This pipeline computes the correlation between significant arm-level copy number variations (cnvs) and selected clinical features.
Testing the association between copy number variation 71 arm-level results and 9 clinical features across 490 patients, 3 significant findings detected with Q value < 0.25.
-
5p gain cnv correlated to 'AGE'.
-
5q gain cnv correlated to 'AGE'.
-
11p loss cnv correlated to 'Time to Death'.
Clinical Features |
Time to Death |
AGE | GENDER |
KARNOFSKY PERFORMANCE SCORE |
PATHOLOGY T |
PATHOLOGY N |
PATHOLOGICSPREAD(M) |
TUMOR STAGE |
NEOADJUVANT THERAPY |
||
nCNV (%) | nWild-Type | logrank test | t-test | Fisher's exact test | t-test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | |
5p gain | 141 (29%) | 349 |
0.199 (1.00) |
0.000105 (0.0638) |
0.143 (1.00) |
0.563 (1.00) |
0.169 (1.00) |
1 (1.00) |
0.783 (1.00) |
0.215 (1.00) |
0.629 (1.00) |
5q gain | 155 (32%) | 335 |
0.341 (1.00) |
0.000273 (0.165) |
0.155 (1.00) |
0.482 (1.00) |
0.206 (1.00) |
0.616 (1.00) |
0.594 (1.00) |
0.394 (1.00) |
0.654 (1.00) |
11p loss | 5 (1%) | 485 |
4.3e-05 (0.0261) |
0.936 (1.00) |
0.662 (1.00) |
0.728 (1.00) |
0.142 (1.00) |
0.571 (1.00) |
0.909 (1.00) |
1 (1.00) |
|
1p gain | 6 (1%) | 484 |
0.162 (1.00) |
0.641 (1.00) |
0.425 (1.00) |
0.289 (1.00) |
0.205 (1.00) |
1 (1.00) |
0.222 (1.00) |
1 (1.00) |
|
1q gain | 34 (7%) | 456 |
0.973 (1.00) |
0.0904 (1.00) |
0.578 (1.00) |
0.115 (1.00) |
0.094 (1.00) |
0.361 (1.00) |
1 (1.00) |
0.105 (1.00) |
0.303 (1.00) |
2p gain | 47 (10%) | 443 |
0.719 (1.00) |
0.0895 (1.00) |
0.149 (1.00) |
0.563 (1.00) |
0.704 (1.00) |
1 (1.00) |
0.205 (1.00) |
0.278 (1.00) |
1 (1.00) |
2q gain | 48 (10%) | 442 |
0.522 (1.00) |
0.141 (1.00) |
0.0254 (1.00) |
0.159 (1.00) |
0.843 (1.00) |
1 (1.00) |
0.206 (1.00) |
0.373 (1.00) |
1 (1.00) |
3p gain | 7 (1%) | 483 |
0.945 (1.00) |
0.421 (1.00) |
0.245 (1.00) |
0.456 (1.00) |
0.264 (1.00) |
0.297 (1.00) |
0.122 (1.00) |
0.0697 (1.00) |
|
3q gain | 26 (5%) | 464 |
0.765 (1.00) |
0.846 (1.00) |
1 (1.00) |
0.0261 (1.00) |
0.178 (1.00) |
0.332 (1.00) |
0.157 (1.00) |
0.218 (1.00) |
0.0246 (1.00) |
4p gain | 8 (2%) | 482 |
0.24 (1.00) |
0.86 (1.00) |
0.719 (1.00) |
0.357 (1.00) |
0.37 (1.00) |
0.616 (1.00) |
0.0904 (1.00) |
1 (1.00) |
|
4q gain | 8 (2%) | 482 |
0.661 (1.00) |
0.38 (1.00) |
1 (1.00) |
0.562 (1.00) |
0.37 (1.00) |
0.616 (1.00) |
0.215 (1.00) |
1 (1.00) |
|
6p gain | 6 (1%) | 484 |
0.229 (1.00) |
0.231 (1.00) |
1 (1.00) |
0.664 (1.00) |
1 (1.00) |
1 (1.00) |
0.757 (1.00) |
1 (1.00) |
|
6q gain | 5 (1%) | 485 |
0.187 (1.00) |
0.436 (1.00) |
0.662 (1.00) |
0.407 (1.00) |
1 (1.00) |
0.571 (1.00) |
0.371 (1.00) |
1 (1.00) |
|
7p gain | 126 (26%) | 364 |
0.773 (1.00) |
0.275 (1.00) |
0.104 (1.00) |
0.893 (1.00) |
0.253 (1.00) |
0.6 (1.00) |
0.0448 (1.00) |
0.169 (1.00) |
0.607 (1.00) |
7q gain | 128 (26%) | 362 |
0.444 (1.00) |
0.338 (1.00) |
0.067 (1.00) |
0.699 (1.00) |
0.106 (1.00) |
1 (1.00) |
0.00653 (1.00) |
0.0429 (1.00) |
0.115 (1.00) |
8p gain | 14 (3%) | 476 |
0.569 (1.00) |
0.745 (1.00) |
0.0418 (1.00) |
0.757 (1.00) |
0.00707 (1.00) |
1 (1.00) |
0.464 (1.00) |
0.00296 (1.00) |
1 (1.00) |
8q gain | 33 (7%) | 457 |
0.641 (1.00) |
0.293 (1.00) |
0.256 (1.00) |
0.471 (1.00) |
0.00283 (1.00) |
0.648 (1.00) |
1 (1.00) |
0.00149 (0.894) |
1 (1.00) |
9p gain | 8 (2%) | 482 |
0.122 (1.00) |
0.0356 (1.00) |
0.024 (1.00) |
0.615 (1.00) |
1 (1.00) |
0.616 (1.00) |
0.381 (1.00) |
1 (1.00) |
|
9q gain | 8 (2%) | 482 |
0.772 (1.00) |
0.959 (1.00) |
0.459 (1.00) |
0.0078 (1.00) |
1 (1.00) |
0.359 (1.00) |
0.113 (1.00) |
1 (1.00) |
|
10p gain | 5 (1%) | 485 |
0.143 (1.00) |
0.137 (1.00) |
0.348 (1.00) |
0.348 (1.00) |
1 (1.00) |
0.174 (1.00) |
0.188 (1.00) |
0.0502 (1.00) |
|
10q gain | 4 (1%) | 486 |
0.517 (1.00) |
0.171 (1.00) |
0.125 (1.00) |
0.649 (1.00) |
1 (1.00) |
0.492 (1.00) |
0.38 (1.00) |
0.0403 (1.00) |
|
11p gain | 17 (3%) | 473 |
0.968 (1.00) |
0.818 (1.00) |
0.609 (1.00) |
0.115 (1.00) |
0.0219 (1.00) |
1 (1.00) |
0.161 (1.00) |
0.0209 (1.00) |
0.162 (1.00) |
11q gain | 15 (3%) | 475 |
0.314 (1.00) |
0.793 (1.00) |
0.41 (1.00) |
0.074 (1.00) |
1 (1.00) |
0.714 (1.00) |
0.0465 (1.00) |
0.145 (1.00) |
|
12p gain | 77 (16%) | 413 |
0.076 (1.00) |
0.0827 (1.00) |
0.363 (1.00) |
0.404 (1.00) |
0.0353 (1.00) |
1 (1.00) |
0.0881 (1.00) |
0.0259 (1.00) |
0.177 (1.00) |
12q gain | 77 (16%) | 413 |
0.0995 (1.00) |
0.125 (1.00) |
0.363 (1.00) |
0.404 (1.00) |
0.0353 (1.00) |
0.747 (1.00) |
0.0881 (1.00) |
0.0259 (1.00) |
0.177 (1.00) |
13q gain | 14 (3%) | 476 |
0.289 (1.00) |
0.836 (1.00) |
0.779 (1.00) |
0.323 (1.00) |
1 (1.00) |
1 (1.00) |
0.25 (1.00) |
0.00723 (1.00) |
|
14q gain | 5 (1%) | 485 |
0.444 (1.00) |
0.517 (1.00) |
1 (1.00) |
0.0221 (1.00) |
1 (1.00) |
0.571 (1.00) |
0.133 (1.00) |
1 (1.00) |
|
15q gain | 14 (3%) | 476 |
0.859 (1.00) |
0.957 (1.00) |
0.779 (1.00) |
0.242 (1.00) |
0.462 (1.00) |
0.464 (1.00) |
0.417 (1.00) |
1 (1.00) |
|
16p gain | 65 (13%) | 425 |
0.595 (1.00) |
0.0727 (1.00) |
0.264 (1.00) |
0.4 (1.00) |
0.825 (1.00) |
0.0836 (1.00) |
0.715 (1.00) |
0.742 (1.00) |
0.133 (1.00) |
16q gain | 58 (12%) | 432 |
0.474 (1.00) |
0.0946 (1.00) |
0.107 (1.00) |
0.191 (1.00) |
0.679 (1.00) |
0.143 (1.00) |
1 (1.00) |
0.74 (1.00) |
0.469 (1.00) |
17p gain | 16 (3%) | 474 |
0.466 (1.00) |
0.549 (1.00) |
0.796 (1.00) |
0.281 (1.00) |
0.503 (1.00) |
0.15 (1.00) |
0.147 (1.00) |
0.154 (1.00) |
|
17q gain | 22 (4%) | 468 |
0.149 (1.00) |
0.558 (1.00) |
0.648 (1.00) |
0.161 (1.00) |
0.329 (1.00) |
1 (1.00) |
0.553 (1.00) |
0.433 (1.00) |
0.206 (1.00) |
18p gain | 18 (4%) | 472 |
0.087 (1.00) |
0.8 (1.00) |
0.023 (1.00) |
0.963 (1.00) |
1 (1.00) |
0.332 (1.00) |
0.515 (1.00) |
1 (1.00) |
|
18q gain | 18 (4%) | 472 |
0.087 (1.00) |
0.8 (1.00) |
0.023 (1.00) |
0.963 (1.00) |
1 (1.00) |
0.332 (1.00) |
0.515 (1.00) |
1 (1.00) |
|
19p gain | 25 (5%) | 465 |
0.926 (1.00) |
0.724 (1.00) |
1 (1.00) |
0.161 (1.00) |
0.541 (1.00) |
0.303 (1.00) |
0.253 (1.00) |
0.228 (1.00) |
0.231 (1.00) |
19q gain | 28 (6%) | 462 |
0.874 (1.00) |
0.731 (1.00) |
0.684 (1.00) |
0.161 (1.00) |
0.484 (1.00) |
0.361 (1.00) |
0.175 (1.00) |
0.238 (1.00) |
0.0283 (1.00) |
20p gain | 65 (13%) | 425 |
0.196 (1.00) |
0.199 (1.00) |
0.0171 (1.00) |
0.315 (1.00) |
0.00354 (1.00) |
0.483 (1.00) |
0.0413 (1.00) |
0.0147 (1.00) |
0.511 (1.00) |
20q gain | 67 (14%) | 423 |
0.328 (1.00) |
0.161 (1.00) |
0.0266 (1.00) |
0.315 (1.00) |
0.00614 (1.00) |
0.483 (1.00) |
0.103 (1.00) |
0.0238 (1.00) |
0.522 (1.00) |
21q gain | 32 (7%) | 458 |
0.209 (1.00) |
0.226 (1.00) |
0.848 (1.00) |
0.161 (1.00) |
0.488 (1.00) |
1 (1.00) |
1 (1.00) |
0.903 (1.00) |
1 (1.00) |
22q gain | 25 (5%) | 465 |
0.953 (1.00) |
0.851 (1.00) |
0.524 (1.00) |
0.2 (1.00) |
1 (1.00) |
0.253 (1.00) |
0.45 (1.00) |
1 (1.00) |
|
Xq gain | 11 (2%) | 479 |
0.447 (1.00) |
0.711 (1.00) |
0.0558 (1.00) |
0.871 (1.00) |
1 (1.00) |
0.0747 (1.00) |
0.0474 (1.00) |
0.108 (1.00) |
|
1p loss | 31 (6%) | 459 |
0.985 (1.00) |
0.167 (1.00) |
0.562 (1.00) |
0.115 (1.00) |
0.0192 (1.00) |
0.607 (1.00) |
0.45 (1.00) |
0.0922 (1.00) |
1 (1.00) |
1q loss | 21 (4%) | 469 |
0.769 (1.00) |
0.62 (1.00) |
0.644 (1.00) |
0.0851 (1.00) |
1 (1.00) |
0.756 (1.00) |
0.256 (1.00) |
1 (1.00) |
|
2p loss | 10 (2%) | 480 |
0.466 (1.00) |
0.946 (1.00) |
0.506 (1.00) |
0.0928 (1.00) |
1 (1.00) |
1 (1.00) |
0.483 (1.00) |
1 (1.00) |
|
2q loss | 11 (2%) | 479 |
0.848 (1.00) |
0.998 (1.00) |
0.343 (1.00) |
0.194 (1.00) |
1 (1.00) |
1 (1.00) |
0.747 (1.00) |
1 (1.00) |
|
3p loss | 303 (62%) | 187 |
0.512 (1.00) |
0.366 (1.00) |
0.064 (1.00) |
0.906 (1.00) |
0.0106 (1.00) |
0.463 (1.00) |
0.0067 (1.00) |
0.0146 (1.00) |
0.375 (1.00) |
3q loss | 73 (15%) | 417 |
0.938 (1.00) |
0.0261 (1.00) |
0.000792 (0.478) |
0.573 (1.00) |
0.352 (1.00) |
1 (1.00) |
0.486 (1.00) |
0.524 (1.00) |
0.555 (1.00) |
4p loss | 34 (7%) | 456 |
0.0917 (1.00) |
0.713 (1.00) |
0.711 (1.00) |
0.0176 (1.00) |
0.0166 (1.00) |
1 (1.00) |
0.632 (1.00) |
0.083 (1.00) |
0.303 (1.00) |
4q loss | 28 (6%) | 462 |
0.0188 (1.00) |
0.967 (1.00) |
0.84 (1.00) |
0.0261 (1.00) |
0.00362 (1.00) |
1 (1.00) |
0.175 (1.00) |
0.0271 (1.00) |
0.256 (1.00) |
6p loss | 57 (12%) | 433 |
0.329 (1.00) |
0.192 (1.00) |
0.00299 (1.00) |
0.499 (1.00) |
0.88 (1.00) |
0.171 (1.00) |
0.435 (1.00) |
0.817 (1.00) |
0.463 (1.00) |
6q loss | 82 (17%) | 408 |
0.532 (1.00) |
0.762 (1.00) |
0.1 (1.00) |
0.851 (1.00) |
0.969 (1.00) |
0.0601 (1.00) |
0.869 (1.00) |
0.761 (1.00) |
1 (1.00) |
8p loss | 94 (19%) | 396 |
0.53 (1.00) |
0.108 (1.00) |
0.279 (1.00) |
0.0817 (1.00) |
0.624 (1.00) |
0.322 (1.00) |
0.431 (1.00) |
0.544 (1.00) |
1 (1.00) |
8q loss | 37 (8%) | 453 |
0.275 (1.00) |
0.944 (1.00) |
0.0469 (1.00) |
0.00419 (1.00) |
0.988 (1.00) |
0.608 (1.00) |
0.243 (1.00) |
0.854 (1.00) |
1 (1.00) |
9p loss | 87 (18%) | 403 |
0.012 (1.00) |
0.0721 (1.00) |
0.0128 (1.00) |
0.0261 (1.00) |
0.2 (1.00) |
0.745 (1.00) |
0.0213 (1.00) |
0.0986 (1.00) |
0.217 (1.00) |
9q loss | 92 (19%) | 398 |
0.00591 (1.00) |
0.04 (1.00) |
0.0151 (1.00) |
0.0473 (1.00) |
0.0192 (1.00) |
1 (1.00) |
0.0036 (1.00) |
0.00506 (1.00) |
0.237 (1.00) |
10p loss | 32 (7%) | 458 |
0.515 (1.00) |
0.512 (1.00) |
0.449 (1.00) |
0.0261 (1.00) |
0.0146 (1.00) |
1 (1.00) |
0.803 (1.00) |
0.878 (1.00) |
1 (1.00) |
10q loss | 48 (10%) | 442 |
0.755 (1.00) |
0.276 (1.00) |
0.267 (1.00) |
0.0535 (1.00) |
0.00891 (1.00) |
0.622 (1.00) |
0.677 (1.00) |
0.644 (1.00) |
1 (1.00) |
11q loss | 7 (1%) | 483 |
0.153 (1.00) |
0.647 (1.00) |
0.43 (1.00) |
0.799 (1.00) |
0.264 (1.00) |
1 (1.00) |
1 (1.00) |
1 (1.00) |
|
13q loss | 31 (6%) | 459 |
0.00131 (0.786) |
0.0557 (1.00) |
0.333 (1.00) |
0.00419 (1.00) |
0.00143 (0.857) |
1 (1.00) |
0.0405 (1.00) |
0.0125 (1.00) |
1 (1.00) |
14q loss | 158 (32%) | 332 |
0.268 (1.00) |
0.235 (1.00) |
0.225 (1.00) |
0.508 (1.00) |
0.0242 (1.00) |
0.0688 (1.00) |
0.145 (1.00) |
0.03 (1.00) |
0.0393 (1.00) |
15q loss | 11 (2%) | 479 |
0.529 (1.00) |
0.048 (1.00) |
0.755 (1.00) |
0.406 (1.00) |
0.37 (1.00) |
0.683 (1.00) |
0.39 (1.00) |
1 (1.00) |
|
16q loss | 5 (1%) | 485 |
0.0898 (1.00) |
0.0723 (1.00) |
0.662 (1.00) |
0.00645 (1.00) |
1 (1.00) |
0.571 (1.00) |
0.0742 (1.00) |
1 (1.00) |
|
17p loss | 25 (5%) | 465 |
0.0405 (1.00) |
0.678 (1.00) |
0.287 (1.00) |
0.0473 (1.00) |
0.134 (1.00) |
1 (1.00) |
0.0402 (1.00) |
0.228 (1.00) |
0.231 (1.00) |
17q loss | 12 (2%) | 478 |
0.691 (1.00) |
0.817 (1.00) |
0.231 (1.00) |
0.0485 (1.00) |
1 (1.00) |
1 (1.00) |
0.471 (1.00) |
0.117 (1.00) |
|
18p loss | 45 (9%) | 445 |
0.208 (1.00) |
0.0622 (1.00) |
0.626 (1.00) |
0.518 (1.00) |
0.0868 (1.00) |
0.68 (1.00) |
0.197 (1.00) |
0.319 (1.00) |
0.384 (1.00) |
18q loss | 47 (10%) | 443 |
0.54 (1.00) |
0.144 (1.00) |
0.335 (1.00) |
0.518 (1.00) |
0.379 (1.00) |
0.692 (1.00) |
0.287 (1.00) |
0.626 (1.00) |
0.397 (1.00) |
19p loss | 4 (1%) | 486 |
0.000557 (0.337) |
0.901 (1.00) |
0.614 (1.00) |
0.0143 (1.00) |
1 (1.00) |
1 (1.00) |
0.0697 (1.00) |
1 (1.00) |
|
20p loss | 6 (1%) | 484 |
0.761 (1.00) |
0.957 (1.00) |
1 (1.00) |
0.881 (1.00) |
1 (1.00) |
1 (1.00) |
0.931 (1.00) |
1 (1.00) |
|
21q loss | 32 (7%) | 458 |
0.768 (1.00) |
0.974 (1.00) |
1 (1.00) |
0.00594 (1.00) |
0.361 (1.00) |
0.45 (1.00) |
0.177 (1.00) |
1 (1.00) |
|
22q loss | 11 (2%) | 479 |
0.232 (1.00) |
0.0464 (1.00) |
0.343 (1.00) |
0.274 (1.00) |
1 (1.00) |
1 (1.00) |
0.193 (1.00) |
1 (1.00) |
|
Xq loss | 7 (1%) | 483 |
0.504 (1.00) |
0.734 (1.00) |
0.102 (1.00) |
0.633 (1.00) |
1 (1.00) |
0.602 (1.00) |
0.577 (1.00) |
1 (1.00) |
P value = 0.000105 (t-test), Q value = 0.064
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 489 | 60.6 (12.2) |
5P GAIN MUTATED | 141 | 64.0 (11.9) |
5P GAIN WILD-TYPE | 348 | 59.3 (12.1) |
P value = 0.000273 (t-test), Q value = 0.16
nPatients | Mean (Std.Dev) | |
---|---|---|
ALL | 489 | 60.6 (12.2) |
5Q GAIN MUTATED | 154 | 63.6 (12.2) |
5Q GAIN WILD-TYPE | 335 | 59.3 (11.9) |
P value = 4.3e-05 (logrank test), Q value = 0.026
nPatients | nDeath | Duration Range (Median), Month | |
---|---|---|---|
ALL | 487 | 157 | 0.1 - 111.0 (34.6) |
11P LOSS MUTATED | 5 | 4 | 1.8 - 28.5 (12.1) |
11P LOSS WILD-TYPE | 482 | 153 | 0.1 - 111.0 (35.3) |
-
Mutation data file = broad_values_by_arm.mutsig.cluster.txt
-
Clinical data file = KIRC.clin.merged.picked.txt
-
Number of patients = 490
-
Number of significantly arm-level cnvs = 71
-
Number of selected clinical features = 9
-
Exclude genes that fewer than K tumors have mutations, K = 3
For survival clinical features, the Kaplan-Meier survival curves of tumors with and without gene mutations were plotted and the statistical significance P values were estimated by logrank test (Bland and Altman 2004) using the 'survdiff' function in R
For continuous numerical clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the clinical values between tumors with and without gene mutations using 't.test' function in R
For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.