This pipeline uses various statistical tests to identify mRNAs whose expression levels correlated to selected clinical features.
Testing the association between 17814 genes and 4 clinical features across 25 samples, statistically thresholded by P value <= 0.05 and Q value <= 0.25
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1 gene correlated to 'GENDER'.
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MRPL3
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1 gene correlated to 'PATHOLOGY.T'.
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PAPPA2
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No genes correlated to 'AGE', and 'PATHOLOGY.N'.
Complete statistical result table is provided in Supplement Table 1
Table 1. Get Full Table This table shows the clinical features, statistical methods used, and the number of genes that are significantly associated with each clinical feature at P value <= 0.05 and Q value <= 0.25.
Clinical feature | Statistical test | Significant genes | Associated with | Associated with | ||
AGE | Spearman correlation test | N=0 | ||||
GENDER | t test | N=1 | male | N=1 | female | N=0 |
PATHOLOGY T | Spearman correlation test | N=1 | higher pT | N=0 | lower pT | N=1 |
PATHOLOGY N | Spearman correlation test | N=0 |
Table 2. Basic characteristics of clinical feature: 'AGE'
AGE | Mean (SD) | 64.16 (10) |
Significant markers | N = 0 |
Table 3. Basic characteristics of clinical feature: 'GENDER'
GENDER | Labels | N |
FEMALE | 12 | |
MALE | 13 | |
Significant markers | N = 1 | |
Higher in MALE | 1 | |
Higher in FEMALE | 0 |
Table 4. Get Full Table List of one gene differentially expressed by 'GENDER'
T | ttestP | Q | AUC | |
MRPL3 | 5.51 | 1.318e-05 | 0.235 | 0.9487 |
Figure 1. Get High-res Image As an example, this figure shows the association of MRPL3 to 'GENDER'. P value = 1.32e-05 with T-test analysis.
![](V2ex.png)
Table 5. Basic characteristics of clinical feature: 'PATHOLOGY.T'
PATHOLOGY.T | Mean (SD) | 1.71 (0.55) |
N | ||
T1 | 8 | |
T2 | 15 | |
T3 | 1 | |
Significant markers | N = 1 | |
pos. correlated | 0 | |
neg. correlated | 1 |
Table 6. Get Full Table List of one gene significantly correlated to 'PATHOLOGY.T' by Spearman correlation test
SpearmanCorr | corrP | Q | |
PAPPA2 | -0.766 | 1.28e-05 | 0.228 |
Figure 2. Get High-res Image As an example, this figure shows the association of PAPPA2 to 'PATHOLOGY.T'. P value = 1.28e-05 with Spearman correlation analysis.
![](V3ex.png)
Input Data
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Expresson data file = LUAD.medianexp.txt
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Clinical data file = LUAD.clin.merged.picked.txt
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Number of samples = 25
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Number of genes = 17814
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Number of clinical features = 4
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p value cutoff = 0.05
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q value cutoff = 0.25