(NRAS_Hotspot_Mutants cohort)
This pipeline computes the correlation between significant copy number variation (cnv) genes and molecular subtypes.
Testing the association between copy number variation of 33 peak regions and 8 molecular subtypes across 62 patients, 6 significant findings detected with Q value < 0.25.
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Amp Peak 8(12q15) cnvs correlated to 'CN_CNMF'.
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Del Peak 3(5q31.1) cnvs correlated to 'METHLYATION_CNMF'.
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Del Peak 4(5q31.1) cnvs correlated to 'METHLYATION_CNMF'.
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Del Peak 5(6q16.3) cnvs correlated to 'CN_CNMF'.
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Del Peak 6(6q22.31) cnvs correlated to 'CN_CNMF'.
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Del Peak 7(6q26) cnvs correlated to 'CN_CNMF'.
Molecular subtypes |
CN CNMF |
METHLYATION CNMF |
RPPA CNMF |
RPPA CHIERARCHICAL |
MRNASEQ CNMF |
MRNASEQ CHIERARCHICAL |
MIRSEQ CNMF |
MIRSEQ CHIERARCHICAL |
||
nCNV (%) | nWild-Type | Fisher's exact test | Fisher's exact test | Fisher's exact test | Chi-square test | Fisher's exact test | Fisher's exact test | Fisher's exact test | Fisher's exact test | |
Amp Peak 8(12q15) | 9 (15%) | 53 |
0.000105 (0.0277) |
0.905 (1.00) |
0.407 (1.00) |
0.431 (1.00) |
0.445 (1.00) |
0.537 (1.00) |
0.106 (1.00) |
0.13 (1.00) |
Del Peak 3(5q31 1) | 28 (45%) | 34 |
0.00169 (0.431) |
0.000127 (0.0333) |
1 (1.00) |
0.694 (1.00) |
0.18 (1.00) |
0.0691 (1.00) |
0.862 (1.00) |
0.196 (1.00) |
Del Peak 4(5q31 1) | 28 (45%) | 34 |
0.00169 (0.431) |
0.000127 (0.0333) |
1 (1.00) |
0.694 (1.00) |
0.18 (1.00) |
0.0691 (1.00) |
0.862 (1.00) |
0.196 (1.00) |
Del Peak 5(6q16 3) | 42 (68%) | 20 |
7.16e-06 (0.00189) |
0.889 (1.00) |
0.741 (1.00) |
0.822 (1.00) |
0.0401 (1.00) |
0.0408 (1.00) |
0.106 (1.00) |
0.01 (1.00) |
Del Peak 6(6q22 31) | 43 (69%) | 19 |
0.000182 (0.0472) |
0.885 (1.00) |
0.501 (1.00) |
0.907 (1.00) |
0.163 (1.00) |
0.227 (1.00) |
0.0656 (1.00) |
0.00471 (1.00) |
Del Peak 7(6q26) | 44 (71%) | 18 |
0.000126 (0.0329) |
0.685 (1.00) |
0.501 (1.00) |
0.907 (1.00) |
0.26 (1.00) |
0.326 (1.00) |
0.0349 (1.00) |
0.00168 (0.43) |
Amp Peak 1(1p13 2) | 21 (34%) | 41 |
0.287 (1.00) |
0.561 (1.00) |
1 (1.00) |
0.71 (1.00) |
0.792 (1.00) |
0.353 (1.00) |
0.626 (1.00) |
0.0666 (1.00) |
Amp Peak 2(1q31 2) | 37 (60%) | 25 |
0.0271 (1.00) |
0.65 (1.00) |
0.748 (1.00) |
0.487 (1.00) |
0.204 (1.00) |
0.64 (1.00) |
0.25 (1.00) |
0.332 (1.00) |
Amp Peak 3(1q44) | 38 (61%) | 24 |
0.0284 (1.00) |
0.415 (1.00) |
0.748 (1.00) |
0.372 (1.00) |
0.166 (1.00) |
0.636 (1.00) |
0.257 (1.00) |
0.163 (1.00) |
Amp Peak 4(5p15 33) | 13 (21%) | 49 |
0.839 (1.00) |
0.859 (1.00) |
1 (1.00) |
0.306 (1.00) |
0.498 (1.00) |
0.67 (1.00) |
0.689 (1.00) |
0.719 (1.00) |
Amp Peak 5(6p25 1) | 46 (74%) | 16 |
0.00463 (1.00) |
0.0421 (1.00) |
0.716 (1.00) |
0.966 (1.00) |
0.273 (1.00) |
0.81 (1.00) |
0.041 (1.00) |
0.00213 (0.539) |
Amp Peak 6(7p21 3) | 22 (35%) | 40 |
0.228 (1.00) |
0.167 (1.00) |
0.501 (1.00) |
0.508 (1.00) |
0.4 (1.00) |
0.706 (1.00) |
0.00129 (0.332) |
0.026 (1.00) |
Amp Peak 7(11q14 1) | 10 (16%) | 52 |
1 (1.00) |
0.911 (1.00) |
1 (1.00) |
0.352 (1.00) |
1 (1.00) |
0.904 (1.00) |
0.289 (1.00) |
0.673 (1.00) |
Amp Peak 9(15q26 1) | 18 (29%) | 44 |
0.12 (1.00) |
0.602 (1.00) |
0.451 (1.00) |
0.539 (1.00) |
0.226 (1.00) |
0.326 (1.00) |
0.122 (1.00) |
0.91 (1.00) |
Amp Peak 10(17q25 3) | 25 (40%) | 37 |
0.0594 (1.00) |
0.421 (1.00) |
1 (1.00) |
0.0979 (1.00) |
0.467 (1.00) |
0.64 (1.00) |
0.172 (1.00) |
0.0567 (1.00) |
Amp Peak 11(20q13 2) | 28 (45%) | 34 |
0.0122 (1.00) |
0.0883 (1.00) |
1 (1.00) |
0.469 (1.00) |
0.53 (1.00) |
0.579 (1.00) |
0.303 (1.00) |
0.46 (1.00) |
Amp Peak 12(22q13 2) | 23 (37%) | 39 |
0.00304 (0.767) |
0.573 (1.00) |
0.731 (1.00) |
0.274 (1.00) |
0.895 (1.00) |
0.595 (1.00) |
0.183 (1.00) |
0.135 (1.00) |
Del Peak 1(1p36 13) | 15 (24%) | 47 |
0.748 (1.00) |
0.0115 (1.00) |
0.48 (1.00) |
0.1 (1.00) |
0.37 (1.00) |
0.0934 (1.00) |
0.889 (1.00) |
1 (1.00) |
Del Peak 2(4q34 3) | 15 (24%) | 47 |
0.00807 (1.00) |
0.569 (1.00) |
0.0436 (1.00) |
0.0593 (1.00) |
0.0914 (1.00) |
0.0638 (1.00) |
0.0134 (1.00) |
0.00746 (1.00) |
Del Peak 8(7q31 1) | 11 (18%) | 51 |
0.585 (1.00) |
0.162 (1.00) |
1 (1.00) |
0.128 (1.00) |
0.778 (1.00) |
0.757 (1.00) |
0.649 (1.00) |
0.547 (1.00) |
Del Peak 9(9p23) | 47 (76%) | 15 |
0.176 (1.00) |
0.176 (1.00) |
1 (1.00) |
0.139 (1.00) |
0.239 (1.00) |
0.42 (1.00) |
0.889 (1.00) |
0.116 (1.00) |
Del Peak 10(9p21 3) | 52 (84%) | 10 |
0.0842 (1.00) |
0.517 (1.00) |
1 (1.00) |
0.371 (1.00) |
0.0913 (1.00) |
0.146 (1.00) |
0.722 (1.00) |
0.12 (1.00) |
Del Peak 11(10p15 3) | 34 (55%) | 28 |
0.0477 (1.00) |
0.176 (1.00) |
1 (1.00) |
0.524 (1.00) |
0.145 (1.00) |
0.226 (1.00) |
0.59 (1.00) |
0.231 (1.00) |
Del Peak 12(10q23 31) | 36 (58%) | 26 |
0.202 (1.00) |
0.69 (1.00) |
0.752 (1.00) |
0.201 (1.00) |
0.149 (1.00) |
0.247 (1.00) |
0.321 (1.00) |
0.0853 (1.00) |
Del Peak 13(11q24 2) | 42 (68%) | 20 |
0.484 (1.00) |
0.661 (1.00) |
0.501 (1.00) |
0.49 (1.00) |
0.786 (1.00) |
0.693 (1.00) |
0.582 (1.00) |
0.454 (1.00) |
Del Peak 14(12p12 3) | 14 (23%) | 48 |
0.766 (1.00) |
0.219 (1.00) |
0.127 (1.00) |
0.245 (1.00) |
0.59 (1.00) |
1 (1.00) |
0.742 (1.00) |
0.725 (1.00) |
Del Peak 15(12q23 3) | 21 (34%) | 41 |
0.00486 (1.00) |
0.22 (1.00) |
0.501 (1.00) |
0.673 (1.00) |
0.44 (1.00) |
0.738 (1.00) |
0.805 (1.00) |
0.192 (1.00) |
Del Peak 16(13q14 2) | 9 (15%) | 53 |
0.389 (1.00) |
0.0608 (1.00) |
1 (1.00) |
0.419 (1.00) |
0.272 (1.00) |
0.537 (1.00) |
0.16 (1.00) |
1 (1.00) |
Del Peak 17(14q31 3) | 25 (40%) | 37 |
0.00542 (1.00) |
0.806 (1.00) |
0.748 (1.00) |
0.0325 (1.00) |
0.283 (1.00) |
0.169 (1.00) |
0.808 (1.00) |
0.0717 (1.00) |
Del Peak 18(15q13 3) | 19 (31%) | 43 |
0.274 (1.00) |
0.419 (1.00) |
0.731 (1.00) |
0.805 (1.00) |
0.834 (1.00) |
0.881 (1.00) |
0.258 (1.00) |
0.148 (1.00) |
Del Peak 19(16p13 3) | 12 (19%) | 50 |
0.807 (1.00) |
1 (1.00) |
0.0915 (1.00) |
0.623 (1.00) |
0.778 (1.00) |
0.772 (1.00) |
0.243 (1.00) |
0.0756 (1.00) |
Del Peak 20(16q23 3) | 20 (32%) | 42 |
0.964 (1.00) |
0.661 (1.00) |
0.501 (1.00) |
0.93 (1.00) |
0.786 (1.00) |
0.83 (1.00) |
0.716 (1.00) |
0.348 (1.00) |
Del Peak 21(21q11 2) | 15 (24%) | 47 |
0.284 (1.00) |
0.152 (1.00) |
1 (1.00) |
0.473 (1.00) |
0.00112 (0.289) |
0.111 (1.00) |
0.889 (1.00) |
1 (1.00) |
P value = 0.000105 (Fisher's exact test), Q value = 0.028
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 16 | 12 | 20 | 14 |
AMP PEAK 8(12Q15) MUTATED | 8 | 0 | 1 | 0 |
AMP PEAK 8(12Q15) WILD-TYPE | 8 | 12 | 19 | 14 |
P value = 0.000127 (Fisher's exact test), Q value = 0.033
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 18 | 21 | 23 |
DEL PEAK 3(5Q31.1) MUTATED | 1 | 13 | 14 |
DEL PEAK 3(5Q31.1) WILD-TYPE | 17 | 8 | 9 |
P value = 0.000127 (Fisher's exact test), Q value = 0.033
nPatients | CLUS_1 | CLUS_2 | CLUS_3 |
---|---|---|---|
ALL | 18 | 21 | 23 |
DEL PEAK 4(5Q31.1) MUTATED | 1 | 13 | 14 |
DEL PEAK 4(5Q31.1) WILD-TYPE | 17 | 8 | 9 |
P value = 7.16e-06 (Fisher's exact test), Q value = 0.0019
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 16 | 12 | 20 | 14 |
DEL PEAK 5(6Q16.3) MUTATED | 15 | 1 | 16 | 10 |
DEL PEAK 5(6Q16.3) WILD-TYPE | 1 | 11 | 4 | 4 |
P value = 0.000182 (Fisher's exact test), Q value = 0.047
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 16 | 12 | 20 | 14 |
DEL PEAK 6(6Q22.31) MUTATED | 14 | 2 | 17 | 10 |
DEL PEAK 6(6Q22.31) WILD-TYPE | 2 | 10 | 3 | 4 |
P value = 0.000126 (Fisher's exact test), Q value = 0.033
nPatients | CLUS_1 | CLUS_2 | CLUS_3 | CLUS_4 |
---|---|---|---|---|
ALL | 16 | 12 | 20 | 14 |
DEL PEAK 7(6Q26) MUTATED | 14 | 2 | 17 | 11 |
DEL PEAK 7(6Q26) WILD-TYPE | 2 | 10 | 3 | 3 |
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Copy number data file = All Lesions File (all_lesions.conf_##.txt, where ## is the confidence level). The all lesions file is from GISTIC pipeline and summarizes the results from the GISTIC run. It contains data about the significant regions of amplification and deletion as well as which samples are amplified or deleted in each of these regions. The identified regions are listed down the first column, and the samples are listed across the first row, starting in column 10.
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Molecular subtype file = SKCM-NRAS_Hotspot_Mutants.transferedmergedcluster.txt
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Number of patients = 62
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Number of copy number variation regions = 33
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Number of molecular subtypes = 8
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Exclude regions that fewer than K tumors have alterations, K = 3
For binary or multi-class clinical features (nominal or ordinal), two-tailed Fisher's exact tests (Fisher 1922) were used to estimate the P values using the 'fisher.test' function in R
For multi-class clinical features (nominal or ordinal), Chi-square tests (Greenwood and Nikulin 1996) were used to estimate the P values using the 'chisq.test' function in R
For multiple hypothesis correction, Q value is the False Discovery Rate (FDR) analogue of the P value (Benjamini and Hochberg 1995), defined as the minimum FDR at which the test may be called significant. We used the 'Benjamini and Hochberg' method of 'p.adjust' function in R to convert P values into Q values.
This is an experimental feature. The full results of the analysis summarized in this report can be downloaded from the TCGA Data Coordination Center.