 | 0.0051 | 0.42 | NO |
107 | U2AF2 | U2AF2 | U2AF2 | 8289 | 0.0044 | 0.42 | NO |
108 | PRPF8 | PRPF8 | PRPF8 | 8292 | 0.0044 | 0.42 | NO |
109 | DDX23 | DDX23 | DDX23 | 8310 | 0.0042 | 0.42 | NO |
110 | ERCC3 | ERCC3 | ERCC3 | 8344 | 0.0038 | 0.42 | NO |
111 | GTF2F1 | GTF2F1 | GTF2F1 | 8371 | 0.0034 | 0.42 | NO |
112 | EFTUD2 | EFTUD2 | EFTUD2 | 8402 | 0.003 | 0.42 | NO |
113 | NUP62 | NUP62 | NUP62 | 8411 | 0.0028 | 0.42 | NO |
114 | NUP50 | NUP50 | NUP50 | 8461 | 0.0023 | 0.42 | NO |
115 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.41 | NO |
116 | CPSF2 | CPSF2 | CPSF2 | 8739 | -0.0015 | 0.4 | NO |
117 | TXNL4A | TXNL4A | TXNL4A | 8755 | -0.0018 | 0.4 | NO |
118 | SUPT5H | SUPT5H | SUPT5H | 8819 | -0.0024 | 0.4 | NO |
119 | POLR2E | POLR2E | POLR2E | 8851 | -0.0029 | 0.4 | NO |
120 | SRRM1 | SRRM1 | SRRM1 | 8870 | -0.003 | 0.4 | NO |
121 | SF3B4 | SF3B4 | SF3B4 | 8872 | -0.003 | 0.4 | NO |
122 | SNRNP40 | SNRNP40 | SNRNP40 | 8967 | -0.0041 | 0.39 | NO |
123 | POLR2I | POLR2I | POLR2I | 9140 | -0.0063 | 0.38 | NO |
124 | POLR2F | POLR2F | POLR2F | 9330 | -0.0091 | 0.38 | NO |
125 | THOC4 | THOC4 | THOC4 | 9367 | -0.0097 | 0.38 | NO |
126 | NUP155 | NUP155 | NUP155 | 9409 | -0.01 | 0.38 | NO |
127 | DHX38 | DHX38 | DHX38 | 9443 | -0.011 | 0.38 | NO |
128 | CSTF2 | CSTF2 | CSTF2 | 9466 | -0.011 | 0.38 | NO |
129 | SF3A1 | SF3A1 | SF3A1 | 9630 | -0.013 | 0.37 | NO |
130 | NUP205 | NUP205 | NUP205 | 9681 | -0.014 | 0.37 | NO |
131 | SF3B2 | SF3B2 | SF3B2 | 9729 | -0.015 | 0.37 | NO |
132 | SF3B5 | SF3B5 | SF3B5 | 9742 | -0.015 | 0.37 | NO |
133 | PHF5A | PHF5A | PHF5A | 9914 | -0.018 | 0.36 | NO |
134 | POLR2H | POLR2H | POLR2H | 9915 | -0.018 | 0.37 | NO |
135 | PCBP1 | PCBP1 | PCBP1 | 10196 | -0.022 | 0.36 | NO |
136 | CD2BP2 | CD2BP2 | CD2BP2 | 10411 | -0.025 | 0.35 | NO |
137 | NUP188 | NUP188 | NUP188 | 10436 | -0.025 | 0.35 | NO |
138 | POLR2L | POLR2L | POLR2L | 10911 | -0.032 | 0.33 | NO |
139 | POLR2J | POLR2J | POLR2J | 11204 | -0.038 | 0.32 | NO |
140 | NUP93 | NUP93 | NUP93 | 11363 | -0.041 | 0.32 | NO |
141 | POLR2C | POLR2C | POLR2C | 11828 | -0.05 | 0.31 | NO |
142 | POLR2A | POLR2A | POLR2A | 12478 | -0.063 | 0.28 | NO |
143 | LSM10 | LSM10 | LSM10 | 12758 | -0.069 | 0.28 | NO |
144 | NUP210 | NUP210 | NUP210 | 13232 | -0.082 | 0.27 | NO |
Figure S63. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG RIBOSOME.

Figure S64. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG RIBOSOME, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S33. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | RPL36A | RPL36A | RPL36A | 919 | 0.18 | -0.034 | YES |
2 | RPS27 | RPS27 | RPS27 | 1011 | 0.17 | -0.022 | YES |
3 | RPL7 | RPL7 | RPL7 | 1214 | 0.16 | -0.018 | YES |
4 | DCP2 | DCP2 | DCP2 | 1362 | 0.15 | -0.012 | YES |
5 | PRKCA | PRKCA | PRKCA | 1378 | 0.15 | 0.001 | YES |
6 | RPL21 | RPL21 | RPL21 | 1606 | 0.13 | 0.0013 | YES |
7 | RPL9 | RPL9 | RPL9 | 1630 | 0.13 | 0.013 | YES |
8 | RPS3A | RPS3A | RPS3A | 1684 | 0.13 | 0.022 | YES |
9 | RPL39 | RPL39 | RPL39 | 1806 | 0.12 | 0.028 | YES |
10 | PABPC1 | PABPC1 | PABPC1 | 1834 | 0.12 | 0.038 | YES |
11 | EXOSC8 | EXOSC8 | EXOSC8 | 1968 | 0.12 | 0.042 | YES |
12 | RPS7 | RPS7 | RPS7 | 2333 | 0.1 | 0.032 | YES |
13 | RPS12 | RPS12 | RPS12 | 2433 | 0.1 | 0.036 | YES |
14 | PAIP1 | PAIP1 | PAIP1 | 2524 | 0.099 | 0.041 | YES |
15 | RPS18 | RPS18 | RPS18 | 2533 | 0.099 | 0.05 | YES |
16 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | 0.055 | YES |
17 | RPSAP9 | RPSAP9 | RPSAP9 | 2706 | 0.094 | 0.058 | YES |
18 | RPS23 | RPS23 | RPS23 | 2738 | 0.093 | 0.066 | YES |
19 | RPL30 | RPL30 | RPL30 | 2787 | 0.092 | 0.072 | YES |
20 | EIF4B | EIF4B | EIF4B | 2852 | 0.09 | 0.077 | YES |
21 | RPS10 | RPS10 | RPS10 | 2869 | 0.09 | 0.085 | YES |
22 | RPL17 | RPL17 | RPL17 | 2876 | 0.09 | 0.093 | YES |
23 | RPL22 | RPL22 | RPL22 | 2917 | 0.088 | 0.1 | YES |
24 | RPS20 | RPS20 | RPS20 | 2996 | 0.086 | 0.1 | YES |
25 | RPL26 | RPL26 | RPL26 | 3006 | 0.086 | 0.11 | YES |
26 | RPL8 | RPL8 | RPL8 | 3011 | 0.086 | 0.12 | YES |
27 | RPL10A | RPL10A | RPL10A | 3034 | 0.085 | 0.13 | YES |
28 | RPL34 | RPL34 | RPL34 | 3039 | 0.085 | 0.13 | YES |
29 | DIS3 | DIS3 | DIS3 | 3045 | 0.085 | 0.14 | YES |
30 | UPF3A | UPF3A | UPF3A | 3049 | 0.085 | 0.15 | YES |
31 | CNOT6 | CNOT6 | CNOT6 | 3056 | 0.085 | 0.16 | YES |
32 | RPL23 | RPL23 | RPL23 | 3186 | 0.082 | 0.16 | YES |
33 | RPL5 | RPL5 | RPL5 | 3230 | 0.081 | 0.16 | YES |
34 | RPL6 | RPL6 | RPL6 | 3280 | 0.08 | 0.17 | YES |
35 | RPS8 | RPS8 | RPS8 | 3378 | 0.078 | 0.17 | YES |
36 | RPS6 | RPS6 | RPS6 | 3504 | 0.075 | 0.17 | YES |
37 | RPS26 | RPS26 | RPS26 | 3519 | 0.074 | 0.18 | YES |
38 | RPS24 | RPS24 | RPS24 | 3538 | 0.074 | 0.18 | YES |
39 | ANP32A | ANP32A | ANP32A | 3645 | 0.071 | 0.18 | YES |
40 | EXOSC2 | EXOSC2 | EXOSC2 | 3683 | 0.07 | 0.19 | YES |
41 | EIF4A2 | EIF4A2 | EIF4A2 | 3688 | 0.07 | 0.2 | YES |
42 | RPL13A | RPL13A | RPL13A | 3735 | 0.069 | 0.2 | YES |
43 | RPS17 | RPS17 | RPS17 | 3826 | 0.068 | 0.2 | YES |
44 | RPS4X | RPS4X | RPS4X | 3829 | 0.068 | 0.21 | YES |
45 | RPL4 | RPL4 | RPL4 | 3835 | 0.067 | 0.22 | YES |
46 | GSPT2 | GSPT2 | GSPT2 | 3851 | 0.067 | 0.22 | YES |
47 | UPF2 | UPF2 | UPF2 | 3877 | 0.067 | 0.22 | YES |
48 | RPL3 | RPL3 | RPL3 | 3889 | 0.066 | 0.23 | YES |
49 | LSM6 | LSM6 | LSM6 | 3944 | 0.066 | 0.23 | YES |
50 | RPL31 | RPL31 | RPL31 | 3949 | 0.065 | 0.24 | YES |
51 | RPL12 | RPL12 | RPL12 | 3978 | 0.065 | 0.24 | YES |
52 | RPS3 | RPS3 | RPS3 | 3990 | 0.065 | 0.25 | YES |
53 | PSMA8 | PSMA8 | PSMA8 | 4010 | 0.064 | 0.26 | YES |
54 | RPL38 | RPL38 | RPL38 | 4039 | 0.064 | 0.26 | YES |
55 | RPS14 | RPS14 | RPS14 | 4056 | 0.064 | 0.27 | YES |
56 | RPS15A | RPS15A | RPS15A | 4070 | 0.063 | 0.27 | YES |
57 | RPS19 | RPS19 | RPS19 | 4166 | 0.061 | 0.27 | YES |
58 | RPS27A | RPS27A | RPS27A | 4186 | 0.061 | 0.28 | YES |
59 | RPS21 | RPS21 | RPS21 | 4216 | 0.061 | 0.28 | YES |
60 | RPS29 | RPS29 | RPS29 | 4284 | 0.059 | 0.28 | YES |
61 | EXOSC4 | EXOSC4 | EXOSC4 | 4314 | 0.059 | 0.29 | YES |
62 | RPS5 | RPS5 | RPS5 | 4329 | 0.058 | 0.29 | YES |
63 | PSMC6 | PSMC6 | PSMC6 | 4339 | 0.058 | 0.3 | YES |
64 | EXOSC5 | EXOSC5 | EXOSC5 | 4347 | 0.058 | 0.3 | YES |
65 | RPL7A | RPL7A | RPL7A | 4365 | 0.058 | 0.31 | YES |
66 | MAPK14 | MAPK14 | MAPK14 | 4371 | 0.058 | 0.31 | YES |
67 | RPL23A | RPL23A | RPL23A | 4386 | 0.057 | 0.32 | YES |
68 | DCP1B | DCP1B | DCP1B | 4425 | 0.057 | 0.32 | YES |
69 | RPL14 | RPL14 | RPL14 | 4551 | 0.055 | 0.32 | YES |
70 | KHSRP | KHSRP | KHSRP | 4578 | 0.054 | 0.32 | YES |
71 | PPP2R2A | PPP2R2A | PPP2R2A | 4603 | 0.054 | 0.33 | YES |
72 | RPL37 | RPL37 | RPL37 | 4658 | 0.053 | 0.33 | YES |
73 | RPL24 | RPL24 | RPL24 | 4701 | 0.053 | 0.33 | YES |
74 | LSM2 | LSM2 | LSM2 | 4703 | 0.052 | 0.34 | YES |
75 | RPL35A | RPL35A | RPL35A | 4720 | 0.052 | 0.34 | YES |
76 | RPS16 | RPS16 | RPS16 | 4737 | 0.052 | 0.34 | YES |
77 | EXOSC3 | EXOSC3 | EXOSC3 | 4751 | 0.052 | 0.35 | YES |
78 | RPL29 | RPL29 | RPL29 | 4763 | 0.052 | 0.35 | YES |
79 | RPS25 | RPS25 | RPS25 | 4769 | 0.051 | 0.36 | YES |
80 | CNOT8 | CNOT8 | CNOT8 | 4835 | 0.05 | 0.36 | YES |
81 | RPL18 | RPL18 | RPL18 | 4906 | 0.049 | 0.36 | YES |
82 | RPL36 | RPL36 | RPL36 | 4907 | 0.049 | 0.36 | YES |
83 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.37 | YES |
84 | RPL37A | RPL37A | RPL37A | 4981 | 0.048 | 0.37 | YES |
85 | XPO1 | XPO1 | XPO1 | 4996 | 0.048 | 0.37 | YES |
86 | PSMD10 | PSMD10 | PSMD10 | 4997 | 0.048 | 0.38 | YES |
87 | RPL32 | RPL32 | RPL32 | 5021 | 0.048 | 0.38 | YES |
88 | CNOT7 | CNOT7 | CNOT7 | 5039 | 0.047 | 0.38 | YES |
89 | RPL11 | RPL11 | RPL11 | 5057 | 0.047 | 0.39 | YES |
90 | RPS13 | RPS13 | RPS13 | 5119 | 0.046 | 0.39 | YES |
91 | RPL19 | RPL19 | RPL19 | 5136 | 0.046 | 0.39 | YES |
92 | PSMB4 | PSMB4 | PSMB4 | 5237 | 0.045 | 0.39 | YES |
93 | RPLP1 | RPLP1 | RPLP1 | 5252 | 0.044 | 0.4 | YES |
94 | RPS11 | RPS11 | RPS11 | 5255 | 0.044 | 0.4 | YES |
95 | RPL18A | RPL18A | RPL18A | 5283 | 0.044 | 0.4 | YES |
96 | RPS2 | RPS2 | RPS2 | 5290 | 0.044 | 0.41 | YES |
97 | RPLP0 | RPLP0 | RPLP0 | 5343 | 0.043 | 0.41 | YES |
98 | RPL15 | RPL15 | RPL15 | 5370 | 0.043 | 0.41 | YES |
99 | HNRNPD | HNRNPD | HNRNPD | 5462 | 0.041 | 0.41 | YES |
100 | RPS9 | RPS9 | RPS9 | 5501 | 0.041 | 0.41 | YES |
101 | RPL13 | RPL13 | RPL13 | 5526 | 0.04 | 0.41 | YES |
102 | DDX6 | DDX6 | DDX6 | 5589 | 0.04 | 0.41 | YES |
103 | DCP1A | DCP1A | DCP1A | 5691 | 0.038 | 0.41 | YES |
104 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.42 | YES |
105 | RPL27 | RPL27 | RPL27 | 5712 | 0.038 | 0.42 | YES |
106 | HSPA1B | HSPA1B | HSPA1B | 5721 | 0.038 | 0.42 | YES |
107 | RPL27A | RPL27A | RPL27A | 5771 | 0.037 | 0.42 | YES |
108 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.42 | YES |
109 | RPS15 | RPS15 | RPS15 | 5875 | 0.036 | 0.42 | YES |
110 | SMG5 | SMG5 | SMG5 | 5892 | 0.036 | 0.43 | YES |
111 | EIF4E | EIF4E | EIF4E | 5918 | 0.035 | 0.43 | YES |
112 | RPL35 | RPL35 | RPL35 | 5939 | 0.035 | 0.43 | YES |
113 | LSM5 | LSM5 | LSM5 | 5945 | 0.035 | 0.43 | YES |
114 | RPL41 | RPL41 | RPL41 | 5947 | 0.035 | 0.44 | YES |
115 | ETF1 | ETF1 | ETF1 | 6067 | 0.033 | 0.43 | NO |
116 | RPS28 | RPS28 | RPS28 | 6078 | 0.033 | 0.44 | NO |
117 | PSMA4 | PSMA4 | PSMA4 | 6160 | 0.032 | 0.43 | NO |
118 | RPSA | RPSA | RPSA | 6191 | 0.032 | 0.44 | NO |
119 | ZFP36L1 | ZFP36L1 | ZFP36L1 | 6326 | 0.03 | 0.43 | NO |
120 | EXOSC7 | EXOSC7 | EXOSC7 | 6431 | 0.028 | 0.43 | NO |
121 | RPL10 | RPL10 | RPL10 | 6540 | 0.027 | 0.42 | NO |
122 | SMG7 | SMG7 | SMG7 | 6721 | 0.024 | 0.42 | NO |
123 | CNOT2 | CNOT2 | CNOT2 | 6736 | 0.024 | 0.42 | NO |
124 | PSMD5 | PSMD5 | PSMD5 | 6789 | 0.024 | 0.42 | NO |
125 | PPP2CA | PPP2CA | PPP2CA | 6970 | 0.021 | 0.41 | NO |
126 | HSPA8 | HSPA8 | HSPA8 | 6986 | 0.021 | 0.41 | NO |
127 | EIF4A3 | EIF4A3 | EIF4A3 | 7000 | 0.021 | 0.41 | NO |
128 | ELAVL1 | ELAVL1 | ELAVL1 | 7068 | 0.02 | 0.41 | NO |
129 | PSMF1 | PSMF1 | PSMF1 | 7099 | 0.02 | 0.41 | NO |
130 | TNPO1 | TNPO1 | TNPO1 | 7122 | 0.019 | 0.41 | NO |
131 | PSMA3 | PSMA3 | PSMA3 | 7222 | 0.018 | 0.41 | NO |
132 | PSMB7 | PSMB7 | PSMB7 | 7234 | 0.018 | 0.41 | NO |
133 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.4 | NO |
134 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.41 | NO |
135 | YWHAZ | YWHAZ | YWHAZ | 7397 | 0.015 | 0.4 | NO |
136 | PPP2R1A | PPP2R1A | PPP2R1A | 7427 | 0.015 | 0.4 | NO |
137 | PSMD4 | PSMD4 | PSMD4 | 7536 | 0.014 | 0.4 | NO |
138 | PSMA1 | PSMA1 | PSMA1 | 7581 | 0.013 | 0.4 | NO |
139 | RPLP2 | RPLP2 | RPLP2 | 7610 | 0.013 | 0.4 | NO |
140 | RPL28 | RPL28 | RPL28 | 7615 | 0.013 | 0.4 | NO |
141 | LSM4 | LSM4 | LSM4 | 7726 | 0.012 | 0.39 | NO |
142 | XRN1 | XRN1 | XRN1 | 7735 | 0.011 | 0.39 | NO |
143 | PARN | PARN | PARN | 7893 | 0.0093 | 0.39 | NO |
144 | EIF4A1 | EIF4A1 | EIF4A1 | 7945 | 0.0087 | 0.38 | NO |
145 | PRKCD | PRKCD | PRKCD | 7965 | 0.0086 | 0.38 | NO |
146 | SMG1 | SMG1 | SMG1 | 8134 | 0.0063 | 0.38 | NO |
147 | CASC3 | CASC3 | CASC3 | 8151 | 0.006 | 0.38 | NO |
148 | EDC3 | EDC3 | EDC3 | 8162 | 0.0058 | 0.38 | NO |
149 | CNOT4 | CNOT4 | CNOT4 | 8167 | 0.0058 | 0.38 | NO |
150 | SMG6 | SMG6 | SMG6 | 8171 | 0.0057 | 0.38 | NO |
151 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.38 | NO |
152 | UBA52 | UBA52 | UBA52 | 8183 | 0.0056 | 0.38 | NO |
153 | HSPB1 | HSPB1 | HSPB1 | 8305 | 0.0043 | 0.37 | NO |
154 | PSMC1 | PSMC1 | PSMC1 | 8484 | 0.002 | 0.36 | NO |
155 | PSMA6 | PSMA6 | PSMA6 | 8490 | 0.002 | 0.36 | NO |
156 | PSMB1 | PSMB1 | PSMB1 | 8613 | 0.00026 | 0.35 | NO |
157 | PSMB5 | PSMB5 | PSMB5 | 8669 | -0.00052 | 0.35 | NO |
158 | FAU | FAU | FAU | 8710 | -0.0012 | 0.35 | NO |
159 | PSMA2 | PSMA2 | PSMA2 | 8764 | -0.0018 | 0.34 | NO |
160 | LSM3 | LSM3 | LSM3 | 8956 | -0.0039 | 0.34 | NO |
161 | AKT1 | AKT1 | AKT1 | 9125 | -0.0062 | 0.33 | NO |
162 | EDC4 | EDC4 | EDC4 | 9130 | -0.0063 | 0.33 | NO |
163 | CNOT3 | CNOT3 | CNOT3 | 9131 | -0.0063 | 0.33 | NO |
164 | EXOSC9 | EXOSC9 | EXOSC9 | 9150 | -0.0064 | 0.33 | NO |
165 | C2orf29 | C2orf29 | C2orf29 | 9190 | -0.007 | 0.32 | NO |
166 | RPS4Y1 | RPS4Y1 | RPS4Y1 | 9221 | -0.0074 | 0.32 | NO |
167 | LSM1 | LSM1 | LSM1 | 9285 | -0.0085 | 0.32 | NO |
168 | YWHAB | YWHAB | YWHAB | 9333 | -0.0091 | 0.32 | NO |
169 | PSMD12 | PSMD12 | PSMD12 | 9353 | -0.0095 | 0.32 | NO |
170 | PSMB2 | PSMB2 | PSMB2 | 9465 | -0.011 | 0.31 | NO |
171 | PSME1 | PSME1 | PSME1 | 9570 | -0.012 | 0.31 | NO |
172 | DCPS | DCPS | DCPS | 9655 | -0.014 | 0.31 | NO |
173 | EXOSC6 | EXOSC6 | EXOSC6 | 9797 | -0.016 | 0.3 | NO |
174 | PSME2 | PSME2 | PSME2 | 10052 | -0.019 | 0.29 | NO |
175 | PSMD7 | PSMD7 | PSMD7 | 10056 | -0.02 | 0.29 | NO |
176 | PSME4 | PSME4 | PSME4 | 10085 | -0.02 | 0.29 | NO |
177 | EIF4G1 | EIF4G1 | EIF4G1 | 10099 | -0.02 | 0.29 | NO |
178 | PSMA5 | PSMA5 | PSMA5 | 10336 | -0.023 | 0.28 | NO |
179 | PSMC5 | PSMC5 | PSMC5 | 10338 | -0.023 | 0.28 | NO |
180 | PSMD14 | PSMD14 | PSMD14 | 10343 | -0.024 | 0.28 | NO |
181 | PSMD3 | PSMD3 | PSMD3 | 10357 | -0.024 | 0.29 | NO |
182 | PSMD6 | PSMD6 | PSMD6 | 10381 | -0.024 | 0.29 | NO |
183 | PSMA7 | PSMA7 | PSMA7 | 10387 | -0.024 | 0.29 | NO |
184 | PSMD13 | PSMD13 | PSMD13 | 10417 | -0.025 | 0.29 | NO |
185 | PSMC2 | PSMC2 | PSMC2 | 10726 | -0.03 | 0.28 | NO |
186 | PSMB10 | PSMB10 | PSMB10 | 10739 | -0.03 | 0.28 | NO |
187 | PSMD1 | PSMD1 | PSMD1 | 10787 | -0.031 | 0.28 | NO |
188 | PSMB8 | PSMB8 | PSMB8 | 10862 | -0.032 | 0.28 | NO |
189 | PSMB6 | PSMB6 | PSMB6 | 10893 | -0.032 | 0.28 | NO |
190 | RPL26L1 | RPL26L1 | RPL26L1 | 11064 | -0.035 | 0.27 | NO |
191 | PATL1 | PATL1 | PATL1 | 11202 | -0.038 | 0.27 | NO |
192 | CNOT10 | CNOT10 | CNOT10 | 11323 | -0.04 | 0.26 | NO |
193 | PSMD11 | PSMD11 | PSMD11 | 11408 | -0.042 | 0.26 | NO |
194 | MAPK11 | MAPK11 | MAPK11 | 11478 | -0.043 | 0.26 | NO |
195 | PSMD9 | PSMD9 | PSMD9 | 11542 | -0.044 | 0.26 | NO |
196 | PSMD8 | PSMD8 | PSMD8 | 11743 | -0.048 | 0.26 | NO |
197 | PSMC4 | PSMC4 | PSMC4 | 11745 | -0.048 | 0.26 | NO |
198 | PSMB3 | PSMB3 | PSMB3 | 11762 | -0.048 | 0.27 | NO |
199 | RQCD1 | RQCD1 | RQCD1 | 11959 | -0.052 | 0.26 | NO |
200 | MAPKAPK2 | MAPKAPK2 | MAPKAPK2 | 12015 | -0.053 | 0.26 | NO |
201 | TNFSF13 | TNFSF13 | TNFSF13 | 12152 | -0.056 | 0.26 | NO |
202 | PSMC3 | PSMC3 | PSMC3 | 12312 | -0.059 | 0.26 | NO |
203 | TNKS1BP1 | TNKS1BP1 | TNKS1BP1 | 12420 | -0.062 | 0.26 | NO |
204 | PSMD2 | PSMD2 | PSMD2 | 12871 | -0.072 | 0.24 | NO |
205 | EXOSC1 | EXOSC1 | EXOSC1 | 13951 | -0.1 | 0.19 | NO |
206 | PSMB9 | PSMB9 | PSMB9 | 14243 | -0.11 | 0.18 | NO |
207 | ZFP36 | ZFP36 | ZFP36 | 14718 | -0.13 | 0.17 | NO |
208 | FAM153A | FAM153A | FAM153A | 15595 | -0.17 | 0.14 | NO |
Figure S65. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG BASAL TRANSCRIPTION FACTORS.

Figure S66. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG BASAL TRANSCRIPTION FACTORS, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S34. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | -0.042 | YES |
2 | THOC1 | THOC1 | THOC1 | 1649 | 0.13 | -0.025 | YES |
3 | HSPA2 | HSPA2 | HSPA2 | 1790 | 0.13 | -0.0023 | YES |
4 | HNRNPA1 | HNRNPA1 | HNRNPA1 | 1942 | 0.12 | 0.018 | YES |
5 | HNRNPA1L2 | HNRNPA1L2 | HNRNPA1L2 | 1993 | 0.12 | 0.043 | YES |
6 | SNRPD1 | SNRPD1 | SNRPD1 | 2442 | 0.1 | 0.042 | YES |
7 | PUF60 | PUF60 | PUF60 | 2816 | 0.091 | 0.044 | YES |
8 | THOC2 | THOC2 | THOC2 | 2860 | 0.09 | 0.063 | YES |
9 | PRPF40B | PRPF40B | PRPF40B | 2871 | 0.09 | 0.084 | YES |
10 | PRPF38A | PRPF38A | PRPF38A | 3027 | 0.086 | 0.096 | YES |
11 | RBMX | RBMX | RBMX | 3050 | 0.085 | 0.12 | YES |
12 | TCERG1 | TCERG1 | TCERG1 | 3139 | 0.083 | 0.13 | YES |
13 | CDC5L | CDC5L | CDC5L | 3520 | 0.074 | 0.13 | YES |
14 | LSM6 | LSM6 | LSM6 | 3944 | 0.066 | 0.12 | YES |
15 | SNRPC | SNRPC | SNRPC | 3967 | 0.065 | 0.13 | YES |
16 | HNRNPA3 | HNRNPA3 | HNRNPA3 | 4031 | 0.064 | 0.14 | YES |
17 | PPIL1 | PPIL1 | PPIL1 | 4283 | 0.059 | 0.14 | YES |
18 | SNRNP70 | SNRNP70 | SNRNP70 | 4438 | 0.056 | 0.15 | YES |
19 | DHX15 | DHX15 | DHX15 | 4441 | 0.056 | 0.16 | YES |
20 | RBM25 | RBM25 | RBM25 | 4588 | 0.054 | 0.17 | YES |
21 | SNW1 | SNW1 | SNW1 | 4624 | 0.054 | 0.18 | YES |
22 | DDX46 | DDX46 | DDX46 | 4684 | 0.053 | 0.19 | YES |
23 | LSM2 | LSM2 | LSM2 | 4703 | 0.052 | 0.2 | YES |
24 | CRNKL1 | CRNKL1 | CRNKL1 | 4761 | 0.052 | 0.21 | YES |
25 | DDX42 | DDX42 | DDX42 | 4902 | 0.049 | 0.21 | YES |
26 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.22 | YES |
27 | TRA2B | TRA2B | TRA2B | 5012 | 0.048 | 0.23 | YES |
28 | HNRNPC | HNRNPC | HNRNPC | 5125 | 0.046 | 0.24 | YES |
29 | CWC15 | CWC15 | CWC15 | 5160 | 0.046 | 0.24 | YES |
30 | PRPF40A | PRPF40A | PRPF40A | 5187 | 0.045 | 0.25 | YES |
31 | PRPF3 | PRPF3 | PRPF3 | 5294 | 0.044 | 0.26 | YES |
32 | THOC3 | THOC3 | THOC3 | 5312 | 0.044 | 0.27 | YES |
33 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.28 | YES |
34 | HNRNPM | HNRNPM | HNRNPM | 5392 | 0.042 | 0.28 | YES |
35 | HNRNPU | HNRNPU | HNRNPU | 5423 | 0.042 | 0.29 | YES |
36 | PLRG1 | PLRG1 | PLRG1 | 5471 | 0.041 | 0.3 | YES |
37 | LSM7 | LSM7 | LSM7 | 5578 | 0.04 | 0.3 | YES |
38 | SLU7 | SLU7 | SLU7 | 5600 | 0.039 | 0.31 | YES |
39 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.32 | YES |
40 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.32 | YES |
41 | HSPA1B | HSPA1B | HSPA1B | 5721 | 0.038 | 0.33 | YES |
42 | SF3B1 | SF3B1 | SF3B1 | 5735 | 0.038 | 0.34 | YES |
43 | PRPF38B | PRPF38B | PRPF38B | 5762 | 0.037 | 0.35 | YES |
44 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.36 | YES |
45 | HNRNPK | HNRNPK | HNRNPK | 5861 | 0.036 | 0.36 | YES |
46 | ACIN1 | ACIN1 | ACIN1 | 5880 | 0.036 | 0.37 | YES |
47 | LSM5 | LSM5 | LSM5 | 5945 | 0.035 | 0.37 | YES |
48 | SNRPB2 | SNRPB2 | SNRPB2 | 6105 | 0.033 | 0.37 | YES |
49 | ZMAT2 | ZMAT2 | ZMAT2 | 6113 | 0.033 | 0.38 | YES |
50 | HSPA1A | HSPA1A | HSPA1A | 6156 | 0.032 | 0.39 | YES |
51 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.39 | YES |
52 | SF3B14 | SF3B14 | SF3B14 | 6241 | 0.031 | 0.4 | YES |
53 | SNRPD2 | SNRPD2 | SNRPD2 | 6381 | 0.029 | 0.4 | YES |
54 | SF3A3 | SF3A3 | SF3A3 | 6493 | 0.028 | 0.4 | YES |
55 | PRPF31 | PRPF31 | PRPF31 | 6559 | 0.026 | 0.4 | YES |
56 | SF3B3 | SF3B3 | SF3B3 | 6580 | 0.026 | 0.4 | YES |
57 | SNRPA1 | SNRPA1 | SNRPA1 | 6633 | 0.026 | 0.41 | YES |
58 | TRA2A | TRA2A | TRA2A | 6820 | 0.023 | 0.4 | YES |
59 | PPIH | PPIH | PPIH | 6898 | 0.022 | 0.4 | YES |
60 | PQBP1 | PQBP1 | PQBP1 | 6958 | 0.021 | 0.4 | YES |
61 | HSPA8 | HSPA8 | HSPA8 | 6986 | 0.021 | 0.41 | YES |
62 | EIF4A3 | EIF4A3 | EIF4A3 | 7000 | 0.021 | 0.41 | YES |
63 | MAGOHB | MAGOHB | MAGOHB | 7066 | 0.02 | 0.41 | YES |
64 | CHERP | CHERP | CHERP | 7082 | 0.02 | 0.42 | YES |
65 | SYF2 | SYF2 | SYF2 | 7083 | 0.02 | 0.42 | YES |
66 | PRPF19 | PRPF19 | PRPF19 | 7276 | 0.017 | 0.42 | NO |
67 | U2AF1 | U2AF1 | U2AF1 | 7354 | 0.016 | 0.42 | NO |
68 | SNRPA | SNRPA | SNRPA | 7491 | 0.014 | 0.41 | NO |
69 | DHX16 | DHX16 | DHX16 | 7502 | 0.014 | 0.41 | NO |
70 | SNRNP200 | SNRNP200 | SNRNP200 | 7534 | 0.014 | 0.42 | NO |
71 | PRPF4 | PRPF4 | PRPF4 | 7620 | 0.013 | 0.41 | NO |
72 | PRPF6 | PRPF6 | PRPF6 | 7685 | 0.012 | 0.41 | NO |
73 | LSM4 | LSM4 | LSM4 | 7726 | 0.012 | 0.41 | NO |
74 | CDC40 | CDC40 | CDC40 | 7784 | 0.011 | 0.41 | NO |
75 | XAB2 | XAB2 | XAB2 | 7788 | 0.011 | 0.42 | NO |
76 | SF3A2 | SF3A2 | SF3A2 | 7798 | 0.01 | 0.42 | NO |
77 | NHP2L1 | NHP2L1 | NHP2L1 | 7813 | 0.01 | 0.42 | NO |
78 | SMNDC1 | SMNDC1 | SMNDC1 | 7889 | 0.0093 | 0.42 | NO |
79 | DDX5 | DDX5 | DDX5 | 7927 | 0.009 | 0.42 | NO |
80 | RBM22 | RBM22 | RBM22 | 7936 | 0.0089 | 0.42 | NO |
81 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.41 | NO |
82 | PPIE | PPIE | PPIE | 8185 | 0.0055 | 0.41 | NO |
83 | HSPA1L | HSPA1L | HSPA1L | 8230 | 0.0051 | 0.41 | NO |
84 | U2AF2 | U2AF2 | U2AF2 | 8289 | 0.0044 | 0.4 | NO |
85 | PRPF8 | PRPF8 | PRPF8 | 8292 | 0.0044 | 0.41 | NO |
86 | DDX23 | DDX23 | DDX23 | 8310 | 0.0042 | 0.41 | NO |
87 | EFTUD2 | EFTUD2 | EFTUD2 | 8402 | 0.003 | 0.4 | NO |
88 | RBM17 | RBM17 | RBM17 | 8469 | 0.0022 | 0.4 | NO |
89 | WBP11 | WBP11 | WBP11 | 8492 | 0.0019 | 0.4 | NO |
90 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.39 | NO |
91 | TXNL4A | TXNL4A | TXNL4A | 8755 | -0.0018 | 0.38 | NO |
92 | CCDC12 | CCDC12 | CCDC12 | 8789 | -0.0021 | 0.38 | NO |
93 | SF3B4 | SF3B4 | SF3B4 | 8872 | -0.003 | 0.38 | NO |
94 | LSM3 | LSM3 | LSM3 | 8956 | -0.0039 | 0.38 | NO |
95 | SNRNP40 | SNRNP40 | SNRNP40 | 8967 | -0.0041 | 0.38 | NO |
96 | BCAS2 | BCAS2 | BCAS2 | 9099 | -0.0058 | 0.37 | NO |
97 | CTNNBL1 | CTNNBL1 | CTNNBL1 | 9324 | -0.009 | 0.36 | NO |
98 | THOC4 | THOC4 | THOC4 | 9367 | -0.0097 | 0.36 | NO |
99 | SNRNP27 | SNRNP27 | SNRNP27 | 9407 | -0.01 | 0.36 | NO |
100 | DHX38 | DHX38 | DHX38 | 9443 | -0.011 | 0.36 | NO |
101 | SF3A1 | SF3A1 | SF3A1 | 9630 | -0.013 | 0.35 | NO |
102 | BUD31 | BUD31 | BUD31 | 9686 | -0.014 | 0.35 | NO |
103 | SF3B2 | SF3B2 | SF3B2 | 9729 | -0.015 | 0.35 | NO |
104 | AQR | AQR | AQR | 9732 | -0.015 | 0.36 | NO |
105 | SF3B5 | SF3B5 | SF3B5 | 9742 | -0.015 | 0.36 | NO |
106 | SART1 | SART1 | SART1 | 9753 | -0.015 | 0.36 | NO |
107 | PHF5A | PHF5A | PHF5A | 9914 | -0.018 | 0.36 | NO |
108 | USP39 | USP39 | USP39 | 9934 | -0.018 | 0.36 | NO |
109 | PCBP1 | PCBP1 | PCBP1 | 10196 | -0.022 | 0.35 | NO |
110 | ISY1 | ISY1 | ISY1 | 10439 | -0.025 | 0.35 | NO |
111 | NAA38 | NAA38 | NAA38 | 10993 | -0.034 | 0.32 | NO |
112 | PRPF18 | PRPF18 | PRPF18 | 11450 | -0.042 | 0.31 | NO |
113 | DHX8 | DHX8 | DHX8 | 11765 | -0.048 | 0.3 | NO |
114 | HSPA6 | HSPA6 | HSPA6 | 16227 | -0.2 | 0.1 | NO |
Figure S67. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: KEGG SPLICEOSOME.

Figure S68. Get High-res Image For the top 5 core enriched genes in the pathway: KEGG SPLICEOSOME, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S35. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | RPL36A | RPL36A | RPL36A | 919 | 0.18 | -0.027 | YES |
2 | RPS27 | RPS27 | RPS27 | 1011 | 0.17 | -0.009 | YES |
3 | RPL7 | RPL7 | RPL7 | 1214 | 0.16 | 0.00068 | YES |
4 | RPL21 | RPL21 | RPL21 | 1606 | 0.13 | -0.0033 | YES |
5 | RPL9 | RPL9 | RPL9 | 1630 | 0.13 | 0.013 | YES |
6 | RPS3A | RPS3A | RPS3A | 1684 | 0.13 | 0.028 | YES |
7 | RPL39 | RPL39 | RPL39 | 1806 | 0.12 | 0.038 | YES |
8 | RPS7 | RPS7 | RPS7 | 2333 | 0.1 | 0.022 | YES |
9 | NUP133 | NUP133 | NUP133 | 2383 | 0.1 | 0.033 | YES |
10 | RPS12 | RPS12 | RPS12 | 2433 | 0.1 | 0.044 | YES |
11 | RPS18 | RPS18 | RPS18 | 2533 | 0.099 | 0.051 | YES |
12 | GTF2F2 | GTF2F2 | GTF2F2 | 2617 | 0.097 | 0.06 | YES |
13 | RPSAP9 | RPSAP9 | RPSAP9 | 2706 | 0.094 | 0.067 | YES |
14 | HSP90AA1 | HSP90AA1 | HSP90AA1 | 2713 | 0.094 | 0.079 | YES |
15 | RPS23 | RPS23 | RPS23 | 2738 | 0.093 | 0.09 | YES |
16 | RPL30 | RPL30 | RPL30 | 2787 | 0.092 | 0.1 | YES |
17 | RPS10 | RPS10 | RPS10 | 2869 | 0.09 | 0.11 | YES |
18 | RPL17 | RPL17 | RPL17 | 2876 | 0.09 | 0.12 | YES |
19 | RPL22 | RPL22 | RPL22 | 2917 | 0.088 | 0.13 | YES |
20 | RPS20 | RPS20 | RPS20 | 2996 | 0.086 | 0.14 | YES |
21 | RPL26 | RPL26 | RPL26 | 3006 | 0.086 | 0.15 | YES |
22 | RPL8 | RPL8 | RPL8 | 3011 | 0.086 | 0.16 | YES |
23 | IPO5 | IPO5 | IPO5 | 3017 | 0.086 | 0.17 | YES |
24 | RPL10A | RPL10A | RPL10A | 3034 | 0.085 | 0.18 | YES |
25 | RPL34 | RPL34 | RPL34 | 3039 | 0.085 | 0.19 | YES |
26 | NUP153 | NUP153 | NUP153 | 3074 | 0.085 | 0.2 | YES |
27 | RPL23 | RPL23 | RPL23 | 3186 | 0.082 | 0.2 | YES |
28 | RPL5 | RPL5 | RPL5 | 3230 | 0.081 | 0.21 | YES |
29 | RPL6 | RPL6 | RPL6 | 3280 | 0.08 | 0.22 | YES |
30 | RPS8 | RPS8 | RPS8 | 3378 | 0.078 | 0.23 | YES |
31 | RPS6 | RPS6 | RPS6 | 3504 | 0.075 | 0.23 | YES |
32 | RPS26 | RPS26 | RPS26 | 3519 | 0.074 | 0.24 | YES |
33 | RPS24 | RPS24 | RPS24 | 3538 | 0.074 | 0.25 | YES |
34 | CLTA | CLTA | CLTA | 3648 | 0.071 | 0.25 | YES |
35 | TPR | TPR | TPR | 3674 | 0.071 | 0.26 | YES |
36 | POLR2K | POLR2K | POLR2K | 3695 | 0.07 | 0.27 | YES |
37 | RPL13A | RPL13A | RPL13A | 3735 | 0.069 | 0.27 | YES |
38 | RPS17 | RPS17 | RPS17 | 3826 | 0.068 | 0.28 | YES |
39 | RPS4X | RPS4X | RPS4X | 3829 | 0.068 | 0.29 | YES |
40 | RPL4 | RPL4 | RPL4 | 3835 | 0.067 | 0.3 | YES |
41 | RPL3 | RPL3 | RPL3 | 3889 | 0.066 | 0.3 | YES |
42 | RPL31 | RPL31 | RPL31 | 3949 | 0.065 | 0.31 | YES |
43 | RPL12 | RPL12 | RPL12 | 3978 | 0.065 | 0.31 | YES |
44 | RPS3 | RPS3 | RPS3 | 3990 | 0.065 | 0.32 | YES |
45 | RPL38 | RPL38 | RPL38 | 4039 | 0.064 | 0.33 | YES |
46 | RPS14 | RPS14 | RPS14 | 4056 | 0.064 | 0.34 | YES |
47 | RPS15A | RPS15A | RPS15A | 4070 | 0.063 | 0.34 | YES |
48 | RPS19 | RPS19 | RPS19 | 4166 | 0.061 | 0.34 | YES |
49 | RPS27A | RPS27A | RPS27A | 4186 | 0.061 | 0.35 | YES |
50 | RPS21 | RPS21 | RPS21 | 4216 | 0.061 | 0.36 | YES |
51 | NUP43 | NUP43 | NUP43 | 4261 | 0.06 | 0.36 | YES |
52 | RPS29 | RPS29 | RPS29 | 4284 | 0.059 | 0.37 | YES |
53 | NUP35 | NUP35 | NUP35 | 4302 | 0.059 | 0.38 | YES |
54 | RPS5 | RPS5 | RPS5 | 4329 | 0.058 | 0.38 | YES |
55 | SEH1L | SEH1L | SEH1L | 4336 | 0.058 | 0.39 | YES |
56 | RPL7A | RPL7A | RPL7A | 4365 | 0.058 | 0.4 | YES |
57 | RPL23A | RPL23A | RPL23A | 4386 | 0.057 | 0.4 | YES |
58 | NUPL1 | NUPL1 | NUPL1 | 4485 | 0.056 | 0.41 | YES |
59 | RPL14 | RPL14 | RPL14 | 4551 | 0.055 | 0.41 | YES |
60 | RPL37 | RPL37 | RPL37 | 4658 | 0.053 | 0.41 | YES |
61 | NUP107 | NUP107 | NUP107 | 4673 | 0.053 | 0.42 | YES |
62 | RPL24 | RPL24 | RPL24 | 4701 | 0.053 | 0.42 | YES |
63 | RPL35A | RPL35A | RPL35A | 4720 | 0.052 | 0.43 | YES |
64 | RPS16 | RPS16 | RPS16 | 4737 | 0.052 | 0.44 | YES |
65 | RPL29 | RPL29 | RPL29 | 4763 | 0.052 | 0.44 | YES |
66 | RPS25 | RPS25 | RPS25 | 4769 | 0.051 | 0.45 | YES |
67 | NUP54 | NUP54 | NUP54 | 4772 | 0.051 | 0.45 | YES |
68 | RPL18 | RPL18 | RPL18 | 4906 | 0.049 | 0.45 | YES |
69 | RPL36 | RPL36 | RPL36 | 4907 | 0.049 | 0.46 | YES |
70 | RPL37A | RPL37A | RPL37A | 4981 | 0.048 | 0.46 | YES |
71 | XPO1 | XPO1 | XPO1 | 4996 | 0.048 | 0.47 | YES |
72 | RPL32 | RPL32 | RPL32 | 5021 | 0.048 | 0.47 | YES |
73 | RPL11 | RPL11 | RPL11 | 5057 | 0.047 | 0.48 | YES |
74 | RPS13 | RPS13 | RPS13 | 5119 | 0.046 | 0.48 | YES |
75 | RPL19 | RPL19 | RPL19 | 5136 | 0.046 | 0.48 | YES |
76 | RPLP1 | RPLP1 | RPLP1 | 5252 | 0.044 | 0.48 | YES |
77 | RPS11 | RPS11 | RPS11 | 5255 | 0.044 | 0.49 | YES |
78 | RPL18A | RPL18A | RPL18A | 5283 | 0.044 | 0.49 | YES |
79 | RPS2 | RPS2 | RPS2 | 5290 | 0.044 | 0.5 | YES |
80 | RPLP0 | RPLP0 | RPLP0 | 5343 | 0.043 | 0.5 | YES |
81 | RPL15 | RPL15 | RPL15 | 5370 | 0.043 | 0.51 | YES |
82 | RPS9 | RPS9 | RPS9 | 5501 | 0.041 | 0.5 | YES |
83 | RPL13 | RPL13 | RPL13 | 5526 | 0.04 | 0.51 | YES |
84 | RPL27 | RPL27 | RPL27 | 5712 | 0.038 | 0.5 | YES |
85 | HSPA1B | HSPA1B | HSPA1B | 5721 | 0.038 | 0.51 | YES |
86 | RPL27A | RPL27A | RPL27A | 5771 | 0.037 | 0.51 | YES |
87 | RPS15 | RPS15 | RPS15 | 5875 | 0.036 | 0.51 | YES |
88 | RPL35 | RPL35 | RPL35 | 5939 | 0.035 | 0.51 | YES |
89 | RPL41 | RPL41 | RPL41 | 5947 | 0.035 | 0.52 | YES |
90 | RPS28 | RPS28 | RPS28 | 6078 | 0.033 | 0.51 | YES |
91 | NUPL2 | NUPL2 | NUPL2 | 6137 | 0.032 | 0.51 | YES |
92 | RPSA | RPSA | RPSA | 6191 | 0.032 | 0.51 | YES |
93 | NUP88 | NUP88 | NUP88 | 6196 | 0.032 | 0.52 | YES |
94 | AAAS | AAAS | AAAS | 6275 | 0.03 | 0.52 | YES |
95 | GRSF1 | GRSF1 | GRSF1 | 6312 | 0.03 | 0.52 | YES |
96 | RAN | RAN | RAN | 6341 | 0.029 | 0.52 | YES |
97 | RPL10 | RPL10 | RPL10 | 6540 | 0.027 | 0.52 | NO |
98 | CANX | CANX | CANX | 6873 | 0.022 | 0.5 | NO |
99 | NUP85 | NUP85 | NUP85 | 6959 | 0.021 | 0.5 | NO |
100 | POLR2B | POLR2B | POLR2B | 6982 | 0.021 | 0.5 | NO |
101 | KPNB1 | KPNB1 | KPNB1 | 7147 | 0.019 | 0.49 | NO |
102 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.48 | NO |
103 | RAE1 | RAE1 | RAE1 | 7320 | 0.016 | 0.49 | NO |
104 | NUP37 | NUP37 | NUP37 | 7365 | 0.016 | 0.49 | NO |
105 | POLR2G | POLR2G | POLR2G | 7554 | 0.014 | 0.48 | NO |
106 | RPLP2 | RPLP2 | RPLP2 | 7610 | 0.013 | 0.48 | NO |
107 | RPL28 | RPL28 | RPL28 | 7615 | 0.013 | 0.48 | NO |
108 | POLR2D | POLR2D | POLR2D | 7717 | 0.012 | 0.47 | NO |
109 | POM121 | POM121 | POM121 | 7942 | 0.0088 | 0.46 | NO |
110 | UBA52 | UBA52 | UBA52 | 8183 | 0.0056 | 0.45 | NO |
111 | RANBP2 | RANBP2 | RANBP2 | 8229 | 0.0051 | 0.45 | NO |
112 | GTF2F1 | GTF2F1 | GTF2F1 | 8371 | 0.0034 | 0.44 | NO |
113 | NUP62 | NUP62 | NUP62 | 8411 | 0.0028 | 0.44 | NO |
114 | NUP50 | NUP50 | NUP50 | 8461 | 0.0023 | 0.44 | NO |
115 | FAU | FAU | FAU | 8710 | -0.0012 | 0.42 | NO |
116 | POLR2E | POLR2E | POLR2E | 8851 | -0.0029 | 0.42 | NO |
117 | POLR2I | POLR2I | POLR2I | 9140 | -0.0063 | 0.4 | NO |
118 | RPS4Y1 | RPS4Y1 | RPS4Y1 | 9221 | -0.0074 | 0.4 | NO |
119 | POLR2F | POLR2F | POLR2F | 9330 | -0.0091 | 0.39 | NO |
120 | NUP155 | NUP155 | NUP155 | 9409 | -0.01 | 0.39 | NO |
121 | NUP205 | NUP205 | NUP205 | 9681 | -0.014 | 0.38 | NO |
122 | CLTC | CLTC | CLTC | 9811 | -0.016 | 0.37 | NO |
123 | POLR2H | POLR2H | POLR2H | 9915 | -0.018 | 0.37 | NO |
124 | DNAJC3 | DNAJC3 | DNAJC3 | 10432 | -0.025 | 0.34 | NO |
125 | NUP188 | NUP188 | NUP188 | 10436 | -0.025 | 0.34 | NO |
126 | POLR2L | POLR2L | POLR2L | 10911 | -0.032 | 0.32 | NO |
127 | RPL26L1 | RPL26L1 | RPL26L1 | 11064 | -0.035 | 0.32 | NO |
128 | KPNA1 | KPNA1 | KPNA1 | 11123 | -0.036 | 0.32 | NO |
129 | POLR2J | POLR2J | POLR2J | 11204 | -0.038 | 0.32 | NO |
130 | NUP93 | NUP93 | NUP93 | 11363 | -0.041 | 0.32 | NO |
131 | CALR | CALR | CALR | 11725 | -0.048 | 0.3 | NO |
132 | POLR2C | POLR2C | POLR2C | 11828 | -0.05 | 0.3 | NO |
133 | POLR2A | POLR2A | POLR2A | 12478 | -0.063 | 0.28 | NO |
134 | NUP210 | NUP210 | NUP210 | 13232 | -0.082 | 0.25 | NO |
135 | FAM153A | FAM153A | FAM153A | 15595 | -0.17 | 0.14 | NO |
Figure S69. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID ATM PATHWAY.

Figure S70. Get High-res Image For the top 5 core enriched genes in the pathway: PID ATM PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S36. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | TAF4B | TAF4B | TAF4B | 548 | 0.22 | 0.025 | YES |
2 | LSM11 | LSM11 | LSM11 | 1072 | 0.17 | 0.038 | YES |
3 | TAF11 | TAF11 | TAF11 | 1198 | 0.16 | 0.07 | YES |
4 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | 0.096 | YES |
5 | GTF2H2B | GTF2H2B | GTF2H2B | 1625 | 0.13 | 0.12 | YES |
6 | GTF2H2 | GTF2H2 | GTF2H2 | 2436 | 0.1 | 0.095 | YES |
7 | CCNT2 | CCNT2 | CCNT2 | 2455 | 0.1 | 0.12 | YES |
8 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | 0.14 | YES |
9 | GTF2F2 | GTF2F2 | GTF2F2 | 2617 | 0.097 | 0.16 | YES |
10 | TAF5 | TAF5 | TAF5 | 2836 | 0.09 | 0.17 | YES |
11 | ZNF473 | ZNF473 | ZNF473 | 2872 | 0.09 | 0.19 | YES |
12 | TCEA1 | TCEA1 | TCEA1 | 3022 | 0.086 | 0.2 | YES |
13 | POLR2K | POLR2K | POLR2K | 3695 | 0.07 | 0.18 | YES |
14 | CCNT1 | CCNT1 | CCNT1 | 3986 | 0.065 | 0.18 | YES |
15 | TBP | TBP | TBP | 3998 | 0.064 | 0.2 | YES |
16 | MNAT1 | MNAT1 | MNAT1 | 4042 | 0.064 | 0.21 | YES |
17 | GTF2H1 | GTF2H1 | GTF2H1 | 4232 | 0.06 | 0.21 | YES |
18 | CSTF3 | CSTF3 | CSTF3 | 4360 | 0.058 | 0.22 | YES |
19 | CPSF1 | CPSF1 | CPSF1 | 4397 | 0.057 | 0.23 | YES |
20 | GTF2B | GTF2B | GTF2B | 4490 | 0.056 | 0.24 | YES |
21 | ERCC2 | ERCC2 | ERCC2 | 4644 | 0.053 | 0.25 | YES |
22 | PCF11 | PCF11 | PCF11 | 4728 | 0.052 | 0.25 | YES |
23 | NFX1 | NFX1 | NFX1 | 4859 | 0.05 | 0.26 | YES |
24 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.27 | YES |
25 | CDK7 | CDK7 | CDK7 | 5105 | 0.046 | 0.27 | YES |
26 | RNMT | RNMT | RNMT | 5197 | 0.045 | 0.28 | YES |
27 | PAPOLA | PAPOLA | PAPOLA | 5248 | 0.044 | 0.28 | YES |
28 | TAF9 | TAF9 | TAF9 | 5333 | 0.043 | 0.29 | YES |
29 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.3 | YES |
30 | PABPN1 | PABPN1 | PABPN1 | 5387 | 0.042 | 0.31 | YES |
31 | NUDT21 | NUDT21 | NUDT21 | 5407 | 0.042 | 0.32 | YES |
32 | SUPT16H | SUPT16H | SUPT16H | 5659 | 0.039 | 0.31 | YES |
33 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.32 | YES |
34 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.33 | YES |
35 | GTF2H4 | GTF2H4 | GTF2H4 | 5810 | 0.037 | 0.33 | YES |
36 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.34 | YES |
37 | TAF1 | TAF1 | TAF1 | 5833 | 0.037 | 0.35 | YES |
38 | GTF2A1 | GTF2A1 | GTF2A1 | 5921 | 0.035 | 0.35 | YES |
39 | TAF4 | TAF4 | TAF4 | 5964 | 0.035 | 0.36 | YES |
40 | SUPT4H1 | SUPT4H1 | SUPT4H1 | 6088 | 0.033 | 0.36 | YES |
41 | TCEB1 | TCEB1 | TCEB1 | 6151 | 0.032 | 0.36 | YES |
42 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.37 | YES |
43 | TH1L | TH1L | TH1L | 6224 | 0.031 | 0.38 | YES |
44 | SLBP | SLBP | SLBP | 6289 | 0.03 | 0.38 | YES |
45 | GTF2H3 | GTF2H3 | GTF2H3 | 6374 | 0.029 | 0.38 | YES |
46 | SSRP1 | SSRP1 | SSRP1 | 6458 | 0.028 | 0.38 | YES |
47 | CCNH | CCNH | CCNH | 6492 | 0.028 | 0.39 | YES |
48 | CSTF1 | CSTF1 | CSTF1 | 6511 | 0.027 | 0.4 | YES |
49 | RNGTT | RNGTT | RNGTT | 6917 | 0.022 | 0.38 | NO |
50 | WHSC2 | WHSC2 | WHSC2 | 6947 | 0.022 | 0.38 | NO |
51 | POLR2B | POLR2B | POLR2B | 6982 | 0.021 | 0.38 | NO |
52 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.37 | NO |
53 | U2AF1 | U2AF1 | U2AF1 | 7354 | 0.016 | 0.37 | NO |
54 | TCEB2 | TCEB2 | TCEB2 | 7362 | 0.016 | 0.38 | NO |
55 | POLR2G | POLR2G | POLR2G | 7554 | 0.014 | 0.37 | NO |
56 | RDBP | RDBP | RDBP | 7667 | 0.012 | 0.37 | NO |
57 | POLR2D | POLR2D | POLR2D | 7717 | 0.012 | 0.37 | NO |
58 | CDC40 | CDC40 | CDC40 | 7784 | 0.011 | 0.36 | NO |
59 | CPSF3 | CPSF3 | CPSF3 | 7830 | 0.01 | 0.36 | NO |
60 | CPSF7 | CPSF7 | CPSF7 | 7905 | 0.0092 | 0.36 | NO |
61 | CLP1 | CLP1 | CLP1 | 8068 | 0.007 | 0.36 | NO |
62 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.35 | NO |
63 | U2AF2 | U2AF2 | U2AF2 | 8289 | 0.0044 | 0.35 | NO |
64 | ERCC3 | ERCC3 | ERCC3 | 8344 | 0.0038 | 0.34 | NO |
65 | GTF2F1 | GTF2F1 | GTF2F1 | 8371 | 0.0034 | 0.34 | NO |
66 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.33 | NO |
67 | CPSF2 | CPSF2 | CPSF2 | 8739 | -0.0015 | 0.32 | NO |
68 | SUPT5H | SUPT5H | SUPT5H | 8819 | -0.0024 | 0.32 | NO |
69 | POLR2E | POLR2E | POLR2E | 8851 | -0.0029 | 0.32 | NO |
70 | SRRM1 | SRRM1 | SRRM1 | 8870 | -0.003 | 0.32 | NO |
71 | POLR2I | POLR2I | POLR2I | 9140 | -0.0063 | 0.3 | NO |
72 | GTF2E1 | GTF2E1 | GTF2E1 | 9228 | -0.0076 | 0.3 | NO |
73 | POLR2F | POLR2F | POLR2F | 9330 | -0.0091 | 0.3 | NO |
74 | THOC4 | THOC4 | THOC4 | 9367 | -0.0097 | 0.3 | NO |
75 | TCEB3 | TCEB3 | TCEB3 | 9396 | -0.01 | 0.3 | NO |
76 | DHX38 | DHX38 | DHX38 | 9443 | -0.011 | 0.3 | NO |
77 | CSTF2 | CSTF2 | CSTF2 | 9466 | -0.011 | 0.3 | NO |
78 | COBRA1 | COBRA1 | COBRA1 | 9803 | -0.016 | 0.29 | NO |
79 | GTF2A2 | GTF2A2 | GTF2A2 | 9857 | -0.017 | 0.29 | NO |
80 | POLR2H | POLR2H | POLR2H | 9915 | -0.018 | 0.29 | NO |
81 | CDK9 | CDK9 | CDK9 | 10723 | -0.03 | 0.25 | NO |
82 | POLR2L | POLR2L | POLR2L | 10911 | -0.032 | 0.25 | NO |
83 | TAF12 | TAF12 | TAF12 | 10912 | -0.032 | 0.26 | NO |
84 | POLR2J | POLR2J | POLR2J | 11204 | -0.038 | 0.25 | NO |
85 | CTDP1 | CTDP1 | CTDP1 | 11289 | -0.039 | 0.26 | NO |
86 | TAF13 | TAF13 | TAF13 | 11645 | -0.046 | 0.25 | NO |
87 | POLR2C | POLR2C | POLR2C | 11828 | -0.05 | 0.25 | NO |
88 | GTF2E2 | GTF2E2 | GTF2E2 | 11847 | -0.05 | 0.26 | NO |
89 | TAF6 | TAF6 | TAF6 | 12000 | -0.053 | 0.26 | NO |
90 | POLR2A | POLR2A | POLR2A | 12478 | -0.063 | 0.25 | NO |
91 | LSM10 | LSM10 | LSM10 | 12758 | -0.069 | 0.26 | NO |
92 | ELL | ELL | ELL | 12837 | -0.071 | 0.27 | NO |
93 | TAF10 | TAF10 | TAF10 | 13417 | -0.087 | 0.26 | NO |
Figure S71. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID RETINOIC ACID PATHWAY.

Figure S72. Get High-res Image For the top 5 core enriched genes in the pathway: PID RETINOIC ACID PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S37. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | -0.0064 | YES |
2 | SMN1 | SMN1 | SMN1 | 2083 | 0.11 | 0.0093 | YES |
3 | TGS1 | TGS1 | TGS1 | 2088 | 0.11 | 0.064 | YES |
4 | NUP133 | NUP133 | NUP133 | 2383 | 0.1 | 0.097 | YES |
5 | SNRPD1 | SNRPD1 | SNRPD1 | 2442 | 0.1 | 0.14 | YES |
6 | NUP153 | NUP153 | NUP153 | 3074 | 0.085 | 0.15 | YES |
7 | CLNS1A | CLNS1A | CLNS1A | 3381 | 0.078 | 0.17 | YES |
8 | GEMIN5 | GEMIN5 | GEMIN5 | 3663 | 0.071 | 0.19 | YES |
9 | TPR | TPR | TPR | 3674 | 0.071 | 0.22 | YES |
10 | SMN2 | SMN2 | SMN2 | 3746 | 0.069 | 0.25 | YES |
11 | NUP43 | NUP43 | NUP43 | 4261 | 0.06 | 0.25 | YES |
12 | NUP35 | NUP35 | NUP35 | 4302 | 0.059 | 0.28 | YES |
13 | SEH1L | SEH1L | SEH1L | 4336 | 0.058 | 0.3 | YES |
14 | NUPL1 | NUPL1 | NUPL1 | 4485 | 0.056 | 0.32 | YES |
15 | NUP107 | NUP107 | NUP107 | 4673 | 0.053 | 0.34 | YES |
16 | NUP54 | NUP54 | NUP54 | 4772 | 0.051 | 0.35 | YES |
17 | GEMIN7 | GEMIN7 | GEMIN7 | 4827 | 0.05 | 0.38 | YES |
18 | PHAX | PHAX | PHAX | 5324 | 0.043 | 0.37 | YES |
19 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.39 | YES |
20 | DDX20 | DDX20 | DDX20 | 5560 | 0.04 | 0.4 | YES |
21 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.41 | YES |
22 | PRMT5 | PRMT5 | PRMT5 | 5746 | 0.038 | 0.42 | YES |
23 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.44 | YES |
24 | GEMIN4 | GEMIN4 | GEMIN4 | 5895 | 0.036 | 0.45 | YES |
25 | NUPL2 | NUPL2 | NUPL2 | 6137 | 0.032 | 0.45 | YES |
26 | NUP88 | NUP88 | NUP88 | 6196 | 0.032 | 0.46 | YES |
27 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.48 | YES |
28 | AAAS | AAAS | AAAS | 6275 | 0.03 | 0.49 | YES |
29 | SNRPD2 | SNRPD2 | SNRPD2 | 6381 | 0.029 | 0.5 | YES |
30 | SNUPN | SNUPN | SNUPN | 6401 | 0.029 | 0.51 | YES |
31 | NUP85 | NUP85 | NUP85 | 6959 | 0.021 | 0.49 | NO |
32 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.48 | NO |
33 | RAE1 | RAE1 | RAE1 | 7320 | 0.016 | 0.48 | NO |
34 | NUP37 | NUP37 | NUP37 | 7365 | 0.016 | 0.49 | NO |
35 | POM121 | POM121 | POM121 | 7942 | 0.0088 | 0.46 | NO |
36 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.45 | NO |
37 | RANBP2 | RANBP2 | RANBP2 | 8229 | 0.0051 | 0.45 | NO |
38 | NUP62 | NUP62 | NUP62 | 8411 | 0.0028 | 0.44 | NO |
39 | NUP50 | NUP50 | NUP50 | 8461 | 0.0023 | 0.44 | NO |
40 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.44 | NO |
41 | WDR77 | WDR77 | WDR77 | 9102 | -0.0058 | 0.41 | NO |
42 | NUP155 | NUP155 | NUP155 | 9409 | -0.01 | 0.4 | NO |
43 | GEMIN6 | GEMIN6 | GEMIN6 | 9444 | -0.011 | 0.4 | NO |
44 | NUP205 | NUP205 | NUP205 | 9681 | -0.014 | 0.39 | NO |
45 | NUP188 | NUP188 | NUP188 | 10436 | -0.025 | 0.36 | NO |
46 | NUP93 | NUP93 | NUP93 | 11363 | -0.041 | 0.33 | NO |
47 | NUP210 | NUP210 | NUP210 | 13232 | -0.082 | 0.27 | NO |
Figure S73. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID BARD1PATHWAY.

Figure S74. Get High-res Image For the top 5 core enriched genes in the pathway: PID BARD1PATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S38. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | POLR1C | POLR1C | POLR1C | 1851 | 0.12 | -0.0085 | YES |
2 | POLR3B | POLR3B | POLR3B | 1924 | 0.12 | 0.079 | YES |
3 | POLR1D | POLR1D | POLR1D | 2447 | 0.1 | 0.13 | YES |
4 | NFIB | NFIB | NFIB | 3346 | 0.078 | 0.14 | YES |
5 | POLR3F | POLR3F | POLR3F | 3435 | 0.076 | 0.19 | YES |
6 | GTF3C4 | GTF3C4 | GTF3C4 | 3441 | 0.076 | 0.25 | YES |
7 | SSB | SSB | SSB | 3629 | 0.072 | 0.29 | YES |
8 | POLR2K | POLR2K | POLR2K | 3695 | 0.07 | 0.34 | YES |
9 | TBP | TBP | TBP | 3998 | 0.064 | 0.38 | YES |
10 | GTF3C3 | GTF3C3 | GTF3C3 | 4713 | 0.052 | 0.38 | YES |
11 | SNAPC1 | SNAPC1 | SNAPC1 | 4850 | 0.05 | 0.41 | YES |
12 | POLR3C | POLR3C | POLR3C | 5184 | 0.045 | 0.42 | YES |
13 | POLR3E | POLR3E | POLR3E | 5320 | 0.043 | 0.45 | YES |
14 | SNAPC4 | SNAPC4 | SNAPC4 | 5900 | 0.036 | 0.44 | YES |
15 | ZNF143 | ZNF143 | ZNF143 | 6404 | 0.029 | 0.44 | YES |
16 | SNAPC3 | SNAPC3 | SNAPC3 | 6536 | 0.027 | 0.45 | YES |
17 | BRF1 | BRF1 | BRF1 | 6738 | 0.024 | 0.46 | YES |
18 | GTF3C5 | GTF3C5 | GTF3C5 | 7060 | 0.02 | 0.46 | NO |
19 | SNAPC5 | SNAPC5 | SNAPC5 | 7452 | 0.015 | 0.45 | NO |
20 | GTF3C2 | GTF3C2 | GTF3C2 | 7790 | 0.011 | 0.44 | NO |
21 | POLR3K | POLR3K | POLR3K | 7797 | 0.01 | 0.44 | NO |
22 | POLR3D | POLR3D | POLR3D | 8015 | 0.0078 | 0.44 | NO |
23 | LZTS1 | LZTS1 | LZTS1 | 8025 | 0.0077 | 0.44 | NO |
24 | BRF2 | BRF2 | BRF2 | 8367 | 0.0035 | 0.43 | NO |
25 | POLR3A | POLR3A | POLR3A | 8709 | -0.0012 | 0.41 | NO |
26 | POU2F1 | POU2F1 | POU2F1 | 8735 | -0.0014 | 0.41 | NO |
27 | POLR3H | POLR3H | POLR3H | 8754 | -0.0018 | 0.41 | NO |
28 | POLR2E | POLR2E | POLR2E | 8851 | -0.0029 | 0.41 | NO |
29 | POLR2F | POLR2F | POLR2F | 9330 | -0.0091 | 0.39 | NO |
30 | POLR2H | POLR2H | POLR2H | 9915 | -0.018 | 0.37 | NO |
31 | POLR3GL | POLR3GL | POLR3GL | 9941 | -0.018 | 0.38 | NO |
32 | POLR2L | POLR2L | POLR2L | 10911 | -0.032 | 0.35 | NO |
33 | SNAPC2 | SNAPC2 | SNAPC2 | 12281 | -0.059 | 0.32 | NO |
Figure S75. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: PID P53REGULATIONPATHWAY.

Figure S76. Get High-res Image For the top 5 core enriched genes in the pathway: PID P53REGULATIONPATHWAY, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S39. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | -0.043 | YES |
2 | HNRNPA1 | HNRNPA1 | HNRNPA1 | 1942 | 0.12 | -0.046 | YES |
3 | NUP133 | NUP133 | NUP133 | 2383 | 0.1 | -0.046 | YES |
4 | SNRPD1 | SNRPD1 | SNRPD1 | 2442 | 0.1 | -0.025 | YES |
5 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | -0.011 | YES |
6 | GTF2F2 | GTF2F2 | GTF2F2 | 2617 | 0.097 | 0.011 | YES |
7 | RBMX | RBMX | RBMX | 3050 | 0.085 | 0.0068 | YES |
8 | NUP153 | NUP153 | NUP153 | 3074 | 0.085 | 0.026 | YES |
9 | RBM5 | RBM5 | RBM5 | 3117 | 0.084 | 0.043 | YES |
10 | TPR | TPR | TPR | 3674 | 0.071 | 0.029 | YES |
11 | HNRNPH1 | HNRNPH1 | HNRNPH1 | 3678 | 0.071 | 0.045 | YES |
12 | POLR2K | POLR2K | POLR2K | 3695 | 0.07 | 0.061 | YES |
13 | HNRNPA3 | HNRNPA3 | HNRNPA3 | 4031 | 0.064 | 0.057 | YES |
14 | NUP43 | NUP43 | NUP43 | 4261 | 0.06 | 0.058 | YES |
15 | NUP35 | NUP35 | NUP35 | 4302 | 0.059 | 0.07 | YES |
16 | METTL3 | METTL3 | METTL3 | 4307 | 0.059 | 0.084 | YES |
17 | SEH1L | SEH1L | SEH1L | 4336 | 0.058 | 0.096 | YES |
18 | CSTF3 | CSTF3 | CSTF3 | 4360 | 0.058 | 0.11 | YES |
19 | CPSF1 | CPSF1 | CPSF1 | 4397 | 0.057 | 0.12 | YES |
20 | HNRNPA0 | HNRNPA0 | HNRNPA0 | 4409 | 0.057 | 0.13 | YES |
21 | SNRNP70 | SNRNP70 | SNRNP70 | 4438 | 0.056 | 0.14 | YES |
22 | NUPL1 | NUPL1 | NUPL1 | 4485 | 0.056 | 0.16 | YES |
23 | DHX9 | DHX9 | DHX9 | 4582 | 0.054 | 0.16 | YES |
24 | NUP107 | NUP107 | NUP107 | 4673 | 0.053 | 0.17 | YES |
25 | LSM2 | LSM2 | LSM2 | 4703 | 0.052 | 0.18 | YES |
26 | PCF11 | PCF11 | PCF11 | 4728 | 0.052 | 0.19 | YES |
27 | NUP54 | NUP54 | NUP54 | 4772 | 0.051 | 0.2 | YES |
28 | NFX1 | NFX1 | NFX1 | 4859 | 0.05 | 0.21 | YES |
29 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.22 | YES |
30 | SMC1A | SMC1A | SMC1A | 5007 | 0.048 | 0.22 | YES |
31 | HNRNPC | HNRNPC | HNRNPC | 5125 | 0.046 | 0.23 | YES |
32 | PAPOLA | PAPOLA | PAPOLA | 5248 | 0.044 | 0.23 | YES |
33 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.24 | YES |
34 | PABPN1 | PABPN1 | PABPN1 | 5387 | 0.042 | 0.24 | YES |
35 | HNRNPM | HNRNPM | HNRNPM | 5392 | 0.042 | 0.25 | YES |
36 | CCAR1 | CCAR1 | CCAR1 | 5394 | 0.042 | 0.26 | YES |
37 | PCBP2 | PCBP2 | PCBP2 | 5401 | 0.042 | 0.27 | YES |
38 | NUDT21 | NUDT21 | NUDT21 | 5407 | 0.042 | 0.28 | YES |
39 | HNRNPU | HNRNPU | HNRNPU | 5423 | 0.042 | 0.29 | YES |
40 | HNRNPD | HNRNPD | HNRNPD | 5462 | 0.041 | 0.3 | YES |
41 | HNRNPA2B1 | HNRNPA2B1 | HNRNPA2B1 | 5503 | 0.041 | 0.31 | YES |
42 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.31 | YES |
43 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.31 | YES |
44 | SF3B1 | SF3B1 | SF3B1 | 5735 | 0.038 | 0.32 | YES |
45 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.33 | YES |
46 | HNRNPR | HNRNPR | HNRNPR | 5834 | 0.037 | 0.33 | YES |
47 | HNRNPK | HNRNPK | HNRNPK | 5861 | 0.036 | 0.34 | YES |
48 | EIF4E | EIF4E | EIF4E | 5918 | 0.035 | 0.34 | YES |
49 | FUS | FUS | FUS | 5948 | 0.035 | 0.35 | YES |
50 | SNRPB2 | SNRPB2 | SNRPB2 | 6105 | 0.033 | 0.35 | YES |
51 | NUPL2 | NUPL2 | NUPL2 | 6137 | 0.032 | 0.36 | YES |
52 | NUP88 | NUP88 | NUP88 | 6196 | 0.032 | 0.36 | YES |
53 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.37 | YES |
54 | SF3B14 | SF3B14 | SF3B14 | 6241 | 0.031 | 0.37 | YES |
55 | AAAS | AAAS | AAAS | 6275 | 0.03 | 0.38 | YES |
56 | SLBP | SLBP | SLBP | 6289 | 0.03 | 0.38 | YES |
57 | SNRPD2 | SNRPD2 | SNRPD2 | 6381 | 0.029 | 0.39 | YES |
58 | NXF1 | NXF1 | NXF1 | 6481 | 0.028 | 0.39 | YES |
59 | SF3A3 | SF3A3 | SF3A3 | 6493 | 0.028 | 0.39 | YES |
60 | CSTF1 | CSTF1 | CSTF1 | 6511 | 0.027 | 0.4 | YES |
61 | SF3B3 | SF3B3 | SF3B3 | 6580 | 0.026 | 0.4 | YES |
62 | YBX1 | YBX1 | YBX1 | 6612 | 0.026 | 0.41 | YES |
63 | SNRPA1 | SNRPA1 | SNRPA1 | 6633 | 0.026 | 0.41 | YES |
64 | NUP85 | NUP85 | NUP85 | 6959 | 0.021 | 0.4 | YES |
65 | HNRNPH2 | HNRNPH2 | HNRNPH2 | 6979 | 0.021 | 0.4 | YES |
66 | POLR2B | POLR2B | POLR2B | 6982 | 0.021 | 0.41 | YES |
67 | HNRNPF | HNRNPF | HNRNPF | 7043 | 0.02 | 0.41 | YES |
68 | HNRNPUL1 | HNRNPUL1 | HNRNPUL1 | 7214 | 0.018 | 0.4 | YES |
69 | NUP214 | NUP214 | NUP214 | 7315 | 0.017 | 0.4 | YES |
70 | RAE1 | RAE1 | RAE1 | 7320 | 0.016 | 0.4 | YES |
71 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.41 | YES |
72 | U2AF1 | U2AF1 | U2AF1 | 7354 | 0.016 | 0.41 | YES |
73 | NUP37 | NUP37 | NUP37 | 7365 | 0.016 | 0.41 | YES |
74 | HNRNPL | HNRNPL | HNRNPL | 7390 | 0.016 | 0.42 | YES |
75 | SNRPA | SNRPA | SNRPA | 7491 | 0.014 | 0.41 | YES |
76 | SNRNP200 | SNRNP200 | SNRNP200 | 7534 | 0.014 | 0.42 | YES |
77 | POLR2G | POLR2G | POLR2G | 7554 | 0.014 | 0.42 | YES |
78 | PRPF4 | PRPF4 | PRPF4 | 7620 | 0.013 | 0.42 | YES |
79 | PTBP1 | PTBP1 | PTBP1 | 7662 | 0.012 | 0.42 | YES |
80 | PRPF6 | PRPF6 | PRPF6 | 7685 | 0.012 | 0.42 | YES |
81 | POLR2D | POLR2D | POLR2D | 7717 | 0.012 | 0.42 | YES |
82 | CDC40 | CDC40 | CDC40 | 7784 | 0.011 | 0.42 | YES |
83 | SF3A2 | SF3A2 | SF3A2 | 7798 | 0.01 | 0.42 | YES |
84 | NHP2L1 | NHP2L1 | NHP2L1 | 7813 | 0.01 | 0.42 | YES |
85 | CPSF3 | CPSF3 | CPSF3 | 7830 | 0.01 | 0.42 | YES |
86 | CPSF7 | CPSF7 | CPSF7 | 7905 | 0.0092 | 0.42 | NO |
87 | POM121 | POM121 | POM121 | 7942 | 0.0088 | 0.42 | NO |
88 | CLP1 | CLP1 | CLP1 | 8068 | 0.007 | 0.42 | NO |
89 | DNAJC8 | DNAJC8 | DNAJC8 | 8124 | 0.0065 | 0.42 | NO |
90 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.41 | NO |
91 | RANBP2 | RANBP2 | RANBP2 | 8229 | 0.0051 | 0.41 | NO |
92 | U2AF2 | U2AF2 | U2AF2 | 8289 | 0.0044 | 0.41 | NO |
93 | PRPF8 | PRPF8 | PRPF8 | 8292 | 0.0044 | 0.41 | NO |
94 | DDX23 | DDX23 | DDX23 | 8310 | 0.0042 | 0.41 | NO |
95 | GTF2F1 | GTF2F1 | GTF2F1 | 8371 | 0.0034 | 0.41 | NO |
96 | EFTUD2 | EFTUD2 | EFTUD2 | 8402 | 0.003 | 0.41 | NO |
97 | NUP62 | NUP62 | NUP62 | 8411 | 0.0028 | 0.41 | NO |
98 | NUP50 | NUP50 | NUP50 | 8461 | 0.0023 | 0.4 | NO |
99 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.4 | NO |
100 | CPSF2 | CPSF2 | CPSF2 | 8739 | -0.0015 | 0.39 | NO |
101 | TXNL4A | TXNL4A | TXNL4A | 8755 | -0.0018 | 0.39 | NO |
102 | POLR2E | POLR2E | POLR2E | 8851 | -0.0029 | 0.38 | NO |
103 | SRRM1 | SRRM1 | SRRM1 | 8870 | -0.003 | 0.38 | NO |
104 | SF3B4 | SF3B4 | SF3B4 | 8872 | -0.003 | 0.38 | NO |
105 | SNRNP40 | SNRNP40 | SNRNP40 | 8967 | -0.0041 | 0.38 | NO |
106 | POLR2I | POLR2I | POLR2I | 9140 | -0.0063 | 0.37 | NO |
107 | POLR2F | POLR2F | POLR2F | 9330 | -0.0091 | 0.36 | NO |
108 | THOC4 | THOC4 | THOC4 | 9367 | -0.0097 | 0.36 | NO |
109 | NUP155 | NUP155 | NUP155 | 9409 | -0.01 | 0.36 | NO |
110 | DHX38 | DHX38 | DHX38 | 9443 | -0.011 | 0.37 | NO |
111 | CSTF2 | CSTF2 | CSTF2 | 9466 | -0.011 | 0.37 | NO |
112 | SF3A1 | SF3A1 | SF3A1 | 9630 | -0.013 | 0.36 | NO |
113 | NUP205 | NUP205 | NUP205 | 9681 | -0.014 | 0.36 | NO |
114 | SF3B2 | SF3B2 | SF3B2 | 9729 | -0.015 | 0.36 | NO |
115 | SF3B5 | SF3B5 | SF3B5 | 9742 | -0.015 | 0.36 | NO |
116 | PHF5A | PHF5A | PHF5A | 9914 | -0.018 | 0.36 | NO |
117 | POLR2H | POLR2H | POLR2H | 9915 | -0.018 | 0.36 | NO |
118 | PCBP1 | PCBP1 | PCBP1 | 10196 | -0.022 | 0.35 | NO |
119 | CD2BP2 | CD2BP2 | CD2BP2 | 10411 | -0.025 | 0.35 | NO |
120 | NUP188 | NUP188 | NUP188 | 10436 | -0.025 | 0.35 | NO |
121 | POLR2L | POLR2L | POLR2L | 10911 | -0.032 | 0.33 | NO |
122 | POLR2J | POLR2J | POLR2J | 11204 | -0.038 | 0.33 | NO |
123 | NUP93 | NUP93 | NUP93 | 11363 | -0.041 | 0.33 | NO |
124 | POLR2C | POLR2C | POLR2C | 11828 | -0.05 | 0.31 | NO |
125 | POLR2A | POLR2A | POLR2A | 12478 | -0.063 | 0.29 | NO |
126 | NUP210 | NUP210 | NUP210 | 13232 | -0.082 | 0.27 | NO |
Figure S77. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: REACTOME TRANSLATION.

Figure S78. Get High-res Image For the top 5 core enriched genes in the pathway: REACTOME TRANSLATION, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

Table S40. Get Full Table This table shows a Running Enrichment Score (RES) of each gene in this pathway, that is, the enrichment score at this point in the ranked list of genes. All genes are ranked by Signal-to-Noise (S2N), a measure of similarity as default and are used to obtain ES matrix of all genes. In this way, GSEA tool uses expression pattern of not only overlapped genes but also not-overlapped genes to produce ES matrix.
Rank | GENE | SYMBOL | DESC | LIST.LOC | S2N | RES | CORE_ENRICHMENT |
---|---|---|---|---|---|---|---|
1 | LSM11 | LSM11 | LSM11 | 1072 | 0.17 | 0.068 | YES |
2 | SNRPE | SNRPE | SNRPE | 1385 | 0.15 | 0.16 | YES |
3 | UPF3B | UPF3B | UPF3B | 2602 | 0.097 | 0.17 | YES |
4 | ZNF473 | ZNF473 | ZNF473 | 2872 | 0.09 | 0.22 | YES |
5 | CSTF3 | CSTF3 | CSTF3 | 4360 | 0.058 | 0.18 | YES |
6 | CPSF1 | CPSF1 | CPSF1 | 4397 | 0.057 | 0.22 | YES |
7 | PCF11 | PCF11 | PCF11 | 4728 | 0.052 | 0.24 | YES |
8 | NFX1 | NFX1 | NFX1 | 4859 | 0.05 | 0.27 | YES |
9 | RBM8A | RBM8A | RBM8A | 4912 | 0.049 | 0.31 | YES |
10 | PAPOLA | PAPOLA | PAPOLA | 5248 | 0.044 | 0.32 | YES |
11 | SNRPF | SNRPF | SNRPF | 5362 | 0.043 | 0.35 | YES |
12 | PABPN1 | PABPN1 | PABPN1 | 5387 | 0.042 | 0.38 | YES |
13 | NUDT21 | NUDT21 | NUDT21 | 5407 | 0.042 | 0.41 | YES |
14 | SNRPD3 | SNRPD3 | SNRPD3 | 5680 | 0.038 | 0.42 | YES |
15 | MAGOH | MAGOH | MAGOH | 5697 | 0.038 | 0.45 | YES |
16 | NCBP1 | NCBP1 | NCBP1 | 5811 | 0.037 | 0.47 | YES |
17 | SNRPB | SNRPB | SNRPB | 6218 | 0.031 | 0.47 | YES |
18 | SLBP | SLBP | SLBP | 6289 | 0.03 | 0.49 | YES |
19 | CSTF1 | CSTF1 | CSTF1 | 6511 | 0.027 | 0.5 | YES |
20 | RNPS1 | RNPS1 | RNPS1 | 7328 | 0.016 | 0.47 | NO |
21 | U2AF1 | U2AF1 | U2AF1 | 7354 | 0.016 | 0.48 | NO |
22 | CDC40 | CDC40 | CDC40 | 7784 | 0.011 | 0.46 | NO |
23 | CPSF3 | CPSF3 | CPSF3 | 7830 | 0.01 | 0.47 | NO |
24 | CPSF7 | CPSF7 | CPSF7 | 7905 | 0.0092 | 0.47 | NO |
25 | CLP1 | CLP1 | CLP1 | 8068 | 0.007 | 0.47 | NO |
26 | NCBP2 | NCBP2 | NCBP2 | 8179 | 0.0056 | 0.46 | NO |
27 | U2AF2 | U2AF2 | U2AF2 | 8289 | 0.0044 | 0.46 | NO |
28 | SNRPG | SNRPG | SNRPG | 8560 | 0.001 | 0.45 | NO |
29 | CPSF2 | CPSF2 | CPSF2 | 8739 | -0.0015 | 0.44 | NO |
30 | SRRM1 | SRRM1 | SRRM1 | 8870 | -0.003 | 0.43 | NO |
31 | THOC4 | THOC4 | THOC4 | 9367 | -0.0097 | 0.41 | NO |
32 | DHX38 | DHX38 | DHX38 | 9443 | -0.011 | 0.42 | NO |
33 | CSTF2 | CSTF2 | CSTF2 | 9466 | -0.011 | 0.42 | NO |
34 | LSM10 | LSM10 | LSM10 | 12758 | -0.069 | 0.29 | NO |
Figure S79. Get High-res Image This plot shows mRNAseq_cNMF expression data heatmap (on the left) a RunningEnrichmentScore(RES) plot (on the top right) and a Signal2Noise(S2N) plot (on the bottom right) of genes in the pathway: REACTOME FORMATION OF THE TERNARY COMPLEX AND SUBSEQUENTLY THE 43S COMPLEX.

Figure S80. Get High-res Image For the top 5 core enriched genes in the pathway: REACTOME FORMATION OF THE TERNARY COMPLEX AND SUBSEQUENTLY THE 43S COMPLEX, this volcano plot shows how much they are up/down-regulated and significant. The significance was calculated by empirical bayesian fit

For the top enriched genes, if you want to check whether they are
-
up-regulated, please check the list of up-regulated genes
-
down-regulated, please check the list of down-regulated genes
For the top enriched genes, if you want to check whether they are
-
highly expressed genes, please check the list of high (top 30%) expressed genes
-
low expressed genes, please check the list of low (bottom 30%) expressed genes
An expression pattern of top(30%)/middle(30%)/low(30%) in this subtype against other subtypes is available in a heatmap
For the top enriched genes, if you want to check whether they are
-
significantly differently expressed genes by eBayes lm fit, please check the list of significant genes
-
Gene set database = c2.cp.v4.0.symbols.gmt
-
Expression data file = SKCM-TM.uncv2.mRNAseq_RSEM_normalized_log2.txt
-
Phenotype data file = SKCM-TM.mergedcluster.txt
For the Gene Set Enrichment Analysis (GSEA), Broad GSEA-P-R.1.0 version is used with class2: canonical pathways geneses from MSigDB. Further details about statistics are available inThe Broad GSEA website.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.