GDAC_RNAseq_ConsensusClusteringPipeline Execution Log 11:21 AM Tue Aug 28, '12

Running as user: cgaadm_deadline

GDAC_TopgenesforCluster1 expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/BRCA/1676449/0.mRNAseq_preprocessor.Finished/BRCA.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_TopgenesforCluster1 selectedgenes 1500
GDAC_ConsensusClustering2 clustering algorithm HIERARCHICAL
GDAC_ConsensusClustering2 cluster by
GDAC_ConsensusClustering2 distance measure PEARSON
GDAC_ConsensusClustering2 normalize type -n2
GDAC_selectBestcluster3 measure Pearson
GDAC_selectBestcluster3 output BRCA
GDAC_selectBestcluster3 inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/BRCA/1676449/0.mRNAseq_preprocessor.Finished/BRCA.uncv2.mRNAseq_RSEM_normalized_log2.txt
GDAC_CnmfReports4 report hclumRNAseq
Execution Times:
Submitted: 11:21:29 28-08-12
Completed:
Elapsed: 01 hrs 05 mins 39 secs

step 1. GDAC_TopgenesforCluster [id: 157516] This module can be used to selecte top genes with most variation, convert file to gct format, as well as imput missing value

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00205:29
expfile file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/BRCA/1676449/0.mRNAseq_preprocessor.Finished/BRCA.uncv2.mRNAseq_RSEM_normalized_log2.txt
selectedgenes 1500
outputprefix outputprefix
Output Files:
 outputprefix.expclu.gct
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 11:21:29 28-08-12
Completed: 11:30:28 28-08-12
Elapsed: 00 hrs 08 mins 58 secs

step 2. GDAC_ConsensusClustering [id: 157517] Resampling-based clustering method

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00158:6
input filename outputprefix.expclu.gct
kmax 8
resampling iterations 20
seed value 12345
clustering algorithm HIERARCHICAL
cluster by
distance measure PEARSON
resample subsample
merge type average
descent iterations 2000
output stub <input.filename_basename>
normalize type -n2
normalization iterations 0
create heat map -p
heat map size 2
Output Files:
 outputprefix.expclu.sub644.stdev
 outputprefix.expclu.sub644.clsdist
 outputprefix.expclu.sub644.2.gct
 outputprefix.expclu.sub644.3.gct
 outputprefix.expclu.sub644.4.gct
 outputprefix.expclu.sub644.5.gct
 outputprefix.expclu.sub644.6.gct
 outputprefix.expclu.sub644.7.gct
 outputprefix.expclu.sub644.8.gct
 outputprefix.expclu.sub644.2.clu
 outputprefix.expclu.sub644.3.clu
 outputprefix.expclu.sub644.4.clu
 outputprefix.expclu.sub644.5.clu
 outputprefix.expclu.sub644.6.clu
 outputprefix.expclu.sub644.7.clu
 outputprefix.expclu.sub644.8.clu
 outputprefix.expclu.sub644.statistics.pdf
 outputprefix.expclu.sub644.srt.2.gct
 outputprefix.expclu.sub644.srt.2.gif
 outputprefix.expclu.sub644.srt.3.gct
 outputprefix.expclu.sub644.srt.3.gif
 outputprefix.expclu.sub644.srt.4.gct
 outputprefix.expclu.sub644.srt.4.gif
 outputprefix.expclu.sub644.srt.5.gct
 outputprefix.expclu.sub644.srt.5.gif
 outputprefix.expclu.sub644.srt.6.gif
 outputprefix.expclu.sub644.srt.6.gct
 outputprefix.expclu.sub644.srt.7.gct
 outputprefix.expclu.sub644.srt.7.gif
 outputprefix.expclu.sub644.srt.8.gct
 outputprefix.expclu.sub644.srt.8.gif
 stdout.txt
Execution Times:
Submitted: 11:21:29 28-08-12
Completed: 12:20:10 28-08-12
Elapsed: 00 hrs 58 mins 40 secs

step 3. GDAC_selectBestcluster [id: 157518]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00207:32
measure Pearson
inputexp outputprefix.expclu.gct
output BRCA
file clu 2 outputprefix.expclu.sub644.2.clu
file clu 3 outputprefix.expclu.sub644.3.clu
file clu 4 outputprefix.expclu.sub644.4.clu
file clu 5 outputprefix.expclu.sub644.5.clu
file clu 6 outputprefix.expclu.sub644.6.clu
file clu 7 outputprefix.expclu.sub644.7.clu
file clu 8 outputprefix.expclu.sub644.8.clu
inputallexp file:/xchip/cga/gdac-prod/tcga-gdac/jobResults/mRNAseq_preprocessor/BRCA/1676449/0.mRNAseq_preprocessor.Finished/BRCA.uncv2.mRNAseq_RSEM_normalized_log2.txt
Output Files:
 BRCA.silfig.png
 BRCA.cormatrix.png
 BRCA.bestclus.txt
 BRCA.allclusters.txt
 BRCA.subclassmarkers.txt
 BRCA.seclectedSubclassmarkers.txt
 BRCA.geneheatmap.png
 BRCA.geneheatmaptopgenes.png
 .lsf.out
 stdout.txt
Execution Times:
Submitted: 11:21:29 28-08-12
Completed: 12:26:38 28-08-12
Elapsed: 01 hrs 05 mins 08 secs

step 4. GDAC_CnmfReports [id: 157519]

    urn:lsid:broadinstitute.org:cancer.genome.analysis:00414:21