This pipeline uses various statistical tests to identify miRs whose expression levels correlated to selected clinical features.
Testing the association between 530 miRs and 13 clinical features across 459 samples, statistically thresholded by Q value < 0.05, 6 clinical features related to at least one miRs.
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9 miRs correlated to 'PATHOLOGY.T.STAGE'.
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HSA-MIR-30C-2 , HSA-MIR-451 , HSA-MIR-101-2 , HSA-MIR-150 , HSA-MIR-146A , ...
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9 miRs correlated to 'PATHOLOGY.M.STAGE'.
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HSA-MIR-628 , HSA-MIR-21 , HSA-MIR-340 , HSA-MIR-660 , HSA-MIR-374A , ...
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2 miRs correlated to 'GENDER'.
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HSA-MIR-105-2 , HSA-MIR-105-1
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9 miRs correlated to 'HISTOLOGICAL.TYPE'.
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HSA-MIR-21 , HSA-MIR-194-1 , HSA-MIR-194-2 , HSA-MIR-200B , HSA-MIR-219-2 , ...
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1 miR correlated to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
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HSA-MIR-143
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4 miRs correlated to 'NUMBERPACKYEARSSMOKED'.
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HSA-MIR-339 , HSA-MIR-345 , HSA-MIR-210 , HSA-MIR-296
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No miRs correlated to 'Time to Death', 'AGE', 'NEOPLASM.DISEASESTAGE', 'PATHOLOGY.N.STAGE', 'KARNOFSKY.PERFORMANCE.SCORE', 'YEAROFTOBACCOSMOKINGONSET', and 'COMPLETENESS.OF.RESECTION'.
Complete statistical result table is provided in Supplement Table 1
Clinical feature | Statistical test | Significant miRs | Associated with | Associated with | ||
---|---|---|---|---|---|---|
Time to Death | Cox regression test | N=0 | ||||
AGE | Spearman correlation test | N=0 | ||||
NEOPLASM DISEASESTAGE | ANOVA test | N=0 | ||||
PATHOLOGY T STAGE | Spearman correlation test | N=9 | higher stage | N=0 | lower stage | N=9 |
PATHOLOGY N STAGE | Spearman correlation test | N=0 | ||||
PATHOLOGY M STAGE | ANOVA test | N=9 | ||||
GENDER | t test | N=2 | male | N=2 | female | N=0 |
KARNOFSKY PERFORMANCE SCORE | Spearman correlation test | N=0 | ||||
HISTOLOGICAL TYPE | ANOVA test | N=9 | ||||
RADIATIONS RADIATION REGIMENINDICATION | t test | N=1 | yes | N=0 | no | N=1 |
NUMBERPACKYEARSSMOKED | Spearman correlation test | N=4 | higher numberpackyearssmoked | N=4 | lower numberpackyearssmoked | N=0 |
YEAROFTOBACCOSMOKINGONSET | Spearman correlation test | N=0 | ||||
COMPLETENESS OF RESECTION | ANOVA test | N=0 |
Time to Death | Duration (Months) | 0-224 (median=13.2) |
censored | N = 308 | |
death | N = 119 | |
Significant markers | N = 0 |
AGE | Mean (SD) | 65.55 (9.7) |
Significant markers | N = 0 |
NEOPLASM.DISEASESTAGE | Labels | N |
STAGE I | 4 | |
STAGE IA | 111 | |
STAGE IB | 133 | |
STAGE IIA | 44 | |
STAGE IIB | 65 | |
STAGE IIIA | 68 | |
STAGE IIIB | 11 | |
STAGE IV | 22 | |
Significant markers | N = 0 |
PATHOLOGY.T.STAGE | Mean (SD) | 1.86 (0.73) |
N | ||
1 | 141 | |
2 | 256 | |
3 | 41 | |
4 | 18 | |
Significant markers | N = 9 | |
pos. correlated | 0 | |
neg. correlated | 9 |
SpearmanCorr | corrP | Q | |
---|---|---|---|
HSA-MIR-30C-2 | -0.2201 | 2.074e-06 | 0.0011 |
HSA-MIR-451 | -0.2067 | 8.567e-06 | 0.00453 |
HSA-MIR-101-2 | -0.2062 | 9.082e-06 | 0.0048 |
HSA-MIR-150 | -0.1998 | 1.719e-05 | 0.00906 |
HSA-MIR-146A | -0.1991 | 1.851e-05 | 0.00974 |
HSA-MIR-374B | -0.1946 | 2.872e-05 | 0.0151 |
HSA-MIR-342 | -0.1914 | 3.885e-05 | 0.0204 |
HSA-MIR-218-2 | -0.1888 | 4.955e-05 | 0.0259 |
HSA-MIR-486 | -0.1842 | 7.589e-05 | 0.0396 |
PATHOLOGY.N.STAGE | Mean (SD) | 0.51 (0.77) |
N | ||
0 | 292 | |
1 | 84 | |
2 | 70 | |
3 | 2 | |
Significant markers | N = 0 |
PATHOLOGY.M.STAGE | Labels | N |
M0 | 314 | |
M1 | 16 | |
M1A | 1 | |
M1B | 3 | |
MX | 121 | |
Significant markers | N = 9 |
ANOVA_P | Q | |
---|---|---|
HSA-MIR-628 | 5.953e-07 | 0.000316 |
HSA-MIR-21 | 3.943e-06 | 0.00209 |
HSA-MIR-340 | 1.565e-05 | 0.00826 |
HSA-MIR-660 | 1.738e-05 | 0.00916 |
HSA-MIR-374A | 1.746e-05 | 0.00918 |
HSA-MIR-532 | 2.258e-05 | 0.0119 |
HSA-MIR-1468 | 7.145e-05 | 0.0374 |
HSA-MIR-16-1 | 7.258e-05 | 0.038 |
HSA-MIR-424 | 7.635e-05 | 0.0399 |
GENDER | Labels | N |
FEMALE | 247 | |
MALE | 212 | |
Significant markers | N = 2 | |
Higher in MALE | 2 | |
Higher in FEMALE | 0 |
T(pos if higher in 'MALE') | ttestP | Q | AUC | |
---|---|---|---|---|
HSA-MIR-105-2 | 5.12 | 6.08e-07 | 0.000322 | 0.6705 |
HSA-MIR-105-1 | 4.21 | 3.512e-05 | 0.0186 | 0.648 |
No miR related to 'KARNOFSKY.PERFORMANCE.SCORE'.
KARNOFSKY.PERFORMANCE.SCORE | Mean (SD) | 83.16 (23) |
Significant markers | N = 0 |
HISTOLOGICAL.TYPE | Labels | N |
LUNG ACINAR ADENOCARCINOMA | 13 | |
LUNG ADENOCARCINOMA MIXED SUBTYPE | 92 | |
LUNG ADENOCARCINOMA- NOT OTHERWISE SPECIFIED (NOS) | 294 | |
LUNG BRONCHIOLOALVEOLAR CARCINOMA MUCINOUS | 4 | |
LUNG BRONCHIOLOALVEOLAR CARCINOMA NONMUCINOUS | 19 | |
LUNG CLEAR CELL ADENOCARCINOMA | 2 | |
LUNG MICROPAPILLARY ADENOCARCINOMA | 3 | |
LUNG MUCINOUS ADENOCARCINOMA | 2 | |
LUNG PAPILLARY ADENOCARCINOMA | 18 | |
LUNG SIGNET RING ADENOCARCINOMA | 1 | |
LUNG SOLID PATTERN PREDOMINANT ADENOCARCINOMA | 4 | |
MUCINOUS (COLLOID) CARCINOMA | 7 | |
Significant markers | N = 9 |
ANOVA_P | Q | |
---|---|---|
HSA-MIR-21 | 2.794e-08 | 1.48e-05 |
HSA-MIR-194-1 | 1.404e-07 | 7.43e-05 |
HSA-MIR-194-2 | 1.817e-07 | 9.59e-05 |
HSA-MIR-200B | 1.92e-05 | 0.0101 |
HSA-MIR-219-2 | 4.935e-05 | 0.026 |
HSA-MIR-192 | 5.061e-05 | 0.0266 |
HSA-MIR-656 | 6.214e-05 | 0.0326 |
HSA-MIR-200A | 8.222e-05 | 0.043 |
HSA-MIR-532 | 9.519e-05 | 0.0497 |
One miR related to 'RADIATIONS.RADIATION.REGIMENINDICATION'.
RADIATIONS.RADIATION.REGIMENINDICATION | Labels | N |
NO | 20 | |
YES | 439 | |
Significant markers | N = 1 | |
Higher in YES | 0 | |
Higher in NO | 1 |
T(pos if higher in 'YES') | ttestP | Q | AUC | |
---|---|---|---|---|
HSA-MIR-143 | -4.97 | 5.264e-05 | 0.0278 | 0.7436 |
NUMBERPACKYEARSSMOKED | Mean (SD) | 42.25 (27) |
Significant markers | N = 4 | |
pos. correlated | 4 | |
neg. correlated | 0 |
SpearmanCorr | corrP | Q | |
---|---|---|---|
HSA-MIR-339 | 0.2557 | 4.463e-06 | 0.00237 |
HSA-MIR-345 | 0.2548 | 4.802e-06 | 0.00254 |
HSA-MIR-210 | 0.2543 | 5.014e-06 | 0.00265 |
HSA-MIR-296 | 0.2491 | 8.231e-06 | 0.00434 |
YEAROFTOBACCOSMOKINGONSET | Mean (SD) | 1964.63 (12) |
Significant markers | N = 0 |
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Expresson data file = LUAD-TP.miRseq_RPKM_log2.txt
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Clinical data file = LUAD-TP.merged_data.txt
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Number of patients = 459
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Number of miRs = 530
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Number of clinical features = 13
For survival clinical features, Wald's test in univariate Cox regression analysis with proportional hazards model (Andersen and Gill 1982) was used to estimate the P values using the 'coxph' function in R. Kaplan-Meier survival curves were plot using the four quartile subgroups of patients based on expression levels
For continuous numerical clinical features, Spearman's rank correlation coefficients (Spearman 1904) and two-tailed P values were estimated using 'cor.test' function in R
For multi-class clinical features (ordinal or nominal), one-way analysis of variance (Howell 2002) was applied to compare the log2-expression levels between different clinical classes using 'anova' function in R
For two-class clinical features, two-tailed Student's t test with unequal variance (Lehmann and Romano 2005) was applied to compare the log2-expression levels between the two clinical classes using 't.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
In addition to the links below, the full results of the analysis summarized in this report can also be downloaded programmatically using firehose_get, or interactively from either the Broad GDAC website or TCGA Data Coordination Center Portal.